简介：Thecurrentconceptof“AdoptiveTCellImmunotherapyofCancer”isquitedifferentfromhowitwasoriginallyconceived．Withthedevelopmentofmoderntechnologyinmolecularbiology,cellbiology，immunologyandbiochemistryduringthelasttwentyyearsorso，adoptiveimmunotherapyhasgrownfromitsinitialformofasimple“bloodcelltransfer”intoitspresentprocesswhichinvolveshostvauccination，effectorcellactivation／polarizationandgeneticmodification．Withtheuseofimmuneadjuvantsandtheidentification／characterizationoftumor-reactiveTcellsubsets，orincombinationwithothertherapeuticstrategies，adoptivelytransferredTcellshavebecomemuchmorepotentinmediatingtumorregression．Inaddition，studiesonthetraffickingofinfusedTcells，celltransferperformedinlymphopenicmodels，aswellasthediscoveryofnoveltechniquesinimmunemonitoringforthegenerationofeffectorcellsinvitroandaftercelltransferinvivohaveprovidedusefultoolstofurtherimprovethetherapeuticefficacyofthisapproach．ThisarticlewillreviewtheserelatedaspectsofadoptiveTcellimmunotherapyofcancerwithspecificcommentsoncertaincriticalareasintheapplicationofthisapproach．Withtherapidlyevolvingadvancesinthisarea，itishopedthatthiscellularimmunologictherapyasitwasconceptualizedinthepast，canbecomemoreusefulinthetreatmentofhumancancerinthenearfuture．

简介：TheeffectofPentoxifylline(PTX)ontype1diabeteswasinvestigatedbymeansofthestudiesontheexpressionsofcytokinemRNAinpancreasandtheFas-Fasl,onisletcellsofNODmice.NODmiceweretreatedwithFFXfrom4-6wk,andthenfrom8-12wk.Aftertreatment,itwasfoundthattheincidenceofdiabetesinNODmiceatagesof30wkwasreducedto25%ingroupofmicetreatedwithPTX,incomparisonto73.3%incaseofmiceinjectedwithPBS,andthedegreeofinsulitisinthePTXtreatedmicewaslowerthanthatofthePBSinjectedmice.RT-PCRanalysisrevealeddown-regulatoryeffectontheexpressionsofIFN-γandTNF-αmRNAinPTXtreatedmice,buttherewasnoanyeffectontheexpressionofIL-10.AstotheexpressionofFas,therewasmarkeddecreaseinthemeancytoplasmicintegralopticaldensity(IOD)inPTXtreatedmice,buttherewaslittledifferencebetweenFIXandPBSgroupsintheexpressionofFasL.TheseresultsindicatedthatFIXcouldpreventthedevelopmentofdiabetesinNODmice,whichmightberelatedtotheregulationofTh1/Th2imbalanceandthedecreasedexpressionofFasinisletcells.

简介：TheZ10andZ37strainsofhemorrhagicfeverwithrenalsyndrome(HFRS)virusandtheMongoliangerbil(Merionsunguiculatus)kidneycellswereusedtopreparetheinactivatedbivalentvaccine.AphaseⅡclinicaltrialuseofthisvaccinewasmadein750Chinesevolunteers.Theresultsshowedthatthesidereactionratewas2.5%andthesero-conversionrateofneutralizingantibodiesagainstHantaanandSeoulvirusesintheinoculatedvolunteerswere87.6%and96.3%respectively.