简介:Inthisstudy,theentiremitochondrialDNA(mtDNA)controlregion(CR)ofPholisfangiwasamplifiedviapolymerasechainreactionfollowedbydirectsequencing.ThelengthofthemtDNACRconsensussequenceofP.fangiwas853bpinlength.Inaccordancewiththerecognitionsitesaswerepreviouslyreportedinfishspecies,themtDNACRsequenceofP.fangicanbedividedinto3domains,i.e.,theextendedterminalassociatedsequence(ETAS),thecentralconservedsequenceblock(CSB),andtheCSBdomain.Inaddition,thefollowingstructureswereidentifiedinthemtDNACRsequenceofP.fangi:2ETASsintheETASdomain(TASandcTAS),6CSBsinthecentralCSBdomain(CSB-FtoCSB-A),and3CSBsintheCSBdomain(CSB-1toCSB-3).ThesedemonstratedthatthestructureofthemtDNACRofP.fangiwassubstantiallydifferentfromthoseofmostotherfishspecies.ThemtDNACRsequenceofP.fangicontainedoneconservedregionfrom656bpto815bp.Similartomostotherfishspecies,P.fangihasnotandemrepeatsequencesinitsmtDNACRsequence.PhylogeneticanalysisbasedonthecompletemtDNACRsequencesshowedthattherewerenogeneticdifferenceswithinP.fangipopulationsofthesamegeographicaloriginandbetweenP.fangipopulationsofdifferentgeographicalorigins.
简介:Mercury(Hg)isoneofthecommonlyencounteredheavymetals,whichiswidespreadininshoresedimentsofChina.InordertoinvestigatethetoxicityofHgonmarineinvertebrates,westudiedtheeffectsofthedivalentmercuricion(Hg2+)(attwofinalconcentrationsof0.0025and0.0050mgL-1,preparedwithHgCl2)onmetallothionein(MT)content,DNAintegrity(DNAstrandbreaks)andcatalase(CAT)inthegillsandhepatopancreas,antioxidantenzymeactivitiesofsuperoxidedismutase(SOD),catalase(CAT)andglutathioneperoxidase(GPx),inthehemolymph,gillsandhepatopancreasoftheportunidcrabCharybdisjaponicaforanexperimentperiodupto15d.TheresultsindicatedthatMTwassignificantlyinducedafter3d,withapositivecorrelationwithHg2+doseandtimeinthehepatopancreasandanegativecorrelationwithHg2+doseandtimeinthegills.WhileCATinthehemolymphwasnotdetected,itincreasedinthehepatopancreasduringtheentireexperiment;SODandGPxinthethreetissueswerestimulatedafter12h,bothattainedpeakvalueandthenreducedduringtheexperimentalperiod.Meanwhile,DNAstrandbreakswereallinducedsignificantlyafter12h.TheseresultssuggestedthedetoxificationstrategiesagainstHg2+inthreetissuesofC.japonica.