简介:目的建立高表达缓激肽受体(B2R)的大鼠胶质瘤模型,为研究缓激肽选择性开放血脑屏障的机制及解决目前临床应用中存在的问题提供必要的模型.方法①大鼠B2R的真核细胞的表达和其载体(prB2R)侵染C6胶质瘤细胞株;②Real-timeRT-PCR测定B2R的转录;③WesternBlot法测定B2R的表达水平.结果①大鼠胶质瘤细胞株C6高表达B2R;②Real-timeRT-PCR测定C6-B2R1和C6-B2R2克隆的B2R分别比C6对照高7.6和6.9倍;③C6-B2R1和C6-B2R2克隆的蛋白表达水平高于C6对照克隆的3.8和3.78倍.移植C6-B2R1肿瘤1周后的B2R表达水平高于C6对照肿瘤的3.6倍.结论高表达B2R的大鼠胶质瘤模型已被成功建立.
简介:目的:研究脑肿瘤手术前后血清S100B蛋白水平的改变,并分析其与患者临床资料的相关性,评估血清S100B对术后脑损伤的反映能力。方法30例胶质瘤、28例脑膜瘤和15例听神经瘤患者于手术前(入院时)、手术后第1d、第3d、第7d分别采集血清;同时记录患者手术时长、肿瘤WHO级别、肿瘤体积、脑水肿体积、KPS等临床资料。设正常对照组33例,采集单次血清。用双抗体夹心法ELISA检测血清S100B含量。将手术前后血清S100B水平进行重复测量方差分析;将血清S100B水平与手术时长、肿瘤体积等临床资料进行相关性分析。结果术前血清S100B水平在各脑肿瘤组之间的差异无统计学意义(P>0.05),胶质瘤组、脑膜瘤组则高于正常对照组(均P<0.05)。术后第1d、第3d血清S100B含量无明显改变(P>0.05),术后第7d时高于手术前水平(P<0.05),这种趋势在3个脑肿瘤组之间并无差别。在胶质瘤组中,术后第3、7d血清S100B含量与术后脑水肿体积呈正相关(均P<0.05);术后第1d、第3d血清S100B含量与胶质瘤病理级别呈正相关(均P<0.05)。在听神经瘤组中,手术前、术后第3d血清S100B含量与听神经瘤肿瘤体积呈负相关(均P<0.05),术后第7d血清S100B水平与手术时长呈正相关(P<0.05)。脑膜瘤组内未见任何相关性。结论血清S100B对脑肿瘤切除术后的脑损伤反映较差,其含量升高可能与损伤后神经修复活动有关。血清S100B含量与胶质瘤的病理级别、术后脑水肿程度有一定的相关性,与听神经瘤体积及手术时长存在相关性。脑肿瘤术前血清S100B升高可能反映了肿瘤对脑实质的压迫损伤。
简介:目的研究红藻氨酸(KA)致痫大鼠海马S100B、降钙素基因相关肽(CGRP)的表达及病理改变。方法雄性SD大鼠按照完全随机数字表法分成对照组(8只)和模型组(40只),模型组再根据处死时间分为造模后6h、12h、24h、72h、1周5个亚组,每组8只。模型组采用KA建立颞叶癫痫动物模型,对照组用等体积生理盐水代替KA注射。模型组造模后6h、12h、24h、72h、1周.对照组注射后24h取大鼠海马组织行Niss1染色、Timm染色和免疫组化染色,观察S100B、CGRP蛋白的表达情况以及海马神经元和胶质细胞的病理变化。结果Nissl染色结果显示,模型组大鼠1周后CA3区出现大量固缩的坏死神经元,胞体萎缩,尼氏体消失。Timm染色结果显示,模型组大鼠1周后CA3区始层出现条带状分布的棕色颗粒,齿状回内分子层亦可见少量棕色颗粒。免疫组化染色结果显示,模型组大鼠海马CGRP蛋白大量表达,72h时达到高峰,同时伴随大量神经元丧失及胶质细胞增生。结论KA致痫大鼠出现S100B、CGRP蛋白高表达,尼氏体消失,苔藓纤维发芽等一系列病理学改变,推测S100B、CGRP蛋白参与了癫痫发生。
简介:BACKGROUND:Ithasbeenshownthatginsenoside,theeffectivecomponentofginseng,canenhanceexpressionofcholineacetyltransferase,aswellasbrain-derivedneurotrophicfactor(BDNF)anditsreceptortyrosinekinaseB(TrkB),incholinergicneuronsofthebasalforebrain.OBJECTIVE:ToqualitativelyandquantitativelyverifytheinfluenceofginsenosideonexpressionofBDNFanditsreceptor,TrkB,inthemedialseptumofagedrats,andtoprovideamolecularbasisforclinicalapplication.DESIGN,TIMEANDSETTING:Acontraststudy,whichwasperformedintheDepartmentofAnatomy,ChinaMedicalUniversity,andtheDepartmentofAnatomy,ShenyangMedicalCollegebetweenDecember2005andMay2007.MATERIALS:Thirty-five,healthy,female,SpragueDawleyratswereselectedforthisstudy.Ginsenoside(81%purity)wasprovidedbyJilinJi’anWantaiChineseMedicineFactory;anti-BDNFantibody,anti-TrkBantibody,andtheirkitswereprovidedbyWuhanBosterCompany.METHODS:Atotalof35ratsweredividedintothreegroups:young(fourmonthsold),aging(26monthsold),andginsenoside.Ratsintheginsenosidegroupwereadministeredginsenoside(25mg/kg/d)between17monthsand26months.MAINOUTCOMEMEASURES:ImmunohistochemistryandinsituhybridizationwereusedtomeasureexpressionofBDNFandTrkBinthemedialseptumofagedrats,andthedetectedresultswereexpressedasgrayvalues.RESULTS:①Qualitativedetection:usingmicroscopy,degenerativeneuronswerevisibleinthemedialseptumintheaginggroup.However,neuronalmorphologyintheginsenosidegroupwassimilartoneuronsintheyounggroup.②Quantitativedetection:themeangrayvalueofBDNF-positiveandTrkB-positiveproductsintheaginggroupweresignificantlyhigherthanintheyounggroup(t=3.346,4.169,P<0.01);however,themeangrayvalueintheginsenosidegroupwassignificantlylowerthanintheaginggroup(t=2.432,2.651,P<0.01).CONCLUSION:GinsenosidecanincreaseexpressionofBDNFandTrkBinth
简介:目的探讨大剂量高压氧治疗方案对永久陆大脑中动脉闭塞大鼠的疗效以及高压氧对大鼠梗死部位周围脑组织核因子κB(nuclearfactor-κB,NF-κB)影响。方法制备雄性Sprague--Dawley大鼠永久陛大脑中动脉闭塞模型,随机分为高压氧组和对照组,每组32X,另设立伪手术组。使用Garcia神经行为学评分方法分别在术后24h、5d对大鼠进行神经行为学评分;应用2,3,5一三苯基氯化四氮唑(2,5,5-trjphenyltetrazolfumchlorid,TTC)方法对脑组织进行染色,观察24h、5d时大鼠脑组织梗死容积;取梗死部位周围脑组织,采用凝胶电泳迁移实验(electrophoreticmobilityshiftassay,EMSA)方法检测术后24h、5d时的NF-κB脱氧核糖核酸(deoxyribonucleicacid,DNA)结合活性。比较三组间上述指标的差异。结果术后24h高压氧组神经行为学评分高于对照组[(13.33±1.53)坩(10.33±0.58),P〈0.001]。术后24h高压氧组梗死容积小于对照组[(139.75±33.59)坩(203.02±57.66),P=0.008]。术后5d高压氧组梗死部位周围脑组织NF-κB活性低于对照组[(16.01±4.56)坩(50.28±9.15),P=0.035]。结论大剂量高压氧治疗方案在脑梗死后24h内具有脑保护作用。大剂量高压氧可降低大鼠梗死部位周围脑组织NF-κBDNA结合活性。
简介:目的:评价高分辨率3D-FIESTA+c成像及图像处理技术显示脑神经及其病变的价值。方法采用3D-FIESTA+c对20例健康志愿者和20例临床疑是因血管等原因压迫相应脑神经具有临床症状的患者进行扫描及图像后处理。由2名神经放射学医师根据20名健康志愿者480支脑神经显示的清晰程度分为清晰、较清晰、不清晰3个等级,清晰和较清晰定义为显示,不清晰定义为未显示;临床病例中,脑神经与血管关系分为无接触、接触、压迫。结果12对脑神经显示率分别为:嗅神经84.3%,视神经100%,动眼神经100%,滑车神经43.8%,三叉神经100%,外展神经100%,面神经100%,前庭蜗神经100%,舌咽神经、迷走神经及副神经复合体100%,舌下神经47.1%。20例脑神经症状患者,16例确诊为脑神经与周围血管接触或压迫,且均被临床治疗证实。结论高分辨3D-FIESTA+c成像与图像后处理技术相结合可显示脑神经及其病变,能准确定位血管走向及其与脑神经的关系,为临床医生提供准确、全面的影像学资料。
