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简介：目的：观察针剌对便秘大鼠模型排便功能的影响。方法：利用复方地芬诺酯复制便秘大鼠模型，观察针刺“合谷”、“后三里”对大鼠首次排便时间、12h排便粒数和排便重量的影响。结果：模型组大鼠首次排便时间延长、大便粒数和重量显著减少；针刺治疗后，便秘大鼠首次排便时间显著缩短，12h大便重量和粒数明显增加。结论：针剌能够显著改善便秘大鼠的排便功能。

简介：ObjectiveToExplorethemechanismoftheacupunctureonposanenopausalosteoporo-sis.MethodOnehundredWistarfemalerats(6month-old)wererandomlydividedintotwogroups:O-variectomizedgroupandsham-operatedgroup.After3monthsofoperation,threeratswerechosentomeasurethebonehistomorphometricindexes.Thentheovariectomizedratsweredistributedrandomlytoacupuncturegroupandyigaininggroupandnormalsalinegroupandcontrol2group,theratswererespec-tivelytreatedbyacupunctureandyigainingandnormalsaline.Afterthetreatmentforthreecourse,allratswerecollectedbloodfromtheateriafemoralis,thelevelsofcalcitoninweremeasuredbyIRMA.thebonehistomorphometricindexesandC-cellinthyroidfollicleweredetected.ResultsThelevelsofcalcitoninofacupuncturegroupwereincreasingsignificantlycomparingwiththeyigaininggroupandthenormalsalinegroupandthecontrol2group(p<0.05),buttherewerenoevidentdifferenceinthelevelsofcal-citoninbetweentheacupuncturegroupandthesham-operatedgroup.Themeantrabecularbonewidthandthetmbecuharbonevolume%ofacupuncturegroupwereincreasingmorethanthatofthenormalsalinegroupandthecontrol2group(p<0.05);themeanmarrowcavitywidthandthemarrowcavityvol-ume%werederreasingmorethanthatofthenomalsalinegroupandthecontrol2group(P<0.05);thenumberofC-cellinthyroidfollicleoftheacupuncturegroupwereincreasingmorethanthatoftheothergroupsexceptthesham-operatedgroup.ConclusionAcupuncturecaneffectivelytreatthepost-menopausalosteoporosisbystrengtheningtheabilityoftheC-cellinthyroidfolliclewhichcanimprovethelevelsofcalcitonininserum.

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简介：YoungWistarratsweredividedintosixgroups,theexperimentalgroupsweregivenLa(NO3)3atdoseof20,10,2,0.2,0.1mg*kg-1andthecontrolgroupwasgivenphysiologicalsalinerespectivelyforsixmonths.Theanimalswereweighedandtheratiosofthelivertobodyweightwerecounted.Pathologicalchangesofliverwereobservedbylightmicroscopeandtransmissionelectronmicroscope.Glutamic-oxalacetictransaminase(GOT),glutamic-pyruvictransaminase(GPT),gamma-glutamyltransferase(γ-GT)andalklinephosphatase(ALP)intheserumweremeasured.Theresultsindicatethatthebodyweightofanimalsgainesslowlyinthegroupof20mg*kg-1La(NO3)3,butitgainedquicklyintheratsfedwith0.1mg*kg-1La(NO3)3.Biochemicalindexeshavenoabnormalchanges.Inthegroupof20mg*kg-1La(NO3)3,therewerelipiddropletsanddecreaseofglycogeninthehepatocytes,densermatrixofthemitochondria,deformationofthenucleiofsomehepatocytestodifferentdegreeandinfiltrationofinflammatorycellsatportalarea.ThemorethedoseofLa(NO3)3weregiventotherats,themorethenumberofbodiescontaininghighlyelectronicdensegravel-likegranulesandthesecondarylysosomeswithdensebodies.Theratsfedwith20mg*kg-1La(NO3)3forsixmonthsshowsinjuriouseffectsonthehepatocytes.

