简介：Sincethefailureoftraditionaltherapy,genetherapyusingfunctionalDNAsequenceandsmallRNA/DNAmolecules(oligonucleotide)hasbecomeapromisingavenueforcancertreatment.ThediscoveryofRNAmoleculeshasimpelledresearcherstoinvestigatesmallregulatoryRNAfromvariousnaturalandartificialsourcesanddetermineacogenttargetforcontrollingtumorprogression.SmallregulatoryRNAsareusedfortherapeuticsilencingofoncogenesandaberrantDNArepairresponsegenes.Despitetheiradvantages,therapiesbasedonsmallRNAsexhibitlimitationsintermsofstabilityoftherapeuticdrugs,precisionbaseddeliveryintissues,precision-basedintercellularandintracellulartargeting,andtumorheterogeneity-basedresponses.Inthisstudy,wesummarizethepotentialanddrawbacksofsmallRNAsinnucleicacidtherapeuticsforcancer.

简介：

简介：AIMTo在导致的lipopolysaccharide(LPS)上调查asiatic酸(AA)的反煽动性的效果在人的角膜的上皮的房间(HCEC)的煽动性的反应.METHODSCell生存能力用数kit-8(CCK-8)的一个房间被测量试金。量的即时聚合酶链反应(qRT-PCR)被用来决定mRNAinterleukin-8(IL-8)的表示，interleukin-6(IL-6)，interleukin-1(IL-1)，肿瘤坏死factor-alpha(TNF-)，和转变生长因素--(TGF-)在HCEC。细胞内部的反应的氧种类(ROS)用ROS试金工具包被测量。谷胱甘肽(GSH)集中用全部的GSH试金工具包被测量。Akt1和Aktphosphorylation(p-Akt1)层次被西方的弄污测量，immunofluorescence.RESULTSAA在高集中导致了毒性并且显著地在20的集中刺激了HCEC的增长

简介：Ginkgolicacids(GAs),primarilyfoundintheleaves,nuts,andtestaofginkgobiloba,havebeenidentifiedwithsuspectedallergenic,genotoxicandcytotoxicproperties.However,littleinformationisavailableaboutGAstoxicityinkidneysandtheunderlyingmechanismhasnotbeenthoroughlyelucidatedsofar.InsteadofGAsextract,therenalcytotoxicityofGA(15:1),whichwasisolatedfromthetestaofGinkgobiloba,wasassessedinvitrobyusingMDCKcells.TheactionofGA(15:1)oncellviabilitywasevaluatedbytheMTTandneutralreduptakeassays.Comparedwiththecontrol,thecytotoxicityofGA(15:1)onMDCKcellsdisplayedatime-anddose-dependentmanner,suggestingthecellsmitochondriaandlysosomesweredamaged.ItwasconfirmedthatGA(15:1)resultedinthelossofcellsmitochondrialtrans-membranepotential(ΔΨm).Inpropidiumiodide(PI)staininganalysis,GA(15:1)inducedcellcyclearrestattheG0/G1andG2/Mphases,influencingontheDNAsynthesisandcellmitosis.CharacteristicsofnecroticcelldeathwereobservedinMDCKcellsattheexperimentalconditions,asaresultofDNAagarosegelelectrophoresisandmorphologicalobservationofMDCKcells.Inconclusion,thesefindingsmightprovideusefulinformationforabetterunderstandingoftheGA(15:1)inducedrenaltoxicity.

简介：AristolochiaeFructus,aChineseherbalmedicinederivedfromthefruitofAristolochiacontortaBge.,containsnephrotoxicaristolochicacidanalogues(AAAs).Accordingtoancientmedicaltexts,variousmedicinalpartsofthefruitofA.contortawereeverused.Inordertorevealwhichpartcouldbesafelyandeffectivelyused,itisnecessarytoanalyzethechemicalprofilesofdifferentmedicinalparts.HereinwecomparedthechemicalcompositionsanddeterminedaristolochicacidI(AA-I)andaristolochicacidII(AA-II)inthefourpartsviz.outerpericarp,innerpericarp,septum,andseed.Ultra-highperformanceliquidchromatographyequippedwithquadrupoletime-of-flightmassspectrometry(UHPLC-QTOF-MS)wasappliedforchemicalprofiling.Ultra-highperformanceliquidcoupledwithtriplequadrupolemassspectrometry(UHPLC-QqQ-MS)wasemployedtoquantifyAA-IandAA-IIindifferentparts.Itwasfoundthatthechemicalcompositionsofthefourpartsvariedbothqualitativelyandquantitatively.Atotalof10AAAs,including5aristolochicacidsand5aristolactams,togetherwith3alkaloids,wereunambiguouslyortentativelyidentifiedbyUHPLC-QTOF-MS.ThequantitativelyanalyticalresultsobtainedbyUHPLC-QqQ-MSshowedthatAA-IandAA-IIexclusivelyaccumulateintheseedsofA.contorta.Thesefindingsprovidesupportingdatafortherationalselectionofmedicinalparts.