简介：这个领域里的快速的发展在过去的十年眼用光技术为癌症诊断表明一个潜力。这评论加亮使用光技术的原则和优点，并且在临床的使用在肿瘤诊断和他们的限制集中于他们的申请。这些光技术是快速的方法，它能从常规方法提供很多不同信息，当时，它仍然要求临床的试用学习为临床的癌症开发并且保证这些光技术的适用性诊断。

简介：Evidence-basedpointselectionmethodsmeanselectingacupuncturepointsonthebasisofevidenceofclinicalefficacy.Whilemostofthisevidencecurrentlyderivesfromhistoricalliterature,whichrepresentstheaccumulatedclinicalexperienceofovertwomillennia,thereisalsoagrowingbodyofevidencebeingestablishedthroughacupunctureresearch.

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简介：Inthepast,filminglateralradiogramneededtheassociationoffilmingbedandanglewooden-box.Whichhasthedefectsthatitrequiredtoocomplexoperationandcan'tavoidtheeontralateralshoulderwardingofftheray.TheauthorinventedanewwaytofilmLateralradiogramofmandiblethroughyears'practice,whichismoresimpleandconvenientandsuitableforroutineoperation.

简介：Inthispaper,weestablishasurfaceelectromyography(sEMG)signalmodelandstudythesignaldecompositionmethodfromnoisybackground.Firstly,singlefiberactionpotential(SFAP),motorunitactionpotential(MUAP)andmotorunitactionpotentialtrain(MUAPT)aresimulatedbasedonthetripolarsignalsourcemodel,andthenthesEMGisobtained;secondly,thesimulatedsEMGsignalisextractedfromthemixedsignalsthatconsistsofwhitenoises,powerfrequencyinterferencesignalandelectrocardiosignalbyindependentcomponentanalysis(ICA)algorithms;lastly,thespikescorrespondingtoeachmotorunitactionpotentialfromthesimulatedsEMGsignalsweredetectedbyapplyingthewavelettransform(WT)method.SimulationresultsshowedthatsEMGmodelcoulddescribethephysiologicalprocessofsEMG,ICAandWTmethodscouldextractthesEMGsignalanditsfeatures,whichwilllayafoundationforfurtherclassifyingtheMUAP.

简介：From1990to2000,weusedHanssonpin,AOhollow-pullingscrew,andbendablescrewtotreat150casesoffreshfemoralneckfractureandtheresultwasanalysedtocomparetheefficacyofthethreemethodsofinternalfixation.

简介：客观：为了选择腰部的transpedicular的一个合适的方法，在三个方法之中在不同腰部的层次拧紧固定(Roy-Camille“s方法，Magerl”s方法和杜“smethod)在中国人口。方法：三维(3-D)图象与被电子横梁CT扫描的42个成年腰部的片断的图象数据被重建。腰部的小花梗螺丝钉固定的三个方法在3-D上被模仿重建的图象和植入小花梗螺丝钉的参数被测量。结果：在从入口点的距离有统计上重要的差别到在三个方法之间的小花梗轴(P<0.001)。Thedistances由“s方法从L1是最短的到L4，并且距离测量了byMagerl”的杜测量了s方法在L5是最短的(P<0.05)。从L1没有重要差别到L2(P>0.05)，但是对在插入TSA(横向的节角度)的安全范围的L5的从L3的重要差别在三个方法之间被发现(P<0.05)。从L3到L4，TSA的插入的安全范围由杜测量了“s”和Magerls方法比那测量byRoy-Camille显著地大“s方法(P<0.05)，但是他们之间没有重要差别(P>0.05)。AtL5，TSA的插入的安全范围由Magerl测量了“s方法在三个方法之中是最大的(P<0.05)。结论：在三个方法之中，杜“因为它到小花梗轴的从入口点的距离最短，TSA的安全范围最大，s方法是从L1toL4的最好的选择；Magerl“s方法能从L3被使用到L5并且是在L5的最好的选择；Roy-Camille”s方法在L1和L2是适用的。

简介：Hearinglossisaconditionaffectingmillionsofpeopleworldwide.Conductivehearingloss(CHL)ismainlycausedbymiddleeardiseases.ThelowfrequencyareaisthepivotalpartofspeechfrequenciesandmostfrequentlyimpairedinpatientswithCHL.AmongvarioustreatmentsofCHL,middleearsurgeryisefficienttoimprovehearing.However,variablesuccessratesandpossibleneedsforprolongedrevisionsurgerystillfrustratebothsurgeonsandpatients.Nowadays,increasingnumbersofresearchersexplorevariousmethodstomonitortheefficacyofossicularreconstructionintraoperatively,includingelectrocochleography(ECochG),auditorybrainstemresponse(ABR),auditorysteadystateresponse(ASSR),distortionproductotoacousticemissions(DPOAE),subjectivewhispertest,andopticalcoherencetomography(OCT).Here,weillustrateseveralmethodsusedclinicallybyreviewingtheliterature.

