简介：客观：在在人的创伤的奔流和正常透镜的上皮的房间之间的原子factor-KB(NF-κB)的表示学习差别。方法：全部的RNAof前面的囊标本从受不了创伤的奔流做半量的RT-PCR并且在在他们之间的NF-κB的表示进行差别的分析的正常cadaveric眼睛施主和那些在显微镜下面被拿。结果：作为与在正常控制组的0.8337的平均数相比，NF-κB的表示等价物为在创伤的奔流患者的透镜的上皮的房间是0.9074，并且差别具有显著意义(t=2.447，P<0.05)因此。结论：NF-κB是可能的对必要的一种抄写因素维持正常透镜的上皮的房间的新陈代谢。在创伤的奔流患者可得到的更高的NF-κB“透镜的上皮的房间工具NF-κB具有到创伤的奔流的出现和开发的可能的关联的s。

简介：Objective:Toinvestigatetheproliferativeeffectofkeratinocytegrowthfactor(KGF-2)onhumanadultkeratinocytes.Methods:Thestandardmediumwaskeratinocytegrowthmediumwithoutbovinepituitaryextract(BPE),hydrocortisoneorepidermalgrowthfactor(EGF).Keratinocytesfroma48-year-oldsubjectwereculturedandseededondisheswithstandardmediumofEGFincelldensityof2×104/32mm2.After24hours,themediumwasreplacedbythestandardmediumwith0,4,16,125and500ng/mlKGF-2,respectively.ThestandardmediumwithEGFwasusedasthepositivecontrolandthestandardmediumwithoutEGForKGF-2wasusedasthenegativecontrols.Thegrowthofkeratinocyteswasmonitoredby3-(4,5-dimethythiazol-2-yl)-2,5dipheyltetrazoliumbromide(MTT)assayandbyphotographsondays3,5and7,respectively.Results:KGF-2inconcentrationsof4-500ng/mlshowedasignificantproliferativeeffectondays5and7ascomparedwiththatofthenegativecontrols(P<0.01).Onday3thecellswereproliferatedto1.5-2.5-fold,onday5to3-5-foldandonday7to3-12-foldinKGF-2mediumasthatofthenegativecontrols.TheoptimalresponseoccurredwhentheconcentrationofKGF-2was125ng/mlonday7.CellproliferationwasalsoconsistentlyhigherinallKGF-2concentrationsascomparedwiththatofthepositivecontrols.Conclusions:KGF-2hassignificanteffectsontheproliferationofadultkeratinocytes,whicharemoreeffectivethanthatofEGF.ThisstudysupportsKGF-2canimprovethehealingofchronicwoundsinadultsinclinic.

简介：Objective:Toexaminetheeffectsofratmarrowstromalcells(rMSCs)ongeneexpressionoflocalbrain-derivedneurotrophicfactor(BDNF)andnervegrowthfactor(NGF)afterinjectionofrMSCsintoCisternMagnumofadultratssubjectedtotraumaticbraininjury(TBI).Results:GroupcelltransplantationhadhigherBDNFandNGFgeneexpressionsthanGroupsalinecontrolduringaperiodoflessthan3weeks(P<0.05).Conclusions:rMSCstransplantationviaCisternMagnuminratssubjectedtotraumaticbraininjurycanenhanceexpressionsoflocalbrainNGFandBDNFtoacertainextent.

简介：

简介：Toexplorethemolecularmechanismoftheprotectiveeffectofnervegrowthfactor(NGF)oninjuredspinalcord.Methods:TheposteriorT8(the8ththoracicsegment)spinalcordsof60Wistarratswereinjuredbyimpactscausedbyobjects(weighing10g)fallingfromaheightof2.5cmwithAllensway.Solutionwithnervegrowthfactors(NGF)wasgivento30rats(theNGFgroup)throughamicrotubuleinsertedintothesubarachnoidcavityimmediately,andat2,4,8,12and24hoursafterspinalcordinjury(SCI)respectively.Normalsaline(NS)withsamevolumewasgiventotheother30rats(theNSgroup)withthesamemethod.And5normalratsweretakenasthenormalcontrols.Theexpressionofbcl-2andbaxproteinsinspinalcordwasdetectedwithimmunohistochemistry.Theapoptoticneuronsinspinalcordweremeasuredwithterminaldeoxynucleotidyltransferase-mediateddUTP-biotinnickend-labelingofDNAfragments(TUNEL)staining.Results:Thepositiveexpressionofbcl-2proteinwasstronginthenormalcontrols,butdecreasedintheNSgroup,andincreasedsignificantlyintheNGFgroupascomparedwiththatoftheNSgroup(P<0.01).Thepositiveexpressionofbaxproteinwasalsostronginthenormalcontrols,butincreasedintheNSgroup,anddecreasedsignificantlyintheNGFgroupascomparedwiththatoftheNSgroup(P<0.01).ApoptoticneuronswerefoundintheNSgroup,andtheydecreasedsignificantlyintheNGFgroupascomparedwiththatoftheNSgroup(P<0.01).Conclusions:NGFcanprotecttheinjurednervetissuesthroughstimulatingtheexpressionofbcl-2protein,inhibitingtheexpressionofbaxproteinandinhibitingtheneuronalapoptosisafterSCI.

