简介：由把OsRacDcDNA用作探查屏蔽光周期的genomic图书馆敏感妖怪男性无菌的米饭行Nongken58S，包含编码OsRacD的区域的2kb倡导者and396bp的积极克隆被获得。与reversePCR从正常米饭变化Nongken58(Nongken58N)克隆的OsRacD的倡导者相比，相同是99.8%，并且不同核苷酸在在倡导者的预言的反应元素外面，建议在在长天的条件下面的米饭变化Nongken58S和Nongken58N之间的富饶是没在OsRacD在上游的规定序列的结构归因于差别，但是到基因的发展规定微分表示。

简介：Perivascular上皮状的房间肿瘤(PEComas)是稀罕的间充质的瘤的一个家庭。PEComas，镇静上皮状并且锭子房间，有细胞的一样和immunohistochemical特征但是在不同内脏、软的织物地点被发现。这里，我们报导组织学并且PEComa的一个案例的immunohistochemical特征在一个男病人的骨盆的内脏的腹膜限制了。病人与激进的外科被对待，并且好并且没有肿瘤复发，在后续上访问。

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简介：理解photoperiod/thermo-sensitive的男绝育机制遗传因子的男性无菌[P(T)公克]在米饭的线，光周期或温度的遗传上的研究进步在米饭的敏感遗传因子的男绝育被考察。一个新想法被建议解释P(T)公克米饭的绝育机制。富饶转变从对无菌肥沃是有光周期或温度的主要效果的无菌的基因的合作规定的结果敏感基因，然而并非在P(T)公克米饭的所谓的pgms基因。次要效果的基因，在绝育上展出积聚的效果，是为绝育转变的批评温度的重要因素。无菌的线保持越多次要效果的基因，越多降低绝育转变的批评温度。如果所有次要效果的基因是同型结合的，绝育转变的批评温度将是不变的。为引起P(T)公克米饭的策略也被建议。为绝育转变的批评光周期和温度的选择索引应该被放根据品种的类型和生态的区域。强加选择压力是为为绝育与更低的批评温度引起P(T)公克米饭的一种关键技术，并且改进整个特点和联合能力的全面表演为引起P(T)公克米饭线是重要的。

简介：试图学习在里面vivo男白化体老鼠上的Tabernaemontanadivaricata叶摘录的反煽动性的活动。方法水的煎和甲醇叶摘录为他们减少巴豆的能力被测试在在热门申请以后的鼠标耳朵的导致油的浮肿。甲醇叶摘录dose-dependently禁止了巴豆在老鼠的导致油的耳朵浮肿(ID50<500?g

简介：Di-n-butylphthalate(DBP)是有潜力影响男复制的内分泌破坏的化学药品。然而，低剂量的DBP的繁殖效果仍然不是众所周知的，特别在分子的水平。在现在的学习，pubertal男性Sprague-Dawley老鼠是在大量剂量的口头上地管理的DBP(0.1，1.0，10，100和500 ；为30天的mgkg−1天−1)。选择结束点包括了繁殖机关重量，阴囊的组织病理学说和浆液神经质的层次。另外，proteomic分析被执行识别是在低剂量由于暴露表示到DBP的差别的蛋白质(0.1，1.0和10 ；mgkg−1天−1)。有毒的效果在高剂量的组被观察，包括睾丸和epididymides的异常开发，生精的小管，精子发生的损失和浆液荷尔蒙的反常层次的严重萎缩。有DBP的低剂量的处理似乎施加‘；浆液荷尔蒙上的刺激effect'。老鼠睾丸的Proteomics分析证明20差别表示了蛋白质。(SOD1)在这些蛋白质，在HnRNPA2/B1的表示的改变，vimentin和superoxidedismutase之中，1被西方的污点和immunohistochemistry进一步证实。一起拿，我们断定DBP的高剂量导致了阴囊的毒性，并且DBP的低剂量在在Sertoli和Leydig房间的数字和功能象变化一样涉及精子发生的蛋白质的表示导致了变化，尽管没有明显的词法变化出现了。这些差别的鉴定表示了蛋白质关于在雄的老鼠复制上位于DBP的效果下面的机制提供重要信息。

