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8 个结果
  • 简介:Thetitlecompoundchlorpropham(CASnumber:101-21-3,C10H12ClNO2,Mr=213.66)waspreparedbytheadditionreactionof3-chlorophenylisocyanatewithisopropanol.Spectraldata,IR,NMRandMS,werereported.ThispaperprovidessomerelatedinformationaboutRegulatoryStatus,ToxicologicalEffects,EcologicalEffectsandEnvironmentalFatealso.

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  • 简介:Thebiologytreatmentofcellulasesorhemicellulasesisusedinthispaper.Theeffectsofcellulasesandhemicellulasesonsurfacewettabilityandgluingpropertyofpoplarandwheatstrawwereinvestigated.Itwasfoundthatthesurfacecontactanglesofpoplarandwheatstrawwerereducedandthesurfacefreeenergieswereincreasedaftertreatmentbytheseenzymes.Thegluingstrengthsofpopularandwheatstrawwereenhancedwiththeincreasesofthedosageofenzymesandthetimeofenzymatictreatment.

  • 标签: 酶处理技术 可湿性 白杨 麦杆
  • 简介:有对Helicoverpaarmigera的内脏朊酶的活动的一个Bowman-Birk禁止者从Albizialebbeck的使脱去脂肪的种子面粉在0.1M钠磷酸盐缓冲区被提取。它在DEAE-SephadexA50上用铵硫酸盐降水,SephadexG-100列上的胶化过滤层析和离子交换层析与51.43%恢复被净化到29.62褶层。净化的蛋白质有由SDS页决定了的12,303daltons的分子的重量。是热被发现稳定直到60

  • 标签: 胰蛋白酶抑制剂 杀虫活性 纯化 合欢 SEPHADEX SDS-PAGE
  • 简介:在土壤和蔬菜的锑(Sb)的高集中能引起潜在的健康风险。然而,庄稼的生长和反应上的Sb的效果不是众所周知的,迄今为止,仍然为在中国的AlliticUdicFerrisols没有Sb限制标准。在这研究,一个温室实验被执行在生物资源,生理的表演,和宏上调查锑(Sb)的效果--并且绿中国卷心菜(BrassicachinensisL.)的微量元素元素集中,以及酶活动在从湖南省的AlliticUdicFerrisols,中国。锑以0的率(控制)被供应,2,5,10,20,和50mgkg−1并且因此在对待的土壤样品1.0mgkg−1,与背景珍视Sb集中是1,3,6,1121,并且51mgkg−1,分别地。结果显著地显示出卷心菜的叶生物资源和维生素酸内容(P<0.05)分别地,在30.6%和48.3%减少了并且显著地也玷污urease和脱氢酶活动(P<0.05)分别地,在33.6%和32.5%减少了什么时候玷污Sb集中,作为与控制相比是21mgkg−1。象由卷心菜的Mg,Cu,和Zn那样的必要滋养的元素的举起显然被影响,当时当土壤Sb集中超过了21mgkg−1时,可溶的糖内容稍微改变了的叶。把生理的回答和土壤酶活动基于卷心菜,为在AlliticUdicFerrisols的Sb的21mgkg−1的可允许的集中应该被推荐。

  • 标签: 脱氢酶活性 生理反应 富铁土 小白菜 中国 富铝
  • 简介:淀粉,直链淀粉,蔗糖内容和在在开花以后的shading处理下面的淀粉合成酶的活动的动态变化作为材料用二米饭变化IR72(indica)和Nipponbare(装饰用的梨树)被学习。当仅仅有点改变的自动数据处理葡萄糖pyrophosphorylase(ADPGPPase)活动,可溶性淀粉synthase活动和小粒绑了淀粉synthaseactivity时,在shading处理,thest拱门,直链淀粉和蔗糖下面,内容减少了减少,可溶性淀粉分叉酶(SSBE,Q酶)活动和小粒界限淀粉分叉酶(GBSBE,Q酶)增加的活动,和淀粉初上舞台的人ranching酶(DBE,R酶)活动与变化变化了。关联分析证明淀粉内容的变化断然并且显著地在弱光与蔗糖内容的变化被相关。ADPGPPase活动和SSBE活动断然并且显著地与淀粉累积率被相关。淀粉synthase活动的衰落与淀粉内容的减少有关,淀粉分叉酶的活动的增加在弱光下面在直链淀粉的比率的减少起了一个重要作用到全部的淀粉,这被暗示。