简介：Thestudyofneuronalplasticityunderpathologicalconditionsisnowamajorpointoffocusonthefieldofneurologicalrecovery.Aftertherepeatedfailureofacuteneuroprotectionstrategiesforstroketreatment,thedesignofstudiesaimedatpromotingthereconstructionofneuronalnetworkshasbecomeessential.Methodsforthedeliveryoftherapeuticagentsonasteadydosage,thuspreventingpharmacologicalpeaksorexcessivemanipulationofexperimentalanimals,arethusrequired.Additionally,methodsthatallowthevisualizationofneurologicalremodelingprocessesarefundamentaltotheunderstandingofhowatherapeuticagentexertsitsfunction.Herewedescribehowtheuseofminiosmoticpumpsforthesteadydeliveryofsuchagents,togetherwithtracttracerinjections,canbecombinedtounveilimportantinformationonhowthebrainchangesafterstrokeandhowtherapeuticagentspromotebrainremodelingrecovery.

简介：Inthemid1940s,RobertWilson(1)hypothesizedthatahighlylocalizeddepositionofenergyfromaprotonbeamcouldbeusedtoincreasetheradiationdosetotumorswhileminimizingradiationtoadjacentnormaltissues.Thedepthdosedistributionofaprotonbeamdifferssignificantlyfrom

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简介：Aim:TocomparetherecoveryrateofmorphologicallynormalandchromatincondensedspermatozoafromnativesemensamplesusingtheSpermPrepTMfiltrationcolumnsandPercollgradientcentrifugationandtodeterminetheinfluenceofthetwoprocessingtechniquesonfertilizationandpregnancyratesinanIVF-ETprogram.Methods:Sixteensemensamplesobtainedfrompatient'shusbandwereincludedinthisstudy.Eachwasdividedintotwoaliquots.ThefirstaliquotwasprocessedwithSpermPrepTMfiltrationcolumnsandthesecond,Percollgradientcentrifugation.Smearsweremadebeforeandaftersemenprocessingwithbothmethodsfortheevaluationofchromatincondensation(chromomycineCMA3)aswellasmorphology(strictcriteria)ofspermatozoa.Onehundredandseventyoocyteswereretrievedfromthepatientsandtheoocytesfromeachpatientweresubdividedintotwosets:onesetwasinseminatedusingspermatozoaprocessedwithSpermPrepTMandtheotherinseminatedaftersemenprocessingwithPercollgradientcentrifugation.Resuits:ThePercollmethodyieldedasignificantlyhigherpercentageofchromatincondensed(90.8±6.5%vs82.3±8.8%,P=0.017)andmorphologicallynormalspermatozoa(12.9±7.4%vs6.9±4.8%,P=0.001)incomparisontoSpermPrepTM.Whereas,spermcountrecoveryratewassignificantlyhigheraftertheuseofSpermPrepTMthanafterthePercollgradientcentrifugation.Thefertilizationratewassimilarbetweenthetwomethods.Conclusion:SemenprocessingwithPercollshouldberecommendedforintracytoplasmicsperminjectionasthenaturalselectionisbypassedandtheSpermPrepTMtechniquecouldberecommendedfor1VFandIUIprogramsasthespermconcentrationplaysamoresignificantroleintheseprocedures.

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简介：AbstractSingle-nucleotide polymorphisms (SNPs) are the third generation of genetic markers, having been refined from the first generation of restriction fragment length polymorphisms and the second generation of microsatellite polymorphisms. SNPs represent a focal point of current studies of Staphylococcus aureus. On one hand, this review aims to summarize common methodologies for detecting SNPs. These methods have typically included DNA genome sequencing methods and PCR-based detection methods. Alternative methods, such as mass spectrometry, denaturing high-performance liquid chromatography, SNaPshot, and SNP array have also been employed for SNP analysis. On the other hand, we enumerate a series of applications of SNP analysis in investigations of Staphylococcus aureus. SNP analysis can be applied to investigate epidemiological outbreaks and transmission of Staphylococcus aureus infections, the transmission and evolution of antimicrobial resistance genes in Staphylococcus aureus isolates, interactions of Staphylococcus aureus with other bacteria, and the links between Staphylococcus aureus in humans and livestock.