简介：Thisstudywasconductedtodevelopandcharacterizeanovelcytoplasmicmalesterile(CMS)sourcewhichwasidentifiedfromDongxiangwildrice(Oryzarufipogon)bycrossingDongxiangwildriceasfemalewithZhongzao35,anindicainbredvariety,asmaleandcontinuousbackcrossingwithZhongzao35.ObservationunderopticalmicroscopemanifestedthatthisnovelCMSbelongedtotypicalabortiontypewithlesspollencomparedwithwildabortivetypecytoplasm(CMS-WA).SequentialplantingshowedthatthisnovelCMShascompleteandstablemalesterility.Testcrossexperimentshowedthatallthe24testedmaterialsincludingmaintainerandrestorerlinesofCMS-WAandHongliantypecytoplasm(CMS-HL)andotherindicainbredvarietiesarethemaintainerswithcompletemaintainingability,suggestingthatthisnovelCMShasfertilityrestorationtotallydifferentfromCMS-WAandCMS-HLandbelongstoanoveltypeofCMS.Sofar,weonlydiscoveredauniquefertilityrestorationsourceforthisnovelCMS.InheritanceanalysisshowedthatthefertilityrestorationofthisCMSwasgovernedbythreepairsofindependentdominantgenes.ProspectforapplicationofthisnovelCMSsysteminhybridricebreedingwasalsodiscussed.

简介：植物温度(Tp)和它与米饭殖民地和灌溉水的小气候的关系用thermo敏感的遗传因子的男性被学习无菌(TGMS)米饭线，Pei'ai64S。在温度的每日的变化的重要差别在150厘米(TA)的高度在Tpand空气温度之间被检测。从8:00到20:00，Tp比TA，而是他们在到下7:00的21:00期间是类似的的低。最大的Tp比最大的TA一个小时早发生了，尽管他们两个都在6:00到达了最小。Tp波动不到TA。在一样的高度，在6:0013:00期间，Tp比空气温度(Ta)高，并且Tp比Ta一个小时早到达了最大值。在在阳光充足的白天的剩余的时间期间，到近或甚至有点的Tpwas比Ta降低。在阴暗白天，比在整个天内的Ta高的Tpwas，和两个同时最大化。另外，Tpwas在夜里在白天由太阳的放射，云量和风速度，并且由灌溉水调整了。现在的学习显示了那29.6甠?潴?的TA?慥獲τ

简介：AbstractObjective:There are few reports of live births from heterotopic transplantation of frozen-thawed ovarian tissue. The purpose of this study is to assess the follicular development in the frozen-thawed ovarian tissues following heterotopic transplantation in both female and male bodies.Methods:Cluster of differentiation 1 (CD1) mice (6-8 weeks) were used in this study as ovarian tissue donors and foster mothers for embryo transfer. Sperm from CD1 male mice were used for insemination by intracytoplasmic sperm injection (ICSI). Nude severe combined immunodeficiency mice (8 weeks) were employed as recipients of ovarian tissue transplantation. The frozen-thawed ovarian tissues were transplanted to 4 sites on each mouse, female and male, subcutaneously. After 3 months, both female and male mice were injected with 5.0 IU gonadotropins intraperitoneally. Post 48 hours of injection, the mouse was killed for ovarian transplant collection. Only fully grown oocytes with contacted cumulus cells (cumulus-oocyte complexes) were then selected for maturation in vitro. In vitro matured oocytes were inseminated with fresh sperm by ICSI, and the developed blastocysts were frozen using the vitrification method and stored until embryo transfer. After thawing, the thawed blastocysts were incubated for at least 2 hours before the transfer. The foster mice mothers mated with vasectomised male 3 days previously. Live birth was monitored at 19 days after transfer, and the resulted offspring was raised for fertility test.Results:The relatively high recovery rates of the transplanted ovarian tissues were collected in both frozen-thawed and fresh ovarian tissue transplants from both female and male bodies. The fully grown immature oocytes became mature in vitro and the fertilized zygotes developed to blastocyst stage. There are no differences between frozen-thawed and fresh ovarian transplants in term of oocyte quality and embryo development to blastocyst rates. Nineteen-day post-transfer, 3 foster mothers from the frozen-thawed ovarian tissue transplant group delivered 13 pups and the 4 foster mothers of the fresh ovarian tissue transplant group delivered 12 live pups. The produced offspring were normal in appearance and grew healthy and fertile.Conclusions:Our results attest that the follicles can survive and develop in the frozen-thawed ovarian tissues following the subcutaneous transplant to adult male mouse’s body regardless of basal endocrinal environment. Those fully grown oocytes can produce healthy and fertile offspring which will provide the possibility for further mechanistic understanding of endocrinology of folliculogenesis.