  • 标签: 水稻 合成酶 生物活性 淀粉
  • 简介:Effectsofshadingatthefloweringandgrainfillingstagesonosmoticregulationsubstancecontentandprotectiveenzymeactivitiesintheleavesofdifferentricegenotypes(e.g.,IIyou498,Gangyou188,Dexiang4103,Gangyou527andChuanxiang9838)wereinvestigated.TheresultsshowedthatthemalondialdehydecontentofGangyou188significantlyincreasedundershading,whereasthoseofIIyou498,Dexiang4103,Gangyou527andChuanxiang9838showednosignificantdifferencescomparedtothecontrol.ThesolublesugarcontentsignificantlydecreasedinIIyou498,Gangyou188,Dexiang4103andChuanxiang9838,whereasitdidnotsignificantlyincreaseinGangyou527undertheweaklightconditions(shading).Moreover,thesolubleproteincontentintheleavesofdifferentgenotypestendedtodecreaseundershading.Further,theprotectiveenzymeactivitiesintheleavesvariedindifferentricegenotypesundertheshadingtreatment.Basedontheosmoticregulationsubstancecontentandtheprotectiveenzymeactivitiesintheleavesofdifferentricegenotypes,itwasconcludedthatGangyou188andGangyou527hadstrongabilitiestoadapttothelowlightconditions.Inaddition,themechanismofdamagetoriceleavesindifferentgenotypesundershadingwasdiscussed.

  • 标签: 水稻基因型 保护酶活性 物质含量 渗透调节 叶片损坏 着色
  • 简介:在flowering舞台被使用二装饰用的梨树米饭变化Koshihikari和Sasanishiki在天赋和高温下面学习以后,蔗糖,fructose,蔗糖synthase的葡萄糖内容和差别迟钝,vacuolar转化酶,和细胞壁的动态变化在米饭谷物绑了转化酶。在米饭谷物,蔗糖synthase活动比转化酶的高,它显著地与淀粉累积率被相关,显示蔗糖synthase起了在蔗糖降级和淀粉合成的一个重要作用。在高温下面,没有fructose和葡萄糖的任何增加的谷物蔗糖内容的重要增加满足,建议高温处理提高了蔗糖累积,当减少时在米饭谷物的蔗糖降级。与控制植物相比,在蔗糖synthase,vacuolar转化酶,和有高温的细胞壁界限转化酶的活动的减少对待显示的植物蔗糖降级的减速与在蔗糖synthase和转化酶的活动的减少有关。

  • 标签: 高温 蔗糖合酶 空胞转化酶 细胞壁跳跃转化酶 水稻
  • 简介:Smallubiquitin-likemodifier(SUMO)-conjugatingenzymesareinvolvedinpost-translationalregulatoryprocessesineukaryotes,includingtheconjugationofSUMOpeptidestoproteinsubstrate(SUMOylation).SUMOylationplaysanimportantroleinimprovingplanttolerancetoabioticstresssuchassalt,drought,heatandcold.Herein,wereportedtheisolationofOsSCE1(LOC_Os10g39120)geneencodingaSUMO-conjugatingenzymefromrice(Oryzasativacv.Nipponbare)anditsfunctionalvalidationinresponsetodroughtstress.TheE2enzyme,OsSCE1,isoneofthreekeyenzymesinvolvedintheconjugationofSUMOtoitstargetproteins.ActivatedSUMOistransferredtothecysteineofanE2enzymeandthentothetargetlysineresidueofthesubstrate,withorwithoutthehelpofanE3SUMOligase.ExpressionofOsSCE1wasstronglyinducedbypolyethyleneglycol6000(PEG6000)treatment,whichsuggestedOsSCE1maybeinvolvedinthedroughtstressresponse.OverexpressionofOsSCE1(OsSCE1-OX)inNipponbarereducedthetolerancetodroughtstress.Conversely,thedroughttolerancewasslightlyimprovedbytheknockdownofOsSCE1(OsSCE1-KD).TheseresultswerefurthersupportedbymeasurementofprolinecontentinOsSCE1-OXandOsSCE1-KDtransgeniclinesunderinduceddroughtstress,whichshowedOsSCE1-KDtransgeniclinesaccumulatedhigherprolinecontentthanthewildtype,whereasOsSCE1-OXlinehadlowerprolinecontentthanthewildtype.ThesefindingssuggestedOsSCE1mayplayaroleasanegativeregulatorinresponsetodroughtstressinrice.

  • 标签: Oryza SATIVA drought stress small ubiquitin-like