简介:Acombinedhistopathological,mucinhistochemi-calandimmunohistochemicalstudyofthetransitionalmucosa(TM)adjacenttocolorectalcancerispresented.Twenty-sixresectedspecimenswerestudiedbyhematoxylinandeosin(HE)andhighirondiamine-alcianblue(HID-AB).Carcinoem-bryonicantigen(CEA)wasdemonstratedbyperoxi-daseantiperoxidass(PAP)technique.TheappearanceoftheTMisusuallythicker,longeranddilatedcryptswithincreasedimmatureandintermediatecells.VariableamountofsialomucinsanddecreasesulphomucinscontentaswellasincreasedCEAcontentarefoundintheTM.Thesechangesarenotseeninnon-transitionalzoneandnormalcolorectalmucosa.ItissuggestedthatthemucinchangesandexpressionofCEAintheTMmayindicateanearlyprimarypremalignantchangesandmaybeoneofthereasonsfortheTMaffectingtheprognosisofpatientswithlargebowelcancerafterradicalresection.
简介:目的:观察转移性结直肠癌病人化疗前后血清癌胚抗原(CEA)水平的变化及临床意义。方法:对施行过化疗的转移性结直肠癌病人进行回顾性研究,采用CEA试剂盒以微粒子化学发光免疫分析法测定血清CEA水平,研究CEA的变化及其与一些临床特征之间的关系。结果:67例转移性结直肠癌患者中19例(28.4%)化疗开始后70天内血清CEA有所升高,71天~210天期间再复查时,其CEA水平又降至化疗前水平或更低。影像学检查提示这19例CEA短暂升高的病人均从化疗之中得到益处(11例PR,8例sD)。没有证据支持CEA短暂升高与肿瘤原发部位、肿瘤转移、肿瘤分化之间有明确的关联。结论:某些转移性结直肠癌病人化疗开始后的血清CEA水平可以有一短暂的升高,可能与临床受益有关,尚不能作为肿瘤进展的指征。
简介:目的探讨癌胚抗原(CEA)联合神经源特异性烯醇化酶(NSE)对小细胞肺癌的诊断效能。方法选择100例小细胞肺癌患者作为研究组,100例肺部良性疾病患者作为对照组。采集患者静脉血,采用化学发光法检测血清CEA水平,采用电化学发光法检测血清NSE水平。计算CEA、NSE及CEA联合NSE诊断小细胞肺癌的敏感度、特异度。结果CEA诊断小细胞肺癌的敏感度和特异度分别为77.8%和80.2%:NSE诊断小细胞肺癌的敏感度和特异度分别为73.2%和79.7%;CEA联合NSE诊断小细胞肺癌的敏感度和特异度分别为89.2%和95.4%,均高于CEA或NSE单独诊断结果。结论CEA联合NSE对小细胞肺癌具有较高的诊断价值,可明显提高诊断敏感度和特异度,对小细胞肺癌的早期发现和治疗具有重要意义。
简介:Objective:TumorassociatedantigenencodinggeneHCA520(AF146019)wasidentifiedbyscreeningahumanhepatocellularcarcinomaexpressingcDNAlibraryusingSEREXtechnique.InthisexperimentwestudiedtheeffectofHCA520oncellproliferationandapoptosis.Methods:GeneHCA520wasgainedbyPCRandtransfectedinto293cells.ThestableexpressioncellswereobtainedbyG418selection.Thecellproliferationwasmeasuredby[3H]-TdRuptakeandapoptosisassaywasmeasuredbyFACS.Results:EukaryoticexpressionplasmidpcDNA3-HCA520wasconstructedanditsstabletransfectantswereobtained.OverexpressionofHCA520inhibitedthecellproliferationandenhancedcellapoptosisafterserumdeprivation.Conclusion:HCA520isanoveltumorassociatedantigenthatcanaffectcellproliferationandapoptosis.
