简介：Asequentialstatisticalapproachwasappliedtooptimizingthefermentationmediumofepothilones(Epos)productionbymeansofamutantwhichwasobtainedbytreatingpolyangiumcellulosumATCC15384withnitriteandultraviolet.Theeffectsofdifferentcarbonsourcesandnitrogensourcesonthefermentationmediumweretested,andthesuitableoneswereselected.Thenauniformdesignwasemployedtodesigntheexperiments.Alinearmodelwasdevelopedforidentifyingthesignificantcomponentsinfermentationmedium,whileathirddegreepolynomialmodelwasusedforstudyingtherelationshipbetweentheconcentrationofthecomponentsinfermentationmediumandtheyieldofEpos(YEPs).Apatternsearchmethodwasusedforsearchingtheoptimumfermentationmediuminthetestspace,whichwasasfollows(g/L):potassiumnitrate8.00,soybeanpeptone17.60,potassiumhydrogenphosphate1.00,beefextraction6.46,yeastextraction1.00,calciumchloride0.25,sodiumchloride1.00andferricchloride0.02.TheoptimumfermentationmediumwasexpectedtoresultinayieldofEpos(YEPs)of2.48mg/L.ThevalidationexperimentswiththeoptimummediumwereperformedintriplicateandtheaverageyieldofEposwas2.45mg/Lwhichwas7.78timeshigherthanthatofEpospreparedwithoutoptimization.

简介：Polyacrylonitrilebeads(PAN)cysteine(CS)wassynthesizedfrompolyacrylonitrilebeads(PAN)andcysteine(CS).ThecontentofthefunctionalgroupandthepercentageconversionofthefunctionalgroupofPAN-CSpreparedundertheoptimumconditionusingresponsesurfacemethodology(RSM)forthefirstattemptwere3.22mmol/gand35.78%.ThestructurewascharacterizedbyET-IRandelementalanalysis.TheadsorptionpropertiesoftheresinforCu(Ⅱ)wereinvestigatedbybatchandcolumnexperiments.BatchadsorptionresultssuggestedthatPAN-CShadhigheradsorptioncapabilityforCu(Ⅱ)thanothermetalionsandmaximumsaturatedadsorptioncapacitywas184.7mg/g.TheresinanditsmetalcomplexeswerestudiedbyFT-IR.Furthermore,theresincanbeelutedeasilyusing1mol/LHC1.PAN-CScanprovideapotentialapplicationforselectiveremovalofcopperfromwastesolution.

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