简介:目的总结cD4T细胞低于150个·μL-1晚期艾滋病的临床特征,提高对晚期艾滋病患者各种机会性感染及非特异性并发症的认识。方法回顾性分析2004年8月至2010年4月收治的30例CD4T细胞低于150个·μL-1艾滋病患者的临床资料。结果此类艾滋病患者以无业吸毒人员居多,高效抗反转录病毒治疗(HAART)治疗依从性多数较差。临床表现具有多样性,除了发热、咳嗽、消瘦等常见症状外,还表现为多部位、多种机会感染并存,贫血、肝。肾功能损害、严重营养不良等非特异性并发症普遍存在,达27例。结论CD4叮细胞低于150个·μL-1晚期艾滋病患者病情复杂多样。普遍较危重,治疗困难,预后一般极差。
简介:目的观察高血压病人奥美沙坦治疗前、后血清hs—CRP、ICAM-1、sCD40L的浓度变化,探讨奥美沙坦在有效降压的同时是否具有抗炎作用。方法选择我院门诊新发现或未经长期降压治疗的轻、中度高血压病人53例(男41例,女12例),均符合WHO/ISA1999年的诊断标准,平均年龄(53.23±7.60)岁,所有人选者抽取空腹12h静脉血,用ELISA方法测定血清hs—CRP、ICAM-1、sCD40L的水平,均口服奥美沙坦20mg/d,4周后若舒张压〉90mmHg,剂量加倍40mg/d,共服药8周,8周后抽血。所有入选者均测血压、血脂、血糖,服药8周后重复测定以上所有指标。结果(1)高血压病人用药前、后血脂、血糖均无差异(P〉0.05),具有可比性;治疗后血压有明显下降(P〈0.01);(2)高血压用药后hs—CRP、ICAM-1、sCD40L明显下降(分别为1.66±1.34mg/Lversus2.37±1.81mg/L,P〈0.01;152.25±50.72ng/mlversus204.86±49.33ng/ml,P〈0.01;3298.50±1309.01pg/mlversus5191.72±1144.63pg/ml,P〈0.01)。结论奥美沙坦在有效降压的同时具有抗炎作用。
简介:睁开双眼,我看到的是摇曳在空中那被风吹动的树叶。细细的呼啸声与耳膜产生共鸣,使我不禁感到一丝凉意。天气阴沉,应该是快要下雨了吧。我甩了甩脑袋,试图使自己的头脑清醒一下。长时间躺在这公园的长椅上使我感到腰酸背疼,是该起身活动活动筋骨了啊。
简介:InthispaperitturnsoutthataBanachlatticeXisorderisomorphictol~1(Γ)forsomenonemptysetΓiffitisaSchurspaceandalltheinfinitelydimensional,separableandclosedidealsofXareorderisomorphic.
简介:Letf(x)∈C[-1,1],pn*(x)bethebestapproximationpolynomialofdegreentof(x).G.Iorentzconjecturedthatifforalln,p2n*(x)=p2n+1*(x),thenfiseven;andifp2n+1*(x)=p2n+2*(x),po*(z)=0,thenfisodd.Inthispaper,itisprovedthat,undertheL1-norm,theLorentzconjectureisvalidconditionally,i.e.if(i)(1-x2)f(x)canbeextendedtoanabsolutelyconvergentTehebyshevsories;(ii)foreveryn,f(x)-p2n+1*(x)hasexactly2n+2zeros(or,inthearcondsituation,f(x)-p2n+2*(x)hasexaetly2n+3zeros),thenLorentzconjectureisvalid.
简介:建立相对论性的组分夸克模型,求解了B和(?)、(?)的BS波函数,并计算出B→(?)和B→(?)跃迁强子矩阵元的形状因子及其斜率,进而计算出半轻子弱衰变B→(?)l+vt和B→(?)l+vt的衰变宽度.与实验值比较定出CKM矩阵元│vcb│=0.042±0.003.