简介：AbstractBackground:The combination of high-frequency oscillations (HFOs) with single-mode imaging methods has been proved useful in identifying epileptogenic zones, whereas few studies have examined HFOs combined with multimodal imaging methods. The aim of this study was to evaluate the prognostic value of ripples, an HFO subtype with a frequency of 80 to 200 Hz is combined with multimodal imaging methods in predicting epilepsy surgery outcome.Methods:HFOs were analyzed in 21 consecutive medically refractory epilepsy patients who underwent epilepsy surgery. All patients underwent positron emission tomography (PET) and deep electrode implantation for stereo-electroencephalography (SEEG); 11 patients underwent magnetoencephalography (MEG). Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy in predicting surgical outcome were calculated for ripples combined with PET, MEG, both PET and MEG, and PET combined with MEG. Kaplan-Meier survival analyses were conducted in each group to estimate prognostic value.Results:The study included 13 men and 8 women. Accuracy for ripples, PET, and MEG alone in predicting surgical outcome was 42.9%, 42.9%, and 81.8%, respectively. Accuracy for ripples combined with PET and MEG was the highest. Resection of regions identified by ripples, MEG dipoles, and combined PET findings was significantly associated with better surgical outcome (P < 0.05).Conclusions:Intracranial electrodes are essential to detect regions which generate ripples and to remove these areas which indicate good surgical outcome for medically intractable epilepsy. With the assistance of presurgical noninvasive imaging examinations, PET and MEG, for example, the SEEG electrodes would identify epileptogenic regions more effectively.

简介：从不成熟的动物的睾丸织物的碎片种并且发展精子发生什么时候grafted到immunodeficient老鼠的下的区域上。当声言不赞成时，一样的结果被获得啮齿类动物的不成熟的睾丸被注入裸体老鼠的subcutis。那些房间重新组成生精的小管并且便于精子发生。我们比较了这二个方法，织物grafting和房间注射方法，以在immunodeficient老鼠的三紧张的背的精子发生的效率，用象施主材料的新生的猪的阴囊的纸巾和房间。裸体、严重联合immunodeficient(SCID)和NOD/Shi-SCID，IL-2Rcnull(木钉)老鼠被用作接受者。在在外科以后的10个月，transplants组织学地被检验。grafting和房间注射方法在接受者老鼠的背上导致了猪的精子发生；在transplants在场的spermatids的百分比分别地是67%和22%。用grafting方法，老鼠的所有三紧张支持了精子发生的一样的程度。至于房间注射方法，尽管SCID鼠标是为支持宪法和精子发生的最好的主机，从另外的紧张的任何差别不是重要的。当木钉老鼠没好些显示出结果，免疫不全的严厉似乎为支持xeno宫外的精子发生无关。我们的结果证实管状的宪法对猪的阴囊的房间适用。象grafting方法一样的这个方法将为在动物的不同类型学习精子发生是有用的。

简介：BACKGROUND:Withdevelopmentsoftissueengineeringandgeneticengineering,weaimtoculturemyoblasts,whicharecharacterizedbyhighpurity,highqualityandhighproduction,forwideapplicationinneuralregenerationresearches.OBJECTIVE:Tomodifytraditionaldissociationmethodinordertoobtainmyoblasts,whicharecharacterizedbyhighpurity,highqualityandhighproduction,andexplorethebiologicalpropertiesunderinvitroculture.DESIGN:Observationalstudy.SETTING:BasicInstituteofAcademyofMilitaryMedicalSciencesofChinesePLA.MATERIALS:FourneonatalWistarratsof5daysold,bothgendersandmeanbodymassof10gwereselectedinthisstudy.Themainreagentsanddevicesweredetailedasfollows:DMEMmedium(GibcoCompany),fetusbovineserum(FBS,HycolneCompany),collagenaseⅡ(SigmaCompany),trypsin(SigmaCompany),dispaseⅡ(SigmaCompany),desminantibody(FuzhouMaixinCompany),antibodyⅡandABCkit(WuhanBosterBiotechnologyCompany),deskcentrifuge(KUBATO,Japan),andinvertedphasecontrastmicroscope(LEICADMIRB,Germany).METHODS:TheexperimentwascarriedoutintheBasicInstituteofAcademyofMilitaryMedicalSciencesofChinesePLAfromJunetoOctober2006.Neonatalratsweresacrificedundersterileconditiontoobtainskeletalmusclesoflimbs,whichwerewashedwithcoldPBS(containingbenzylpenicillinandestreptomicina),andmusculartissuewasshearedintopieces.Then,thosemuscularpieceswereaddedwithmixeddigestiveenzyme(containing2g/LcollagenaseⅡ+5g/LdispaseⅡ+0.28g/LCaCl2)astwicevolumeaspieces,dealtwithmechanicalpipettingfor5minutesandculturedinCO2incubatorfor10minutes.Theoperationwasdoneforthreetimesandthemuscularpiecesweredigestedfor45minutesintotal.Moreover,cellsweresuspendedagaininordertoobtainmyoblastsfromskeletalmuscleofneonatalrats.Inaddition,myoblastswerepurifiedwithdifferentialattachmenttechniqueandenzymedigestionsoastoobservemorpho

