简介：BACKGROUND:Withdevelopmentsoftissueengineeringandgeneticengineering,weaimtoculturemyoblasts,whicharecharacterizedbyhighpurity,highqualityandhighproduction,forwideapplicationinneuralregenerationresearches.OBJECTIVE:Tomodifytraditionaldissociationmethodinordertoobtainmyoblasts,whicharecharacterizedbyhighpurity,highqualityandhighproduction,andexplorethebiologicalpropertiesunderinvitroculture.DESIGN:Observationalstudy.SETTING:BasicInstituteofAcademyofMilitaryMedicalSciencesofChinesePLA.MATERIALS:FourneonatalWistarratsof5daysold,bothgendersandmeanbodymassof10gwereselectedinthisstudy.Themainreagentsanddevicesweredetailedasfollows:DMEMmedium(GibcoCompany),fetusbovineserum(FBS,HycolneCompany),collagenaseⅡ(SigmaCompany),trypsin(SigmaCompany),dispaseⅡ(SigmaCompany),desminantibody(FuzhouMaixinCompany),antibodyⅡandABCkit(WuhanBosterBiotechnologyCompany),deskcentrifuge(KUBATO,Japan),andinvertedphasecontrastmicroscope(LEICADMIRB,Germany).METHODS:TheexperimentwascarriedoutintheBasicInstituteofAcademyofMilitaryMedicalSciencesofChinesePLAfromJunetoOctober2006.Neonatalratsweresacrificedundersterileconditiontoobtainskeletalmusclesoflimbs,whichwerewashedwithcoldPBS(containingbenzylpenicillinandestreptomicina),andmusculartissuewasshearedintopieces.Then,thosemuscularpieceswereaddedwithmixeddigestiveenzyme(containing2g/LcollagenaseⅡ+5g/LdispaseⅡ+0.28g/LCaCl2)astwicevolumeaspieces,dealtwithmechanicalpipettingfor5minutesandculturedinCO2incubatorfor10minutes.Theoperationwasdoneforthreetimesandthemuscularpiecesweredigestedfor45minutesintotal.Moreover,cellsweresuspendedagaininordertoobtainmyoblastsfromskeletalmuscleofneonatalrats.Inaddition,myoblastswerepurifiedwithdifferentialattachmenttechniqueandenzymedigestionsoastoobservemorpho

简介：这糊为为布尔控制的分解w.r.t产量的问题的图理论方法联网的焦点(BCN)。第一由诉诸矩阵和BCN的矩阵表示的半张肌产品，分解w.r.t的定义输出被介绍。秒由指BCN的图形的结构，为分解的一个必要、足够的条件w.r.t产量基于图理论方法被获得。第三，认识到最大的分解w.r.t产量的一个有效算法被建议。最后，一些例子被探讨验证理论结果。

简介：Aftertheoccurrenceofanearthquake,strongmotionobservationnetworkscanrecordgroundmotionatdistributedobservationstations.Basedonthegroundmotionparametersfromtheserecords,thespatialdistributionofseismicintensitycanbequicklydetermined,andthedegreeofdamageindifferentareascanbeestimated.Thisinformationprovidesthetechnicalbasisfortheemergencyresponse,soastoensurethatrescueteamscanreachextremeearthquakeareasandcarryoutthesearchandrescueoperationinanaccurateandtimelymannertoreducecasualtiesandpropertyloss.Inthispaper,weintroduced7intensityalgorithmsandcomparedtheresultswiththerecordsoftheJiuzhaigouMS7.0earthquake.Wefoundthatthedifferencesbetweentheinstrumentalintensitiescalculatedbyeachmethodandthemacrointensitieswerewithina1-degreerange,whichsuggestedgoodpracticalityofthesedifferentmethods.Theresultscalculatedbytheindustrialstandards-basedcalculationmethodandtheintegratedtestshowedgoodconsistency.

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简介：1.IntroductionLetfibeaplanedomainwithsmoothboundaryonandWm,p(fl)betheusualSobolevspaceonnwithnormWhenp=2,pisusuallyomitted.WeshalldenotetheusualinnerproductinL’(fl)orLa(O)’by’,’),andinL’(ofl)by’t’).Weshallusethesamenotationstoindicatethedualltiesbetw...

