简介：ThirtyWistarratswererandomlyandevenlydividedintocontrolgroup,cerebralischemiagroupandischemia+electroacupuncture(EA)group.Thebilateralcommoncarotidarterieswereoccludedtoinduceacutecerebralischemia.Nitricoxide(NO)andendothelin(ET)contentsinthecerebraltissuesandbloodweremeasuredundernormalcondition,immediatelyafterischemiaandfollowingEA.ResultsshowedthatafteracutecerebralischemiaNOandETcontentsinthecerebraltissuesincreasedsignificantly(P<0.01)whileserumETincreasedandserumNOloweredobviously(P<0.05).FollowingEAofBaihui(GV20)andDazhui(GV14),bothNOandETincerebraltissuesandserumturnedtonormalbasically.ItshowedthatEAcouldprotectthecerebraltissuesfrominjuryinducedbyischemia,NOandETmightparticipateinthemodulationprocessofEA.

简介：Objective:Toinvestigatetheunderlyingneurobiologicalmechanismoftheprotectiveeffectofelectroacupuncture(EA)duringcerebralischemia-reperfusion(CI-R).Methods:Inthefirstpartofthestudy,15SDratswereevenlyrandomizedintocontrolgroup,CI-R-48hmodelgroupandCI-R-48h+EAgroup.ThecorticalapoptosisandexpressionofBcl-2andBaxproteinsineachgroupweredetectedbyflowcytometer(FCM).Inthesecondpartofthestudy,75SDratswereevenlyrandomizedintocontrol,CI-R-3min,CI-R-3min+EA,CI-R-48handCI-R-48h+EAgroups.Corticalnorepinephrine(NE)concentrationwasdetectedbyfluorescencespectrometer.CI-Rmodelwasestablishedbyocclusionofthebilateralcommoncarotidarteriesandreperfusion.EA(4～16Hz,1～3V)wasappliedafterreperfusionrespectively.Results:Inthefirstpartofthisstudy,resultsindicatedthatthenumberoftheapoptoticneuronsandtheapoptosisrateofCI-R-48hgroupweresignificantlyhigherthanthoseofcontrolgroup;whilecomparisonbetweenCI-R-48h+EAandCI-R-48hgroupsshowedthatthenumberoftheapoptoticneuronsandtheapoptosisrateoftheformergroupweresignificantlylowerthanthoseofthelatergroup(P<0.05).Incomparisonwithcontrolgroup,afterCI-48h,Baxexpressionwasup-regulatedsignificantlyandBcl-2down-regulatedmarkedly(P<0.05).ComparisonbetweenCI-R-48handCI-R-48h+EAgroupindicatedthatBaxexpressionofthelatergroupwassignificantlylowerthanthatoftheformergroup,whileBcl-2expressionofCI-R-48h+EAgroupwassignificantlyhigherthanthatofCI-R-48hgroup(P<0.05),suggestingthatEAcouldreverseCIinducedreactionsofthesetwoindexes.Inthesecondpartofthestudy,incomparisonwithcontrolgroup,NEconcentrationincerebralcortexofCI-R-3mingroupincreasedsignificantly(P<0.05);whileNEcontentofCI-R-3min+EAgroupwassignificantlylowerthanthatofCI-R-3mingroup(P<0.05).NosignificantdifferencewasfoundbetweenCI-R-3mingroupandcontrolgroupincorticalNEl

简介：Objective:Toobservetheeffectofacupunctureonimagesinautismchildren.Methods:Atotalof27casesofautismchildrenweresubjectedintothisstudy.ByusingaSPECT,thecerebralimageswerecollectedbeforeandafteracupuncturetreatmentandanalyzedaccordingtotherecommendedmethodsinforassessingthestateofbloodflow,radioactivityquantitydistributionandradioactivitycountinbilateralhemispheres.'JIN'sthree-needling'wasemployed.Theacupuncturetreatmentwasgivenonceeveryotherday,with4monthsbeingatherapeuticcourseandanintervalofonemonthbetweentwocourses.Results:Afteracupuncturetreatment,ofthe22cases,20hadremarkableimprovementand2hadimprovementincerebralbloodflow,withthetotaleffectiverateofimprovingcerebralbloodflowbeing90.8%.Beforethetreatmentthereweresignificantdifferencesbetweentheleftandrightcerebrum(P<0.001),andbetweentheleftandrightfrontallobesinradioactiveareas(P<0.01);however,aftertreatment,nodifferenceswerefoundbetweenthem(P>0.05).Aftertreatment,theradioactivitycountinthewholebraindecreasedsignificantlyincomparisonwiththatofpretreatment(P<0.01).Itindicatestheimprovementofcerebralbloodflowandcellularmetabolismafterthetreatment.Conclusion:Acupumctureconsignificantlyimprovecerebralbloodflowinautismchildren.

简介：Objectives:Toexploretheeffectofacupunctureoncerebralinfarctioninratsandtotryprovidingsomeexperimentalparametersforclinicalpractice.Methods:27healthyWistarratswererandomlydividedintopseudo-operation(n=10),model(n=8)andacupuncture(n=9)groups.Neuro-functionaldefectscoring,apoptosisofsinglebrainsliceandthenumberofbcl-2immuno-reaction(IR)-positiveneuronsinCA1areaofthehippocampuswereusedastheindexestoinvestigatethepossiblemechanismsofacupunctureof'NieSanZhen'(needlingthreeacupointsinthetemporalregion)and'SiShenZhen'(needlingfouracupointsintheocciputregion)intreatingratswithcerebralinfarction.Results:Thereexistedsignificantdifferencesbetweenacupuncturegroupandmodelgroupinimprovingneurologicfunctionalactivities,inhibitingapoptosisofthebraincellsandincreasingbcl-2IR-positiveneuronsinthehippocampalCA1area(P＜0.01).Conclusion:Acupuncturetherapycanimprovecerebralinfarctionintheratbysuppressingapoptosisandup-regulationofthebcl-2IR-positiveneuronexpression.

简介：Objective:Toobservethetherapeuticeffectofoculo-acupuncturetherapyinthetreatmentofcerebralinfarctioninducedhemiplegia.Methods:58casesofstrokepatientswererandomlydividedintotreatmentgroup(n=30)andcontrolgroup(n=28).Intreatmentgroup,onthebasisofmedicationplusrehabilitationtreatment,patientsvoluntarilyacceptedoculo-acupuncturetherapy(acupunctureofUpper-JiaoAreaandLower-JiaoArea).Incontrolgroup,patientsonlyacceptedmedicationandrehabilitationtreatment.ThetherapeuticeffectwasevaluatedwithBrunstrom's6-stagesAssessingMethod.Results:After24sessionsoftreatment,theratiosofthediseasedlimbsreachingstageⅥandmoreinfunctionalactivityinthepatientsoftreatmentgroupincreasedfrom16.7%(upper-limb)and20.0%(lower-limb)beforetreatmentto70.0%and90.0%respectively;whileincontrolgroup,thoseratiosoftheupperandlowerlimbsincreasedfrom10.7%and28.6%beforetreatmentto39.2%and60.7%separately.Therewasasignificantdifferencebetweentwogroupsinthetherapeuticeffect(P＜0.05).Conclusion:Oculo-acupunctureplusmedicationissuperiortosimplemedicationtreatmentinimprovingfunctionalactivityofthehemiplegiclimbs.

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简介：Inthepresentpaper,theauthorsreviewrecentadvancesinclinicalandexperimentalstudiesonacupuncturetreatmentofcerebralhemorrhage(CH).Regardingclinicalstudies,theresuscitation-inducingneedlingmaneuver,andmainpointsofShuigou(水沟GV26),Baihui(百会GV20)andscalp-pointsMotorArea(MS6),SensoryArea(MS7),etc.areofteninvolved.Concerningexperimentalstudies,theunderlyingmechanismsofacupunctureofGV-26+'Neiguan'(内关PC6),GV-20,GV-26+GV-20,etc.inimprovingacuteCHareintroduced.Inaword,acupuncturetherapyworkswellinimprovingclinicalsymptomsandsignsofCHpatients,andacupuncturestimulationinducedameiliorationofcerebralbloodflow,favorablemodulationofsomebioactivesubstancesasexcitatoryandinhibitoryaminoacids,endothelin,CGRP,heatshockprotein70,etc.andneuro-endocrine-immunenetworkmaycontributetotheeffectofacupunctureonCH.Inaddition,acupuncturecombinedwithmedicineandearlierapplicationofacupuncturetherapyintheacutestageofCHarerecommendedinclinicalpractice.

简介：Objective:Toobservetheeffectofelectroacupuncture(EA)onsynapticstructureofhippocampalnervefeltsandsynaptophysin(SYN)expressioninratswithcerebralischernicinjury.Methods:SixtyWistarratswererandomizedintoshah-operationgroup,cerebralischemia(CI)groupandEAgroup,eachofwhichwasfurtherdividedinto1week(W)and5Wsubgroups.Clinjurymodelwasestablishedbyocclusionofthebilateralcomrnoncarotidarter-ies.“Baihui”(百会GV20),“Dazhui”(大椎GV14),“Renzhong”(人中GV26)and“Guenyuan”(关元CV4)werepuncturedandstimulatedelectrically.Thebraintissuesectionscontaininghippocampusregionwerestainedwithirnrrunohistochernicaltechniqueandobservedunderlightmicroscopeandtransmissionelectronicmicroscope.Results:AfterCI,theischemicinjuryasdegenerationofthepresynapsecompositions,decreaseofthesynapticnumeraldensity,andlowexpressionofSYNwereobservedinhippocampalCA1area.Bythe5^thweekafterCI,theneonatalsynapsesofCIandEAgroupsappeared,andSYNexpressionwasupregulated.InEAgroup,therecoveryofthenumeraldensityofsynapseswasespeciallynoticeable,being93.8%ofthatofsham-operationgroupandsignificantlyhigherthanthatinCIgroup(P<0.01).Comparedwithsham-operationgroup,thecalibratedopticaldensity(COD)valuesofSYNincreasedto70%inCIgroup,and93.3%inEAgroup,andCODvalueinEAgroupwassignificantlyhigherthanthatinCIgroup(P<0.01).Conclusion,EAcanfunctioninpromotingsynapticregenerationandenhancingandperfectingtheactionsofthereconstructedsynapsesinhippocampalCA1areainCIrats.

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简介：Insufficientbloodsupplyofcerebralarteriesisacommonlyseenandfrequentlyoccurreddiseaseamongthemiddle-agedandsenilepeople.Inrecentyears,theauthorofthispapertreated68casesofinsufficientbloodsupplyofcerebralarteriesbyacupuncturewithsatisfactoryresults.Itisreportedasfollows.

简介：Objective:Toobservetheeffectofacupunctureoncerebralglucosemetabolisminstrokepatients.Methods:Changesofcerebralglucosemetabolismbeforeandafteracupuncturestimulationwereobservedinsixcasesofstrokepatientsbyusingpositronemissiontomography(PET)scanner.Electroacupuncture(EA,4Hz,continuouswavesanddurationof20min)wasappliedtoBaihui(百会GV20)andrightQubin(曲鬓GB7).18Fluorinedeoxyglucose(18FDG),adeveloper(radioactiveformofglucose)forshowingthelevelsofthebrainfunctionalactivitywasgiventothepatientsintravenously.SPMsoftwarewasusedtodealwiththedataofeachpixelpointbyunilateralt-test(Ts:P=0.05),then,theregionsshowingincrease/decreaseoftheglucosemetabolismwereobtained.Results:Afteracupuncturestimulation,significantincreaseofglucosemetabolismwasfoundtobeinthefirstsomaticmotorcorticalregion(MI),supplementarymotorarea(SMA),premotorarea(PMC),andthesuperiorparietallobule(LPs)onthehealthysideofthebrain;whilethedecreaseofglucosemetabolismfoundinMI,PMCandLPsonthefocusside.Inadditiontothecerebralregionsrelatedtothemotorfunction,changesofglucosemetabolismwerealsofoundintheparietallobuleandbasalganglionarea,centralparietalgyrus,superiorparietalgyrus,putamen,cerebellum,etc..Conclusion:AcupunctureofQubin(GB7)andBaihui(GV20)canactivatemotor-relatedcerebralstructuresinthebilateralcerebralhemisphereandinduceexcitementreactionofthepotentiallycorrelativemotorareasoastocompensateorassisttheinjuredmotorareatoplayaroleinimprovingmotorfunctioninstrokepatients.

简介：Inthepresentstudy,theinvolvementofL-arginine(L-Arg)NOoniheprotectiveactionofelectroacupuncture(EA)oncerebralischemicinjurywasobservedinacuteischemia-reperfusion(IR)ratmodelbytakingregionalcerebralbloodflow(r-CBF),cerebralwatercontent(CWC),andbloodnitricoxide(NO)contentsasindexes.Resultsshowedthat1)EAcouldcauser-CBFandserumNOcontenttoincreaseandCWCtolower,suggestinganprotectiveactionofEAonIRcerebralinjury;2)intravenousinjectionofL-ArgalsohadanprotectiveeffectoncerebralIRcerebralinjury,whileL-NNAhadnothiseffect;and3)pre-treatmentwithL-ArgmightstrengthentheeffectofEAfurther,whilepretreatmentwithL-NNAcouldweakenitseffect.ItindicatesthatL-Arg-NOmaybeinvolvedintheeffectofEAinprotectingthebrainfromischemicinjury.

简介：Objective:Tostudythemechanismofelectroacupuncture(EA)ofscalp-pointsforregulatingabnormaldischargesofneuronsindifferentregionsaroundthecerebralhemorrhagefocusbyusingneuro-electrophysiologicalmethods.Methods:80Wistarrats(anesthetizedwith20%urethane1g/kg,I.p.)wererandomlydividedintonormal,saline,modelandEAgroups,with20casesineachgroup.Cerebralhemorrhagemodelwasestablishedbyintracerebralinjectionoftherat'sownarterialbloodsample(40uL).Inratsofsalinegroup,thesamevolumeofsalinewasgivenforintracerebralinjection.ExtracellularelectricalactivityofneuronsofthecaudatenucleusandparafascicularnucleusandTailfiliformneedlesandstimulatedelectricallywithstimulatingparametersofstrengthof1V,frequencyof15Hzanddurationof15min.Results:Comparedwithnormalgroup,TFLvaluesofmodelgroupandEAgroupincreasedsignificantly(P<0.01);andcomparedwithmodelgroup,thoseofEAgroupdecreasedsignificantly(P<0.01),suggestingthatthepainthresholdincreasedsignificantlyincerebralhemorrhageratswhileafteracupuncturestimulation,itloweredstrikingly.Comparedwithnormalandsalinegroups,thelatencyvaluesofthepainexcitementandinhibitoryresponsesofthecellulardischargesofthecaudateandparafascicularnucleiinmodelandEAgroupsincreasedsignificantly(P<0.05～0.01),whileafterEA,itrecoveredapparently(P<0.01),showinganapparentregulativeeffectofEAontheabnormalchangesofdischargesofneuronsaroundthecerebralhemorrhagefocus.Conclusion:Scalp-acupuncturepossessesanapparentregulatoryeffectontheabnormalelectricalactivityofneuronsaroundthecerebralhemorrhagefocuswhichmayfavortheearlyrecoveryoffunctionalactivityofneuronsnearthefocustissues.

简介：Objective:Toobservethetherapeuticeffectofelectroacupuncture(EA)onplasmaangiotensin(Ang*.Ⅱ),aldosterone(ALD)andatrialnatriureticpolypeptide(ANP)contentsinexperimentalcerebralinfarctionrabbitsforanalyzingtheunderlyingmechanismofacupunctureinamelioratingbloodsupplyofthebraintissue.Methods:Atotalof80rabbitswererandomizedintocontrol(n=8),pseudo-operation(n=24),model(n=24)andEA(n=24)groups.Cerebralinfarctionmodelwasestablishedbyinfusionofself-thrombusintothecarotidartery.EA(1mA,2Hz)wasappliedto'Baihui'(百会GV20)and'Shuigou'(水沟GV26)for30min,onceevery12hours.PlasmaAng-II,ALDandANPcontentsweredetectedwithradioimmunoassaymethod.Inthelater3groups,bloodsamplesweretakenat6h,24hand48haftercerebralischemia.Results:Comparedwithcontrolandpseudo-operationgroups,Ang-IIandALDcontentsofmodelgroupat6h,24hand48haftercerebralischemiaincreasedsignificantlywhileplasmaANPofthe3time-coursesofmodelgroupdecreasedconsiderably(P<0.01).Incomparisonwithmodelgroup,resultsshowedthatAng-IIandALDcontentsofEAgroupdecreasedsignificantlywhereasANPlevelofEAgroupincreasedstrikingly(P<0.01).Conclusion:ElectroacupuncturehastheeffectsofraisingplasmaANPlevelandloweringplasmaAng-IIandALDincerebralinfarctionrabbits.

简介：Objective：Toobservetheeffectofelectro-scalpacupunctureonglucosemetabolismofcerebralregionsinvolvingmentalactivityinhealthypeople.Methods:Atotalof6casesofvolunteerhealthysubjects(3malesand3females)ranginginagefrom22to36yearsweresubjectedtothisstudy.Changesofcerebralglucosemetabolismbeforeandafterelectro-scalpacupuncturewereobservedbyusingpositronemissiontomography(PET)andsemi-quantifyinganalysismethod.Electro-scalpacupuncturestimulation(50Hz,2rnA)ofMiddleLineofVertex(Dingzhongxian,顶中线,MS5),MiddleLineofForehead(Ezhongxian,额中线,MS1)andbilateralLateralLine1ofForehead(Epangyixian,额旁一线,MS2)wasadministeredfor30minutes.Thencerebralregionsofinterest(ROIs)werechosenandtheiraverageglucosemetabolismlevels(radioactivityof18fluorinedeoxyglucose)wereanalyzed.Results：Afteradministrationofelectro-scalpacupuncture,theglucosemetabolismlevelsinbilateralfrontallobesandbilateralcaudatenuclei,leftcingulategyrusandrightcerebellumincreasedsignificantlyincomparisonwiththoseofprestimulation(P<0.05).Conclusion:Electro-scalpacupunctureofMS1,MS2andMS5canincreasetheglucosemetabolismofcertaincerebralregionsinvolvingmentalactivityinhealthysubjects.

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