简介：InordertostudyFcαRⅠmediatedphagocytosisotlgAimmunecomplexesbyU937cells,antigen8.9NIP/BSAwaslabeledwithFITCandreactedwithanti-NIPIgAoranti-NIPIgGantibodytoformimmunecomplexes(ICs).Theywerethenincubatedwithphorbol12-myristateB-acetate(PMA)stimulatedU937cells.ThephagocytosedICswerequantifiedbyflowcytometry.TheresultswasthattheexpressionofFcαRⅠonU937cellswashigherthanthatofFcγRⅠ,FcyRⅡandFcγRⅢ.AfterstimulationbyPMA,expressionofFcαRⅠonU937cellswasmarkedlyupregulatedandthephagocytosisofIgAICswasenhanced.FcαRⅠmediatedspecificIgAphagocytosiswasstrongerthanFcγRⅠandFcγRⅡmediatedIgGphagocytosis.Complementreceptors,CR1andCR3,enhancedU937cellphagocytosisofIgAICs.ItconcludesthatFcγRⅠmediatedstrongphagocytosisofIgAICs.

简介：【摘要】目的：分析早期糖尿病肾病患者接受Cysc联合U-mAlb检测的效果。方法：从2020年1月-2022年1月期间因早期糖尿病肾病入院的患者内随机选择62例作为实验组，选择同期健康的62例体检者作为对照组，对其实施联合检测，对比相关数据。结果：实验组患者生化指标显著高于对照组，P＜0.05。此外，实验组患者的生化指标阳性检出率显著高于对照组，P＜0.05。结论：对糖尿病肾病患者实施Cysc联合U-mAlb检测效果较好，可以为医生提供更多诊断数据。

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简介：TheproteomicsofthedifferentialproteinexpressionsinhumangliomacelllineU251cellsinfectedwithhumancytomegalovirus(HCMV)wasinvestigatedattheproteinlevelbyusingthesurfaceenhancedlaserdesorption/ionization(SELDI)proteinchipsysteminordertodevelopamethodofstudyforthepathogenesisofHCMVinfection.Inthisstudy,theculturedU251cellswereinfectedwithHC-MVingoodconditionandthesupernatantsoflysatesandtheextracellularfluidsofthecultivatedinfect-edcellswerequantitativelydefinedfortheexpressedproteins.TheproteomicsofthedifferentialproteinexpressionincellsbeforeandafterinfectionwasanalyzedbyWCX2arraysontheproteinchipreader.Itwasdemonstratedthatthecytopathiceffectsofinfectedcellsappearedonthe5thdayafterinfection,however,thedifferentialproteinexpressionwasevidentat6hafterinfectionasrevealedbyRT-PCRandmassspectrometry.Theproteinpeakscapturedfromdifferentbatchesofsamples,fromthesamesampledetectedwithdifferentarraysorforthedifferentlimeswereallequivalent.Withthemolecularweightrangefrom2000Dato3000Da,chipcaptured82peaksfromtheintracellularfluidsand11proteinpeakfromthecellularfluidinwhichcomparedwiththecontrolgroup,theproteinpeakswithmolecularweightof13536.3Da,10046.1Daand17106.2Dawereclosetothoseofβ-amyloidpro-tein,caspase-1precursorandLPS-inducedTNF-αfactorrespectively,whichshowedbriefup-regulation4hafterinfection,andcontinuedtoraise48hlater.Theseresultsinferthattheseproteinsmaybere-latedtotheapoptosisinducedbyHCMVinfection,thussuggestingthattheapeptosisinducedbyHC-MVinfectionmayplayaroleinthepathogenesisofHCMVinfection.