简介:摘要; G3i仪器已经在地震勘探市场使用多年,仪器操作员积累了很多 G3i仪器的操作经验和技巧.这些实践经验和操作技巧的应用能够提高地震仪器的工作效率。本文主要探讨了 G3i仪器在地震勘探采集施工中的几个应用技巧,这些操作技巧的应用能够使得地震仪器操作变得更加高效、简单。
简介:摘要:高炉护炉一般不可避免的会造成炉况的波动甚至难行,对高炉的经济技术指标也会有不小的负面影响。唐钢 2000m高炉在维持稳定顺行可持续发展的前提下,保持了正常的经济技术指标,达到了良好的护炉效果,是对护炉优化的一次尝试。
简介:EvolutionaryevidencesuggeststhatSox3,amemberofthehigh-mobility-group(HMG)familyoftranscriptionfactors,isanancestralprecursorofSryandisinvolvedinsexdeterminationsimilartoSry.However,thereislimitedinformationregardingtheSOX3geneoftheblackrockfish(Sebastesschlegeli).Inthisstudy,wefirstisolatedSOX3genefromthegonadsofS.schlegelibyhomologycloning.Thefull-lengthofS.schlegeliSOX3(SsSOX3)cDNAwas1386bp,comprisinga906-bpopenreadingframe,whichencodesapeptideshowing93.6%and93.9%homologywiththeSox3proteinsofEpinepheluscoioidesandOryziaslatipe,respectively.ComparisonofthecDNAsequenceoftheSsSOX3genewiththecorrespondinggenomicDNAfragmentrevealedthattheSsSOX3geneconsistsofasingleexon.PhylogeneticanalysisdemonstratedtheevolutionaryrelationshipofSsSOX3withotherknownSOXB1genesinfishandtetrapods.ThepromoterregioncontainsbindingsitesofseveraltranscriptionalfactorsthatmightparticipateintheregulationofSsSOX3expression.Quantitativereal-timePCRanalysisindicatedthatSsSOX3wasexpressedinalltheinvestigatedlarvaldevelopmentalstagesfrom1to35daysafterbirthandthelevelofexpressiongraduallydecreasedasthedevelopmentproceeded.SsSOX3exhibitedsexuallydimorphicexpressioninadultgonads,withhighexpressionintheovarybutlowexpressioninthetestis.InsituhybridizationrevealedthatSsSOX3wasstronglyexpressedinoocytesandfollicularcellsofovariesbutslightlyexpressedingermcellsoftesticulartissues.Therefore,thisstudysuggeststhatSsSOX3playsanimportantroleinoogenesisandovarydifferentiationinS.schlegeli.
简介:摘要: 夏甸金矿为胶西北地区典型的蚀变岩型金矿床 , 矿体产于招平断裂带下盘的黄铁绢英岩中,主矿体产出严格受招平断裂带控制。受主断裂活动影响,在其附近形成若干次级断裂,形成Ⅶ -3号等次级矿体,呈现一定的成矿规律。
简介:摘要:随着技术深化发展, HXD3D型电力机车应运而生,具备多样化优势特征,但在运用中呈现一些问题。因此,本文多层次客观分析了 HXD3D型电力机车及其运用问题,提出了一些行之有效的改进措施,在改进、优化的基础上促使 HXD3D型电力机车在实际运用中安全、稳定以及经济运行。
简介:Inthisstudy,threecDNAsequencescorrespondingtocytochromeP450C17(CYP17I),3β-hydroxysteroiddehydrogenase(3β-HSD)andandrogenreceptor(AR)wereisolatedfromspottedseabass(Lateolabraxmaculatus).ThemRNAabundancesofCYP17Iand3β-HSDincreasedfromstageⅡtostageⅤwithasignificantincreaseatstageⅤ,andthehighestabundanceofARmRNAwasdetectedatstageⅢintesticulardevelopmentcycle.CYP17I,3β-HSDandARtranscriptswereobviouslyabundantinsteroidogenesistissuessuchastestis,brain,headkidneyamongothers.StrongandpositivesignalswereobservedmainlyininterstitialcellregionsofL.maculatustestisasweremeasuredwithinsituhybridizationmethod.SignificantincreasesofCYP17Iand3β-HSDtranscriptsweredetectedafter12–48hhCG(humanchorionicgonadotropin)andGnRHa(gonadotropin-releasinghormoneanalogue)treatments.However,anoppositerelationshipwasfoundforARintestisatthesametime.Inaddition,decreasingtrendsofCYP17Iand3β-HSDmRNAwereobservedintestisofL.maculatusinfreshwatergroup(FW)fromday2today6,andmRNAabundanceofARincreasedinbrackishwater(BW)groupfromday4today8.Thesefindingsrevealedthatthesethreesteroidsynthesisgenesareimportfortesticulardevelopment,hormoneandsalinitytreatment,andprovidedalsoaninsightintothemechanismofreproductiveendocrineofL.maculatus.