简介:目的研究GOLPH3在胶质瘤组织中的表达及其临床意义.方法选取山东大学齐鲁医院和聊城市脑科医院神经外科自2008年7月至2009年12月间手术切除并经病理证实的人脑胶质瘤标本76例,其中Ⅰ级13例,Ⅱ27级例,Ⅲ级25例,胶质母细胞瘤11例.另取9例因脑创伤行内减压术患者的正常脑组织标本作为对照.应用RT-PCR、Westernblotting分别检测各标本中GOLPH3mRNA和GOLPH3蛋白的表达.结果RT-PCR与Westernblotting检测结果显示正常脑组织中GOLPH3mRNA和GOLPH3蛋白不表达,不同级别胶质瘤组织GOLPH3mRNA、GOLPH3蛋白的阳性表达率差异无统计学意义(P>0.05),而其表达值差异有统计学意义(P<0.05),且随着肿瘤病理级别的增高,胶质瘤组织中GOLPH3mRNA、GOLPH3蛋白表达值增高,差异有统计学意义(P<0.05).相关性检验显示GOLPH3蛋白的表达值与肿瘤的病理分级呈正相关(rs=0.961,P=0.000).结论GOLPH3在不同级别的胶质瘤组织中均有表达,其表达值与肿瘤的病理分级呈正相关,可能是胶质瘤发生发展的重要机制之一.
简介:目的探讨B超和吲哚菁绿血管造影(ICGA)在脑浅表动静脉畸形(AVMs)手术中的应用价值.方法回顾性分析自2009年1月至12月北京天坛医院神经外科血管组联合应用B超和ICGA辅助切除的16例脑浅表AVMs患者临床资料,同时分析2种术中辅助技术对AVMs定位、边界确定及血管类型鉴别的作用.结果术中联合应用ICGA和B超能有效帮助定位AVMs,确定其边界,帮助辨认供血动脉和引流静脉.16例脑浅表AVMs患者共行开颅手术16次,均全切病灶,手术后经DSA证实AVMs无残留.结论脑浅表AVMs手术中联合应用ICGA和B超能有效帮助准确切除病灶,判断有无畸形残留,具有较高的临床应用价值.
简介:Anumberofpreviousstudiesofacupunctureacupointspecificityhaveusedshamacupoints,shamacupunctureormeridianacupointsatagreatdistancefromeachotherascontrolsinfunctionalMRI(fMRI)experiments.However,fewstudieshavecompareddifferentmeridianacupointswithinthesamesegment,whichareassociatedwithsimilarlyintenseneedlesensations.WeperformedfMRIon12healthyyoungvolunteersandobserveddifferencesinbrainactivationelicitedbyacupunctureoftheTaixi(KI3)andQiuxu(GB40)acupoints.AcupuncturewasappliedattheTaixiandQiuxuacupoints,usingamultiple-blockfMRIdesignwiththreeblocks,involvingthreealternationsofrestingandtaskphases.Afterscanning,needlesensationwasassessed.ThebehavioralresultsrevealedthatthesubjectiveneedlesensationwassimilarbetweentheTaixiandQiuxuacupoints.ThefMRIresultsrevealedthatacupunctureattherightTaixiacupointactivatedtherightsuperiortemporalgyrus(BA22),leftmiddlefrontalgyrus(BA46)andinferiorfrontalgyrus(BA45),bilateralparietallobepostcentralgyrus(BA2),rightparietallobe(BA3),andleftparietallobe(BA40).AcupunctureattherightQiuxuacupointactivatedtheleftsuperiortemporalgyrus(BA42),rightparietallobepostcentralgyrus(BA40,BA43),rightinferiorfrontalgyrus(BA47),bilateralsuperiortemporalgyrus(BA22),andrightinsulaBA13.TheseresultssuggestthattherightTaixiandQiuxuacupointsactivateddifferentbrainareas.
