简介：AgreatamountoffoodbornepathogenswereGram-positive(G?)bacteria,athreattopublichealth.Inthisstudy,consideringthebindingabilityofnisintowardsG-bacteriaandthestablefluorescentabilityofEGFPprotein,afluorescentnisin–EGFPproteinprobewasconstructedbyageneengineeringmethod.NisinandEGFPwereusedasthereceptorandfluorophore,respectively,todetectG?bacteria.ThenisinandegfpgenewereamplifiedseparatelyaccordingtothesequencepublishedinGenBankusinguniqueprimers.ThetwogeneswereclonedintoapET-28b(?)vectorresultinginapET-28b(?)–nisin–egfpvector.ThevectorwastransferredintoEscherichiacoli(E.coli)BL21(DE3)forexpression.Theexpressedproteinwasextracted,purifiedbyaNi–NTAcolumn,andthentestedbytheSDS-PAGEmethodtoconfirmitsmolecularweight.Listeriamonocytogenes(L.monocytogenes),Staphylococcusaureus(S.aureus),andMicrococcusluteus(M.luteus)wereusedastherepresentationsofG?bacteria.E.coliO157,representingthegram-negative(G-)bacteria,wasusedasanegativecontrol.Thebindingspecificityoftherecombinantproteinwasperformedontwotypesofbacteriaandthendetectedthroughfluorescentmicroscopy.Theresultsindicatedthatthenisin–EGFPprobecoulddetectG?bacteriaat108CFU/mL.

简介：Itisnecessarytounderstandthebacterialpopulationsassociatedwithricesoastoprovidemoreinformationandnaturalresourcesforeffectivemanagementofmajordiseasesinrice.Asurveyonscreeningandidentificationofgram-positivebacteriawasconductedduring1998-2004.Sevenhundredandfifty-sixricesampleswerecollectedfromZhejiang,Jiangsu,FujianandYunnanProvinces,China.Over1000bacterialisolateswereisolatedandtestedforcolonymorphology,pathogenicity,andsomecharacteristicsofbacteriologyincludingGramstaining,fluorescentpigmentonKingsmediumBandmicroscopicobservationforendospore.Togetherwithfivestandardreferencestrains,74representativegram-positivebacterialisolateswereconfirmedbyBiologandgaschromatographicanalysisoffattyacidmethylesters.FivebacterialspeciesofBacillusandotherthreegenerawereidentifiedandisolatesfromBacillussublitisandBacillusmegaterium,exhibitedthemosteffectiveinhibitionagainstthepathogensofsheathblightandbakanaediseaseofrice.AfewisolatesfromBacilluspumilusandBacillusmegateriumshowedweakvirulentonricetogetherwithsomevirulentisolates,dskshouldbeconsideredwhenisolatesfromthesespecieswerescreenedforbiocontrolagents.

简介：BackgroundSepticshockcausedbybacteremiaisalife-threateninginfectionwhoseprognosisishighlydependentonearlyrecognitionandappropriatetreatment.Procalcitonin(PCT)hasbeenshowntoaccuratelyandquicklydistinguishbacteremiafromnoninfectiousinflammatorystatesincriticallyseverepatients.However,theextentofPCTmagnitudeelevationaccordingtotheGramstainresultinelderlypatientswithcoronaryheartdisease(CHD)attheonsetofsepticshockcausedbybacteremiavaries,andhasnotbeenclearlyelucidated.MethodsThemedicalrecordsofadvancedage(non-neutropenic)patientwithCHDandsepticshockbetweenMar2013andJun2015whohadbacteremiacausedbyeitherGram-positive(GP)bacteriaorGram-negative(GN)bacteriawerereviewed,andthelevelsofPCT,C-reactive(CRP)proteinandwhitebloodcellscount(WBC)inbothgroupswereanalyzed.Results75episodesofeitherGNbacteremia(n=40)orGPbacteremia(n=35)wereenrolled.PCTlevelswerefoundtobemarkedlyhigherinpatientswithGNbacteremiathaninthosewithGPbacteremia[(8.93±17.58)vs.(64.42±58.56)ng/L(P<0.001)],whereastherewasnosignificantdifferenceinCRPandWBC(P>0.05).Moreover,ahighPCTlevelwasfoundtobeindependentlyassociatedwithGNbacteremiainthisstudypopulation.APCTlevelof19.69ng/mLyieldeda72.5%sensitivity,a91.4%specificity,an8.43positivelikelihoodratioanda0.30negativelikelihoodratioforGN-relatedbacteremiainthestudycohort[AUROCC=0.870(0.041),95%CI(0.790-0.949)].ConclusionInanelderlypatient(non-neutropenic)withCHDandsepticshock,GNbacteremiacouldbeassociatedwithhigherPCTvaluesthanthosefoundinGPbacteremia(PCT>19.69ng/mL).

