简介：SolublemalewormantigenofSchistosomajaponicurn(Sj)wasinvestigatedfordevelopmentofnewvaccinecandidate.SDS--PAGEandWesternblottingwereperformedtocomparethedifferencebetweensolubleantigensfromwormsofdifferentsex.MicevaccinationwiththetestingpurifiedproteinwasfollowedbySjcereariaechallengetodetecttheprotectiveeffectagainstSj.Sixteenbandswereseenforthesolublemalewormantigenand12forthefemaleworm.Inaddition,adistinctbandof44.6kDafromthemalewormantigenwasobserved,anditsantigenicitywasdemonstratedbyWesternblotting.This44.6kDaproteincouldinducesignificantwormandeggreductionrateinmice(39.31%,41.98%,P<0.001).Inthisstudya44.6kDaproteinwasisolatedandpartiallycharacterized.Itsantigenicity,immunogenicityandthepartialimmuneprotectionsuggestitspotentialvaccinecandidteagainstSj.

简介：ToselecttheimmunogenicshortpeptidemimicsofmalewormoriginofSchistosomajaponicum(Sj)andtoexploretheirprotectioneffectagainstschistosomiasisinmice,therandomphagedisplaypeptidehbraryof12-merwasscreenedwithIgGtosolublemalewormantigenofSj,andthespecificpositiveclonesselectedthroughthreeroundsofscreeningsweredetectedbyDot-ELISA,andtheninjectedsubcutaneouslyintomiceforvaccinationandprotectionassessmentagainstSj.Itwasfoundthat18randomlypickedphagedisplayedclonesallshoweddefiniteantigenicitywithvariousintensities.Thepooledphagesdisplayedclonescouldinduceproductionofspecificantibodiesandcause31.72%ofwormreductionrateand51.54%ofeggreductionrateinmice,revealingasignificantdifference(P<0.001)incomparisonwiththoseofthecontrols.ItconcludesthattheshortpeptidemimicsofmalewormoriginofSjobtainedbyaffinityscreeningphagedisplayptidelibrarycanelicitpartialprotectionagainstthispathogen.