简介：Objective:Toevaluatetheeffectofosteoporosisonfracturehealingthroughobservingthehistomorphologicalchanges,bonemineraldensityofcallusandexpressionanddistributionoftransforminggrowthfactorbeta1(TGF-β1),basicfibroblastgrowthfactor(bFGF)andbonemorphogeneticprotein-2(BMP-2)inovariectomizedrats.Methods:SixtyfemaleSprague-Dawleyrats(aged12weeksandweighing235gonaverage)wererandomlydividedintoanovariectomized(OVX)group(n=30)andasham-operated(SO)group(n=30).OvariectomywasperformedintheOVXratsandsameincisionwasmadeintheSOrats.Threemonthslater,fractureoffemoralshaftwasmadeonalltherats.Thentheywerekilledatdifferenttimepoints.Callusformationwasobservedwithhistologicalandimmunohistochemicalmethods.Results:AreductionincallusandbonemineraldensityinthehealingfemurandadecreaseofosteoblastsexpressingTGF-β1nearthebonetrabeculawereobservedintheOVXrats3-4weeksafterfracture.HistomorphologicalanalysisrevealedahighercontentofsoftcallusintheOVXratsthanthatintheSOrats.ImmunohistochemistryresultsshowedthatnoremarkabledifferenceinexpressionanddistributionofBMP-2andbFGFbetweentheOVXandSOgroupswasfound.Conclusions:Osteoporosisinfluencesthequantityandqualityofcallusduringtheearlyperiodoffracturehealing.TheeffectofosteoporosisonfracturehealinghasnorelationshipwiththeexpressionofBMP-2orbFGF.ThedecreasedexpressionofTGF-β1inosteoblastsmaycauseadecreaseinqualityoffacturehealingafterosteoporosis.

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简介：Objective:Todescribeseveralapproachesofearsurgeriesforexperimentalstudiesinrats.Methods:Anesthetizedratswerepreparedfordemonstrationofvariousearsurgeryapproachesdesignedtooptimizeexperimentaloutcomesinstudieswithspecificgoalsandexposurerequirements.Thesurgicalapproachesincludedtheposteriortympanum,superiortympanum,inferiortympanumandoccipitalapproaches.Results:Themiddleearcavityandinnerearweresuccessfullyexposedfromdifferentanglesviathementionedsurgicalapproaches.Forexample,electrodeplacementforrecordingofcochlearbioelectricresponseswaseasilyachievedthroughtheposteriortympanumorinferiortympanumapproach.Alternatively,drugdeliveryorgenetransfectionviaroundwindowmembranewasmosteasilyaccomplishedusingtheposteriortympanumapproach.Cochlearperfusionofprotectiveorototoxicdrugswasbestperformedusingtheinferiortympanumapproach.Ossicularchaininterruptiontoinduceaprolongedconductivehearinglosswasreadilyachievedusingasuperiortympanumapproach.Lastly,surgicaldestructionoftheendolymphaticsactoinduceexperimentalendolymphatichydropswasreadilyperformedviaanoccipitalsurgicalapproach.Conclusion:Thesestandardizedsurgicalapproachescanbeappliedinscientificstudiesoftheearwithdifferentpurposescoveringelectrophysiology,conductivehearingloss,intra-cochleardrugperfusionandexperimentalstudiesrelevanttoMeniere’sdisease.

简介：Objectives:Toexploretheeffectofacupunctureoncerebralinfarctioninratsandtotryprovidingsomeexperimentalparametersforclinicalpractice.Methods:27healthyWistarratswererandomlydividedintopseudo-operation(n=10),model(n=8)andacupuncture(n=9)groups.Neuro-functionaldefectscoring,apoptosisofsinglebrainsliceandthenumberofbcl-2immuno-reaction(IR)-positiveneuronsinCA1areaofthehippocampuswereusedastheindexestoinvestigatethepossiblemechanismsofacupunctureof'NieSanZhen'(needlingthreeacupointsinthetemporalregion)and'SiShenZhen'(needlingfouracupointsintheocciputregion)intreatingratswithcerebralinfarction.Results:Thereexistedsignificantdifferencesbetweenacupuncturegroupandmodelgroupinimprovingneurologicfunctionalactivities,inhibitingapoptosisofthebraincellsandincreasingbcl-2IR-positiveneuronsinthehippocampalCA1area(P＜0.01).Conclusion:Acupuncturetherapycanimprovecerebralinfarctionintheratbysuppressingapoptosisandup-regulationofthebcl-2IR-positiveneuronexpression.

