简介:Objective:Toinvestigatetheeffectofbreast-conservationtherapyinearlystagebreastcancer.Methods:Atotalof234earlystagebreastcarcinomapatientsreceivedbreastconservingtreatmentinourhospital.Aftertheoperation,theyunderwentadjuvantchemotherapyandradiotherapy.Allofthesepatientsdesiredtopreservetheirbreasts.Results:Aftermedianfollow-upof29.46months(rangefrom3to100months),3caseshadlocalrelapseand8caseshaddistantmetastasis.Theoverallsurvivalrateof5yearwas96.7%,andthediseasefreesurvivalrateof5yearwas87.85%.Conclusion:Forearlystagebreastcarcinomapatients,classicquadrantectomy,axillarydissectionandpost-operativeadjuvantchemotherapyandradiotherapyleadtoexcellentlocalcontrolandgoodsurvival.
简介:MicroRNAs(miRNAs)aresmall,non-codingsingle-strandedRNAsthatcanmodulatetargetgeneexpressionatposttranscriptionallevelandparticipateincellproliferation,differentiation,andapoptosis.Tcellshaveimportantfunctionsinacquiredimmuneresponse;miRNAsregulatethisimmuneresponsebytargetingthemRNAsofgenesinvolvedinTcelldevelopment,proliferation,differentiation,andfunction.Forinstance,miR-181familymembersfunctioninprogressionbytargetingBcl2andCD69,amongothers.MiR-17tomiR-92clustersfunctionbybindingtoCREB1,PTEN,andBim.ConsideringthatthesuppressionofTcell-mediatedimmuneresponsesagainsttumorcellsisinvolvedincancerprogression,weshouldinvestigatethemechanismbywhichmiRNAregulatesTcellstodevelopnewapproachesforcancertreatment.
简介:目的:比较592例鼻咽癌患者的CT、MRI资料,探讨MRI及CT对鼻咽癌92福州分期和02UICC/AJCC分期的不同影响。方法:分析592例治疗前进行CT/MRI检查并经病理证实为鼻咽癌的患者。比较MRI和CT检查的差异性所导致分期不同。结果:MRI分期结果较CT晚,92福州分期CT中T1+T2比例达59.4%,MRI中为39.9%(P=0.000);02UICC/AJCC分期中T1+T2比例达61.0%,MRI中为46.6%(P=0.001)。两种分期相比,92分期T4期比例为32.4%,02UICC/AJCC分期T4期比例为22.0%(P=0.001)。结论:较CT对鼻咽癌T分期的诊断具有优越性,92分期和02UICC/AJCC分期在T分期上差异不大。
简介:目的检测γδT细胞信号转导分子ζ链相关蛋白-70(ζ-chainassociatedprotein70,ZAP.70)。方法分离获取健康人PBMC,用结核杆菌低分子多肽抗原(Mtb.Ag)刺激PBMC,通过流式细胞仪检测总T细胞和丫6T细胞CD69分子的动态表达;Mtb-Ag活化俩T细胞增殖培养,10d后收集细胞,用免疫磁珠阳性分选法分离获取高纯度的γδT细胞;westernblot方法检测γδT细胞内的ZAP.70分子。结果总T细胞和γδT细胞均在活化刺激24h时表达CD69分子达高峰,但总T细胞仅为16%,γδT细胞可达75.2%;新鲜分离的PBMC中γδT细胞的比例仅为4.9%,Mtb-Ag刺激培养10d后升为69.2%,免疫磁珠阳性分选后达99-3%;检测到Y6T细胞内的ZAP-70分子。结论Mtb-Ag可特异性激活俩T细胞,用Mtb-Ag刺激γδT细胞活化增殖培养,可获得大量的γδT细胞;成功地检测到细胞内ZAP-70分子,这为Y6T细胞内其它分子的检测分析奠定方法学基础,也为进一步检测γδT细胞活化信号转导过程中ZAP-70分子的激活及作用奠定基础。
简介:Forelectronicmicroscopicobservation,wefoundSSV-transformedNIH3T3cellsweredifferentfromnon-transformedcells.InSSV-transformedNIH3T3cellsnucleicytoplasmaratiowasincreasedandincytoplasmatheribosomes(polyribosomeswereattachedtotheswollenroughendoplasmicreticulum.Itwaslikelythatribosomeswerelinedtogetherfunctionallyandstructionallytoproducespecificprotein(PDGF-likeprotein).