简介：PolysaccharidesfromnumeroustraditionalChinesemedicineshavebeenprovenasthebioactiveingredientsandarehenceusedasthequalitycontrolmarkers.However,theassessmentcriteriaalwaysshowapoorspecificity,duetothelackofmaticcomparisonamongtheanalogousherbs.Inthepresentstudy,twosimilarmaterials,namelysea-tangleandsargassum,wereselectedasthemodelherbstodevelopmorespecificmethodsforqualitycontrol.Twowell-establishedmethods,determinationofthetotalpolysaccharidescontentandmonosaccharidescompositionanalysis,werebothemployed.Baseduponthequantitativeresults,theevaluationcriteriaofthepolysaccharidescontentsofnotlessthan2.0%and1.7%wereproposedforsea-tangleandsargassum,tively.NineidenticalmonosaccharidederivativesappearedontheHPLCchromatogramsofthehydrolysisandderivatizedsolutionsofthetwodrugs.Principalcomponentanalysisandorthogonalpartialleastsquaresdiscriminantanalysisusingthepeakareasofmonosaccharidesderivativesasthevariableswereperformed,andtheresultsindicatedthatmannuronicacidandxylosewiththeoppositeconcentrationsinthetwodrugswerethedifferentialcomponents.Adiscriminativecriterionusingthepeakarearatioofthesetwomonosaccharidesderivativeswasproposedforthequalitativeidentification.Inconclusion,amorespecificandquantitativequalitycontrolmethodwasdevelopedforsea-tangleandsargassum.

简介：AbstractObjective:Second-generation antipsychotics are widely used in mental illness, but the treatment effects and side effects are affected by single nucleotide polymorphisms (SNPs) of related genes. Quetiapine and aripiprazole are two frequently used secondgeneration antipsychotic drugs. The aim of this study was to develop two different SNP detection methods for four SNP alleles associated with the pharmacokinetics of quetiapine and aripiprazole, based on high-resolution melting (HRM) and multicolor melting curve assay (MMCA) respectively.Methods:Whole genome DNA samples were obtained from 240 healthy people (107 females and 133 males) without genetic diseases. HRM methods were established using four kinds of specific primers and a saturated fluorescent dye. Each SNP allele with their own primers was detected in a single reaction. In the MMCA method, a multiplex polymerase chain reaction with 4 different-colored fluorescent probes was established to detect four SNP alleles in a single reaction. All experimental protocols were approved by the Ethics Committee of the Shanghai Children’s Medical Center, China (SCMC-201015) on November 22, 2010.Results:Two detection methods for the pharmacogenomics of quetiapine and aripiprazole, based on HRM and MMCA respectively, were established in this study. The single-target HRM method can be completed in 96 minutes, whereas the quadruplex MMCA method takes 133 minutes. It was found that the results of HRM and MMCA for the four SNP alleles had 100% coincidence with Sanger sequencing in the 240 samples.Conclusion:This study developed two methods for the detection of four pharmacogenomic SNP alleles that correlated with quetiapine and aripiprazole. Both methods are rapid, cost-saving, highly accurate and potentially facilitate rational use of second-generation antipsychotics for clinical medication.

简介：ImagegeometrydistortionisanimportanttechnicalstandardinB-modeultra-sounddiagnosissystem.ThispaperinvestigatesthecauseofimagedistortioninphasedarrayB-modeultrasounddiagnosissystem.Thefirstdistortioncauseisthedelayerrorofcompoundwavebind.Phasedarrayultrasoundtranduceriscomposedofmanyrectangleunits.Withcontrollinginspiringplustime,ultrasoundwave