简介：正面思考是什么呢?正面思考意味着只去想好的事情、对事情成功有益的因素，而不考虑任何会引起事情没有价值、无法实施、导致失败的因素。

简介：Thecompletelypositivematricesareimportantinthestudyofblockdesignsarizingincombinatorialanalysis(see[11])andarerelatedtocopositivematrices,whichhaveappli-cationsincontroltheoryandinmultimobicalprogramming(see[12]).Ann×nmatrixAissaidtobecompletelypositiveifAcanbefactoredasA=BB~Tforsomen×mrealmatrixBwithnonnegativeentriesforsomem<∞.IfAisacompletelypositivematrix,thenthesmallestnumberofcolumnsinanonnegativematrixBsuchthat

简介：Thispaperinvestigatesthepositiverealcontrolproblemforuncertaindescriptorsystems.Theparametricuncertaintyisassumedtobenormbounded.Firstly,forthenominalsystem,anewpositiverealcharacterizationisgiven,whichisexpressedbyastrictlinearmatrixinequality(LMI)withoutequalityconstraints.Secondly,fortheuncertainsystem,necessaryandsufficientconditionsforthesolvabilityofthepositiverealcontrolproblemarederived.Basedontheseconditioosastatefeedbacklawisobtained,whichrenderstheresultantclosed-loopsystemrobustlypositivereal.

简介：本文使用N元语言模型，针对维吾尔语详细词典中的维吾尔语单词进行字母构成统计分析。使用Morogram工具，以字母为主要分析单位，对维吾尔语单词的构词特性进行研究。本文研究成果可以进一步提高我们对维吾尔语构词方法的理解，在语言学、机器翻译学、词典构建等方面具有重要的应用价值。

简介：Toexploretheprevalenceoftheplasmid-mediatedquinoloneresistancegeneqnrAinGram-negativebacteriaandtoinvestigateitsmoleculargeneticbackgroundandresistanceprofileinisolatesharboringthisgene,atotalof629nalidixicacid-resistantisolatesofnon-repetitiveGram-negativebac-teriawerecollectedfromclinicalspecimensbetweenApril2004andApril2006andtheseisolateswerescreenedforqnrAgenebyPCRusingspecificprimerscombinedwithDNAsequencing.Theextendedspectrumβ-lactamase(ESBL)orAmpC-producingisolatesweredistinguishedbythephenotypicconfir-matorytestcombinedwithDNAsequencing,andtheantibioticssusceptibilitytestforqnrA-positiveiso-lateswascarriedoutbyKirby-BauerandE-testmethod.TodetectthelocationoftheqnrAgene,plas-midconjugationandSouthernhybridizationwereperformedandtheintegronstructurecontainingtheqnrAgenewasclonedbyPCRstrategyandsequencedbyprimerwalking.ItwasdemonstratedthattheincidenceoftheqnrA-positivestrainsinnalidixicacid-resistantbacteriawas1.9%(12/629),inwhichthedetectionratesforKlebiesiellapneumoniae.Enterobactercloacae,Enterobacteraerogenes,CitrobacterfreundiiandSalmonellachoeraesuiswere2.2%(3/138),17.1%(6/35),9.1%(1/11),12.5%(1/8),and14.3%(1/7),respectively.TheqnrAgenewasfoundtobeembeddedinthecomplexsu/1-typeintegronlocatedonplasmidswithvariedsize(80-180kb).Amongthem,4qnrA-positiveisolatescarriedintegronIn37and8isolatescarriedanovelintegron,temporarilydesig-natedasInX.AlltheqnrA-positiveisolateswereESBL-producingandtransferableforthemuhi-drugresistance.Itisconcludedthattheplasmid-mediateddrug-resistancemechanismexistsinthequinoloneresistantstrainsofisolatesfromhospitalsinGuangdongarea,buttheincidencewasratherlow.Never-theless,itisstillpossiblethatthehorizontaltransferoftheresistantqnrAgenemightleadtothespreadingofdrug-resistance.