简介：我们认为在线性、非线性的平常的微分方程(颂诗)的参数评价的问题当模特儿。非线性的颂诗模型广泛地在应用被使用。但是他们的分析答案通常不是可得到的。因此常规的方法通常取决于带巨大的计算费用的数字答案的重复使用。我们建议了在第二个阶段在第一个阶段，和一个数字discretization方法(Eulersdiscretization方法，trapezoidaldiscretization方法，或Runge-Kuttadiscretization方法)包括一个变光滑的方法(变光滑的核或本地多项式试穿)的一条新二阶段的途径。通过数字模拟，我们发现建议方法获得在评价精确性和计算费用之间的合适的平衡。Asymptotic性质也被介绍，它在一些温和条件下面显示出一致性和评估者的asymptotic规度。建议方法以精确性和计算费用与存在方法相比。模拟结果证明有在第二个阶段的在第一个阶段和trapezoidaldiscretization的本地线性变光滑的评估者有最低平均相对错误。我们把建议方法用于HIV动力学数据说明评估者的有实行可能。

简介：PolysaccharidesfromnumeroustraditionalChinesemedicineshavebeenprovenasthebioactiveingredientsandarehenceusedasthequalitycontrolmarkers.However,theassessmentcriteriaalwaysshowapoorspecificity,duetothelackofmaticcomparisonamongtheanalogousherbs.Inthepresentstudy,twosimilarmaterials,namelysea-tangleandsargassum,wereselectedasthemodelherbstodevelopmorespecificmethodsforqualitycontrol.Twowell-establishedmethods,determinationofthetotalpolysaccharidescontentandmonosaccharidescompositionanalysis,werebothemployed.Baseduponthequantitativeresults,theevaluationcriteriaofthepolysaccharidescontentsofnotlessthan2.0%and1.7%wereproposedforsea-tangleandsargassum,tively.NineidenticalmonosaccharidederivativesappearedontheHPLCchromatogramsofthehydrolysisandderivatizedsolutionsofthetwodrugs.Principalcomponentanalysisandorthogonalpartialleastsquaresdiscriminantanalysisusingthepeakareasofmonosaccharidesderivativesasthevariableswereperformed,andtheresultsindicatedthatmannuronicacidandxylosewiththeoppositeconcentrationsinthetwodrugswerethedifferentialcomponents.Adiscriminativecriterionusingthepeakarearatioofthesetwomonosaccharidesderivativeswasproposedforthequalitativeidentification.Inconclusion,amorespecificandquantitativequalitycontrolmethodwasdevelopedforsea-tangleandsargassum.

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简介：Inthispaperwedeveloptwoconformingfiniteelementmethodsforafourthorderbi-waveequationarisingasasimplifiedGinzburg-Landau-typemodelford-wavesuperconductorsinabsenceofappliedmagneticfield.UnlikethebiharmonicoperatorA2,thebi-waveoperator□~2isnotanellipticoperator,sotheenergyspaceforthebi-waveequationismuchlargerthantheenergyspaceforthebiharmonicequation.Thisthenmakesitpossibletoconstructloworderconformingfiniteelementsforthebi-waveequation.However,theexistenceandconstructionofsuchfiniteelementsstronglydependsonthemesh.Inthepaper,wefirstcharacterizemeshconditionswhichallowandnotallowconstructionofloworderconformingfiniteelementsforapproximatingthebi-waveequation.Wethenconstructacubicandaquarticconformingfiniteelement.Itisprovedthatbothelementshavethedesiredapproximationproperties,andgiveoptimalordererrorestimatesintheenergynorm,suboptimal(andoptimalinsomecases)ordererrorestimatesintheH~1andL~2norm.Finally,numericalexperimentsarepresentedtoguagetheefficiencyoftheproposedfiniteelementmethodsandtovalidatethetheoreticalerrorbounds.

简介：Themeanshifttrackerhasdifficultyintrackingfastmovingtargetsandsuffersfromtrackingerroraccumulationproblem.Toovercomethelimitationsofthemeanshiftmethod,anewapproachisproposedbyintegratingthemeanshiftalgorithmandframe-differencemethods.Theroughpositionofthemovingtargetisfirstlocatedbythedirectframe-differencealgorithmandthree-frame-differencealgorithmfortheimmobilecamerascenesandmobilecamerascenes,respectively.Then,themeanshiftalgorithmisusedtoachieveprecisetrackingofthetarget.Severaltrackingexperimentsshowthattheproposedmethodcaneffectivelytrackfirstmovingtargetsandovercomethetrackingerroraccumulationproblem.

