简介:Theexperimentwascarriedouttoassessthereactionofdifferentcategoriesofricegenotypesviz.,resistant,susceptible,hybrid,scented,popularandwildinresponsetotheinfestationbyriceleaffolder(RLF),Cnaphalocrocismedinalis(Guenee)andtoexplorethepossibleuseofthesegenotypesindevelopingRLF-resistantricevarieties.Thechangesofvariousbiochemicalconstituentssuchasleafsolubleprotein,phenol,ortho-dihydroxyphenol,tanninandenzymesviz.,peroxidase,phenylalanineammonialyase(PAL)wereassessedspectrophotometricallyinallthericegenotypesbeforeandafterRLFinfestation.Theproteinprofilewasanalyzedusingsodiumdodecylsulphate-polyacrylamidegelelectrophoresis(SDS-PAGE)method.Asignificantconstituentofbiochemicalcontentsuchastannin,phenolandortho-dihydroxyphenolhasbeenincreasedalongwithenzymeactivitiesofperoxidaseandPALintheinfestedresistant(Ptb33,TKM6andLFR831311)andwildricegenotypes(OryzaminutaandO.rhizomatis).Adecreaseinleafproteincontentwasevidentinvariablyinalltheinfestedricegenotypes.Itisalsoevidentthatthecontentsofbiochemicalssuchasphenol,orthodihydroxyphenolandtanninwerenegativelycorrelatedwithleaffolderdamage.However,leafproteincontentwaspositivelycorrelatedwiththedamagebyriceleaffolder.SDS-PAGEanalysisfortotalproteinprofilingofhealthyandC.medinalis-infestedgenotypesrevealedtheenhancedexpressionofahighmolecularweight(>97kDa)proteininallthegenotypes.Besides,therewasalsoanincreasedinductionofa38kDaproteininC.medinalisinfestedresistantgenotypes,whichwasabsentinuninfestedplants.ThepresentinvestigationprovedthattheelevatedlevelsofbiochemicalsandenzymesmayplayavitalroleinriceplantsresistancetoRLF.
简介:到在米饭germplasm91-1A2的米饭胆量小蚊的抵抗被识别并且遗传上分析了。米饭人口的F1s从作为一个男父母与米饭材料Jinggui,TN1,W1263(Gm1),IET2911(Gm2),BG404-1(gm3),OB677(Gm4),ARC5984(Gm5)和Duokang1(Gm6)交叉的91-1A2被导出。到米饭胆量小蚊的所有父母线和F1,BC1F1和F2人口的抵抗被识别。结果证明91-1A2和所有F1s对中国米饭胆量小蚊遗传因子型IV抵抗。到在BC1F1和F2的易受影响的抵抗植物的分离比率被X2测试与1:3和9:7规则给予,建议到中国米饭胆量小蚊遗传因子型IV的91-1A2的抵抗被是新抵抗基因的二主导的基因控制,对已知的米饭胆量小蚊抵抗基因非突变而产生之遗传因子。
简介:Aspontaneousmutation,tentativelynamedd63,wasderivedfromthetwin-seedlingprogeniesofricecrossedbydiploidSARIIIandMinghui63.Comparedwithwild-typeplants,thed63mutantshowedmultipleabnormalphenotypes,suchasdwarfism,moretillers,smallerflagleafandreducedseed-settingrateand1000-grainweight.Inthisstudy,twoF2populationsweredevelopedbycrossingbetweend63andNipponbare,d63and93-11.Geneticanalysisindicatedthatd63wascontrolledbyasinglerecessivegene,whichwaslocatedontheshortarmofchromosome8,withinthegeneticdistanceof0.40cMfromRM22195.Hence,D63mightbeanewgeneastherearenodwarfgenesreportedontheshortarmofchromosome8.