学科分类
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7 个结果
  • 简介:在油煎食物的情况中的集体转移参数(有效潮湿扩散性,集体转移Biot数字和集体转移系数)的知识的精确性,为设计必要、重要,建模并且过程优化。这研究被承担为在在150,170和190点在葵花油球形的米饭脆饼油煎期间决定集体转移参数开发一条途径????颏?蒚郧?髧?鯥蒚觩骮鋥???芀吗?

  • 标签: 传质系数 油炸时间 米果 测定 水分扩散 油炸温度
  • 简介:三个米饭变化,Zhonghan3,Shanyou63和Aizizhan,当在检测微分活跃甲基的材料骑车并且响应干旱应力转移相关基因表示,被使用。实验被在干旱的条件下面的微分显示器技术与10%PEG6000答案模仿了的基因薄片和mRNA执行。结果显示甲基周期能在Zhonghan3和Shanyou63的叶子被激活,但是在干旱应力下面在Aizizhan的叶子禁止了。而且,干旱应力能导致很多methyltransferase基因的表示,特别Rubisco蛋白质methylation的抄写联系了基因,它是有益的因为Rubisco蛋白质氧化和降级的预防,和干旱应力能禁止DNAmethyltransferase基因和histonemethyltransferase基因的抄写。这结果证实活跃甲基骑车并且转移相关基因涉及米饭干旱抵抗。

  • 标签: 干旱胁迫 水稻叶片 基因表达 活性 移相 MRNA差异显示技术
  • 简介:为了在不同发育阶段调查织物transglutaminase活动,叶子和米饭的年轻圆锥花序的变化,从Honglian-typecytoplasmic不育系,YuetaiA和它的维护者线被切除,YuetaiB分别地。为在米饭的织物transglutaminase活动察觉的一个ELISAmeasurement协议很好被建立。结果显示织物transglutaminase活动被钙阳离子,和织物transglutaminase活动在衰老的叶子断然调整在年轻叶子比那显著地高。没有不同差别在YuetaiA和YuetaiB之间被注意。而且,从四个起核心作用到双性人织物transglutaminase活动逐渐地增加了的阶段年轻圆锥花序发展并且直到在双性人的最大值的withtheprogression起核心作用在YuetaiA.However的舞台,没有类似的变化在YuetaiB被观察。这显示tissuetransglutaminase涉及房间在败育花粉的规划死亡。

  • 标签: 水稻 细胞质雄性不育性 程续细胞死亡 圆锥花序
  • 简介:EliminationoftheCRISPR/Cas9constructsineditedplantsisaprerequisiteforassessinggeneticstability,conductingphenotypiccharacterization,andapplyingforcommercializationoftheplants.However,removaloftheCRISPR/Cas9transgenesbygeneticsegregationandbybackcrossislaboriousandtimeconsuming.WepreviouslyreportedthedevelopmentofthetransgenekillerCRISPR(TKC)technologythatusesapairofsuicidegenestotriggerself-eliminationofthetransgeneswithoutcompromisinggeneeditingefficiency.TheTKCtechnologyenablesisolationoftransgene-freeCRISPR-editedplantswithinasinglegeneration,greatlyacceleratingcropimprovements.Here,wepresentedtwonewTKCvectorsthatshowgreatefficiencyinbotheditingthetargetgeneandinundergoingself-eliminationofthetransgenes.ThenewvectorsreplacedtheCaMV35SpromoterusedinourpreviousTKCvectorwithtworicepromoterstodriveoneofthesuicidegenes,providingadvantagesoverourpreviousTKCvectorundercertainconditions.Thevectorsreportedhereofferedmoreoptionsandflexibilitytoconductgeneeditingexperimentsinrice.

  • 标签: genome EDITING SUICIDE gene TRANSGENE KILLER
  • 简介:Apopulationofricebrownplanthopper(BPH)NilaparvatalugenscollectedfromapaddyfieldinHangzhouwassuccessivelyrearedonsusceptiblericeTaichungNative1(TN1)inalaboratoryfreefrominsecticidesformorethan14generations.Thechangesinsusceptibilitytoinsecticidesandecologicalfitnessondifferentresistantricevarietiesweremonitoredineachgeneration.Theresistanceratiotoimidaclopridsharplydeclinedwiththesuccessionofrearinggenerationswithoutinsecticidesfrom359.94-foldatF1to6.50-foldatF14comparedwiththesusceptiblestrain,andtheresistanceratiotochlorpyrifoswasfrom9.90-foldatF1to5.94-foldatF14.Nymphaldurationandweightsofnewlyhatchedfemaleadultsweresignificantlyaffectedbyricevariety,generationandtheirinteractions,butnymphalsurvivalwassignificantlyaffectedbythegenerationonly.Theratioofbrachypterousadultsinmaleswasaffectedbythegenerationandgeneration×varietyinteraction,whereasnodifferencewasfoundinfemales.Nymphaldurationextendedwithincreasinggenerations,andthefemalenymphaldurationwasshorterinthesusceptiblevarietyTN1thanthoseintheresistantvarietiesIR26andIR36.Inaddition,thefemaleadultweightinTN1washigherthanthoseinIR26andIR36.TheseresultsindicatedthattheresistanceoffieldBPHpopulationtoinsecticideswasreversedafterseveralgenerationsofno-exposuretoinsecticides,andtheecologicalfitnessinTN1washigherthanthoseinIR26andIR36.ThesefindingssuggestedtherationalandreduceduseofinsecticidesincombinationwiththemanipulationofresistantricevarietieswouldbeeffectiveforBPHmanagement.

  • 标签: NILAPARVATA LUGENS insecticide-free ECOLOGICAL FITNESS insecticidal