简介：客观：调查成年钝脾的损害的非起作用的管理的指示。方法：回顾的评论在所有成年病人(年龄>15年)上被执行，钝脾的损害从1999～2003在法国承认了到Pellegrinhospital的脉管的外科的部门。我们不管年龄在所有适当病人non-operatively管理了脾的损害。结果：在4年期间，有钝脾的损害的54个连续成年病人在医院里被对待。有稳定的血液动力学的地位的27个病人的一个总数起初non-operatively被对待，哪个2个病人被辜负到non-operativetreatment。非起作用的管理的成功的百分比是92.6%。在54个病人，比55年老的7个of8病人与非起作用的管理被对待。二个案例developingpostoperatively代替膈的感染被合适的治疗愈合。在系列，没有死亡。结论：低档的非起作用的管理脾的损害能与可接受的低失败的率被完成。如果临床并且对外科医生到困难的实验室参数作决定，他们能取决于Resciniti“获得系统与脾的损伤选择成年人的一个子集的sCT(计算断层摄影术)是non-operativemanagement的试用的优秀候选人。比55年老的病人绝对没被禁止收到non-operativemanagement。

简介：Objective:Toexplorechemicalcomponentchangesofdogboneatdifferentlengtheningtimeandindifferentboneregionsofinterest,andtoevaluatethemineralizationduringIiizarovlengtheningprocess.Methods:Theashweight,theconcentrationsofcalcium,phosphorusandthecalcium/phosphorusratioweremeasuredatdifferentintervals(2,4,6,8,12weeks)sincelengtheningandthelengthenedpartwascomparedwithacontrolareaateachinterval.Results:Theashweight,theconcentrationsofcalciumandphosphorusinthelengthenedareadifferedatalldevelopmenttime.Thecalcium/phosphorus(Ca/P)ratiointhelengthenedregionremainedsignificantlylowerthanthatinthecontrolregionupto12weeksafterthelengthening.Conclusions:Theseresultssuggestthatalsootherinorganicionsplayanimportantroleinthemineralizationprocessandthattheybecomerelativelymoreimportantsince8weeksafterthelengthening.

简介：Objective:Toinvestigatetheproliferativeeffectofkeratinocytegrowthfactor(KGF-2)onhumanadultkeratinocytes.Methods:Thestandardmediumwaskeratinocytegrowthmediumwithoutbovinepituitaryextract(BPE),hydrocortisoneorepidermalgrowthfactor(EGF).Keratinocytesfroma48-year-oldsubjectwereculturedandseededondisheswithstandardmediumofEGFincelldensityof2×104/32mm2.After24hours,themediumwasreplacedbythestandardmediumwith0,4,16,125and500ng/mlKGF-2,respectively.ThestandardmediumwithEGFwasusedasthepositivecontrolandthestandardmediumwithoutEGForKGF-2wasusedasthenegativecontrols.Thegrowthofkeratinocyteswasmonitoredby3-(4,5-dimethythiazol-2-yl)-2,5dipheyltetrazoliumbromide(MTT)assayandbyphotographsondays3,5and7,respectively.Results:KGF-2inconcentrationsof4-500ng/mlshowedasignificantproliferativeeffectondays5and7ascomparedwiththatofthenegativecontrols(P<0.01).Onday3thecellswereproliferatedto1.5-2.5-fold,onday5to3-5-foldandonday7to3-12-foldinKGF-2mediumasthatofthenegativecontrols.TheoptimalresponseoccurredwhentheconcentrationofKGF-2was125ng/mlonday7.CellproliferationwasalsoconsistentlyhigherinallKGF-2concentrationsascomparedwiththatofthepositivecontrols.Conclusions:KGF-2hassignificanteffectsontheproliferationofadultkeratinocytes,whicharemoreeffectivethanthatofEGF.ThisstudysupportsKGF-2canimprovethehealingofchronicwoundsinadultsinclinic.

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简介：ObjectiveTo观察激活p21的kinase6(PAK6)在在成年rat.MethodsSprague-Dawley老鼠的针的绳索损害(SCI)以后的表示和它的可能的角色受到针的绳索损害。为了探索PAK6，表示模式和PAK6的分发的病理学、生理的意义，没被西方的污点观察，免疫组织化学和immunofluorescence.ResultsWestern污点分析证明PAK6蛋白质水平在白天2和白天4上是显著地起来调整的，然后减少了并且有到白天14为止的起来规定。免疫组织化学分析证明PAK6的表示显著地在与控制组相比的白天4上被增加。而且，染色的两倍immunofluorescence证明PAK6首先在控制组在神经原和星形细胞被表示。当时在损害以后，PAK6的表示在星形细胞显著地被增加，神经原，和星形细胞大部分被增殖。我们也检验了增殖的房间的表示原子抗原(PCNA)并且发现它的变化与PAK6的表示被相关。重要地，在在受伤针的绳索的PAK6的injury.ConclusionThe起来规定可以与glial增长被联系以后，染色的两倍immunofluorescence表明PCNA评估的房间增长在白天4上出现在许多PAK6-express-ing房间。

简介：Objective:Toinvestigatethedifferentiativecapabilityofadulthumanbonemarrowmesenchymalstemcells(BMSCs)intoSchwann-likecells.Methods:BonemarrowswereaspiratedfromhealthydonorsandmononuclearcellswereseparatedbyPercolllymphocytesseparationliquid(1.073g/ml)withcentrifugation,cellswereculturedinDMEM/F12(1:1)mediumcontaining10%fetalbovineserum(FBS),20ng/mlepidermalgrowthfactor(EGF)and20ng/mlbasicfibroblastgrowthfactor(bFGF).Cellsofpassage1wereidentifiedwithimmunocytochemistry.Conclusions:BonemarrowcontainsthestemcellswiththeabilityofdifferentiatingintoSchwann-likecells,whichmayrepresentanalternativestemcellsourcesforneuraltransplantation.