简介:identification and classification of learning strategy. The problems are reviewed concerning the definition and classification of learning strategies and then the paper tentatively introduces Cohen’s approach to defining learning strategies in terms of prototypicality of features of learning strategies.,Cohen’s attempt to describe the prototypicality of strategies is a step forward concerning defining learning strategies. It might reflect the nature of learning strategies to a large extent because the answers to the questionnaire come from strategy experts. It is true that some problems still exist. For example,all-or-nothing feature but in terms of how far along a continuum a feature could possibly go before it stopped being descriptive of a strategy. This is an approach of defining learning strategies in terms of how prototypical the feature was. These features as follows are arranged in a descending order of agreement
简介:Radialfunctionshavebecomeausefultoolinnumericalmathematics.Onthespheretheyhavetobeidentifiedwiththezonalfunctions.Weinvestigatezonalpolynomialswithmassconcentrationatthepole,inthesenseoftheirL1-normisattainingtheminimumvalue.Suchpolynomialssatisfyacomplicatedsystemofnonlineare-quations(algebraicifthespacedimensionisodd,only)andalsoasingulardifferentialequationofthirdorder.Theexactorderofdecayoftheminimumvaluewithrespecttothepolynomialdegreeisdetermined.Byourresultswecanprovethatsomenodalsystemsonthesphere,whicharedefinedbyaminimum-property,areprovidingfundamentalmatriceswhicharediagonal-dominantorboundedwithrespecttothe∞-norm,atleast,asthepolynomialdegreetendstoinfinity.
简介:摘要目的构建肿瘤相关抗原表皮生长因子受体特异性嵌合抗原受体(EGFR-CAR)和程序性死亡受体-配体1(PD-L1)抗体双修饰慢病毒载体表达系统。方法人PD-L1-Fc蛋白免疫BALB/c小鼠,经细胞融合、亚克隆筛选高分泌PD-L1特异性抗体的稳定杂交瘤,酶联免疫吸附试验(ELISA)和Western blot检测抗体特异性,流式细胞术(FACS)鉴定对PD-1配受体封阻性能,Fortebio测定抗体亲和力,抗体全长测序,经保留鼠源CRD1、CRD2和CRD3人源化改造后构建单链抗体(single-chain variable fragment,scFv);人EGFR单克隆抗体杂交瘤系,经5′RACE技术扩增其轻链和重链可变区(VL和VH)基因,构建scFv,克隆至真核载体pcDNA3.1表达鉴定。基因合成EGFR-CAR(引入CD137协同信号胞内功能域)与PD-L1-scFv借助2A序列连接,克隆入慢病毒pLVX-EF1a-IRES-ZsGreen1表达载体,使用Lenti-X Packaging Single Shots (VSV-G)共同转染293T细胞,获得包装病毒,感染293V细胞,FACS测定CAR膜表达,ELISA检测CAR感染293V细胞培养上清中PD-L1-scFv表达情况,转染激活人外周血T细胞,验证CAR膜表达。结果获得PD-L1抗体11E3,具备高度配受体封阻性能,经人源化改造后,亲和力稳定(2.67×10-10 mol/L),EGFR-scFv获得有效表达。进一步构建了EGFR-CAR和PD-L1双修饰慢病毒分泌型CAR(CTC0537-1)及膜表达型CAR(CTC0537-2),其病毒感染293V细胞阳性率为10%。CTZ0431-1感染293V细胞后,细胞膜表面表达EGFR-scFv,检测培养上清存在PD-L1-ScFv;CTZ0431-2感染293V细胞后,细胞膜表面EGFR-scFv和PD-L1-scFv有效表达,双表达病毒感染活化T细胞的CAR表达率为39.3%。结论成功构建了EGFR-CAR和PD-L1-scFv双表达慢病毒载体,EGFR-CAR中度结合亲和力,此为EGFR靶向和PD-L1抗体双修饰CAR-T细胞的实体瘤治疗研究提供了关键工具。