简介：Thedot-immunobindingassaywasappliedtoinvestigatethecharacteristicsofchickeneggyolkantibodies.Thismethodofassaywasprovedtobearapidandsimplemethodtodemonstrateandcharacterizetheegg-yolkantibodyIgYincomparisonwiththetraditionalELISAassay.ByusingtheBandScansoftware,thegrayscalevalueofdotsandthebackgroundcouldbedetermined.Accordingtotheintensityofdots(grayscalevalue)comparedtothestandardsampleof10μg,howmuchIgYremainedcanbedeterminedinashortertime.

简介：TherelationshipbetweenembBmutationofMycobacteriumtuberculosisandethambutol(EMB)resistanceoftheclinicalisolatesoftuberculouspatientsinChinawasinvestigatedbyreversedotblothybridization(RDBH)inadditiontoevaluatingtheclinicalvaluewithapplicationofPCR-RDBHtechniquetodetectEMBresistance.Inthepresentstudy,thegenotypesofthe258bpfragmentsofembBgenesfrom196clinicalisolatesofM.tuberculosiswereanalysedwithRDBHandDNAsequencing.Itwasdemonstratedthat60outof91phenotypicallyEMB-resistantisolates(65.9%)showed5typesofmissensemutationsatcodon306ofembBgene,resultinginthereplacementoftheMetresidueofthewildtypestrainwithVal,IleorLeuresidues.Inthesemutations,theGTPmutation(38/91,41.8%)andtheATAmutation(16/91,17.6%)werethemostencounteredgenotypes.TheembBmutationatcodon306couldalsobefoundin69isolatesofphenotypicallyEMB-sensitivebutresistanttootheranti-tuberculousdrugs,butnosuchgenemutationcouldbefoundin36strainsofdrug-sensitiveisolates.Meanwhile,theconcordancewiththeresultsofDNAsequencingforonewide-typeprobeand5probesforspecificmutationswas100%.ItwasconcludedthattheEMB-resistanceoccurringinmostM.tuberculosisisduetoappearanceofembBmutationatcodon306,andthePCR-RDBHassaywasprovedtobearapid,simpleandreliablemethodforthedetectionofgenemutations,whichmightbeagoodalternativeforthedrug-resistancescreening.

简介：【 摘要 】 目的 观察在诊断梅毒螺旋体感染中应用 ELISA法的临床作用。 方法 在我院 2018 年 12 月 -2019 年 12 月间收治的 梅毒螺旋体感染患者中随机选取 118 例为研究对象，所有患者均完成 ELISA法检查与甲基胺红不加热血清试验检查。统计 118 例患者在不同检查方式下的检查结果，并进行统计学分析。 结果 ELISA法检查后的诊断准确率、敏感性、特异性结果均显著优于甲基胺红不加热血清试验检查后的诊断准确率、敏感性、特异性结果（ P<0.05 ），数据结果对比有意义。 结论 在诊断梅毒螺旋体感染中应用 ELISA法的临床作用非常显著，具有较高的临床诊断准确率，有利于梅毒的诊断。并且也具有较高的特异性、敏感性，临床推广价值显著。