简介：在影响男繁殖的道的开发的基因的单个核苷酸多型性(SNP)与男泌尿生殖器的畸形被联系了。然而，没有研究测试了在象阴肛部的距离(AGD)那样的SNP和中间的显型之间的关系，anoscrotal距离(ASD)和阴茎宽度(PW)。我们测试了在影响男生殖器的开发的八基因的24普通SNP是否为未来家庭从学习在106个健康男婴儿与中间的显型被联系。我们从口腔的油迹和线性回归模型使用了DNA为covariates与调整估计在阴肛部的大小和SNP遗传型之间的关系。我们发现rs2077647G等位基因，在雌激素受体alpha(ESR1)的编码区域定位了，与更短的AGD被联系(P=0.02；−；7.3公里，95%；信心间隔(CI)：−；11.6到−；3.1)，并且rs10475T等位基因，在3′定位了；激活抄写因素3的untranslated区域(ATF3)，与更短的ASD被联系(−；4.3 ；公里，95%；CI：−；7.2到−；1.4)，尽管这结果不是重要的(P=0.07)在为多重比较控制以后。我们没观察在测试的PW和SNP之间的协会。为在调整在男生殖器的开发期间发信号的雌激素的基因的二SNP的次要的等位基因与AGD和ASD被联系，尽管协会的意义是边缘的。我们的调查结果suggest ；那AGD和ASD被可继承的factors 影响；在知道与象hypospadias和cryptorchidism那样的坦率的男生殖器的畸形被联系的基因。

简介：瞄准：在老鼠男性检验不可分的膜蛋白质2b(Itm2b)的表示和规定繁殖纸巾在性成熟期间并且在不同治疗由下面在situ杂交。方法：睾丸，epididymis，和管推迟ens在天1-70上被收集在性成熟期间检验Itm2b表示。为了推进，检验Itm2b的规定，成年老鼠经历了外科的阉割和cryptorchidism。乙烯暗淡乙烷sulfonate和busulfan治疗被执行在Leydig房间和细菌房间的破坏以后测试Itm2b的规定。结果：在睾丸，Itm2b表示中等在天1-10上在生精的绳索的广告钠区域被检测，并且在天在spermatogonia在底层检测了20和30。Itm2b水平显著地从到白天70的白天20在Leydig房间被增加。在epididymis和管推迟ens，Itm2b从出生不满一月的婴儿被检测到逐渐地根据性成熟增加的成年人，和信号。Itm2b表示是显著地击倒在epididymis和管调整了推迟阉割的老鼠的ens，并且当阉割的老鼠与睾丸激素被对待时，强烈刺激了。Cryptorchidism在睾丸和头epididymis导致了Itm2b表示的重要衰落。在epididymis和管的Itm2b表示推迟在Leydig房间被乙烯破坏以后，ens显著地被减少暗淡乙烷sulfonate。Busulfan处理没在epididymis或管在Itm2b表示生产明显的变化推迟ens。结论：我们的数据建议那个Itm2b表达式起来在老鼠男性复制由睾丸激素和力量玩调整了一个角色。

