简介:Inthisstudy,theentiremitochondrialDNA(mtDNA)controlregion(CR)ofPholisfangiwasamplifiedviapolymerasechainreactionfollowedbydirectsequencing.ThelengthofthemtDNACRconsensussequenceofP.fangiwas853bpinlength.Inaccordancewiththerecognitionsitesaswerepreviouslyreportedinfishspecies,themtDNACRsequenceofP.fangicanbedividedinto3domains,i.e.,theextendedterminalassociatedsequence(ETAS),thecentralconservedsequenceblock(CSB),andtheCSBdomain.Inaddition,thefollowingstructureswereidentifiedinthemtDNACRsequenceofP.fangi:2ETASsintheETASdomain(TASandcTAS),6CSBsinthecentralCSBdomain(CSB-FtoCSB-A),and3CSBsintheCSBdomain(CSB-1toCSB-3).ThesedemonstratedthatthestructureofthemtDNACRofP.fangiwassubstantiallydifferentfromthoseofmostotherfishspecies.ThemtDNACRsequenceofP.fangicontainedoneconservedregionfrom656bpto815bp.Similartomostotherfishspecies,P.fangihasnotandemrepeatsequencesinitsmtDNACRsequence.PhylogeneticanalysisbasedonthecompletemtDNACRsequencesshowedthattherewerenogeneticdifferenceswithinP.fangipopulationsofthesamegeographicaloriginandbetweenP.fangipopulationsofdifferentgeographicalorigins.
简介:Mercury(Hg)isoneofthecommonlyencounteredheavymetals,whichiswidespreadininshoresedimentsofChina.InordertoinvestigatethetoxicityofHgonmarineinvertebrates,westudiedtheeffectsofthedivalentmercuricion(Hg2+)(attwofinalconcentrationsof0.0025and0.0050mgL-1,preparedwithHgCl2)onmetallothionein(MT)content,DNAintegrity(DNAstrandbreaks)andcatalase(CAT)inthegillsandhepatopancreas,antioxidantenzymeactivitiesofsuperoxidedismutase(SOD),catalase(CAT)andglutathioneperoxidase(GPx),inthehemolymph,gillsandhepatopancreasoftheportunidcrabCharybdisjaponicaforanexperimentperiodupto15d.TheresultsindicatedthatMTwassignificantlyinducedafter3d,withapositivecorrelationwithHg2+doseandtimeinthehepatopancreasandanegativecorrelationwithHg2+doseandtimeinthegills.WhileCATinthehemolymphwasnotdetected,itincreasedinthehepatopancreasduringtheentireexperiment;SODandGPxinthethreetissueswerestimulatedafter12h,bothattainedpeakvalueandthenreducedduringtheexperimentalperiod.Meanwhile,DNAstrandbreakswereallinducedsignificantlyafter12h.TheseresultssuggestedthedetoxificationstrategiesagainstHg2+inthreetissuesofC.japonica.
简介:摘要:弹性是一种能力特征,是指系统在保持基本状态不变的前提下抵御各种灾害并在灾害发生后快速恢复的能力。随着新一代信息技术的发展,产生了大量具有保存价值的电子文档。针对数字档案系统稳定发展的需要与安全风险和不可预测性之间的矛盾,研究了弹性数字档案建设的理论和方法。提高档案信息系统及其子系统在受到干扰时的跟踪、维护和快速恢复功能的能力,是实现档案数字资源的安全性、可用性和可靠性的重要基石,有利于推进网络强国和数字中国的进程。
简介:摘要:建筑行业是重要的支柱型经济产业,在经济快速增长背景下,以及城镇化建设的政策推进,建筑行业发展规模逐步提升,这同时也增加了建筑市场内部的竞争。对于建筑企业而言,为了应对市场竞争,并且在竞争中取得优势,除了“苦练外功”提高自身的技术实力之外,还需要“兼修内功”做好企业内部管理,其中材料采购管理就是需要特别关注的要点。通过对材料采购的管理,降低采购成本,这有助于扩大企业的利润空间,提升经济效益。所以,作为采购工作者来讲,就需要对材料采购管理加强研究,将供应链这一理念运用起来,融入采购管理之中,推动材料采购的优化构建,降低整体采购成本。基于此,本篇文章对基于区块链的建筑物资供应链风险因素影响评价进行研究,以供参考。
简介:摘要:在汽车总装生产中,整车输送是一个十分重要的环节,输送效率和质量在很大程度上影响生产效果。因此,需要在认识到总装输送重要性的基础上,积极引入板链输送技术,改变过去传统的输送方面,以保证输送效率,适应汽车总装及其发展提出的需求。本文针对汽车总装中的板链输送,阐述了板链输送设备的定义、分类、结构和组成,并结合实例,对其布置形式及选型设计进行深入分析,为板链输送进一步推广应用提供参考依据。
简介:摘要:电力工程的建设需要大量电力物资,电力物资的质量关系到电网的电能质量,进而与社会正常发展和人民日常生活息息相关。目前国网公司正在推广应用智慧供应链来确保电网物资的全链条管控。本文介绍了区块链技术在智慧供应链中的应用前景及其优势所在。
简介:Aciladivaricata(Hinds,1843)andA.mirabilis(AdamsandReeve,1850)arecommonbenthicbivalvesinChina.Anumberofresearchershaveproposedthatthelatterspeciesisajuniorsynonymoftheformerspecies.Becauseofmorphologicalsimilarities,itisdifficulttodistinguishthesetwospeciesbasedonvisualexaminationonly.Forbetterunderstandingoftheirtaxonomy,themitochondrialCOIgenefragmentsoffiveindividualsofA.divaricatafromtheEastChinaSeaandsixindividualsofA.mirabilisfromtheYellowSeaweresequencedinthisstudy.ThephylogeneticrelationshipsoftheobtainedCOIsequences,togetherwithnineteensequencesofthreespeciesofthegenusNucula,wereanalyzed.Thepairwiseintra-andinter-specificdistancesfortheCOIsequencesrangedfrom0.002to0.017andfrom0.128to0.134,respectively,andnooverlapwasfound.Phylogenetically,A.divaricataandA.mirabilisformdistinctcladesandclusterintoasistertoallotherNuculaspecies.TheresultsindicatedthatA.divaricataandA.mirabilisaretwodistinctspecies.Thedifferencesinthemorphologyanddistributionbetweenthetwospecieswerebrieflydiscussed.