简介：Objective:Toinvestigatethedepressionstatusofpatientswithsexuallytransmitteddiseases(STDs).Methods:Thedepressionstatusoffifty-onehospitalizedSTDpatientswasevaluatedinarandomizedcontrolstudyusingZung'sQuantitativeTable.18healthycontrolpatientswithsimilardemographicbackgroundswererandomlychosenascontrols.Patientswithscoresaboveorequalto40wereconsideredtobesufferingfromdepression.Results:Theprevalencerateofdepressioninthepatientgroupwasobviouslyhigherthanthatofinthecontrol(X2=16.456,P<0.01).Prevalenceofdepressionwasfoundtobesignificantlyrelatedtooccupation(P<0.05).Thoughtheprevalencewasnotfoundtodiffersignificantlybetweenthosewithatreatmentcourselessthan2monthsandthosewithonelongerorequalto2months(X2=0.041,P>0.05),themeandepressionscoresoftheformergroupweresignificantlyhigherthanthoseofthelatter(P<0.01).Nosignificantdifferenceswerefoundbetweennewversusrelapsingdisease,marriedversusnon-married,maleversusfemale,ordifferingeducationalbackgrounds.

简介：Objective:ToelucidategeneralcharacteristicsofHIV/AIDSpatientsseekingcareatDitanHospitalinanattempttoguideearlydiagnosisinroutinemedicalcare.Materialsandmethods:Aretrospectiveanalysisof185HIV/AIDScasesfromJanuary1990toJune2002wascompletedusingSPSSstatisticalanalysis.Results:Maletofemaleratiowas1.8:1.Subjectsrangedinagefrom1yearoldto64yearsold.16caseswereyoungerthan20yearsoldwiththemedianageamongtheyoungersubsetat7.7years.169caseswereolderthan20yearsoldwithamedianageof36yearsold.29%ofthesubjectswerepeasants.Theremaining71%,wereofotherunspecifiedoccupations.90.8%ofindividualswereofHandescentwhile3.7%ofindividualswereofaminorityheritage.50.3%ofsubjectsweremarried;23.8%havenevermarried;8.1%weredivorced;andtheremaining17.8%wereofunknownmaritalstatus.Ofthoserepresentedinthisstudy,36.8%camefromtheHenanprovince;17.8%werefromBeijing;8.6%werefromShanxi;31.4%fromtheother20provincesofChina;and5.4%fromoutsideofChina.Modeoftransmission:40.0%(74/185)contractedHIVthroughunprotectedsexualcontact;29.2%(54/185)throughreceivingbloodorplasmatransfusions;21.1%(39/185)throughdonatingplasma;7caseswereintravenousdrugusers;7caseswereverticallytransmitted.Modeoftransmissionwasunknownin4cases.Clinicalcategories:Anincluded45cases;Bincluded85cases:Cconsistedof76cases.12casesweredeceased.Initialpresentation:39casespresentedwith_fever,coughanddiarrhea.37caseshadfeverandcoughonly-38casespresentedwithchronicdiarrhea.16caseswerediscoveredincidentallyattimeofoperation.8casespresentedwithfungalinfectionoftheoralcavityorinesophagus.ThecommonHIVassociatedsymptomsandopportunisticinfectionswere:weightlossanddiarrhea,respiratorydiseases,dermatologicdiseases,anemia,neutropenia.Diseasesofotherorgansystemswerelesscommon.Commonmisdiagnosesincluded

简介：Inordertoacknowledgethemulti-infectiondataofSTDpatientsandimprovetheprophylaxisandtherapyforSTDs，297patientsand30healthypeoplewereexaminedusingtheHSV-2plasmaantibody．TheresultsshowedthatallkindsofSTDpatientswereinfectedbyHSV-2indifierentratios．thehighestratiooccurringinsyphilispatients．

简介：Objective:TostudytheimmunologicalmechanismsofCondylomaacuminata(CA)throughinvestigatingTlymphocytesubsetlevelsandcytokineprofileintheperipheralbloodofpatientswithCondylomaAcuminata.Methods:Tricolorandbicolorimmunofluorescentstainingantibodyofcellsurfaceantigenandintracel-lularIL-2,IL-4,IL-12,IFN-γinCD4^+andCD8^+T-lymphocytesfrom20patientswithCAwereperformedandfollowedbyflowcytometry.Results:ThenumberofCD3^+T,CD4^+T-lymphocytescellsandCD4^+/CD8^+Tcellsratioweresignificantlydecreased(P<0.01)inpatientswithCAComparedtocontrols,andIL-2,IL-12,IFN-γproductioninCD4^+Tcellswasdecreased(P<0.01),IL-4andIFN-γproduc-tioninCD4^+Tcellswasnotsignificantlydifferent(P>0.05),whileIL-2andIL-12productioninCD8^+Tccellswasdecreased(P<0.01),whereasIFN-γandIL-4pro-ducinginCD4^+Tcellswereofnosignificantlydifference(P>0.05).Conclusions:TherewasanimbalanceofTlympho-cytesubsets,Th1/Th2cytokinesandTc1/Tc2intheperipheralbloodofCApatients,whichmayplayanimportantroleinthepathogenesisandprogressionofCA.

简介：Objective:ThisstudyexaminedHerpesSimplexVirus(HSV)subclinicalsheddinginthegenitaltractofpatientswithgenitalherpes(GH)ornon-gonoccalurethritis(NGU).Method:SwabswerecollectedafterexposuretorashandgenitaltractduringGHrelapseorremissiononaweeklybasisforfourtosixweeks.NGUpatientswithnegativechlamydiaandmycoplasmatestswerealsoswabbedforasimilarduration.AllswabsunderwentHSVDNAdetectionwithquantitativePCR.Result:TherewasasignificantdifferenceintherateofasymptomaticHSVsheddinginurinarytractscomparingGHandthecontrolgroupandcomparingNGUandthecontrolgroup(P<0.05).TherateofHSVsheddingwas22%,9.8%and3.3%forGH,NGUandcontrolgroupsrespectively.TherateofHSVsheddingwas21.7%(20/92)forpatientswithactiveGHand23%forthoseinremission.TheHSVpositiveratewassignificantlyhigherinthegroupwithpatientswhohadmorethansixrelapseswithinoneyearcomparedtothegroupofpatientswithlessthansixGHrelapses.Conclusion:ThereisHSVsubclinicalsheddingintheirgenitaltractduringactiveGHandremission.SubclinicalHSVsheddingismorecommoninpatientswithmorethansixGHrelapsesperyearcomparedtoGHpatientswithfewerrelapses.Approximately9.9%ofNGUpatientswithnegativechlamydia,mycoplasmatestingwasfoundtohavesubclinicalHSVinfection.

简介：Objective:Toinvestigatetheefficacyofcombina-tionofcircumcisionandmicrowaveongenitalwartsinuncircumcisedmen.Methods:Arandomized,prospectivestudyof109uncircumcisedadultmenwithgenitalwartswascon-ductedinaSTDclinicinZhanjiang,Guangdong.Onegroup(n=54)receivedmicrowavetherapyonly,whiletheothergroup(n=55)wastakenthecombinationofcircumcisionandmicrowavetherapy.Therecurrenceswereobservedattheendofmonths3,6and12,andoperativecomplicationswerealsorecorded.Results:Therewerenosignificantdifferencesinthemeanageanddurationofthediseasebetweentwogroups(P>0.05).Noseriousoperativecomplicationsweredocumented.Therecurrencerateincircumci-sionplusmicrowavegroupwasmarkedlylowerthanthatinmicrowavegroup(12.7%vs29.6%,P<0.05),whilethedifferencesinearlyandlaterecurrencesbetweentwogroupsshowednostatisticalsignificance(P>O.05).Conclusion:Circumcisioncanbesafelyperformedunderlocalanesthesiainanoutpatientsetting.Com-binationofcircumcisionandmicrowavecanproduceexcellenteffectaswellaslesstissuedamage,therefore,itmaybeidealforuncircumcisedpatientswithextensivecondvlomas.

简介：Objectives:TofindoutthelevelandfunctionsofChlamydiatrachomatisheatshockprotein(C-hsp60)antibody,anti-spermantibody(ASAb),interleukin1(IL-1),interleukin6(IL-6),interleukin8(IL-8),Tumornecrosisfactoralpha(TNF-α)andγ-interferon(IFN-γ/)inpatientswithCT-relatedinfertility.Methods:CT-DNAofcervicalsecretionswasdetectedthroughpolymerasechainreaction(PCR)andmigrationinhibitingfactor(MIF)wasemployedtomeasureIgGtitreofCTMOMPantibody.WesternblotwasusedtodeterminepresenceofC-hsp60antibodyandenzyme-linkedimmunoadsorbentassay(ELISA)measuredASAbofIgGtypeinbloodserumanddeterminethecontentofIL-1,IL-6,IL-8.TNF-u,IFN-7inuterinetubefluid.Results:68patientshadpositiveCT-DNA,amongwhich57(83.8%)hadC-hsp60antibody.Amongthe172patientswithnegativeCT-DNA,64patients(37.2%)alsohadC-hsp60Antibody.Therewasasignificantdifference(P<0.01)betweeninfertilepatientsandcontrolgrouppatientsinthepresenceofASAb.InfertilepatientswithpositiveCT-DNAhadhigherlevelsofIL-1.IL-6.IL-8,TNF-u,IFN-7inuterinetubefluidcomparedtocontrolgrouppatients(P<0.01).Conclusion:Firstly,thosepatientswithnegativeCTtestingfromcervicalsecretionscannotberuledoutforCTinfectionindeeppartsofthebody(suchasoviduct,pelvickidney).DetectionofC-hsp60AntibodymayhelptodiagnosesuchcasesofCT.Secondly,CTinfectionoftheoviductcanraiselevelsofIL-1、IL-6、IL-8、TNF-α、N-γ.ThepathogenesisofinfertilitycausedbyCTinfectioninthereproductivetractmayberelatedtocytokineproductionandinflammatoryresponsesmediatedbyC-hsp60Antibody,IL-1,IL-6,IL-8,TNF-αandIFN-γ.

简介：Object:Toinvestigatetherelationshipbetweenchlamydiatrachomatis(CT)andurogenitalinfection.Method:PositiverateofCTinpatientswithinflammationofurogenitaltractwassignificantlyhigherthanthosewithoutinflammation(P<0.05).Result:Therewasstatisticaldifferenceinthemalesnomattertheywerepatientswithinflammationofurogenitaltractornot(P>0.05),whiletherewasnostatisticaldifferenceinfemales(P>0.05).Theincidenceoftheinfectionwashighamongthoseagingfrom21-50yearsold.Conclusion:TheclinicalmanifestationsofCTinfectionwereobscure,soweshouldexamineCTinpatientswhohavenosymptoms,especiallyinfemalesandthoseofhigh-riskpopulation.

简介：Objective:Tostudythecellularimmunitystatusofpatientswithrecurrentgenitalherpes.Methods:Serumlevelsofinterlukin-2anditssolublereceptorandinterlukin-6weremeasuredbyELISAin34patientswithrecurrentgenitalherpes.Results:SerumlevelsofIL-2andIL-6weresignificantlydecreasedinpatientscomparedtohealthycontrols(P<0.01),andthelevelofsIL-2Rwassignificantlyincreasedinpatientswithrecurrentgenitalherpes(P<0.01).Therewerenosignificantdifferencesinallvariablesamongstpatientsregardingrelapsestageandremissionstage(P>0.05).Conclusion:Therewasacellularimmunedeficiencyinpatientswithrecurrentgenitalherpes.

简介：Objective:Todetecttheactivatedexpressionoftelomeraseincondylomaacuminatalesionsinlow-risk(6/11)andhigh-risk(16/18)humanpapillomavirus(HPV)infectionandexaminetheroleplayedbytelomeraseintheoccurrence,developmentandcarcinomatouschangeofcondylomaacuminata.Methods:AssayingtheexpressionoftelomeraseandthetypeofHPVindamagedskinof42CApatientsandnormalskinof30healthycontrolindividualsthroughtelomeraserepeatamplificationprotocol(TRAP)andpolymerasechainreaction(PCR).Results:Inallthenormalskincontrols,PCRforHPVwasnegativeandonly16.7%ofsampleswerepositiveforTLMAexpression;inCAlesions,HPVtestingwaspositiveinall(32caseswerelow-risk,3werehigh-risk,and7wereofmixedtype)andallwerepositiveforTLMAexpression.Conclusion:TLMAmaybeactivatedbyHPVinfection,andinturncausethehyperplasiaofepidermalcells.ItwasalsoindicatedthatHPV,especiallyhighrisktypes,canactivateTLMA.TheactivationofTLMAmayplayanimportantroleinabnormalhyperplasiaandcarcinomatouschangesinCAlesions.

简介：Objective:Toinvestigatetherolesofinterleukin-2(IL-2)andinterleukin-10(IL-10)inpathogenesisofearlysyphilis.Methods:TheserumlevelsofIL-2andIL-10in48patientswithearlysyphilisweredetectedbyABC-ELISA.Results:(1)ThelevelofIL-2inthepatientswithearlysyphiliswassignificantlyhigherthanthatinhealthycontrols,whilethatofIL-10waslower(P<0.001andP<0.001).(2)ThelevelsofIL-2andIL-10werealmostidenticalinpatientswithprimaryandsecondarysyphilis(P>0.05),aswellasbetweendif-ferentRPRtiters(P>0.05).(3)Aftertherapy,thelevelofIL-2decreasedmarkedly(P<0.05),whilethatofIL-10increase(p>0.05).(4)AsignificantcorrelationwasfoundbetweentheserumlevelsofIL-2andIL-10(r=0.5385P<0.05).Conclusions:Th1up-regulationoccursinpatientswithearlysyphilis,andplaysanactiveroleinfightingagainstTPinfection.

简介：Objective:ToconfirmwhetherMycoplasmapneumoniae(MP)arepresentinreproductivetractofSTDpatientsinChina.Methods:ApplicationofnestedPCR(nPCR)andDNAsequencingtotestsamplesofurethral/vaginalswabswithMPcultureconfirmationofseveralnPCRpositivepatients.Results:74of786STDpatientswerepositiveforMPbynPCR,witharateof9.4%.Ofthe484malepatients,10.5%werepositive,andamongthe302femalepatients,7.6%werepositive.Therewasnosignificantdifferencebetweenthem(P>0.05).Of12casesofMPpositivesamplesbynPCR,4caseswerefirstgenerationculture-positive,andoneofthempassedtothenextgenerationsuccessfully.DNAsequencingwasperformedonthenPCRproductofoneswabsampleandoneMPcultureisolation.ThedeterminedsequencewasidenticaltothetypicalMPstrain.Conclusion:InChina,MParepresentinreproductivetractofbothmaleandfemaleSTDnatients.

简介：Objective:ToexploretherelationshipbetweenquantitativeTreponemapallidumDNA(TP-DNA)PCRtestingandtheToludineRedUnheatedSerumTest(TRUST)inpatientswithsyphilisbeforeandaftertreatment,andevaluatetheclinicalvalueofquantitativeTP-DNAtestinginthediagnosisandtreatmentevaluationofsyphilis.Methods:29patientswithprimary(12cases)orsecondary(17cases)syphilis,whometthecriteriasetforthisstudywererecruitedassubjects.Allpatientsweretreatedwith2.4millionunitsbenzathinepenicillinIMweeklyfor3weeks.QuantitativetestsofTP-DNAinthepatients'plasmawereperformedusingFQ-PCRbeforeandafterthetreatment.SerologictestsincludingTRUSTandTPPAwerealsoperformed.Results:Beforethetreatment,9outof12primarysyphilispatients(75%)andallsecondarysyphilispatients(17/17)testedpositiveforTreponemapallidum(TP)byTP-DNAtesting.TheaveragequantitativetestvaluesofTP-DNAinprimaryandsecondarysyphilispatientswere(3.38±2.34)×10^4and(5.73±1.33)×10^6copies/ml,respectively.Afterthreemonthsoftreatment,1ofthe9primaryand5outof17secondarysyphilispatientswerepositiveuponTP-DNAtesting,respectively.TheaveragequantitiesofTP-DNAwere2.01×10^2copies/mlinprimaryand5.87×10^2copies/mlinsecondarysyphilispatientswithpositiveTRUSTandTP-DNAtests,and3.09×10^2copies/mlforthosewithnegativeTRUST,respectively.Afterninemonthsoftreatment,alltheprimaryandsecondarysyphilispatientswerenegativeuponTP-DNAtesting,whileallprimaryand14of17(82.35%)secondarysyphilispatientsshowednegativeTRUSTresults.Conclusion:ThattheresultsofTP-DNAtestsarenotconsistentwiththoseofTRUSTbeforeandaftertreatmentindicatesthatquantitativeTP-DNAtestingmayhavevaluableclinicalsignificanceintheearlydiagnosisandevaluationoftreatmentregimensforsyphilis.

简介：Objective:TostudythecellularimmunityfunctionofpatientswithearlysyphilisandtheeffectsonimmunemodifiersEsberitoxNorIFN.Methods:T-lymphocytesubpopulationsoftheperipheralbloodin44patientswithsyphilisand40healthycontrolswereexaminedbyflowcytometry.Results:ThenumberofCD4+cellsandtheCD4+/CD8+ratioinpatientswithsyphiliswerefoundtobesignificantlylowerthanthoseinthecontrol(P<0.01),whilethenumberofCD8+cellswashigherthanthatinthecontrol(P<0.01).TheCD4+/CD8+ratiointhosewithactivediseasewaslowerthanthatinthosewhohadbeencured(P<0.05).TheCD4+countandtheCD4+/CD8+ratiointhosetreatedwithantibioticsalone(PenicillinGorCephalosporins)werelowerthanthosetreatedwithbothantibioticsandimmunomodulators(P<0.05).Conclusions:Cellularimmunityinthepatientswithearlysyphiliswasprominentlysuppressed,andtreatmentwithimmunomodulatorsmaybehelpfulfortherecoveryofcellularimmunityofthesepatients.

简介：Objective:Toinvestigatetheprevalenceofmycoplasmainfectionsandthesensitivitytoantibioticsamongpatientswithnongonococcalurethritisorcervicitis(NGU)inChongqing.Methods:387NGUcaseswithmycoplasma-positiveresultsuponculturewereanalysedretrospectively.RESULTS:Themajorityofpatientswithmycoplasmainfectionswereinthe20-40yearoldagegroup.Nosignificantdifferencewasfoundbetweenmalesandfemales.UreaplasmaurealyticumisthemainpathogenoftheseNGUcasesandnoclearrelationshipbetweenitsconcentrationandpathogenicabilitywasnoted.Drugsensitivitywastestedagainstnineantibiotics;thesensitivityratestojosamycin,minocyclineanddoxycyclinewere94.06%,88.89%and86.82%respectively,whiletheresistanceratestolincomycin,ofloxacin,azithromycinandroxthromycinwere74.94%,42.12%,41.60%and40.31%inturn.Conclusions:Josamycin,minocyclineanddoxycyclinecouldbeusedasthefirstchoicetotreatNGUwithmycoplasmainfectionsinChongqing.ItisimportanttoselectantibioticsforNGUtreatmentwithmycoplasmainfectionsbasedontheresultsofdrugsensitivitytests.

简介：Toinvestigatetheetiologyofgenitalulcerdisease(GUD)amongpatientsattendingsexuallytransmitteddisease(STD)clinicsinGuangzhou,China.BetweenSeptember8,1998,andAugust9,assessed.ClinicaletiologyofGUDwasdependentonincludingthefollowing:darkfieldmicroscopyandserologyforTreponemapallidum(TP),swabsofgenitalulcerforsimultaneousdetectionofHSV,TP,Haemophiusducreyi(HD),Humanpapillomavirus(HPV),andserologyforHIVinfection.Twohundredthirtymenandthirty-sevenwomenwithamedianageof33.4(range16-74years)wereanalyzed.TheetiologyofGUDwassyphilis(26.59%)(71/267),genitalherpes(17.60%)(47/267),condylomataacuminata(4.87%)(13/267),candidiasis(3.37%)(10/267),andmultipeinfection(6.74%)(18/267).TheseroprevalenceofHIVwas0.07%(2/267).Noetiologywasidentifiedin50.56%(135/267).TheetiologyofGUDamongSTDpatientsinourareawasmultifactorialwithapredominanceofsyphilisandgenitalherpes.BasedonthislimiteddataobtainedatSTDclinics,HIVinfectionwasnotcommon.

简介：Objective:Toinvestigatetheeffectofactivation-in-ducedcelldeath(AICD)oncellularimmunefunctioninthecondylomaacuminatum(CA).Methods:Peripheralbloodmononuclearcells(PBMC)wereisolatedfromnormalhealthyindividuals(controlgroup)andpatientswithCA.PBMCwereculturedwithPHA-Pfor48hinvitro.ApoptosisofthePBMCwasdetectedbyflowcytometry.Supernatantcytokines(IL-2andIL-10)wereassayedbyELISA.Results:TherateofPBMCapoptosisinbothCAgroupandcontrolgroupinfreshPBMCwasverylowandsimilarinbothgroups(P>0.05).TherateofPBMCapoptosiswithintheCAgroupwasnoticeablyincreasedcomparedtothatofthecontrol(P<0.001)afterPBMCwereculturedfor48h.ThelevelofIL-2wassignificantlylowerintheCAgroupthaninthecontrolgroup(P<0.001),ThelevelofIL-10wassignificantlyhigherintheCAgroupcomparedtothecontrolgroup(P<0.001).Conclusion:StudyresultsindicatethatAICDmayaffectcellularmediatedimmunefunctionandplayanimportantroleinthepathogenesisofCA.

简介：Summary:RiskfactorsforgenitourinaryChlamydiatrachomatis(Ct)infectionwereinvestigatedusinganSTDrelativeriskfactorquestionnaireamong176patientsandtheirregularsexualpartners.Twenty-fourindependentvariableswereselectedforanalysis,nineofwhichwereconfirmedasfactorsassociatedwithC.trachomatis.Fouroftheninefactorsweresignificantlycorrelatedtoinfectionusingamultifactorialunconditionallogisticregressionmodelwhichincluded:levelofeducation(oddsratios[ORs]:2.144forbelowjuniormiddleschool),numberofsexualpartners(ORs:4.503for≥5),numberofregularpartner'ssexualpartner(ORs:16.333for≥5),STDhistoryofregularpartner(ORs:18.417fortheirSTDhistory).Thesedatademonstratethatregularpartner'ssexualbehaviorsarealsoanimportantriskfactorforCtinfectionamong,STDclinicclients.

简介：Objective:TostudytheexpressionofFasandBcl-2proteinsonTlymphocytesubsetsintheperipheralbloodofrelapsingpatientswithcondylomaacuminatum(CA)andhealthycontrols.Methods:Flowcytometry(permeabizationandstainingprocedurewithconjugatedantibodies)wasused.Results:WeobservedthattheexpressionofFasproteinonCD4^+TlymphocytesubsetofCApatientswassignificantlyhigherthanthatofhealthycontrols(P<0.01).Conclusions:IncreasedexpressionofFasproteinonCD4^+Tlymphocytesubsetmaybeacauseofde-creasedpercentageofCD4^+Tlymphocytesubset.ThisinducestheincreasedratioofCD4^+/CD8^+.

简介：Objective:Tostudytheexpressionlevelsofplatelet-derivedgrowthfactor(PDGF)andgranulocytecolony-stimulatingfactor(G-CSF)inperipheralbloodandtheirroleinthepathogenesisofCondylomaacuminatum(CA).Methods:Seraweretakenfrom70patientswithCondylomaacuminatumandcomparedwith35healthycontrols.PDGFandG-CSFinserumwerequantitatedusingadualantibodysandwichenzyme-linkedimmunoabsorbentassay(ELISA).Results:SerumconcentrationsofPDGFandG-CSFweresignificantlyincreasedinpatientswithCondylomaacuminatum(CA)comparedtocontrols(P<0.001andP<0.005respectively).SerumlevelsofPDGFandG-CSFcorrelatedwithclinicalseverityofCA,butnosignificantdifferencewasobservedbetweendifferentdurationofdiseasegroups.AsignificantpositivecorrelationwasnoticedbetweenneutrophilcountandG-CSFlevels(γ=0.38,P<0.001),andtheneutrophilcountshowednosignificantcorrelationwithPDGF.Conclusion:TheresultsindicatedthatincreasedexpressionofPDGFandG-CSFinperipheralbloodmightbeinvolvedinpathogenesisofCA.