简介:AIM:Toexploretheeffectsandmechanismofvascularendothelialcadherin(VE-cadherin)onexperimentalcornealneovascularization(CRNV).·METHODS:MousecorneaswereburnedwithsodiumhydroxidetobuildaCRNVmodel.Theburnedcorneaswerelocallyadministratedwithanti-mouseVE-cadherinneutralizingantibody.AnnexinVandclusterofdifferentiation31(CD31)doublestainingwasusedtomeasurevascularendothelialcellapoptosiswiththeuseofflowcytometry(FCM).TheproteinexpressionofNADPHoxidase2(Nox2),caspase-3,andproteinkinaseC(PKC)intheburnedcorneaswereexaminedbyWesternblot.Humanretinalendothelialcell(HREC)proliferationwasdetectedusingaCellCountingKit8(CCK-8)assayinvitro.·RESULTS:TheamountofCRNVpeakedtwoweeksafterthealkaliburn.FCMconfirmedthatVE-cadherinneutralizingantibodytreatmentincreasedCD31positivecellapoptosis.WesternblotrevealedthattheintracornealproteinexpressionofNox2andcaspase-3wereup-regulated,whilePKCwasdown-regulatedintheVE-cadherinneutralizingantibodyadministratedgroup.CCK-8assayshowedthatVE-cadherinneutralizingantibodymarkedlyinhibitedHRECproliferation.·CONCLUSION:VE-cadherinexhibitedananti-apoptosiseffectthroughenhancedPKCsignalingandanenhancedcellproliferationpathway.
简介:读完这期杂志,你记住了多少知识呢?请找出下面几道题的答案,并把答案邮寄回编辑部(也可以发送邮件到hlj1951t@sina.com),全部答对的同学可以参加抽奖活动,赢取精美礼品。1.2015年9月3日,纪念中国人民抗日战争暨世界反法西斯战争胜利()周年大阅兵在北京天安门广场隆重举行。A50B60C70D802.被称为'地球历史上最大的飞行动物'的是()。
简介:目的探讨VE—cadherin和hs—CRP在心绞痛和心肌梗死患者中的检测意义。方法选择收治的心绞痛和心肌梗死患者共90例。其中60例为心绞痛组,根据类型又分为稳定型心绞痛组30例和不稳定型心绞痛组30例,其余30例为心肌梗死患者,作为心肌梗死组。同时选择健康体检者30例,作为对照组。检测上述对象的VE—cadherin和hs—CRP水平。结果心肌梗死组和心绞痛组的VE—cadherin和hs—CRP水平均高于对照组,差异有统计学意义(P〈0.05);心肌梗死组的VE—cadherin和hs—CRP水平高于心绞痛组,差异有统计学意义(P〈0.05)。心肌梗死组的VE—cadherin和hs—CRP水平均高于稳定型心绞痛组和不稳定型心绞痛组,差异有统计学意义(P〈0.05);不稳定型心绞痛组的VE—eadherin和hs—CRP水平高于稳定型心绞痛组,差异有统计学意义(P〈0.05)。结论VE—cadherin和hs—CRP联合检测有助于监测冠心病患者的病情,具有重要临床检测意义。
简介:摘要目的探讨PTEN和E-cadherin在乳腺癌组织中表达与临床各病理特征的关系.方法采用免疫组化S-P法分别对72例乳腺癌组织中PTEN和E-cadherin蛋白的表达水平进行检测,结合临床病理资料进行统计学分析.结果(1)PTEN低表达与组织学分级、腋窝淋巴结转移、ER阴性表达密切相关(P<0.05).(2)E-cadherin在乳腺癌组织中表达与乳腺癌组织学分级、ER、HER-2有关(P<0.05).(3)经Spearman相关性检验分析示PTEN表达与E-cadherin表达呈正相关(r=-0.245,p<0.05).结论(1)PTEN蛋白的表达缺失可能在ER阴性乳腺癌特别是basal-like(三阴)型乳腺癌中的发生、发展有重要作用.(2)E-cadherin可能是乳腺癌一个独立的预后因素.关键词乳腺癌,PTEN,E-cadherin,免疫组化,分子分型ExpressionandclinicalsignificanceofPTENandE-cadherininthemolecularsubtypesbreastcancerANWen,YUNLi-feng.DepartmentofOncologySurgery,CenGtralHospitalofUlanqab,Ulanqab012000,chinaAbstractObjectiveTodiscussPTENandE-cadherininbreastcancertissuestherelationshipbetweentheexpressionandclinicalpathologicalfeatures;.MethodsBySPimmunohistochemicalmethodtodetecttheexpressionlevelsofPTENandE-cadherinin72casesofbreastcancer,combinedwithclinicalandpathologicalfeatureswereanalyzed.Results(1)PTENlowexpressionwithhistologicalgrade,axillarylymphnodemetastasisandtheexpressionofER-negative(P<0.05).(2)E-cadherinexpressionandhistologicgrade,ER,HER-2related(P<0.05).(3)TheSpearmancorrelationtestanalysisfoundthatPTENexpressionofEcadherinexpressionwaspositivelycorrelated(r=-0.245,p<0.05).Conclusion(1)LossofexpressionofPTENproteinmayespeciallybasal-like(triple-negative)breastcancerintheoccurrenceanddevelopmenthasanimportantroleinER-negativebreastcancer.(2)E-cadherinmaybeanindependentprognosticfactorinbreastcancer.KeywordsBreastcancer,PTEN,E-cadherin,immunohistochemistry,molecularsubtypes中图分类号R737.9文献标识码B文章编号1008-6315(2015)12-0265-02
简介:摘要目的分析弥漫大B细胞淋巴瘤与淋巴结反应性增生患者淋巴结组织中T-cadherin、MRP1阳性表达水平,以及与临床预后相关指标之间的关系。方法通过免疫组化法检测42例DLBCL(弥漫大B细胞淋巴瘤)和24例淋巴结反应性增生组织中T-cadherin、MRP1的表达水平,以及与临床预后相关指标(年龄、性别、WBC、PLT、HB、临床分期、结外累及部位和近期疗效)间的关系。分析T-cadherin、MRP1的表达水平与DLBCL发病及侵袭进展的相关性。结果T-cadherin在弥漫大B细胞淋巴瘤中表达水平显著低于淋巴结反应性增生,T-cadherin表达水平与弥漫大B细胞淋巴瘤患者年龄、性别、WBC、PLT、HB无相关(均P>0.05),与患者临床分期、结外累及部位和近期疗效有关(均P<0.05)。MRP1表达水平与弥漫大B细胞淋巴瘤患者年龄、性别、WBC、PLT、HB、无相关(均P>0.05),与患者临床分期、结外累及部位和近期疗效有关(均P<0.05)。MRP1在弥漫大B细胞淋巴瘤中表达水平显著高于于淋巴结反应性增生。结论弥漫大B细胞淋巴瘤患者中T-cadherin的缺乏影响发病及预后,T-cadherin的缺乏可能与多药耐药相关。