简介：Objective:ToexploretheexpressionofmRNAanditsproteininburnedratsandtheireffectsofburnwoundhealing.Methods:Apartial-thicknessburnof30%totalbodysurfaceareawascreatedonthebackof40Wistarrats.Insituhybridizationandimmunohistochemicalmethodswereusedtoexaluatethelocationandtheamountofthec-fosmRNAanditsproteininnormalskinandtheburnedskin,respectively,at3h,6h,1d,3d,7dand14dafterburn.Results:Underalightmicroscope,boththeexpressionofc-formRNAanditsproteincouldbefoundinthenormalskin,buttheirinductionlevelsweremuchhigherintheburnedskin.Thelevelofforproteinexpressionreachedpeakat3hafterburnwhilethatofc-formRNAreachedpeakat6hafterburn.Conclusions:Theexpressionofc-foscanbeinducedbyburns.Andthepeaklevelexpressionofc-formRNAcomeslaterthanthatofc-fosprotein.Itindicatesthattheactionoffosproteinisinducedbypost-translationalmodificationofpre-existingfosmolecules.

简介：ToinvestigateeffectsofShenfuinjectionontheconcentrationsofplasmatumornecrosisfactor-alpha(TNF-α)andinterleukin-6(IL-6),activityofNuclearFactorkappaB(NF.gB)andhearttissueultrastructureduringmyocardialischemia/reperfusion(I/R)injuryinratsanditspotentialmechanism.Methods:Myocardialischemia/reperfusion(I/R)wasproducedbyligationandreleaseoftheleftanteriordescendingcoronaryartery.Ischemialastedfor30minandreperfusionfor60min.Twenty-fourhealthymaleSDratsweighing230-280gwererandomlydividedintothreegroups(n=8,each):GroupⅠ(Sham-operationgroup);GroupⅡ(I/Rgroup);GroupⅢ(Shenfugroup),inwhichShenfuinjection(10ml/kg)wasintraperitoneallyinjected30minbeforeischemiainanimalswithI/R.TheplasmaconcentrationsofIL-6andTNF-αweremeasuredbyELISA,andtheheartwasharvestedfordeterminationofNF-κBlevelsbyEcl-westernblotanalysis.Electronmicroscopywasusedtostudyitsultrastructure.Results:Afterreperfusion,NF-κBbindingactivityinmyocardialnucleiandtheplasmaconcentrationsofIL-6andTNF-αweresignificantlyincreasedinGroupⅡ,comparedwithGroupⅠ(P<0.01),andtheyweremarkedlyreducedinGroupⅢ,comparedwithGroupⅡ(P<0.01).Inaddition,electronmicroscopicexaminationshowedmoreseriousinjuryofthemyocardiumultrastructureinGroupⅡ,whileinGroupⅢthemyocardialultrastructurewassimilartonormalstate.Conclusions:ShenfuinjectioninhibitsNF-κBactivityinI/Rmyocardiumandleadstodown-regulationofproinflammatorycytokineexpression,whichmightbeoneofthemolecularmechanismsofShenfuinjectionincardioprotection.

简介：Objective:TostudythesequentialchangesofHIF-1α(hypoxia-induciblefactor1alpha)inexperimentalspinalcordinjuryinratsandtoanalyzeitspotentialeffectsinSCI.Methods:AstaticcompressionmodelofSCIwasemployedinthisstudy.ExpressionsofHIF-1αweremeasuredwithimmunohistochemicalstaining,whileflowcytometrywasusedtodeterminetheapoptoticratioandbcl-2expressions.Results:HIF-1αbegantoincrease1dayafterinjury,andreachedthepeakat3-7days.Twoweekslater,itdeclinedsignificantly.ThesequentialchangesofHIF-1αcoincidedwellwiththealterationsofapoptoticratioandcontentsofbel-2.Conclusions:HIF-1αpossiblyparticipatesinthesecondaryischemicandhypoxicproceduresafterspinalcordinjury,andmaymediatethetraumaticapoptosis.FurtherunderstandingofHIF-1αmayprovidenewtherapeuticregimensforSCI.

