简介:Objective:Toinvestigatetheculturemethodofskin-derivedprecursors(SKPs)andtoexploreanewcellsourceforcelltransplantationofcentralnervoussystem.Methods:Cellsfromskinsofjuvenileandadultmicewereisolatedandculturedinserum-freemedium.Amechanicalmethodwaschosentopassagethesecellsandtheywereidemifiedbytheimmunocytochemistryassay.Results:SKPscouldbeisolatedfromadultandneonatalskins.Theycouldbemaintainedinvitroforlongperiodswithstableproliferation,andexpandedasundifferentiatedcellsincultureformorethan12passages.About50%ofSKPsexpressednestinandmajorityofthesecellsexpressedfibronectinwhentheywereplatedonpolyornithineandlaminincoatedplates.About5%cellsshowedneuronaldifferentiationandexpressedneurofilament-M(NF-M)andNSEwhenSKPswereplatedinserun-containingmedium,andthesecellscouldalsodifferentiateintoadipocytesandfibroblast-likecells.Conclusions:Thedatasupportthehypothesisthatadultskincontainsstemcellscapableofdifferentiatingintoneurons,adipocytes,andfibroblast-likecells.TheymayrepresentanalternativeautologousstemcellsourceforCNScelltransplantation.
简介:讨论联合外部fixator和皮肤拍动调换的效果在上的目的暴露并且不属于工会的骨头。开的骨折在1998年3月的经期期间在我们的医院里对待到2008年6月的感染的不属于工会、暴露的骨头列在后面的12个盒子的数据被分析的方法。有10个男病人,2个女病人,其变老在19-52年之间并且平均28年。有10胫骨的破裂和2大腿骨的破裂。疾病的功课与19个月的吝啬的时期持续了12-39月。所有盒子被联合外部fixator和皮肤拍动调换对待。结果主要愈合在10种情况中被完成并且推迟在胫骨由于软织物缺点和因此本地的拍动调换在被暴露的2种情况中愈合被执行。所有12个盒子与8个月的平均时间在操作以后在6-12月以内有多骨的联合。他们被跟随在上面为1-3,年和所有破裂愈合了在上面与好功能和没有感染复发。联合外部fixator和皮肤拍动调换治疗在对待上看了最佳的效果的结论感染了,暴露并且不属于工会的骨头
简介:客观:在单个教材扩大(矿泉)和高密度的oligonucleotideDNA数组的帮助下学习在earliergestational皮肤和老鼠的以后的gestational皮肤之间的基因表示的差别理解scarlesshealing的分子的机制。方法:全部的RNA从疤更少(E15)的胎儿的老鼠皮肤被孤立怀孕期(学期=21.5天)的形成andscar(E18)时期。从更早的gestational皮肤(EGS)和以后的gestational皮肤(LGS)的RNA相对地两个都被抄录到cDNAs,然后为由矿泉方法准备杂交探针用荧光灯的dCTP的加入标记。Themixed探针当时是到包含了5的oligonucleotideDNA数组的hybridized代表5705老鼠基因的705根探针。在高度紧的洗以后,这些DNA数组被扫描因为显示差别的荧光灯的信号表示了在2组skin.Results之间的基因:在5705老鼠基因之中,与差别有53基因(0.93%)在EGS和LGS组,之间的表示层次27基因,包括成纤维细胞生长因素2(FGF2)并且follistatinwere起来调整(0.47%)并且26基因是下面调整的(0.46%)在胎儿的皮肤在scarlessperiod对形成疤的时期期间。在EGS的FGF2和follistatin的更高的表情被RT-PCR方法也比那些inLGS揭示。结论:高密度的oligonucleotideDNA数组为调查微分基因表示在提供了一个强大的工具更早并且latergestational胎儿的皮肤。这种技术验证胎儿的疤更少愈合的机制是很复杂化,许多基因表情的变化与胎儿的疤被联系更少的愈合。
简介:Objective: Tocomparethedynamicchangesofinterleukin-1(IL-1),interleukin-6(IL-6),andtumornecrosisfactor(TNF)inintermingledskingraftwiththoseinothertypesofskingraftsinrats. Methods: A10%-15%third-degreeburnwascreatedin180Spregue-Dawley(SD)rats.Afterremovingthescar,skingraftswereperformedontheopenwoundsimmediatelywithautoskin(aus,n=54),alloskin(als,n=54)andintermingledskin(n=36).Thatistosay,intheintermingledskingraft,abigpieceofalloskin(mals)wasgraftedfirst,and3dayslater,smallpiecesofautoskin(maus)wereembeddedinthealloskin.Therest36ratsweretakenasthecontrols.AndthebiologicalactivitiesofIL-1,IL-6andTNFingraftsheetsineachgroupweredetectedafterskingraft. Results: ThelevelsofIL-1,IL-6andTNFintheausgroupdecreasedsteadilyaftertheirinitialelevations,whereasinthealsgrouptheyincreasedsignificantlyandkeptonthepeaklevelinthelaterphases.Intheintermingledgroup,thereappearedalowestIL-1levelinthemalsandahighestoneinthemaussimultaneouslyat7(4)days(Thenumberoutofparenthesisisthedaysaftertransplantingwithalloskinsheets,andthenumberinparenthesisisthedaysafterembeddingautoskinsheetsintheintermingledskingraft.Similarlyhereinafter.)afterskingraft(P<0.01),andthehighlevelinthemausabruptlydecreasedat14(11)daysafterskingraft.Atexactlythesamephaseonday7(4),aprominentpeakedIL-6inthemalsoccurred.Inthelaterphases,thelevelsofTNFremainedrelativelylowbothinthemalsandinthemaus.Fromday7(4)on,eachcytokinefluctuationinthemalssynchronizedwiththatinthemaus.Thelongertheposttransplantationperiodlasted,themorethepositivecytokinecorrelatedbetweenthemalsandthemaus. Conclusions: ThelowlevelsofIL-1andTNFmaybeimportantfactorstolightentheintensityoflocalrejectionintheintermingledskingraft.Thetempo