简介：ToconstructandexpressthefusionproteinStx2B-IntiminC300ofEHEC0157:H7,andtofurtherinvestigateitsimmunoprophyiacticpotential,thegeneofStx2B(stx2b)fromEHEC0157:H7chromosomewasclonedintopMD18-Tvector.Thereafter,theamplifiedgenewasclonedintoprokaryoticexpressionplasmidpET-28a(+)-eaeC300,whichwasconstructedpreviously.TherecombinantpasmidpET-28a(+)-stx2b-eaeC300wastransformedintoE.coliBL21(DE3).Afterinducement,theproteinStx2B-IntiminC300wassuccessfullyexpressedandanalyzedwithsodiumdodecylsulfatepolyacrylamidegelelectrophoresis(SDS-PAGE),WesternblottingandN-terminalaminoacidresidualsequencing.Toevaluateitsimmunoprophyiacticpotential,itwasprimarilypurifiedbyion-exchangechromatographyandinjectedinto30BALB/cmicewithAl(OH)3inthesubscapularregion.Tendaysafterthelastboostervaccination,20micewereattackedwithEHEC0157:H7lysateandtheprotectiveefficacywasobserved.Inthepresentstudy,thegeneofStx2B-IntiminC300wassuccessfullyclonedintopET-28a(+)vector.TheresultsofSDS-PAGEandWesternblottingassayshowedthatthefusionproteinwassuccessfullyexpressedintheinclusionbodyform,accountingfor25%oftotalexpressionproducts,anditsmolecularweightwasabout43kDa.TheresultoftheN-terminalaminoacidresidualsequencingshowedthatitwasidenticaltothatofthemoleculardesigned.Thepuritywasabout75%afterprimarypurification.AnimaltestsrevealedthatthefusionproteinStx2B-IntiminC300haselicitedhightiterofprotectiveantibodyrelatively.TheseresultsdemonstratethatthefusionproteinStx2B-IntiminC300issuccessfullyexpressedinprokaryoticexpressionsystemandshowscertainimmunoprophyiacticpotential.

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简介：摘要：目的：评价在诊断异位妊娠中应用经腹部B超与经阴道B超的价值。方法：以92例于本医院（常州市武进中医医院）功能科进行检查的异位妊娠患者为观察对象，观察进行时间是2019年3月到2020年4月，依据随机数字表法结果将所有对象平均分成两组（实验组进行经阴道B超，对照组进行经腹部B超），各有患者46例。比照两组的检查准确率与附件检出情况。结果：与对照组比照，实验组患者的检查准确率与各附件检出率均更高，均存在显著差异（P﹤0.05）。结论：相比于经腹部B超，在诊断异位妊娠中应用经阴道B超的检查效果更为理想，其检查准确率与各附件检出率均更高，具有推广应用的意义。

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简介：摘要：目的：针对出现在妇产科的急腹症病人进行探究，分析结合腹部B超、阴道B超两种方式进行检测的实际效果。方法：我们此次实验共选择了120位病人进行探究，她们均来自妇产科且患有急腹症。借助两种检测方式对她们进行检查，之后分析腹部B超、阴道B超以及两种方式结合检测的结果，并对三者进行深入比较。结果：120位病人经过精确诊断后得出结果：黄体囊肿破裂、急性盆腔炎、卵巢肿瘤蒂扭转、异位妊娠的人数分别为17、13、22、68。将以上结论作为判断依据，据阴道B超诊断结果分析，120位病人中有20位病人的诊断结果有误，诊断准确率83.33%。据腹部B超诊断结果分析，120位病人中有30位病人的诊断结果错误，准确率为75%。据二者结合检测结果分析，有10位病人的诊断结果出现错误，准确率为91.67%。从这一结果来看，两种检测方式结合的检测效果要远比单一的检测结果更为准确。结论：在妇产科中，当为疑似患有急腹症的病人进行诊断时，不要仅采用腹部B超或者阴道B超进行检测，二者要结合使用才能提高检测的正确率，为更多的病人提供帮助。

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简介：Inordertoinvestigatetheimmtmogenicityofthecontrolled-releasemicroencapsulatedhepatitisBvaccineinmice,polyethyleneglycol-poly-dl-lactide(PELA)microsphereswithentrappedHSsAgwerepreparedbydoubleemulsionW/O/Wbasedonsolventextractionmethods.BALB/cmicewereimmunizedwiththeencapsulatedvaccinebyoralfeedingorinjection.Bloodsampleswerecollectedat8^th,10^th,14^thand24^thweeks,respectively,andthelevelsofantibodyresponseweredetectedbyEI.ISA.Itwasfoundthatthescanningelectronmicroscopyshowedthepreparedmicrosphereshadsmoothandsphericalsurface,suitableforvaccinedelivery.Twogroupsofmiceorallyfedwiththeencapsulatedorconventionalrecombinantvaccines,respectively,theresereshowednoobviousdifferenceintheIgGlevels.At14^thweek,thegroupinjectedwithasingledoseofencapsulatedvaccinehadasimilarlevelofIgGresponsetothegroupinjectedwithtwodosesoftherecombinationvaccine.At24^thweek,theIgGlevelsofthegroupinjectedwithtwodosesofencapsulatedvaccinewerehigherthanthoseofthegroupinjectedwithtwodosesoftherecombinationvaccine.ItconcludesthatControlled-releasemicroencapsulatedhepatitisBvaccinepossessesthefeatureofslowlyreleasinginv/voandlongtimesimmtmogenicity.

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简介：HepatitisBvaccine,asthefirsthigh-effectiverecombinantcommercialvaccine,wassuccessfullydevelopedintheearly1980s.Sincethen,differentopinionshaveoccurredonthequalityofvaccineswithrapiddevelopmentoftargetgeneselecting,antigenexpressionsystem,andqualityevaluation.DifferentantigensofhepatitisBvaccinesarederivedfromdifferentexpressionsystem,andtherearealsosomedifferencesonmanufactureprocedureorglycosylateddegreeofantigen.

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