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简介：目的观察单纯疱疹病毒2型(HSV-2)对单一核细胞产生肿瘤坏死因子α(TNF-α)和白介素-6(IL-6)的影响,探讨单一核细胞在HSV-2感染中的作用。方法用HSV-2(333株)感染单一核细胞,于病毒感染1,3,5,7日收集细胞培养上清液,用ELISA法检测TNF-a、IL-6的含量。结果HSV-2感染后1日,单一核细胞分泌TNF-α水平显著降低。HSV-2感染后1,3日,单一核细胞分泌IL-6水平显著降低,以后,病毒感染单一核细胞分泌TNF-α、IL-6水平逐渐增高,到第7日达到对照组水平。结论HSV-2能抑制单一核细胞分泌TNF-α和IL-6。而这两种细胞因子在HSV-2致病和机体免疫中起重要作用。

简介：皮肤病中西医结合研究已经取得较多进展.

简介：包茎、包皮过长是男科常见的一种疾病，我科采用CO2激光包皮套环切除术治疗包茎、包皮过长患者96例，取得了较满意的疗效，现报告如下。

简介：Objectiwe:InordertodetecttheroleofmonocytesinHSV-2infection,westudiedtheeffectofherpessim-plexVirus-2infectionontheproductionoftumorne-crosisfactor(TNF-α),interleukin-6(IL-6)secretedbymonocytes.Methods:MonocyteswereinfectedbyHSV-2(333Strain).Culturesupernatantswerecollectedat1,3,5,7dayspost-infection.ThelevelsofTNF-α,IL-6weremeasuredbyenzyme-linkedimmunosorbentas-say(ELISA).Results:ThelevelsofTNF-αsecretionbymono-cytessignificantlydecreasedonfirstdaypost-infection.ThelevelsofIL-6significantlydecreasedonfirstandthirddayspost-infection,andthengradu-allyincreasedtothecontrolonseventhdaypost-infection.Conclusions:TNF-αandIL-6productionbymono-cyteswasinhibitedduringHSV-2infection.Thepro-ductionofcytokinesmayplayanimportantroleinherpessimplexviurs-2pathogenicityandimmunity.

简介：目的研究复发性尖锐湿疣(condylomaacuminatum,CA)患者与正常对照者T细胞亚群Fas及Bcl-2蛋白的表达。方法采用流式细胞仪,结合抗体渗透染色法进行检测。结果CA患者外周血CD4T淋巴细胞亚群Fas蛋白的表达较正常人显著增加。结论CD4~+T细胞百分率下降与其高表达Fas蛋白有关。CD4~+/CD8~+比值下降主要是由于CD4~+细胞百分率下降所致。

简介：Objective:ThisstudyanalyzedtheTlymphocytesandThl/Th2typecytokineprofileshiftintheperipheralbloodofpatientswithrecurrentgenitalherpes(RGH).Methods:Immunofluorescentstainingofcellsurfaceantigenandintracellularcytokines(IL-2,IL-4,IL-12,IFN-r)inperipheralbloodfrom20RGHpatientsand10controlswereanalyzedusingflowcytometrictechniques.Results:RGHpatientshadsignificantlylowerlevelsofCD3^+Tcells,CD4^+TcellsandCD4^+T/CD8^+Tcellsratiocomparedtocontrollevels(P<0.001),andIL-2-producing,IFN-r-producingandIL-12-producingTcellswereincreasedinRGHpatients(CD4^+T:P<0.001,CD8^+T:P<0.05respectively),whereasIL-4-producingTcellswereincreasedinRGHpatientscomparedtocontrols(CD4+T:P<0.05;CDS^+T:P<0.001respectively).Conclusions:RGHpatientshaveTlymphocytesubsetvariationsandThl/Th2cytokinechanges.TheincreaseinTh2cellsThl/Th2imbalancemayhaveimportantimplicationsforRGHpathogenesis.