简介：Objective:Toobservetheeffectofacupunctureonhyperleptinaemiaandhyperinsulinemiaforstudyingitsunderlyingmechanismaboutanti-obesityandreducingbloodlipidinobesityrats.Methods:Atotalof80SDratswererandomizedintonormalcontrol,model,acupunctureandmedicationgroups,with20casesineachgroup.Hypothalamicobesitymodelwasestablishedbysubcutaneousinjectionof15%sodiumglutamate(0.2mL/10gbodyweight),oncedailyandcontinuouslyfor5days.'Zusanli'(足三里ST36),'Sanyinjiao'(三阴交SP6),'Guanyuan'(关元CV4)and'Zhongwan'(中脘CV12)werepuncturedandstimulatedelectrically(100Hz,dense-sparsewaves,andasuitablestrengthinducinglocalmusculartremor)for15min,oncedaily.Inmedicationgroup,ratswerefedwithSibutramine4mg/kg,oncedaily.After4weeks'treatment,Lee'sindexwasdetected,andserumleptinandinsulincontentsweredeterminedbyradioimmunoassay(RIA).Results:Comparedwithnormalcontrolgroup,Lee'sindex,serumleptinandinsulincontentsofmodelgroupincreasedsignificantly(P<0.01).Comparisonbetweenacupunctureandmodelgroups,Lee'sindexandserumleptinofacupuncturegroupdecreasedsignificantly(P<0.01),andseruminsulinlevelalsolowered.Incomparisonwithmodelgroup,Lee'sindex,serumleptinandinsulinlevelsofmedicationgroupalsolowered.ComparisonbetweenacupunctureandmedicationgroupsshowedthatLee'sindexandserumleptinlevelofacupuncturegroupweresignificantlylowerthanthoseofthelatergroup(P<0.01),displayingthatthetherapeuticeffectofacupunctureisbetterthanthatofSibutramineinloweringLee'sindexandserumleptin.Nosignificantdifferenceswerefoundbetweenacupunctureandmedicationgroupsinbodyweightandlengthandseruminsulin(P>0.05).Conclusion:AcupuncturecaneffectivelyreduceLee'sindex,serumleptinandinsulincontentsinfastingobeserats,whichmaycontributetoitseffectinanti-obesity.

简介：·AIM:Toestablishanewmethodtorecordvisualevokedpotentialofratsandthismethodwasappliedtoretinalconedegeneration(RCD)andcongenitalstationarynightblindness(CSNB)ratstoseewhetheritisfeasible.·METHODS:Afteranesthesia,theratsscalpsurfacewasexposedandtwostainlessscrewswereimplantedat4mminfrontofand8mmbehindBregma.Thefrontonewasreferenceelectrodeandthelatteronewasrecordingelectrode.Thegroundelectrodewasjoinedtothetail.AndwerecordedVEPofscotopicadaptationandphotopicadaptation(Thestimulusintensityoflight:0.011,0.035,0.11,0.35,1.1and3.5cd·s/m2.Theywerealloverlappedfor100times.Therewere2-5minutesintervalsbetweendifferentintensities.Thefrequencyofstimulationwas1Hzandtimecoursewas300milliseconds).Aweeklater,werecordedtheVEPagaininthesameconditions.·RESULTS:Theratswhowereimplantedwithelectrodescouldlivelonger.Whentheywererecorded,therewasnodisturbanceandthewaveformswereveryclearandsteady.Therewasnodifferencebetweenthewaveformsrecordedbyconventionalmethodandthosebyimplantedelectrodeswhiletheamplitudeofnewmethodwaslargerthanconventional.WecouldgetthesameconclusioninRCDandCSNBrats.·CONCLUSION:Sincetheelectrodescanbekeptonthescalpforalongertime,wecannotonlyobservedynamicchangesofVEPbutalsoobservethelong-durationpatho-logicalchangesintherats.Becausethepositionsofrecordingandreferenceelectrodeswerefixed,thesystemerrorcanbereducedandtherepeatabilityofexperimentsincreased.·