简介：探索骨头的效果的目的有带hepatocyte生长因素的adenoviral向量的导出髓的间充质的干细胞(BMSC)transfected(HGF，Ad-HGF)在灼伤创伤愈合上。从男Wistar老鼠的方法BMSC用由密度坡度centrifugation中等、与包含20%胎儿的牛的浆液(FBS)的DMEM有教养的Percoll分开被分开并且净化。当时，BMSC是有在感染(MOI)的100复合的最佳的基因transduction效率的Ad-HGF的transfected。transfection和在暂停的HGF的表示的效率被流动cytometry检测，酶分别地连接了immunosorbent试金(ELISA)。32只雌老鼠受到90慥?灳瑩吗？

简介：Objective:　Tocomparethedynamicchangesofinterleukin-1(IL-1),interleukin-6(IL-6),andtumornecrosisfactor(TNF)inintermingledskingraftwiththoseinothertypesofskingraftsinrats.　　Methods:　A10%-15%third-degreeburnwascreatedin180Spregue-Dawley(SD)rats.Afterremovingthescar,skingraftswereperformedontheopenwoundsimmediatelywithautoskin(aus,n=54),alloskin(als,n=54)andintermingledskin(n=36).Thatistosay,intheintermingledskingraft,abigpieceofalloskin(mals)wasgraftedfirst,and3dayslater,smallpiecesofautoskin(maus)wereembeddedinthealloskin.Therest36ratsweretakenasthecontrols.AndthebiologicalactivitiesofIL-1,IL-6andTNFingraftsheetsineachgroupweredetectedafterskingraft.　　Results:　ThelevelsofIL-1,IL-6andTNFintheausgroupdecreasedsteadilyaftertheirinitialelevations,whereasinthealsgrouptheyincreasedsignificantlyandkeptonthepeaklevelinthelaterphases.Intheintermingledgroup,thereappearedalowestIL-1levelinthemalsandahighestoneinthemaussimultaneouslyat7(4)days(Thenumberoutofparenthesisisthedaysaftertransplantingwithalloskinsheets,andthenumberinparenthesisisthedaysafterembeddingautoskinsheetsintheintermingledskingraft.Similarlyhereinafter.)afterskingraft(P<0.01),andthehighlevelinthemausabruptlydecreasedat14(11)daysafterskingraft.Atexactlythesamephaseonday7(4),aprominentpeakedIL-6inthemalsoccurred.Inthelaterphases,thelevelsofTNFremainedrelativelylowbothinthemalsandinthemaus.Fromday7(4)on,eachcytokinefluctuationinthemalssynchronizedwiththatinthemaus.Thelongertheposttransplantationperiodlasted,themorethepositivecytokinecorrelatedbetweenthemalsandthemaus.　　Conclusions:　ThelowlevelsofIL-1andTNFmaybeimportantfactorstolightentheintensityoflocalrejectionintheintermingledskingraft.Thetempo

简介：Objective:ToinvestigatetheeffectofsubstanceP(SP)ongeneexpressionoftransforminggrowthfactorβ-1(TGFβ-1),transforminggrowthfactorreceptor-1(TGFR-1)andtransforminggrowthfactorreceptor-2(TGFR-2)infibroblastsculturedinvitrofromrat'sgranulationtissues.Methods:Thefibroblastsfromthegranulationtissuesintheskeletalmuscleofrat'shindlimbsinjuredbyformaldehydewereculturedinvitro.Whendifferentconcentrations(10-9-10-5mol/L)ofSPwereaddedintotheculturemedium,thechangesofgeneexpressionofTGFβ-1,TGFR-1andTGFR-2intheculturedfibroblastswereobservedwithreversetranscriptionpolymerasechainreactionatdifferentintervals(0,3,6,12and24hoursafterincubation).Results:ThegeneexpressionofTGFβ-1,TGFR-1andTGFR-2inthefibroblastsculturedfromrat'sgranulationtissueswasup-regulatedbySP.ThepeaklevelofthemRNAexpressionwasfoundat10-8mol/LSPandtheup-regulationeffectwasnotfoundat10-5mol/Land10-6mol/L.ThepeaklevelsofgeneexpressionofTGFβ-1,TGFR-1andTGFR-2inthefibroblaststreatedwithSPwereachievedat6and12hours,respectively.Conclusions:SPhasup-regulationeffectonthegeneexpressionofTGFβ-1,TGFR-1andTGFR-2infibroblastsfromrat'sgranulationtissuesinvitro,andtheeffectisrelatedtodifferentstimulatingconcentrationsofSP.Itmaybeconcernedwithproliferationanddifferentiationoffibroblastsandformationofscartissuesduringwoundhealing.