简介:BACKGROUND:Nerveallograftrejectionisanunavoidableproblemfornerveallografts.Traumaticperipheralnerveinjuriesarecommonlyreconstructedusingautogenousnervegrafts.However,thisformofreconstructionislimitedbyinsufficientautologousnervesforlargegaprepairsandbymorbidityatthenervedonorsite.OBJECTIVE:ToexaminesciaticnerveregenerationandimmunetolerancereactionafterintragastricadministrationofultravioletB-irradiated(UVB)donorsplenocytes.DESIGN,TIMEANDSETTING:Acompleterandomizedgroupingdesignandcontrolledexperiment.TheexperimentswereconductedintheDepartmentofOrthopedics,theFirstAffiliatedHospitaltoShanxiMedicalUniversity,China,betweenMarchandOctober2007.MATERIALS:FourteenadultmaleSDratsandfourteenmaleWistarrats,weighing250–300g,wererandomlymatchedasdonorsandacceptors.AfurthersevenmaleSDrats(weight250–300g,age12–16weeks)wereusedfornerveisografts.ImmunepreparationsandtheEpicsXLflowcytometerwerepurchasedfromB-DCompany,USA.Acomputer-assistedelectromyographmachinewasprovidedbyKeypointP,DantelCompany,Denmark.METHODS:SplenocytesfromWistarratswereisolated,purified,andcultured,andthenirradiatedwithultravioletB.Inthefirstcontrolgroup(Group1),theSDratsreceivedasyngeneicSDnerveisograft.Inthesecondcontrolgroup(Group2),theSDratsreceivedanerveallograftfromWistarratswithoutpretreatment.Intheoral-tolerancetreatedgroup(Group3),theSDrecipientratswereinoculatedwith2.5×107LewisUVB-irradiateddonorsplenocytecellsbyintestinaltractadministrationatsevendaysbeforetransplantation.MAINOUTCOMEMEASURES:(1)Therecentendandthemiddleanddistalendofthetransplantednervewerecutat8and12weeksafteroperation.RecoveryofnerveregenerationwasmeasuredwithHEstainingusingthetotalnumber,density,anddiameterofthenervefibers.(2)ThelevelofCD25+Tlymphocytesinperipheralbloodwasdetec
简介:WithintheCNSnuclearfactor-kappaB(NF-κB)transcriptionfactorsareinvolvedinawiderangeoffunctionsbothinhomeostasisandinpathology.Overtheyears,ourandothergroupsproducedavastarrayofinformationonthecomplexinvolvementofNF-κBproteinsindifferentaspectsofpostnatalneurogenesisInparticular,severalextracellularsignalsandmembranereceptorshavebeenidentifiedasbeingabletoaffectneuralprogenitorcells(NPC)andtheirprogenyviaNF-κBactivation.AcrucialroleintheregulationofneuronalfatespecificationinadulthippocampalNPCisplayedbytheNF-κBp50subunit.NF-κBp50KOmicedisplayaremarkablereductioninadulthippocampalneurogenesiswhichcorrelateswithaselectivedefectinhippocampal-dependentshort-termmemory.MoreoverabsenceofNF-κBp50canprofoundlyaffecttheinvitroproneurogenicresponseofadulthippocampalNPC(ahNPC)toseveralendogenoussignalsanddrugs.HereinwebrieflyreviewthecurrentknowledgeonthepivotalroleofNF-κBp50intheregulationofadulthippocampalneurogenesis.InadditionwediscussmorerecentdatathatfurtherextendtherelevanceofNF-κBp50tonovelastroglia-derivedsignalswhichcaninfluenceneuronalspecificationofahNPCandtoastrocyte-NPCcross-talk.
简介:Inthepresentstudy,weconstructedalentivirus,FIV-CMV-GFP-miR-7-3,containingthemicroRNA-7-3geneandthegreenfluorescentproteingene,andusedittotransfecthumangliomaU251cells.Fluorescencemicroscopyshowedthat80%ofU251cellsexpressedgreenfluorescence.Real-timereversetranscriptionPCRshowedthatmicroRNA-7-3RNAexpressioninU251cellswassignificantlyincreased.ProliferationwasslowedintransfectedU251cells,andmostcellswereintheG1phaseofthecellcycle.Inaddition,theexpressionoftheserine/threonineproteinkinase2wasdecreased.ResultssuggestedthattransfectionwithalentiviruscarryingmicroRNA-7-3caneffectivelysuppressepidermalgrowthfactorreceptorpathwayactivityinU251cells,arrestcellcycletransitionfromG1phasetoSphaseandinhibitgliomacellgrowth.