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简介：客观：为了探索颈的方面关节的神经分布的模式并且从方面关节决定小径到背面的根中心(DRG)以便澄清，膜弥漫的颈疼痛，头疼，和肩膀疼痛使用。方法：42男Sprague-Dawleyrats，称250-300g，随机被划分成三个组：组A(n=18)，组B(n=18)，和组C(n=6)。在有intraperitonealpentobarbital钠(45mg/kg身体重量)的麻醉下面，背面的纵的切口在颈的脊骨上被做在一台显微镜下面暴露所有老鼠的左颈的方面关节囊的一条中线。在组A的老鼠在组B和组C经历了交感神经切除术，而是老鼠没经历交感神经切除术。然后，0.6μl5%bisbenzimide(Bb)被注入C_(1-2)，C_(3-4)和方面在组A和组B分别地6只老鼠连接的C_(5-6)。洞立即与矿物质蜡被封上阻止Bb和扁带的漏，皮被闭上。但是在组C，0.9%正常盐被注入相应的联合的囊。然后在有intraperitonealpentobarbital钠(45mg/kg身体重量)的深重新麻醉下面，在所有老鼠的C_1-C_8左DRG和在组B的同情的中心被获得，标记的神经原的数字是坚定的。结果:用Bb标记的神经原在组B在在组A和组B，和同情的中心的C_1-C_8DRG是在场的。在C_(1-2)和C_(3-4)亚群，标记的神经原从C_1toC_8DRG是在场的，当在C_(5-6)亚群他们从C_3到C_8时。没有交感神经切除术，在交感神经切除术以后的Bb(+)神经原的数字不在从那的注射水平是显著地不同的。但是在其它铺平，在交感神经切除术以后的Bb(+)神经原的数字是显著地不到那没有交感神经切除术。结论：颈的方面关节的神经分布从感觉、同情的神经系统被导出，并且DRG在中央神经系统外面通过神经纤维与同情的中心被联系。

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简介：Thepresentstudywasconductedtoinvestigatethepotentialembryo-fetaltoxicityofArtesunate,anantimalarialdrug,inSprague-Dawleyrats.Thetestitemwasorallyadministeredbygavagetopregnantrats(22femalespergroup)fromgesta-tionalday(GD)6through15atdoselevelsof0,2,4and8mg/kg,AlldamsweresubjectedtocaesareansectiononGD20andtheirfetuseswereexaminedforexternal,visceral,andskeletalabnormalities.Therewerenotreatment-relatedclinicalsigns,bodyweightsandfoodconsumptioninanyofthetreatmentg...

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简介：客观：在老鼠在试验性的针的绳索损害(SCI)上观察橡黄素的效果。方法：六十只Sprague-Dawley老鼠随机被划分成四个组；仅仅为laminectomy的组A，为有SCI的laminectomy的组B，为有为SCI和intraperitoneal注射的200mg/kg橡黄素和组D的一丸大丸药的SCI和intraperitonealinjection的组C盐。SCI模型被使用修改侨民做“T_(12)上的s方法。每个组的六只老鼠在损害和免费的铁的层次以后在4h被打死，深奥针的绳索片断的malondialdehyde(MDA)被bleomycin和thiobarbituric酸(TBA)测量试金独立。后部的手足功能的Therecovery被修改Tarlov估计“在在SCI以后的7d，14d和21d的s规模和斜面方法。损坏针的绳索的组织学的变化也在SCI以后在7d被检验。结果：在SCI以后，免费的铁和MDA的层次显著地在组B和D被增加，当时不在组C。修改Tarlov“s分数和斜面角度显著地在组B，C和D被减少。组织学的调查结果没被改进。结论：在SCI以后，橡黄素能每氧化减少类脂化合物的水平，然而并非改进功能的恢复。

简介：ObjectiveTostudylocalinflammatoryresponseafterimplantationofhydroxyapatitesyntheticossicularprosthesis.MethodsHydroxyapatitegranuleswereimplantedinthebullain32rats.Shamsurgicalprocedureswereperformedin10ratsasthecontrol.Animalsweresacrificedat1to300daysaftersurgery.Bullasections,stainedwithHEandMallory'sazan,wereexaminedfornumbersandpercentagesofvariousinflammatorycelltypes.ResultsSlightlymoreinflammatoryreactionwasseeninanimalswiththeimplantthaninthecontrols,mostlyduringtheearlystagefollowingtheimplantationprocedure.Fewinflammatorycellswereobservedatlatertimes.Thereweresatisfactoryfibrosisinbothimplantedandcontrolears.ConclusionTheresultsindicatethathydroxyapatitesyntheticprosthesisisabiocompatibleimplantationmaterialinthemiddleear.Nonetheless,thepresenceofinflammatoryreactionimmediatelyfollowingimplantationimpliesthatcontrolofinfectionisimportantintheearlytimesaftertheimplantationprocedure.