简介:Objective:Ourgrouphaspreviouslyobservedthatinpatientswithsmall-celllungcancers(SCLCs),theexpressionofatumorantigen,gliomabigpotassium(gBK)ionchannel,ishigheratthetimeofdeaththanwhenthecancerisfirsttreatedbysurgicalresection.Thisstudyaimedtodeterminewhetherthisdichotomywascommoninotherpotentiallungtumorantigensbyexaminingthesamepatientsamplesusingourmoreextensiveprofileanalysisoftumor-antigenprecursorprotein(TAPP).WethentestedthehypothesisthattherapeuticinterventionmayinadvertentlycausethisincreasedgBKproduction.Methods:SCLCsamples(eightsurgicalresectionsandthreeautopsysamples)andthreecontrollungswereexaminedbyquantitativereal-timepolymerasechainreactionfor42potentialTAPPsthatrepresentpotentialT-cell-mediatedimmunologicaltargets.Results:Twenty-twoTAPPmRNAsdisplayedthesameprofileasgBK,i.e.,moremRNAswereexpressedatautopsythanintheirsurgicalcounterparts.B-cyclinandmousedoubleminute2,humanhomologofP53-bindingproteinwereelevatedinbothautopsyandsurgicalspecimensabovethenormal-lungcontrols.WhenHTB119cellswereincubatedwithdoxorubicin,gBKwasstronglyinduced,asconfirmedbyintracellularflowcytometrywithagBK-specificantibody.Conclusion:Ourfindingssuggestedthatmoreimmunologicaltargetsbecameavailableasthetumorrespondedtochemotherapyandproceededtowarditsterminalstages.
简介:Objective:Spontaneoushepatocellularcarcinoma(HCC)rupturecanbefatal,andhepaticresectioncouldachieveafavorablelong-termsurvivalamongallstrategiesoftumorrupture.However,thereisnoavailableprognosticscoringsystemforpatientswithrupturedHCCwhounderwentpartialhepatectomy.Methods:FromJanuary2005toMay2015,129patientswithspontaneousHCCruptureunderwentpartialhepatectomy.Preoperativeclinicaldatawerecollectedandanalyzed.Independentriskfactorsaffectingoverallsurvival(OS)wereusedtodevelopthenewscoringsystem.Harrell'sCstatistics,Akaikeinformationcriterion(AIC),therelativelikelihood,andtheloglikelihoodratiowerecalculatedtomeasurethehomogeneityanddiscriminatoryabilityofaprognosticsystem.Results:InthemultivariableCoxregressionanalysis,threefactors,includingtumorsize,preoperativeα-fetoproteinlevel,andalkalinephosphataselevel,werechosenforthenewtumor-associatedantigen(TAA)prognosticscoringsystem.The1-yearOSrateswere88.1%,43.2%,and30.2%forTAAscoresof0-5points(low-riskgroup),6-9points(moderate-riskgroup),and10-13points(high-riskgroup),respectively.TheTAAscoringsystemhadsuperiorhomogeneityanddiscriminatoryability(Harrell'sCstatistics,0.693vs.0.627and0.634;AIC,794.79vs.817.23and820.16;relativelikelihood,both<0.001;andloglikelihoodratio,45.21vs.22.77and21.84)thantheBarcelonaClinicLiverCancerstagingsystemandtheCanceroftheLiverItalianPrograminpredictingOS.Similarresultswerefoundwhilepredictingdisease-freesurvival(DFS).Conclusions:ThenewprognosticscoringsystemissimpleandeffectiveinpredictingbothOSandDFSofpatientswithspontaneousrupturedHCC.