简介：与他们的男、女的父母一起的十F1联合被采用在flowering和早谷物充满阶段期间评估他们的热忍耐。米饭植物被使遭到加热应力(39??????僚吗？？

简介：因为输精管切除术的不可避免的复杂并发症，这研究被承担与一个微型外科的方法与没有解剖刀输精管切除术(NSV)相比植入进管腔的一台nonobstructiveintravas设备(IVD)估计男消毒的功效和安全。IVD被分成二种类型：而，IVD-B让一条尾巴使用了修理到管deferens(固定翅膀)IVD--A不做。multicenter未来的使随机化的控制临床的审判在中国被进行。学习由寻求输精管切除术的1459个男志愿者组成随机被分到IVD--一(n=487)，IVD-B(n=485)或NSV(n=487)组并且经历了操作。包括的后续在3rd-6th和12th手术后的月。对这些题目的评价包含了常规物理考试(包括的将军和andrological考试)和精液分析。subjects’；搭挡们也经历了监视因为由月刊的怀孕关于月经会见，如果必要，尿测试。处于怀孕率没有重要差别(0.65%为IVD--一，0为IVD-B并且0.21%为NSV)在三个组之中(P>；0.05)。在12th手术后的月分别地在三个组是零，0.9%和1.7%。在结论，IVD男消毒展出长期的不利事件的低风险并且被发现作为一个男消毒方法有效，类似于NSV技术。IVD男消毒被期望是一个新奇避孕方法。

简介：Purpose:Thepurposeofthepresentstudywastoinvestigatetheage-relatedchangesintheenduranceperformanceamongmaleamateurmarathonrunners.Methods:Subjectsweretakenfromthe36StockholmMarathonsheldfrom1979through2014,andageandfinishingtimewereanalyzedforatotalof312,342malerunners.Results:Therelationwasfoundtobeasecond-orderpolynomial,t=a+bx+cx~2,whichmodels99.7%ofthevariationintheaveragerunningtimetasafunctionofagex.Themodelshowsthatthemarathonperformanceoftheaveragerunnerimprovesuptoage34.3±2.6years,thereafter,theperformancestartstodecline.Aquantificationoftheage’sinfluenceonrunningtimeshowsthatitaccountsfor4.5%ofthetotalvarianceseenintheperformancedata.Conclusion:Theseoutcomesindicatethattheeffectofageonperformanceinendurancerunningeventsisclearlymeasurable,quantifiable,andpossibletodescribe.Atthesametimethefindingsindicatethatotherfactors,suchastraining,affecttheperformancemore.Acomparisonwiththeeliteshowedpeakperformanceatthesameage,buttheratesofchangeinperformancewithage,improvementaswellasdegradation,wasfoundtobehigheramongtheelite.

简介：包括16不育系的米饭isonuclear-alloplasmic线和他们的维护者线的四个集合被使用91份SSR教材学习原子染色体和他们的相对关系的基因差异分析。169等位基因的一个总数在16根线被检测，与53.85%的多态的loci和每1.8的地点的等位基因的一个平均数字的频率，并且平均基因差异是0.228。无菌的线分享了的isonuclear-alloplasmicmale的四个集合146相同等位基因，相应于86.39%全部的等位基因；同时，在测试材料之中有23不同等位基因，是13.61%全部的等位基因。平均，78.70%相同等位基因和全部的等位基因的21.30%不同等位基因在isonuclear-alloplasmic不育系和他们的维护者线之间被检测。Therewere53.85%相同等位基因和在homozygousallonucleus不育系之中的全部的等位基因的46.15%不同等位基因。指纹为某不育系和维护者线被建立。测试的所有材料基于簇分析在0.2遗传距离被划分成三个组。HuanongA和HuayuA(包括的Huanong乐队HuayuB)的八根线在第一个组，KezhenA的四根线(包括KezhenB)在在第三个组的Zhenshan97A(包括的Zhenshan97B)的第二个组，和四根线。为theisonuclear-alloplasmic不育系，在Y(Yegong)类型和WA之间的类似系数(野未成功)打字或在CW(Raoping野米饭)和WA之间打到达87-98%。

