简介:Phosphatidylinositolpolyphosphates(PIPx)arerelatedwithtyrosinekinaseactivation,cellproliferationandcarcinogenesis.InordertoinvestigatetheactionmechanismofPIPx,itisdesirabletosynthesizeaffinitycolumnofPI-3,4-P2,whichisexpectedtobeabletoisolatethebindingproteinsofPI-3,4-P2.ThyraminereactedwithCH-Sepharose4Bgivingcolumn13.Thep-aminogroupof3’-(1’,2’-distearoyl-glyceryl)-1-(2-p-aminobenzyl)-3,4-di-O-phosphoryl-myo-inositylphosphate(12)wasdiazotized,thendiazo-coupledwithcolumn13togivePI-3,4-P2affinitycolumn14.ThisPI-3,4-P2affinitycolumnisaneffectivetooltopickupbindingproteinsofPI-3,4-P2.
简介:Onthebasisofapparentelasticitymethod,wederivedtheequiva-lentcircuitofcouplingvibrationofashortcolumnanddiscusseditsfrequencyequationandtheeffectsoftheloadimpedanceontheresonantfrequencyoftheshortcolumn.Bytheequivalentcircuit,wecalculatedtheinputmechanicalimpedanceofthelongitudinally-excitedshortcolumnandthecurvesoftheap-parentvibrationvelocityratioversusthegeometricaldimensionsofthecolumn.Itshowsthattheequivalentcircuitmethodisasimpleandconvenientonetoanalyzeandcalculatethecouplingvibrationoftheshortcolumn.
简介:Thedynamicbucklingofanelastic-plasticcolumnsubjectedtoaxialimpactbyarigidbodyhasbeendiscussedinthispaper.Thewholetravelingprocessofelastic-plasticwavesunderimpactactionisanalyzedwiththecharacteristicsmethod.Theregularityofstresschangesinbothcolumnendsandthefirstseparatingtimeofarigidbodyandcolumnareobtained.Byusingtheenergyprincipleandtakingintoaccountthepropagationandreflectionofstresswavesthelateraldisturbanceequationisderivedandthepowerseriessolutionisgiven.Inaddition,thecriticalbucklingconditioncanbeobtainedfromthestabilityanalysisofthesolution.Bynumericalcomputationandanalysis,therelationshipamongcriticalvelocityandimpactmass,hardeningmodulus,andbucklingtimeisgiven.
简介:喷雾器光厚度(AOT),水蒸汽内容和一个CIMEL阳光光度计在北京获得的导出的埃代表被分析。从保证质量的每日的工具,季节的趋势和内部年度的变化计算的每月的工具被介绍并且讨论。夏天在440nm有最高季节的平均AOT(τa440),埃代表(440870)并且有价值的水蒸汽内容0.93,1.34和3.0厘米分别地。第二最高季节的平均τa440与最大的变化出现在春天440870和最小4408700.99由于粗糙的粒子的影响。最小的季节的平均τa440(0.44)并且水蒸汽内容(0.4厘米)出现在冬季。年度平均τa440,440870并且水蒸汽内容为关于4年的观察,时期分别地是0.70,1.19和1.4厘米。所有每月平均的埃代表在0.8-1.4以内,它显示在北京的喷雾器是好模式、粗糙模式的粒子的很复杂的混合物(从人为的影响和自然矿物质灰尘)。
简介:Fastanalysisofthiocyanatebyion-pairchromatographyusingasilica-basedmonolithiccolumnanddirectconductivitydetectionwascarriedout.ChromatographicseparationwasperformedonaChromolithSpeedRODRP-18eusingtetrabutylammoniumhydroxide(TBA)–phthalicacid–acetonitrileaseluent.Theeffectsofeluentconcentration,eluentpHvalue,columntemperatureandflowrateonretentiontimeofthiocyanatewereinvestigated.Theoptimizedchromatographicconditionsforthedeterminationofthiocyanatewereasfollows:0.25mmol/LTBA–0.18mmol/Lphthalate–7%acetonitrile(pH5.5)aseluent,columntemperatureof308C,andflowrateof6.0mL/min.Retentiontimeofthiocyanatewaslessthan1minundertheconditions.Commonanions(Clà,NO3à,SO42àandIà)didnotinterferewiththedeterminationofthiocyanate.Detectionlimit(S/N=3)ofthiocyanatewas0.96mg/L.Calibrationgraphbetweenpeakareaandtheconcentrationofthiocyanatewaslinearintherangeof2.0–100.0mg/L.Relativestandarddeviation(RSD)ofchromatographicpeakareawas1.4%(n=5).Thismethodhasbeenappliedtothedeterminationofthiocyanateinionicliquids.Recoveriesofthiocyanateafterspikingwere100.5%.
简介:Equilibriumpathsofpost-bucklingaremeasuredforlargeslendernesscolumnspecimensmadeofthefiberreinforcedcompositematerial.Theinfluenceoftheinitialcurvatureisinvestigatedexperimentallyandcomparedwiththeresultoftheinitialpost-bucklingtheory.Boththetheoreticalandexperimentalresultsrevealthatthecolumnwiththeinitialcurvaturehasstablepost-bucklingbe-haviorsandisnotsensitivetotheimperfectionintheformofinitialcurvature.Theexperimentalre-sultsshowthatwhenthelateralbucklingdisplacementislessthan20percentofthecolumnlength,theexperimentalresultsagreewiththeresultsfromthetheoryofinitialpost-bucklingquitewell,whiletheyagreewiththeresultsfromthelargedeflectiontheoryinaquitelargerange.
简介: