简介:Aspontaneousmutation,tentativelynamedd63,wasderivedfromthetwin-seedlingprogeniesofricecrossedbydiploidSARIIIandMinghui63.Comparedwithwild-typeplants,thed63mutantshowedmultipleabnormalphenotypes,suchasdwarfism,moretillers,smallerflagleafandreducedseed-settingrateand1000-grainweight.Inthisstudy,twoF2populationsweredevelopedbycrossingbetweend63andNipponbare,d63and93-11.Geneticanalysisindicatedthatd63wascontrolledbyasinglerecessivegene,whichwaslocatedontheshortarmofchromosome8,withinthegeneticdistanceof0.40cMfromRM22195.Hence,D63mightbeanewgeneastherearenodwarfgenesreportedontheshortarmofchromosome8.
简介:Themodernizationandinformatizationmanagementofancientandfamoustrees’managementisanimportantstandardwithwhichthemunicipalpublicresourcemanagementlevelofaregionorcitycanbemeasured.Designanddevelopmentofancientandfamoustrees’three-dimensionalmanagementinformationsystemwasrealizedbasedonvirtualplantbyusingintegratedtechniquesofvirtualplantsandGeographicInformationSystem.Thesystemarchitecturedesignwasdevelopedonthebasisoffunctionalrequirements,andthepracticalsystemwasachievedinVisualStudio2008developmenttoolsandOpenGLgraphicsstandards.Thesystemhasfunctionofancienttreesarchivesmanagement,three-dimensionalreconstructionofthetreessurroundingenvironment,individualtreeinformationinquiryinthree-dimensionalscene,etc.Applicationanddisseminationofthissystemwillgreatlypromotethemanagementandprotectionofancientandfamoustreesstandardization,informalizationandvisualization
简介:为建立高纯度bacillomycinD(杆菌霉素D)脂肽的可放大制备工艺,促进其在农用抗生素领域应用价值的开发,经分离纯化及分子结构鉴定,确定了海洋源解淀粉芽孢杆菌BacillusamyloliquefaciensTYg3-2所产活性化合物为bacillomycinD脂肽类抗生素;选择精制过的天然培养基(酵母提取物和蛋白胨),与组分明确的速效碳源、氮源(葡萄糖和硫酸铵)组成复合培养基,结合响应面优化调整培养基配方,建立了bacillomycinD发酵水平高达990mg/L的5L罐发酵工艺,其20.62mg/(L·h)的比生产速率远高于文献报道的10.23mg/(L·h);在高品质原料基础上,发酵液经菌液分离、酸化沉淀、甲醇浸提、乙醚沉淀、大孔树脂吸附及硅胶柱层析工艺,可高效制备纯度达94.72%的样品。针对所制备的高纯度bacillomycinD样品进行了抑菌活性初步研究,证实其可离体拮抗德氏霉菌、玉米弯孢霉、稻绿核菌和长柄链格孢,并通过盆栽试验证实了bacillomycinD能提升同源芽孢杆菌TYg3-2的防效,500mg/L的bacillomycinD与4×10~6CFU/mL的TYg3-2菌体混合施用,对黄瓜炭疽病的防效可达95.3%,优于单独施用bacillomycinD(78.4%)和菌体(0)的防治效果。研究初步探索了bacillomycinD在农用抗生素领域的开发价值,同时其高纯度脂肽可放大制备工艺的建立至关重要,不仅能在保全生产菌(其芽孢为生防菌剂原料)的同时从其发酵液中分离和纯化目标脂肽,以促成对农用抗生素价值的深入研究,也能够满足中国农药登记相关毒理学试验对样品的要求(纯度〉85%的克量级样品),其结果可为芽孢杆菌源脂肽类抗生素的研究与开发提供参考。
简介:采用离体抑菌试验研究观察H2O2-Ag+复方消毒剂对柑橘青霉病菌(Penicilliumitalicum)和绿霉病菌(Penicilliumdigitatum)生长的直接抑制效果,并以年橘为试材,分别活体接种P.italicum和P.digitatum,研究H_2O_2-Ag~+复方消毒剂处理对青霉病和绿霉病的抑制作用,同时分析果实在贮藏过程中的品质变化。离体抑菌试验表明:培养基中添加H_2O_2-Ag~+显著抑制了P.italicum和P.digitatum的菌丝生长(P〈0.05),并表现出明显的浓度效应,浓度为1%H_2O_2-20mg/LAg~+的复方消毒剂对两种病菌的抑制率达100%;活体接种试验表明:3%H_2O_2-60mg/LAg~+浓度的复方消毒剂可显著抑制病原菌诱导的病斑扩展,降低果实发病率,而对果实的品质及果面色差值没有明显影响。本研究表明,H_2O_2-Ag~+复方消毒剂不仅对柑橘青、绿霉菌有直接的杀灭作用,还能有效控制果实的腐烂率,可作为一种新型、安全、环保的保鲜防腐剂,应用于柑橘果实采后病害的控制。