简介:客观:为了与M-CSFR验证MAF-J6-1受体的抗原协会并且进一步学习M-CSF和它的受体的角色,调停了在支持白血病的房间增长的juxtacrine。方法:MAF-J6-1RRE2的Monoclonal抗体(McAb)和rhM-CSFR的polyclonal抗体(PolyAb)被准备。到M-CSFR的McAbRE2的特性被ELISA被间接ELISA,有J6-1房间殖民地形成的跨neutralizing试金和中立化测试证实。结果:到M-CSFR的净化的RE2的反应活动是超过1:16000。M-CSFR和MAF-J6-1R的禁止的活动能被RE2和anti-M-CSFR抗体堵住。到M-CSFR的RE2的反应能被M-CSFR减少。结论:到M-CSFR的RE2的特性被证实,有M-CSFR的MAF-J6-1R的抗原协会被证明。它建议M-CSF和它的受体调停了auto-juxtacrine刺激能是在白血病或nonhematological恶意的起作用的机制。
简介:Objective:Toinvestigatethetreatmentofspontaneousmetastaticlungcancerbytumorantigen-pulsed,interleukin-12(IL-12)gene-modifieddendriticcells(DC).Methods:Thespontaneousmetastaticlungcancermodel,preparedbyinjectionofthe3LLLewislungcancercellsintothefootpadsofC57BL/6mice,wastreatedbysubcutaneousvaccinationwithtumorantigenpeptidemut1-pulsed,IL-12gene-modifieddendriticcells(DC-IL-12/mut1)derivedfromthenormalbonemorrow.Aftertreatment,thelungweight,thenumberoflungmetastaticnodesandthesurvivaltimeofthetumor-bearingmicewereobserved,andtheNKandCTLactivityweredeterminedrespectively.Themiceweredividedinto8groupswith12miceineachgroup.Results:Comparedwithmicetreatedwithmut1-pulsed,controlLacZgenemodifiedDCanduntreatedDC,tumor-bearingmicetreatedwithDC-IL-12/mut1hadthelightestlungweights(P<0.01),theleastlungmetastaticnodenumber(P<0.01),thelongestsurvivaltime(P<0.01),alsowiththeinductionofpotentCTLactivity(P<0.01)andNKactivity(P<0.01).Conclusion:Tumorantigen-pulsed,IL-12gene-modifieddendriticcellshavesignificanttherapeuticeffectsonthespontaneousmetastaticlungcancer,providinganewapproachtotreatmentoflungtumors.
简介:Palliativeradiotherapy(pRT)isprimarilyemployedforpalliationofbonepaininpatientswithcastrate-resistantprostatecancer(CRPC).However,evidencethatpRTinfluencesprostate-specificantigenresponseinpatientswithCRPConsystemictherapyislacking.WedescribethreecasesofCRPCprogressingaftertreatmentwithdocetaxel(n=2)andabiraterone(n=1),whorespondedunusuallyafterpRTforbonepainwiththedevelopmentofasignificantbiochemicalresponseandrestorationofresponsetosystemictherapy.ThepossibilityofpRTinfluencingmetastaticdiseaseinCRPChasnotbeenpreviouslyreported,andraisesthepossibilityofradiation-inducedmodulationofanti-tumorimmuneresponsemechanismsthatmayplayaroleintherestorationofresponsetosystemictreatment.
简介:目的通过观察增殖细胞核抗原Ki-67在子宫内膜腺上皮的表达,探讨其在子宫内膜生理及病理学变化及意义。方法用免疫组化EnVision二步法对99例子宫内膜诊刮样本组织标记。结果14例呈分泌反应、15例萎缩、24例增生、8例不典型增生、38例子宫内膜原发癌(I型25例,II型13例)腺上皮ki-67表达率分别为0%、33.3%、83.3%,75.0%、57.9%(I型44.0%,II型84.6%)。结论ki-67与子宫内膜增殖有关,分泌反应缺失表达;Ki-67的表达与子宫内膜癌的分类有关。
简介:目的研究EBNA1和VCA-P18双多肽包被(EBNA1+VCA-P18)的ELISA检测在鼻咽癌(NPC)血清学诊断中的价值。方法用合成肽EBNA1和VCA-P18建立ELISA法,检测37例病理确诊的鼻咽癌患者血清和33例健康人血清,并与国产EBVVCA/IgAELISA试剂盒比较。结果双多肽EBNA1+VCA-P18/IgA灵敏度为91.9%,明显高于国产试剂盒的83.8%;二者特异度相同;EBNA1+VCA-P18/IgA的阳性预测值、阴性预测值、阳性似然比及优势比均高于国产试剂盒。结论双多肽EBNA1+VCA-P18/IgA相比商品化试剂盒在鼻咽癌血清学检测中有更高的灵敏度,适用于鼻咽癌早期诊断和人群筛查。