简介:APRIL2015ChinaPledgesEducation,Hospital,JurorSystemReformsOnApr.1,China’scentralauthoritiesrolledoutplanstosupportruralteachers,overhaulpublichospitalsandimprovepublicsupervisionofcourtcases.Theplanswereadoptedatthe11thmeetingofthecentralleadinggroupfordeepeningoverallreformonWednesday,duringwhichPresidentXiJinping,whoisalso
简介:BeijinghasbeenoneoftheepicentersattackedmostseverelybytheSARS-CoV(severeacuterespiratorysyndrome-associatedcoronavirus)sincethefirstpatientwasdiagnosedinoneofthecity'shospitals.WenowreportcompletegenomesequencesoftheBJGroup,includingfourisolates(IsolatesBJ01,BJ02,BJ03,andBJ04)oftheSARS-CoV.ItisremarkablethatallmembersoftheBJGroupshareacommonhaplotype,consistingofsevenlocithatdifferentiatethegroupfromotherisolatespublishedtodate.Among42substitutionsuniquelyidentifledfromtheBJgroup,32arenon-synonymouschangesattheaminoacidlevel.Rootedphylogenetictrees,proposedonthebasisofhaplotypesandothersequencevariationsofSARS-CoVisolatesfromCanada,USA,Singapore,andChina,gaverisetodifferentparadigmsbutpositionedtheBJGroup,togetherwiththenewlydiscoveredGD01(GD-Ins29)inthesameclade,followedbytheH-UGroup(fromHongKongtoUSA)andtheH-TGroup(fromHongKongtoToronto),leavingtheSPGroup(Singapore)moredistant.ThisresultappearstosuggestapossibletransmissionpathfromGuangdongtoBeijing/HongKong,thentoothercountriesandregions.
简介:TheR(replicase)proteinistheuniquelydefinednon-structuralprotein(NSP)responsibleforRNAreplication,mutationrateorfidelity,regulationoftranscrip-tionincoronavirusesandmanyotherssRNAviruses.Basedonourcompletegenomesequencesoffourisolates(BJ01-BJ04)ofSARS-CoVfromBeijing,China,weanalyzedthestructureandpredictedfunctionsoftheRproteinincomparisonwith13otherisolatesofSARS-CoVand6othercoronaviruses.TheentireORF(open-readingframe)encodesfortwomajorenzymeactivities,RNA-dependentRNApolymerase(RdRp)andproteinaseactivities.TheRpolyproteinunder-goesacomplexproteolyticprocesstoproduce15function-relatedpeptides.Ahydrophobicdomain(HOD)andahydrophilicdomain(HID)arenewlyidentifiedwithinNSP1.ThesubstitutionrateoftheRproteinisclosetotheaverageoftheSARS-CoVgenome.ThefunctionaldomainsinallNSPsoftheRproteingivedifferentphylogeneticresultsthatsuggesttheirdifferentmutationrateunderselectivepressure.ElevenhighlyconservedregionsinRdRpandtwelvecleavagesitesby3CLP(chymotrypsin-likeprotein)havebeenidentifiedaspotentialdrugtargets.Findingssuggestthatitispossibletoobtaininformationaboutthephy-logenyofSARS-CoV,aswellaspotentialtoolsfordrugdesign,genotypinganddiagnosticsofSARS.
简介:[摘要 ] 本文用 EGS002作为核心器件,设计出了一款以直流电压为 12V的电源的车载逆变模块。主要包含 BOOST升压模块、单极控制逆变模块、辅助电源模块、滤波模块组成,分别介绍了车用逆变电源各模块的原理及其拓扑结构。根据实验测试结果显示,这款车载逆变器,可以将输入的低压直流电转换为电气设备需要的工频交流电输出,一般是 220V、 50Hz的交流电压。本模块设计的转化的效率高达 95%甚至更高,输出电压的波形比较稳定,总谐波失真较小,工作性能牢靠。此外,本次设计的车载逆变模块还能够防止过压、防止过流、防止过热等问题。