简介：IntroductionAsIreflectbackonmytimeasaneditor,Irecallafewissuesthatbotheredmerelatingtopublicationsinmedicaljournals,e.g.Impactfactorandtherelationshipofeditorialsandself-citationontheimpactfactorandacceptanceratesfororiginalpublications.Whatfollowsaresomethoughtsonthesubject.

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简介：BacgroundPercutaneouscoronaryintervention(PCI)hasbecomeoneofthemosteffectivetreatmentsincoronaryheartdisease(CHD).However,thebottleneckproblemofPCIisthein-stentrestenosis(ISR).TheaimofthisstudywastoexploretheeffectsofastragalosideIV(ASTIV)onsuppressionofintimalhyperplasiamodulationoftheexpressionofbasicfibroblastgrowthfactor(b-FGF)inaratcarotidarteryballooninjurymodel.MethodsFiftyhealthymaleSprague-Dawley(SD)ratswererandomlydividedintofivegroups:asham-operationgroup(sham),amodelgroup(model),andthreeastragalosideIV-treatedgroups.Threedaysbeforethesurgery,1%carboxymethylcellulose(CMC)orASTIV(20,40or60mg·kg~(-1)·d~(-1))wasintragastricallyadministeredintoshamor3astragaloside-treatedgroupsonceadayfor17days.Hematoxylin-elsinstainingwascarriedouttodeterminethepathomorphologicalchangesandtheneointimalandmediaarearatio.Immunohistochemistrystainingwasperformedtomeasuretheexpressionsofproliferatingcellnuclearantigen(PCNA)andbasicfibrolastgrowthfactor(b-FGF).PCNAandb-FGFwereanalyzedwithIamage-ProPlus.Results(1)Thecarotidarteryintimalhyperplasiaintheratsofmodelwassimilartolumenstenosis.Comparedwiththeshamoperationgroup,theareaofthenewintimaandtheratiooftheintimatomedia(I/M)wereincreasedandthelumenareawasdecreased(P<0.01)inthemodelgroup.AstragalosideIVincreasedthelumenintimaldimensionanddecreasedtheareaofnewintimaandtheratioofintimatomediainadose-dependentmanner.(2)Comparedwiththesham-operationgroup,theexpressionsofPCNAandb-FGFincarotidarteryofmodelgroupweresignificantlyincreased(P<0.01).ASTIVdecreasedexpressionsofPCNAandb-FGFinthecarotidarteryofratsinadosedependentmanner.ConclusionAstragalosideIVsignificantlyinhibitsneointimalhyperplasiaofratcarotidarterythroughdown-regulatingtheexpressionsofPCNAandb-FGF.

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简介：BackgroundEssentialhypertension(EH)isconsideredtobeoneofthemostimportantriskfactorofischemicstroke.Studiesaboutriskfactorsinthepatientsaremeaningfulinearlyforecastandeffectivepreventionoftheonsetofstroke.Renin-angiotensin-aldosteronesystemplaysanimportantroleintheregulationofbloodpressureandthedevelopmentofstroke,whilerecentstudieshavefoundthattheangiotensin-convertingenzyme2(ACE2)maybeareversalagentforthedevelopmentandprogressionofischemicstroke.MethodsTheACE2genewasmeasuredin139EHpatientswithischemicstrokeusingpolymerasechainreaction(PCR)andrestrictionfragmentlengthpolymorphism(PCR-RFLP)tests.Detailedandcompleteclinicalandbiochemicaldatawerecollected,includingpulsepressure,hsCRP,IMT,HDL-Canduricacidlevels.Studythecorrelationbetweenangiotensin-convertingenzyme2geneandtheriskfactorforonsetofischemicstrokeinEHpatients.ResultsPulsepressure,hsCRP,IMTanduricacidlevelshadapositivecorrelationwithon-setofischemicstrokeinEHpatients.Amongmalepatients,pulsepressure,hsCRP,IMTandHDL-CwerehigherinpatientscarryingAallelethanBallele(P<0.05).Whilethesefactorsweredifferentinfemalepa-tientscarryingdifferentgenotypesinwhichAAallelewerehighest.Patientswithvariousgenotypesshoweddifferenturicacidlevelsbutshowednosignificantdifference.ConclusionAmongEHpatientswithcomplicatedischemicstroke,thosecarryingtheA/AAalleleshowhighlevelofriskfactorsandislikelytohavethesusceptibilityofrecurrenceofstroke.

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简介：ObjectivesToinvestigatetheeffectsoftestosteroneenanthate(TE)onserumlipidsandlipoproteinsmetabolismandtheexpressionofandrogenreceptor(AR),estrogenreceptorbeta(ER-β)andplateletderivedgrowthfactorbeta(PDGFR-β)inaorticvascularsmoothmuscletissues(VSMTs).MethodsFortyagedmaleratswererandomlydividedinto4groups,groupA(placebogroup),groupB(2.5mg/kgintramuscularinjectionofTEonceaweek),groupC(5.0mg/kgintramuscularinjectionofTEonceaweek),groupD(10.0mg,/kgintramuscularinjectionofTEonceaweek).Allanimalswerefedfreelyduring16-weektreatmentperiods.TheexpressionofAR,ER-βandPDGFR-βwerestudiedbyWesternbolt.ResultsAverageserumLDL-CwasloweringroupDthanthatingroupA(p＜0.01).Comparedwiththeothergroups,averageserumTCwasalsoloweringroupD(p＜0.05).ARexpressioninaorticvascularsmoothmuscletissuescouldberegulatedbyTE:99.50±21.74,125.38±28.68and101.98±15.42forTEconcentrationsat2.5mg/kg,5.0mg/kgand10.0mg/kg,respectively,theexpressionofER-βcouldberegulatedbyTE:92.34±18.68,47.72±18.12,82.13±23.50,andtheexpressionofPDGFR-βcouldberegulatedaswellbyTE:219.70±45.59,50.16±9.72,125.36±15.74(DataforAR,ER-βandPDGFR-βproteinbandintensitywereexpressedwithx±s,withcontrolgrouptakenas100).ConclusionsThisstudyindicatesthatandrogenshavesignificanteffectsonserumlipidsandlipoproteinmetabolism.Testosteroneenanthateat5.0mg/kgcanstimulatetheexpressionofAR,butinhibitetheexpressionofPDGFR.Testosteroneenanthateattheconcentrationsof5.0mg/kgand10.0mg/kgcaninhibitetheexpressionofER-β.

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