简介:Asequentialstatisticalapproachwasappliedtooptimizingthefermentationmediumofepothilones(Epos)productionbymeansofamutantwhichwasobtainedbytreatingpolyangiumcellulosumATCC15384withnitriteandultraviolet.Theeffectsofdifferentcarbonsourcesandnitrogensourcesonthefermentationmediumweretested,andthesuitableoneswereselected.Thenauniformdesignwasemployedtodesigntheexperiments.Alinearmodelwasdevelopedforidentifyingthesignificantcomponentsinfermentationmedium,whileathirddegreepolynomialmodelwasusedforstudyingtherelationshipbetweentheconcentrationofthecomponentsinfermentationmediumandtheyieldofEpos(YEPs).Apatternsearchmethodwasusedforsearchingtheoptimumfermentationmediuminthetestspace,whichwasasfollows(g/L):potassiumnitrate8.00,soybeanpeptone17.60,potassiumhydrogenphosphate1.00,beefextraction6.46,yeastextraction1.00,calciumchloride0.25,sodiumchloride1.00andferricchloride0.02.TheoptimumfermentationmediumwasexpectedtoresultinayieldofEpos(YEPs)of2.48mg/L.ThevalidationexperimentswiththeoptimummediumwereperformedintriplicateandtheaverageyieldofEposwas2.45mg/Lwhichwas7.78timeshigherthanthatofEpospreparedwithoutoptimization.
简介:ThecatalyticoxidationofcyclohexenetocyclohexanoneusingPd(OAc)2/HQ/FePcwasinvestigatedinanacidicaqueoussolutionofacetonitrile.TheroleofeachcomponentofthissystemintheoxidationofcyclohexenewasexploredbymeansofUV-VIS,IR,XPSspectroscopyandcyclicvoltammetry,respectively.Basedontheexperimentalresults,themechanismoftheoxidationofcyelohexenecatalyzedbyPd(OAc)2/HQ/FePcwaselucidated.
简介:CarbonylationofAllylChlorideCatalyzedbyPdCl2(TPPTS)2inTwo-phaseMediumCarbonylationofAllylChlorideCatalyzedbyPdCl2(TPPTS)2in...