简介：Qian-Zheng-San,atraditionalChineseprescriptionconsistingofTyphoniiRhizoma,BombyxBatryticatus,Scorpio,hasbeenfoundtoplayanactivetherapeuticroleincentralnervoussystemdiseases.However,itisunclearwhetherQian-Zheng-Sanhastherapeuticvalueforperipheralnerveinjury.Therefore,weusedSprague-Dawleyratstoinvestigatethis.Asciaticnervecrushinjurymodelwasinducedbyclampingtherightsciaticnerve.Subsequently,ratsinthetreatmentgroupwereadministered2mLQian-Zheng-San(1.75g/mL)dailyassystemictherapyfor1,2,4,or8weeks.RatsinthecontrolgroupwerenotadministeredQian-Zheng-San.Ratsinshamgroupdidnotundergosurgeryandsystemictherapy.Footprintanalysiswasusedtoassessnervemotorfunction.Electrophysiologicalexperimentswereusedtodetectnerveconductionfunction.Immunofluorescencestainingwasusedtoassessaxoncountsandmorphologicalanalysis.Immunohistochemicalstainingwasusedtoobservemyelinregenerationofthesciaticnerveandthenumberofmotoneuronsintheanteriorhornofthespinalcord.At2and4weekspostoperatively,thesciaticnervefunctionindex,nerveconductionvelocity,thenumberofdistantregeneratedaxonsandtheaxondiameterofthesciaticnerveincreasedintheQian-Zheng-Santreatmentgroupcomparedwiththecontrolgroup.At2weekspostoperatively,nervefiberdiameter,myelinthickness,andthenumberofmotorneuronsinthelumbarspinalcordanteriorhornincreasedintheQian-Zheng-Santreatmentgroupcomparedwiththecontrolgroup.TheseresultsindicatethatQian-Zheng-Sanhasapositiveeffectonperipheralnerveregeneration.