简介：在这篇论文，我们描述一个方法由顺序的凸的编程(SCP)解决大规模结构的优化问题。一个预言者修正者内部点方法被使用解决严格地凸的潜水艇问题。SCP算法和拓扑学优化途径被介绍。特别，解决方程的某些线性系统的不同策略被分析。数字结果被介绍为解决拓扑学优化问题显示出建议方法的效率并且比较不同变体。

简介：Themodeloftransientbehaviorofsemiconductorwithheat-conductionisaninitialandboundaryproblem.Alternating-directionmultisteppreconditionediterativemethodsandtheoryanalysesaregiveninthispaper.Electricpotentialequationisapproximatedbymixedfiniteelementmethod,concentrationandheat-conductionequationsareapproximatedbyGalerkinalternating-directionmultistepmethods.ErrorestimatesofoptimalorderinL2aredemonstrated.

简介：Inthispaper,twofinitedifferencestreamlinediffusion(FDSD)schemesforsolvingtwo-dimensionaltime-dependentconvection-diffusionequationsareconstructed.Stabilityandoptimalordererrorestimati-ionsforconsideredschemesarederivedinthenormstrongerthanL~2-norm.

简介：这份报纸涉及线性可变系数的微分代数学的方程(DAE)的解决之可能性和波形松驰方法。大多数以前的工作与光滑的系数和数据集中于线性可变系数的DAE，然而，与相应波形松驰方法的集中率有关的结果都没被获得。在这份报纸，我们为在在传统、最少的广场的Legesguesquare-integrable函数空格的线性可变系数的DAE的解决之可能性理论察觉到的develope，并且为解决线性可变系数的DAE决定波形松驰方法的集中率。[从作者抽象]

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简介：Inthispaper,aspiralmagneticfieldelectromagneticstirrer,whichcanseparatelyinducetwodifferentmagneticfields,isintroduced.Thecorrespondingcentralmagneticfluxcurvesofthestirrerweremeasuredbytwodif

简介：【摘要】在全面倡导构建资源节约型和环境友好型社会的背景下，医院作为一类人流量大、用水量高的公共机构，其节水问题一直是社会关注的热点话题。本文主要对医院的用水特点和广泛存在的用水问题展开分析，并针对问题提出了管理节水、技术节水和运维节水三种节水方式，旨在为医院管理者提供节水建议，助力医院的节水事业发展。

简介：Wepresentanintegratedstand-alonesoftwarepackagenamedKaKs_Calculator2.0asanupdatedversion.Itincorporates17methodsforthecalculationofnonsynonymousandsynonymoussubstitutionrates;amongthem,weaddedourmodifiedversionsofseveralwidelyusedmethodsasthegammaseriesincludingγ-NG,γ-LWL,γ-MLWL,γ-LPB,γ-MLPB,γ-YNandγ-MYN,whichhavebeendemonstratedtoperformbetterundercertainconditionsthantheiroriginalformsandarenotimplementedinthepreviousversion.Thepackageisreadilyusedfortheidentificationofpositivelyselectedsitesbasedonaslidingwindowacrossthesequencesofinterestsin5'to3'directionofprotein-codingsequences,andhaveimprovedtheoverallperformanceonsequenceanalysisforevolutionstudies.Atoolbox,includingC++andJavasourcecodeandexecutablefilesonbothWindowsandLinuxplatformstogetherwithauserinstruction,isdownloadablefromthewebsiteforacademicpurposeathttps://sourceforge.net/projects/kakscalculator2/.

简介：Imagerestorationisoftensolvedbyminimizinganenergyfunctionconsistingofadata-fidelitytermandaregularizationterm.Aregularizedconvextermcanusuallypreservetheimageedgeswellintherestoredimage.Inthispaper,weconsideraclassofconvexandedge-preservingregularizationfunctions,I.e.,multiplicativehalf-quadraticregularizations,andweusetheNewtonmethodtosolvethecorrespondinglyreducedsystemsofnonlinearequations.AteachNewtoniterate,thepreconditionedconjugategradientmethod,incorporatedwithaconstraintpreconditioner,isemployedtosolvethestructuredNewtonequationthathasasymmetricpositivedefinitecoefficientmatrix.Theigenvalueboundsofthepreconditionedmatrixaredeliberatelyderived,whichcanbeusedtoestimatetheconvergencespeedofthepreconditionedconjugategradientmethod.Weuseexperimentalresultstodemonstratethatthisnewapproachisefficient,andtheeffectofimagerestorationisr0easonablywell.

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