简介：Objective:Tostudytheeffectofnervegrowthfactor(NGF)andSchwanncellsonaxonregenerationoftheinferioralveolarnervefollowingmandibularlengtheningwithdistractionosteogenesis.Methods:Unilateralmandibularosteodistractionwasperformedin9healthyadultmalegoatswithadistractionrateof1mm/d.Every3goatswerekilledondays7,14and28aftermandibularlengthening,respectively.TheinferioralveolarnervesinthedistractioncalluswereharvestedandprocessedforultrastructuralandNGFimmunohistochemicalstudy.Theinferioralveolarnervesfromthecontralateralsidewereusedascontrols.Results:Onday7afterdistraction,axondegenerationandSchwanncellproliferationwereobserved,andverystrongstainingofNGFinthedistractednervewasdetected.Onday14afterdistraction,axonregenerationandremyelinationwereeasilyobserved,andNGFexpressionstartedtodecline.Onday28afterdistraction,thegrayscaleofNGFimmunoreactivityrecoveredtothenormalvalueandtheSchwanncellsalmostrecoveredtotheirnormalstate.Conclusions:Gradualmandibularosteodistractioncanresultinmildormoderateaxondegenerationoftheinferioralveolarnerve.NervetraumamaystimulatetheproliferationofSchwanncellsandpromotethesynthesisandsecretionofNGFintheSchwanncells.SchwanncellsandNGFmightplayimportantrolesinaxonregenerationoftheinjuredinferioralveolarnervefollowingmandibularlengthening.

简介：ToexploretherelationshipbetweensubstanceP(SP)releasedfromperipheralnerveendingsandtheexpressionofepidermalgrowthfactor(EGF)andepidermalgrowthfactorreceptor(EGFR)duringwoundhealing.Methods:FiftyWistarratswererandomlydividedinto2groups,injurygroupandcapsaicingroup.Intheinjurygroup,afull-thicknessskinwoundonthebackoftheratwastaken.Thewoundedgeandgranulationtissuesweretakenonthe1st,3rd,6th,9th,12thdaysafterinjury,respectively.Inthecapsaicingroup,capsaicinwasinjectedsubcutaneouslyonthebackoftheratstodestroythesensorynervetopreventthesecretionofSP,thenawoundandsamplewasmadeinthesameway.ImmunohistochemistryandinsituhybridizationwereemployedtodetecttheexpressionofSP,EGF/EGFR,andEGFmRNA/EGFRmRNAinthegranulationtissues.Results:Intheinjurygroup,immunohistochemicalstainofSPandEGF/EGFRwaslocatedonthehairfolliclesandsebaceousglandsatthe1stday.AndthestainofSPwasobviousatthe3rddayinthegranulationtissues,thendecreasedgradually.EGF/EGFRwasatlowlevelatthe3rdday,thenincreasedgraduallyandreachedthepeakatthe9thday,thendeclined.Inthecapsaicingroup,theimmunohistochemicalstainofSPandEGF/EGFRwasfaintandwithoutobviouschangeduringthewoundhealingprocess.ThetendencyoftheEGFmRNA/EGFRmRNAexpressionwassimilartothatofEGF/EGFR.Conclusions:Duringwoundhealing,SPmaypromotethehealingprocessbyaffectingtheexpressionofEGF/EGFRinthegranuationtissues.