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简介：Objective:TostudytheexpressionofFasandBcl-2proteinsonTlymphocytesubsetsintheperipheralbloodofrelapsingpatientswithcondylomaacuminatum(CA)andhealthycontrols.Methods:Flowcytometry(permeabizationandstainingprocedurewithconjugatedantibodies)wasused.Results:WeobservedthattheexpressionofFasproteinonCD4^+TlymphocytesubsetofCApatientswassignificantlyhigherthanthatofhealthycontrols(P<0.01).Conclusions:IncreasedexpressionofFasproteinonCD4^+Tlymphocytesubsetmaybeacauseofde-creasedpercentageofCD4^+Tlymphocytesubset.ThisinducestheincreasedratioofCD4^+/CD8^+.

简介：Objective:Tostudytheexpressionofactivatedepi-dermalgrowthfactorreceptor(EGFR)andtranscrip-tionfactorE2F(E2F)inCondylomaAccuminata(CA)patients.Methods:ImmunofluorescenttechniqueswereusedtoinvestigatetheexpressionofactivatedEGFRandE2FinCApatients.Results:TheexpressionofactivatedEGFRonthemembraneofepithelialcellsinCAlesionswassig-nificantlygreatercomparedtoexpressionleversinthecontrolgroup(P<0.01).Moreover,theco-expres-sionofactivatedEGFRandE2Fwassignificantlyin-creasedcomparedtothecontrolgroup(P<0.01).Conclusion:Ourobservationssuggestthatthein-creaseinactivatedEGFRexpressionmaystimulatehyperplasiainCApatientsthroughtheactivationoftranscriptionfactorE2F.

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简介：Objective:TostudytheeffectoftraditionalChinesemedicineantiviralcapsulesinthetreatmentofgenitalherpes.Methods:Usingfemaleguineapiggenitalherpesastheanimalmodel,thisstudyusedoraladministrationoftwoformulationsofantiviralcapsules(AC)andobservedtheeffectonvaginalHSV-2titersandvulvarsymptoms.CellcultureswerealsousedtoexaminethedirectinactivationofHSV-2bytheantiviralcapsulesandthesuppressionofHSV-2viathreedrugadministrationmethods.Results:Therewasnosignificantdifferenceofmeanvaginalvirustitersbetweentheantiviralcapsulegroupsandthatofthepositiveacyclovir(ACV)control(P>0.05).Meanvulvarsymptomscoresofthetwoantiviralcapsulegroupswerealsosignificantlylowerthanthatofthesalinenegativecontrolgroupondays2,3,5,7and8(P<0.05)andsimilartothatoftheACVcontrol(P>0.05).CellcultureshowedtheminimuminhibitoryconcentrationsofantiviralcapsulesNo.1andNo.2were0.390625mg/mland1.5625mg/ml,respectively.Conclusion:ThetraditionalChinesemedicineantiviralcapsuleshadsuppressiveeffectsonHSV-2inbothanimalmodelGHandinvitrocellculture.

简介：Rowell综合征（RS）是红斑狼疮（LE）的一种罕见表现，伴发多形红斑样皮损，血清中抗核抗体、抗La（SS-B）／抗Ro（SS-A）抗体、类风湿因子（RF）阳性。以往文献中有作者认为此综合征是皮肤红斑狼疮的一种特殊变异。作者报道1例64岁女性LE患者和1例51岁女性LE和Sjogren综合征（SS）患者，均伴发多形红斑样皮损，并对红斑狼疮与多形红斑并存的情况进行了讨论。

简介：高免疫球蛋白E综合征（HIES）是一种罕见的免疫缺陷性疾病，表现为血清IgE水平升高、皮肤湿疹、反复皮肤感染和特征性的肌肉骨骼表现。作者报道2例在本院就诊的患者并回顾当前文献。患者1是一18个月大的非裔美国男孩，伴有复发性葡萄球菌冷脓肿、肺炎和菌血症。该患者皮肤严重湿疹化，最终并发疱疹性湿疹。在选用敏感抗生素环孢素A（5mg／kg）短期治疗全身性感染后，皮炎改善，随后改为长期口服甲氧苄啶一磺胺甲恶唑。患者2是一15岁高加索男孩，长期患有HIES。该患者从3岁开始即维持每周3次注射1干扰素和每月静脉注射免疫球蛋白，并预防性应用抗生素和小剂量氟康唑。

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