简介:目的分析乳腺癌3.0TMRI动态增强及扩散加权成像(DWI)的表现特征。方法回顾性分析经病理检查证实的36例乳腺癌的3.0TMRI影像资料,分析其表现。结果乳腺癌MRI表现:平扫T1WI病灶呈等或稍低信号;T2WI呈等或稍高信号;肿块形状不规则,部分呈深浅不同程度分叶状,边界模糊,并见毛刺征。DWI像:所有病灶均呈高信号,ADC图呈低信号ADC值为(0.97±0.22)×10-3mm2/s。动态增强示:不均匀强化,多呈斑点状、条片状或团状,部分病灶周围血管影增多;时间-信号强度曲线:流出型(Ⅲ型)曲线29例(80.6%);平台型(Ⅱ型)型曲线5例(13.9%);流入型(Ⅰ型)曲线2例(5.5%)。36例中34例诊断为乳腺癌,2例误诊为纤维腺瘤,诊断符合率为94.4%。结论乳腺癌具有一定MRI表现特征,结合3.0TMRI动态增强及DWI检查对乳腺癌的诊断具有较高的应用价值。
简介:Objective:ToexploretheeffectsofnuclearM-CSFontheprocessoftumorigenesis.Methods:FunctionalpartofM-CSFcDNAwasinsertedintoaneukaryoticexpressionplasmidpCMV/myc/nuc,whichcanaddthreeNLStotheC-terminaloftheexpressedproteinanddirecttheproteinintothecellnuclei.TheconstructedplasmidwastransferredintoNIH3T3cellsandthecellcloneswereselectedbyG-418selection.CellclonesstableexpressingtargetproteinwereidentifiedbyRT-PCR,ABCimmunohistochemistryassayandWesternblot.Cellgrowthkineticsanalysesthroughgrowthcurves,celldoublingtime,MTTtestandanti-senseoligodeoxynucleotide(ASODN)inhibitingcellgrowthtestwereperformedtoidentifycellsproliferationpotential.Results:Thetransfectedcellsshowedelevatedproliferationpotentialoverthecontrolcells.Conclusion:AbnormalappearanceofM-CSFinnucleuscouldenhancecellproliferation,whichsuggeststhatcytokineisoformswithincellnucleusmightplaytranscriptionfactor-likerole.
简介:Objective:ToconstructamutantpEGFP-hTERTexpressionvector,toobserveitssteadyexpressionintransfectedhumanbladdercarcinomacelllineT24anditsroleinmolecularregulatorymechanismsoftelomerase,andtoprovideanewtargetgeneforbladdercancer.Methods:PCRamplificationwasperformedbyusingprimersbasedontheknowngenesequenceofhTERT.PCRproductionwasclonedintoplasmidpGEMT-TeasyandthesequenceofmutanthTERTgenewasanalyzed.ArecombinantmutanthTERTvector(pEGFP-hTERT)wasconstructedattheEcoRIandSalIsitesofthepEGFP-C1vector.AftertransfectingthefusiongeneintobladdercarcinomacelllineT24bycalciumphosphate-DNAcoprecipitation,thesteadyexpressionofGFP-hTERTfusionproteinwastestedbyfluorescentlightmicroscopy.TheproliferationchangesofbladdercarcinomacelllineT24weredetectedbylightmicroscopyandsenescencecorrelatedβ-galactosidasestaining.Results:IdentificationofpEGFP-hTERTbyenzymedigestionshowedthatmutanthTERTfragmenthadbeenclonedintoEcoRIandSalIsitesofthepEGFP-C1vector.ThesteadyexpressionofGFP-hTERTfusionproteinwaslocalizedinthenucleusoftransfectedcells.Expressionofsenescence-associatedβ-galactosidaseintransfectedcellsgraduallyincreasedwithextendedculturedtimeandcellgrowthwassuppressed.Conclusion:Themutant-typehTERTgenesuppressestheproliferationofbladdercarcinomacelllineT24bycompetitiveeffectontelomeraseactivity.ThissuggeststhathTERTgenemightbeasuitablegenetargetforbladdercancertherapy.