简介：纸为积极Markovian涉及积极观察员设计跳有部分已知的转变率的系统。由使用一个线性co积极的类型Lyapunov-Krasovskii函数，一个足够的条件被建议保证错误的随机的稳定性积极系统和积极观察员的存在，它在线性编程被计算。最后，一个例子被给表明主要结果的有效性。

简介：为任意的给定的积极条款系列$\sum\limits_{k=1}^\infty{a_k}$并且$\sum\limits_{k=1}^\infty{b_k}$，现在的纸为$\mathop提供一个有趣的必要、足够的条件{啜}\limits_{n\geqslant1}\{\sum\limits_{k=1}^n{a_k/\sum\limits_{k=1}^n{b_k}}\}=\infty$。

简介：LetAbeann×nrealsymmetricmatrix.Aiscalledcompletelypositive(denoteA∈CP_n)ifA=B’Bforsomem×nnonnegativematrixBwheremisanintegeranddenotes

简介：在这个工作，我们在一半线上涉及单个边界价值问题的积极解决方案的存在和复合。从积极一半线上的传染病学和燃烧理论集合的二个问题被调查。我们使用在适当Banach空格在锥上与固定的点索引相结合的上面、更低的解决方案技术。结果在文学补充最近的。

简介：

简介：Byconsideringthepropertiesoff(t,u,v)/u+v,g(t,u,v)/u+v,weshovthemultiplicityofatleasttwopositivesolutionsoftheellipticsystem.

简介：最近，在矩阵是积极semidefinite和入口明智的nonnegative的地方，研究人员们对学习semidefinite编程(SDP)松驰模型感兴趣，为二次地抑制的二次的编程(QCQP)。比作基本SDP松驰，这个二倍地积极的SDP模型拥有另外的O(n2)限制，它与O(n)限制为基本模型使SDP答案复杂性比那实质地高。在这份报纸，我们证明二倍地积极的SDP模型与一套有效二次的切割等价于基本的。当QCQP对称、同类时(它代表许多古典组合并且nonconvex优化问题)，甚至没有任何有效切割，二倍地积极的SDP模型等价于基本SDP。在另一方面，二倍地积极的SDP模型能帮助紧缩界限直到36%，但是不再。最后，我们设法把一些以前的结果递四次的模型。

简介：Inordertostudytheapproximationbyreciprocalsofpolynomialswithrealcoefficients,onealwaysassumesthattheapproximatedfunctionhasafixedsignonthegiveninterval.Sometimes,theapproximatedfunctionispermittedtohavefinitesignchanges,suchasl(l≥1)times.ZhouSongpinghasstudiedthecasel=1andl≥2inLpspacesinorderofpriority.Inthispaper,westudiedthecasel≥2inOrliczspacesbyusingthefunctionextend,modifiedJacksonkernel,Hardy-Littlewoodmaximalfunction,Cauchy-Schwarzinequality,andobtainedtheJacksontypeestimation.

简介：批评话语分析(简称CDA)以社会问题尤其是诸如性别、种族、阶级、年龄歧视等种种不平等的现象为针砭的对象,通过解剖大量而具体的真实语料,以客观的语言证据和详尽的分析,把社会冲突归因于权力,把批评的矛头指向政府或者其他权力机构和个人,向人们揭示话语、符号与权力之间的关系,达到对现有社会制度和意识形态进行解构的目的,Martin称之为CDArealis(已然的批评话语分析);而与之构成互补关系的另一方面则是Martin主张的CDAirrealis(未然的批评话语分析)。他认为话语分析应该采取积极友好的态度。这个积极友好,不仅适用于自己和自己一方的人,而且适用于自己的对立一方,其目标在于通过这样的分析,朝着"和平语言学"(peacelinguistics)的远大目标努力,最终建成一个宽松、和解、共处的人类社会,这就是,积极话语分析(简称PDA)。

简介：<正>Inthispaper,weprovethatthesetofallfactorizationindicesofacompletelypositivegraphhasnogaps.Inotherwords,wegiveanaffirmativeanswertoaquestionraisedbyN.KoganandA.Berman[8]inthecaseofcompletelypositivegraphs.

简介：在这个工作，我们主要为一个拓扑地动态的系统调查复杂性的问题(X，f)。如果，我们证明f有一个完整的措施中心在那里存在X的一个可计算的底令人满意为任何i，在X有y以便N(y，Ui)积极Banach上面的密度被设置。而且，我们考虑混乱性质(X，f)。如果它及物然而并非最小，我们证明如此的一个系统在拿窄小的街道的意义是混乱的。

简介：