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简介：Objective:Toinvestigatetheneointimaformationandtheexpressionofmonocytechemoattractantprotein-1(MCP-1)andtumornecrosisfactor-α(TNF-α)incuff-inducedvascularinjuryinmousemodel,andtoexaminetheeffectofangiotensinIItype1receptor(AT1)blocker,olmesartan,onMCP-1andTNF-αexpressionandconsequentlyvascularremodeling.Methods:Vascularinjurywasinducedbypolyethylenecuff-placementaroundthemousefemoralartery.SomemiceweretreatedwithAT1receptorblocker,olmesartan,atthedoseof3mg*kg-1*day-1withanosmoticminipump.Neointimaformationandtheproliferationofvascularsmoothmusclecells(VSMCs)weremeasuredbymorphometricanalysisandbromodeoxyuridine(BrdU)incorporation.MCP-1andTNF-αexpressionwasdetectedbyWesternblotandimmunohistochemicalstaining.Results:Weobservedneointimaformation14daysaftercuffplacementaswellasVSMCsproliferationinthemediaandneointima.CuffplacementalsoinducedMCP-1andTNF-αexpressioninthemediaandneointimathattheVSMCsspecificallyexisted.Treatmentofmicewitholmesartanatadoseof3mg*kg-1*day-1,whichdidnotinfluencesystolicbloodpressure,significantlydecreasedneointimaformationandtheproliferationofVSMCs.OlmesartanalsoinhibitedMCP-1andTNF-αexpressionintheinjuredarteries.Conclusions:OurresultsdemonstratethatblockadeofAT1receptorinhibitsMCP-1andTNF-αexpressionandtherebyimprovesvascularremodeling.

简介：Objective:Toinvestigatethebiologicalfunctionofplatelet-derivedgrowthfactorB(PDGF-B)onthesurvivalandproliferationofcatcornealendothelialcellssoastoprovidebasesforfurtherstudiesofitsroleinwoundrepairanditsclinicalapplication.Methods:TotalRNAwasextractedfromtheplacentatissuesofhealthypregnantwomenundergoinghysterotokotomyandPDGFeDNAwasobtainedwithre-versetranscription-polymerasechainreaction(RT-PCR).TheprokaryoticexpressionvectorpET-PDGF-BwasconstructedandexpressedtherecombinantPDGF-BinEscherichiacoli(E.coli)BL21(DE3).AfterpurificationandrefoldingonNi2+-chelationaffinitychromatography(NTA)column,itwasusedtoculturecatcornealendothelialcells.Cellproliferationwastestedbymodifiedtertrazoliumsalt(MTT)andflowcytometer.Andthemorphologicchangeandtheultrastructurewereob-servedunderaninvertedphasecontrastmicroscope,ascan-ningelectronmicroscopeandatransmissionelectonmicroscope,respectively.Results:PDGF-Bchainpeptide(PDGF-BB)genewassuccessfullyinsertedintotheprokaryoticexpressionvector,pET-28a(+).ThepurifiedrecombinedproteinpET-PDGF-Bshowedasinglebandonsodiumdodecylsulfatepolyacry-lamidegelelectropheresis(SDS-PAGE)withthemolecularweightofabout27u,whichwasinagreementwiththede-ducedvalue.MTTandflowcytometryshowedthatPDGF-BBpromotedthesurvivalandproliferationofcatcornealen-dothelialcells.Conclusions:Theconstructionofrecombinantprokary-oticexpressionvectorpET-PDGF-BandthepreparationofPDGF-BBproteinprovideafoundationforfurtherstudyofthefunctionofPDGF-BBandproducingbiologicalPDGF-BBprotein.TheexpressedPDGF-BBpromotestheprolif-erationofculturedcatcornealendothelialcells.

简介：Objective:Toinvestigatetheexpressionandpatternofvascularendothelialgrowthfactor(VEGF)anditsfetalliverkinase-1(Flk-1)receptorinspinalcordanddorsalrootgangliaafterneurotomyofsciaticnerveinrats.Methods:Forty-fiveadultmaleWistarratsweredividedrandomlyintoacontrolgroup(n=5)andanexperimentalgroup(n=40).ThebilateralsciaticnervesoftheratsintheexperimentalgroupunderwentneurotomyandtheL4-L6spinalcordandthecorrespondingdorsalrootgangliawereharvestedrespectivelyat8hours,and1,3,5,7,10,14and21days(8subgroupswith5ratseach)afteroperation.Theratsinthecontrolgrouponlyunderwentanexposureofsciaticnervewithoutneurotomy.ImmunohistochemistryandimageanalysiswereusedtostudytheexpressionofVEGFanditsFlk-1receptor.Results:BothVEGFandFlk-1receptorexpressedinthenormalratspinalcordanddorsalrootganglia.Inresponsetoneurotomy,theirexpressionreachedahigherlevelandpersistedforashorttimethendeclinedtothenormallevelrapidly.Besides,positivestainingofFlk-1wasobservedinbothglialcellsandnervefibers,whichlocatedinthewhitematterofthespinalcord.Conclusions:VEGFcanpromotetheregenerationofperipheralnervesfromtheangleofcentralneurons,whichestablishestheexperimentalandtheoreticalfoundationforVEGFtreatingperipheralnerveinjuries.