简介：DNAmarkersenabledtodeterminethechromosomallocationsofthetwoRfgenes(Rf3andRf4)inthewild-abortivecytoplasmicmalesterility(WA-CMS)system.Foursimplesequencerepeats(SSRs)RM171,RM258,RM315andRM443wereusedtodetecttheallelicstatuswithrespecttothefertilityrestorationgenes(Rf3andRf4)in300ricecultivarsorbreedinglines.Theresultsrevealedthatoutof300lines,90linesscreenedhadRf3,65lineshadRf4,and45lineshadRf3andRf4alleles.Furthermore,45linesselectedusingSSRmarkerswerematedwithaCMSline(IR58025A)toanalyzetheirrestoringability.OffspringofallthetestlinesexceptHHZ8-SAL9DT1-Y1,HHZ5-SAL9-Y3-1andIDSA77exhibitedhigherpollenandspikeletfertility(>80%〇),thusconfirmingtheybeartheRfalleles.ThehybridoffspringofARH12-6-1-1-B-3-1,IR32307-10-3-2-1andSahel329hadthehighestpollenfertility(97.390%,98.300%and97.100%,respectively)andspikeletfertility(95.10%,97.07%and96.10%,respectively).