简介:Smallubiquitin-likemodifier(SUMO)-conjugatingenzymesareinvolvedinpost-translationalregulatoryprocessesineukaryotes,includingtheconjugationofSUMOpeptidestoproteinsubstrate(SUMOylation).SUMOylationplaysanimportantroleinimprovingplanttolerancetoabioticstresssuchassalt,drought,heatandcold.Herein,wereportedtheisolationofOsSCE1(LOC_Os10g39120)geneencodingaSUMO-conjugatingenzymefromrice(Oryzasativacv.Nipponbare)anditsfunctionalvalidationinresponsetodroughtstress.TheE2enzyme,OsSCE1,isoneofthreekeyenzymesinvolvedintheconjugationofSUMOtoitstargetproteins.ActivatedSUMOistransferredtothecysteineofanE2enzymeandthentothetargetlysineresidueofthesubstrate,withorwithoutthehelpofanE3SUMOligase.ExpressionofOsSCE1wasstronglyinducedbypolyethyleneglycol6000(PEG6000)treatment,whichsuggestedOsSCE1maybeinvolvedinthedroughtstressresponse.OverexpressionofOsSCE1(OsSCE1-OX)inNipponbarereducedthetolerancetodroughtstress.Conversely,thedroughttolerancewasslightlyimprovedbytheknockdownofOsSCE1(OsSCE1-KD).TheseresultswerefurthersupportedbymeasurementofprolinecontentinOsSCE1-OXandOsSCE1-KDtransgeniclinesunderinduceddroughtstress,whichshowedOsSCE1-KDtransgeniclinesaccumulatedhigherprolinecontentthanthewildtype,whereasOsSCE1-OXlinehadlowerprolinecontentthanthewildtype.ThesefindingssuggestedOsSCE1mayplayaroleasanegativeregulatorinresponsetodroughtstressinrice.
简介:本文在籼、粳稻细胞质背景下研究了Rf-1位点上PCR标记M45461的遗传分离比例,结果显示M45461的遗传分离与提供雄配子的杂合体的细胞质背景有关。粳稻细胞质不影响M45461的遗传分离比例,而籼稻细胞质会导致M45461极显著偏向籼稻或具有籼稻遗传成分较重的亲本。在籼、粳稻细胞质背景下,杂合体的花粉育性分别为半不育和正常可育,而小穗育性均正常。说明M45461的偏分离与雄配子选择有关,而与雌配子关系不大。该结果还揭示粳稻细胞质可以与籼稻细胞核和谐共存,籼稻细胞质则与粳稻细胞核存在不谐和的遗传互作。这一结论可以为籼粳分化的研究提供一些参考,也可为籼粳杂交父母本选择提供参考。
简介:Theclosed-jarincubationmethodiswidelyusedtoestimatethemineralizationofsoilorganicC.TherearetwoCpools(i.e.,organicandinorganicC)incalcareoussoil.ToevaluatetheeffectofadditionalcarbonatesonCO2emissionfromcalcareoussoilduringclosed-jarincubation,threeincubationexperimentswereconductedbyaddingdifferenttypes(CaCO3andMgCO3)andamountsofcarbonatetothesoil.TheadditionofcarbonatessignificantlyincreasedCO2emissionfromthesoil;theincreaserangedfrom12.0%intheCaCO3amendedsoilto460%intheMgCO3amendedsoilduringa100-dincubation.CumulativeCO2productionattheendoftheincubationwasthreetimesgreaterintheMgCO3amendedsoilcomparedtotheCaCO3amendedone.TheCO2emissionincreasedwiththeamountofCaCO3addedtothesoil.Incontrast,CO2emissiondecreasedastheamountofMgCO3addedtothesoilincreased.Ourresultsconfirmedthattheclosed-jarincubationmethodcouldleadtoanoverestimateoforganicCmineralizationincalcareoussoils.BecauseofitseffectonsoilpHandthedissolutionofcarbonates,HgCl2shouldnotbeusedtosterilizecalcareoussoiliftheexperimentincludesthemeasurementofsoilCO2production.
简介:为了阐明提高谷氨酰胺合成酶(GS)活性是否可以提高烟草的耐盐能力,本试验以烟草栽培品种K326为对照,研究TaGS1/TaGS2转基因烟草抗盐能力及其机制。结果表明,盐胁迫条件下,过表达TaGS1/TaGS2烟草与对照相比,根系较发达,烟株生物量较高,TaGS2转基因烟草尤为显著;转基因烟草的叶绿素含量、气孔导度、净光合速率、GS活性、可溶蛋白含量等碳氮代谢功能均显著优于野生型K326;且转基因株系脯氨酸含量及含水量较高,MDA含量较低,叶片渗透调节能力和质膜保护能力比K326强。研究表明TaGS1/TaGS2的过表达提高了烟草的耐盐能力,其高GS活性是维持碳氮代谢抵抗盐胁迫的生理基础。
简介:测试了木薯加工废弃物生物发酵转化有机肥产品ZH-1、ZH-2在白菜薹上的应用效果设有机肥ZH-1(处理1)、有机肥ZH-2(处理2),并以高浓度复合肥(CK-1)、不施肥(CK-2)作为对照处理,通过田间栽培试验研究施用有机肥ZH-1和ZH-2对白菜薹经济性状、品质、产量及经济效益的影响。结果表明,3个施肥处理均起到促进白菜薹生长的效果,各指标测定结果显著或极显著优于不施肥处理(CK-2)。在施肥处理中,有机肥ZH-1和ZH-2处理白菜薹单位产量比同等养分条件下施用高浓度复合肥(CK-1)分别低6.92%和4.44%,但2个有机肥处理白菜薹的品质更好,商品质量更高,经济效益比施用高浓度复合肥(CK-1)分别提高32.32%和38.55%,其中有机肥ZH-2的综合施用效果尤为突出。因此,新型有机肥料产品ZH-1和ZH-2值得在白菜薹生产上应用推广。
简介:以萨米脱和奇好樱桃果实为试材,研究了常温(20℃)贮藏条件下不同处理方式(CK、ClO2、1-MCP、1-MCP+ClO2)对果实采后生理和品质的影响。结果表明,与CK相比,ClO2处理可明显降低樱桃果实的腐烂率,1-MCP处理各项指标无明显变化规律,1-MCP+ClO2处理可明显降低萨米脱(4~12d)、奇好(2~12d)樱桃果实的呼吸强度,抑制果实硬度、VC、可溶性固形物和可滴定酸含量的下降,延缓果实衰老,延长贮藏保鲜期,但对乙醇含量无明显影响。1-MCP+ClO2处理的萨米脱樱桃常温贮藏6d,奇好樱桃常温贮藏12d,可保持较低的腐烂率和失重率,且具有良好的风味和口感。