简介：我们识别了米饭花的机关发展异种，称为的同样开的壳和男性无菌1(ohms1)，从indicarestorer线的子孙，Zhonghui8015与60Co光线照耀对待。ohms1异种展出了开的壳并且像词根并且象palea一样组织在花药和耻辱之间的变换，它导致了显示出‘tridentatelemma’的ohms1异种小穗状花小穗。ohms1异种是完全无菌却有的60%-70%肥沃的花粉。印射的基因分析和基因证明ohms1被单个后退的基因控制，并且变异的基因对在标记KY2和KY29之间的染色体3的短手臂上的42-kb间隔印射罚款。在这个区域的四个开的读物框架的顺序分析表明异种在第五intron的最后底带了单个核苷酸转变(到G的A)，它多半被通信到ohms1phynotype，在一种MIKC类型疯盒子的基因OsMADS1(LOC_Os03g11614)。进一步定序的酶消化和cDNA显示可变拼接在MADS领域或氨基酸框架为在ohms1，而是没有结构的变化的第六exon的删除负责移动出现了。另外，即时荧光灯量的PCR分析证明OsMADS1表示水平在ohms1异种显著地减少了。发展相关的基因也显著地改变了的米饭flowering因素和花的颖的表示层次。这些结果证明OsMADS1可以在米饭起一个重要作用花的机关开发，特别地在花的颖开发和小花原基区别。

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简介：AbstractObjective:This study aimed to screen for novel mutations in ACTL7A and expand the spectrum of known mutations responsible for recurrent fertilization failure.Methods:Whole-exome sequencing was performed on samples from couples who experienced recurrent assisted reproductive technology failure and visited the General Hospital of Ningxia Medical University. Western blotting and quantitative Real-time PCR were used to investigate the effects of the mutation on HEK293T cells.Results:Samples from 12 couples with total fertilization failure or poor fertilization (fertilization rate <20%) were subjected to whole-exome sequencing, and a novel homozygous protein-truncating mutation (c. 1101dupC, p. S368Qfs*5) in ACTL7A was identified in a patient with recurrent poor fertilization. The mutant resulted in a truncated protein as well as decreased protein expression level in HEK293T cells.Conclusions:Our findings expand the mutational and phenotypic spectrum of ACTL7A, thus providing a potential diagnostic marker for fertilization failure due to male factors.

简介：本文基于语料库研究的方法,运用在线语料库检索工具SketchEngine,根据英语国家语料库（BNC）中大量的事实语料,对MALE和FEMALE两个名词的词汇语法搭配行为和模式进行比较研究,从而为性别语言差异的研究提供更加有力的数据支持,使研究结果更加具有可信度和说服力。

简介：Purpose:Thisstudywastoinvestigatetheassociationbetweenulnarvariance(UV)andbiologicalandtrainingcharacteristics,handgrip,andwristpaininagroupof23Portugueseskeletallyimmaturemalegymnasts(aged11.22.5years).Methods:LeftandrightUVwasobtainedusingHafner'sprocedureandskeletalagewasdeterminedbytheTannereWhitehouse3-method.AnegativemeanvalueforUVmeasureswasobserved(2.4to3.6mm)withoutsignificantdifferenceswithincreasingage-category(p=0.09top=0.48).SignificantlowcorrelationswereobservedbetweensomeUVparametersandstature,fatpercentage,yearsoftraining,andlefthandgripstrength.Results:TengymnastsreportedwristpainwithgradualonsetandUVvalueswereverysimilarbetweenpainlessandpainfulwrists.Conclusion:ThefindingsofthisstudydonotdirectlysupportthethesisthatgymnasticstrainingandbiologicalvariablesorwristpainareassociatedwithUV.