简介：AbstractWe analyzed variations in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome during a flight-related cluster outbreak of coronavirus disease 2019 (COVID-19) in Shenzhen, China, to explore the characteristics of SARS-CoV-2 transmission and intra-host single nucleotide variations (iSNVs) in a confined space. Thirty-three patients with COVID-19 were sampled, and 14 were resampled 3-31 days later. All 47 nasopharyngeal swabs were deep-sequenced. iSNVs and similarities in the consensus genome sequence were analyzed. Three SARS-CoV-2 variants of concern, Delta (n = 31), Beta (n = 1), and C.1.2 (n = 1), were detected among the 33 patients. The viral genome sequences from 30 Delta-positive patients had similar SNVs; 14 of these patients provided two successive samples. Overall, the 47 sequenced genomes contained 164 iSNVs. Of the 14 paired (successive) samples, the second samples (T2) contained more iSNVs (median: 3; 95% confidence interval [95% CI]: 2.77-10.22) than did the first samples (T1; median: 2; 95% CI: 1.63-3.74; Wilcoxon test, P = 0.021). 38 iSNVs were detected in T1 samples, and only seven were also detectable in T2 samples. Notably, T2 samples from two of the 14 paired samples had additional mutations than the T1 samples. The iSNVs of the SARS-CoV-2 genome exhibited rapid dynamic changes during a flight-related cluster outbreak event. Intra-host diversity increased gradually with time, and new site mutations occurred in vivo without a population transmission bottleneck. Therefore, we could not determine the generational relationship from the mutation site changes alone.

简介：[摘要]目的：本研究通过回顾性分析2022年3月至2022年5月，感染新冠病毒，入住“长春通源方舱医院”的患者。剔除重复登记和治愈后再次复阳入院患者。总结归纳病人的个人情况等因素，分析患病情况，为未来疫情防控及传染病防控提供借鉴。结论：1.感染患者年龄阶段与人口普查比较，无明显差异。2.性别构成与人口基数相比男性患者感染多于女性。3.传播途径统计不明原因感染最多，明确原因患者也以家人邻居传染为主。4.职业特点分析感染者以无业退休人员和打工创业人员为感染最多人群。5.症状分析以咳嗽为首要症状，其次为无症状患者居多。6.疫苗接种情况，接种2针患者感染最多，其次为3针患者。7.基础疾病分析，多数为无明确基础疾病患者，有基础疾病人员以高血压病，心脏病和糖尿病居于前3位。