简介:Objective:MemorystemTcells(Tscm)haveattractedattentionbecauseoftheirenhancedself-renewal,multipotentcapacity,andanti-tumorcapacities.However,littleisknownaboutTscminpatientswithrenalclearcellcarcinoma(RCC)andtheroleofWntsignalinginthesecells.WeevaluatedTscmfromRCCpatientsconcerningtheiractivationofWntsignalinginvitroandexploredthemechanismofpreferentialsurvival.Methods:FlowcytometryidentifiedsurfacemarkersandcytokinesproducedfromaccumulatedTscminthepresenceoftheglycogensynthasekinasebetainhibitorTWS119.Apoptosiswasevaluatedafterinductionusingtumornecrosisfactor-alpha.ImmunofluorescenceandWesternblotanalyseswereusedtoinvestigatetheactivationofthenuclearfactor-kappaB(NF-КB)pathway.Results:RCCpatientshadasimilarpercentageofCD4~+andCD8~+Tscmashealthydonors.ActivationofWntsignalingbyTWS119resultedintheaccumulationofTscminactivatedTcells,butreversalofdifferentiatedTcellstoTscmwasnotachieved.PreferentialsurvivalofTscmwasassociatedwithincreasedanti-apoptoticabilitymediateddownstreamoftheNF-КBactivationpathway.Conclusions:ThefindingthatTscmcanaccumulatebyWntsignalinginvitroinbloodfromRCCpatientswillhelpindevisingnewcancertherapystrategiesofTscm-basedadoptiveimmunotherapy,suchasdendriticcell-stimulatedTscm,andTcellreceptororchimericantigenreceptor-engineeredTscm.
简介:TheeffectofTPA,apotenttumorpromoter,onSSV-NIH3T3cellsinserum-freemediumwasinvestigated.TPAstimulatedDNAsynthesisofSSV-NIH3T3cellsonthethirddayofcultureinSFM.InSDS-PAGFofmediumconditionedbyTPA-treatedSSV-NIH3T3cells(inSFM+TPA),theamountsoffourproteinsof31.0Kd,28.5Kd,25.5Kdand13.5Kdstrikinglyincreasedoverthatofnon-TPA-treatedcounterpart(inSFM).ThePDGF-likeactivitywasalsodetectedinCMofSFM+TPA.WheninsulinandEGFweredrownofftheSFM+TPA(SFM-Ins-EGF+TPA),TPAlostitsabilitytostimulateDNAsynthesisofSSV-NIH3T3cellsonthethirddayandSDS-PAGEoftheconditionedmediumshowedthattheamountsofthefourproteinsnotedabovegratelyreduced.However,cellsinSFM-Ins-EGF+TPAwereinalmostthesamegrowthconditionascellsincompleteSFM+TPAonthethirddayofculture.Resultswerediscussedinthepaper.
简介:目的探讨恶性淋巴瘤患者T淋巴细胞亚群的变化特点。方法采用流式细胞术检测24例各型恶性淋巴瘤患者及20例正常人的外周血中T淋巴细胞(CD3+细胞)、辅助性T细胞(CD4+)、抑制性细胞毒T细胞(CD3+/CD8+)、NK细胞(CD3-/CD16+56)和NKT细胞(CD3+/CD16+56),对两者百分比进行比较分析。结果患者组中辅助性T细胞(CD3+/CIM+/CD8-)的百分比和绝对值明显低于正常对照组(P〈0.01),而患者组中CIM/CD8细胞亦明显低于正常对照组(P〈0.01)。结论在恶性淋巴瘤发生发展的过程中了解淋巴细胞亚群的变化,可为后期病情判断及可能采取的相关治疗提供依据。
简介:目的探讨X—rayrepaircross—complementinggroup1(XRCC1)RB99Q基因多态性与结直肠癌易感性的关系。方法通过计算机检索和手工检索,收集有关XRCC1R399Q基因多态性与结直肠癌易感性关系的文献,筛选出符合条件的文献,应用Meta分析软件对各项研究进行异质性检验,计算合并OR值及其95%可信区间,并行敏感性分析和发表偏倚的评估。结果国内外共有21篇文献纳入研究(结直肠癌组6229例;对照组10692例)。Meta分析结果显示:XRCC1R399Q基因多态性在整个人群中与结直肠癌无明显的关联性(ORQQvs、RR=1.10,95%CI=0.90~1.35;ORQQ/RQvs.RR=1.02,95%CI=0.90~1.16;ORQQvs.RR/RQ=1.12,95%CI=0.95~1.33)。通过种族的分层分析发现XRCC1R399Q基因多态性与结直肠癌易感性在亚洲人群和欧洲人群中无差异。结论XRCC1R399Q基因多态性与结直肠癌间不存在明显的易感性。