简介:Humanextracellularsuperoxidedismutase(hEC-SOD)isasecretedtetramericproteininvolvedintheprotectionofahumanbodyfromoxygenfreeradicals.Itsthree-dimensionalstructurehasnotbeenconfirmed,hEC-SODcouldn′tbeexpressedinE.coll.Weconstructedahybridenzyme,whichcomprisestheNterminalandC-terminaldomainsfromhEC-SOD,fusedittohumanCu,Zn-SOD.ThehybridenzymeisexpressedsuccessfullyinE.coli.Further,weanalyzedtheexpressionofhEC-SODinE.colibymRNAdifferentialdisplaying.
简介:Anewbiodegrablehydrogelbasedonα,β-poly-aspartamidewassynthesizedbypolycondensationofDL-asparticacidand85%phosphoricacidinvacuityat180°C,andthenbyring-openingwith3-aminopropanoltogetherwithcross-linkingreactionwithethanediamimo.
简介:Objective:Toobservethetherapeuticeffectofelectroacupuncture(EA)onplasmaangiotensin(Ang*.Ⅱ),aldosterone(ALD)andatrialnatriureticpolypeptide(ANP)contentsinexperimentalcerebralinfarctionrabbitsforanalyzingtheunderlyingmechanismofacupunctureinamelioratingbloodsupplyofthebraintissue.Methods:Atotalof80rabbitswererandomizedintocontrol(n=8),pseudo-operation(n=24),model(n=24)andEA(n=24)groups.Cerebralinfarctionmodelwasestablishedbyinfusionofself-thrombusintothecarotidartery.EA(1mA,2Hz)wasappliedto'Baihui'(百会GV20)and'Shuigou'(水沟GV26)for30min,onceevery12hours.PlasmaAng-II,ALDandANPcontentsweredetectedwithradioimmunoassaymethod.Inthelater3groups,bloodsamplesweretakenat6h,24hand48haftercerebralischemia.Results:Comparedwithcontrolandpseudo-operationgroups,Ang-IIandALDcontentsofmodelgroupat6h,24hand48haftercerebralischemiaincreasedsignificantlywhileplasmaANPofthe3time-coursesofmodelgroupdecreasedconsiderably(P<0.01).Incomparisonwithmodelgroup,resultsshowedthatAng-IIandALDcontentsofEAgroupdecreasedsignificantlywhereasANPlevelofEAgroupincreasedstrikingly(P<0.01).Conclusion:ElectroacupuncturehastheeffectsofraisingplasmaANPlevelandloweringplasmaAng-IIandALDincerebralinfarctionrabbits.
简介:ObjectivesInvestigatedtheeardioproteetiveandmechanismsoflosartanonwholeisolatedisehemiereperfusedratheart.MethodsLangendorffperfusedsystemswasusedtoinvestigatelosartaneffectonwholeisolatedratheartsinCPK,LDH,MDA,SOD,angⅡandarrhythmia.ResuitsLosartandecreasedincidenceofarrhythmia,improvedatrialventrieularblockrecoveryinreperfusionperiod,duringisehemieperiod,CPKandLDHinI/Rgroupincreasedsignificantlycomparedwithcontrolgroup,51.33±27.02vs22.42±13.33,31.80±4.56vs22.28±15.96,respectively,butgreatlydecreasedinlosartangroupcomparedwithI/Rgroup,23.90±21.74vs51.33±27.02and11.50±13.20vs31.80±4.56,respectively.DuringreperfusionperiodCPK,LDHincreasedsignificantlyinI/Rgroupcomparedwithcontrolgroup,49.11±20.63vs12.14±5.92and28.70±4.69vs23.10±21.38,respectively,butdecreasedgreatlyinlosartangroupcomparedwithI/Rgroup,39.40±9.60vs49.11±20.63and14.50±13.75vs28.70±4.69.ThecontentofMDA,angIIinI/Rgroupmyoeytesishigherthancontrolgroup's,26.±9.25vs17.2±3.37and8.43±3.81vs4.80±0.20.HoweverthecontentofSODintwogroupshasnosignificantlychange,148.20±8.72vs145.08±6.82.thecontentofMDAinlosartangroupmyocardialtissueismuchlowerthancontrolgroup,15.92±4.05vs26.80±9.25andthecontentofangⅡinlosartangroupmyocardialtissueismuchhigherthanI/Rgroup,12.44±6.09vs8.43±3.21.ThedepartmentofcardiologyofsecondhospitalofTianjinmedicaluniversityTianjin300211However,SODhasnosignificantchangeintwogroups,143.47±7.91vs145.08±6.82.ConclusionsLosartanagainstisehemie-reperfusioninjuryofwholeisolatedrathearts,thosebeneficialeffectsaremediateprimarilybytheinhibitedofangiotensinⅡbindingwithitsreceptorandinhibitedoxygenfreeradicalscavengingpotential.
简介:Thefarmlandtoforestprojecthasasignificantpromotingroleonecologicalcivilization;thefollow-upindustrydevelopmentisthekeytoconsolidatingachievementsofthefarmlandtoforestproject.Usingthesurveydataoffarmlandfarmers,thisarticleanalyzesthestatusanddevelopmentofthefollowupindustryinGuizhouprovince.Theresultsshowthatthefollow-upindustrydevelopmentqualityislow,thefollow-upindustrydevelopmentwillingnessisweak,thefollow-upindustrydevelopmentabilityispoor,andthefollow-upindustrysustaineddevelopmentisinsufficient.Furtheranalysisindicatesthatthefollow-upindustrydevelopmentlackoffunds,technicalandsystemofpolicysupport.Policyimplicationsarediscussedfocusingonimprovingpolicysystem,thefollow-upindustrydevelopmentstrategy,thefollow-upindustrydevelopmentplanning,anddevelopmentwiththecombinationofdevelopmentstrategyofGuizhouprovince.
简介:AIM:Todeterminethealterationsinratenterocytemitochondrialrespiratoryfunctionandenzymeactivitiesfollowingtraumaticbraininjury(TBI).METHODS:Fifty-sixmaleSDratswererandomlydividedintosevengroups(8ratsineachgroup):acontrolgroup(ratswithshamoperation)andtraumaticbraininjurygroupsat6,12,24h,days2,3,and7afteroperation.TBImodelswereinducedbyFeendy’sfree-fallingmethod.Mitochondrialrespiratoryfunction(respiratorycontrolratioandADP/Oratio)wasmeasuredwithaClarkoxygenelectrode.TheactivitiesofrespiratorychaincomplexⅠ-Ⅳandrelatedenzymesweredeterminedbyspectrophotometry.RESULTS:Comparedwiththecontrolgroup,themitochondrialrespiratorycontrolratio(RCR)declinedat6handremainedatalowleveluntilday7afterTBI(control,5.42±0.46;6h,5.20±0.18;12h,4.55±0.35;24h,3.75±0.22;2d,4.12±0.53;3d,3.45±0.41;7d,5.23±0.24,P<0.01).Thevalueofphosphate-to-oxygen(P/O)significantlydecreasedat12,24h,day2andday3,respectively(12h,3.30±0.10;24h,2.61±0.21;2d,2.95±0.18;3d,2.76±0.09,P<0.01)comparedwiththecontrolgroup(3.46±0.12).Twotroughsofmitochondrialrespiratoryfunctionwereseenat24handday3afterTBI.TheactivitiesofmitochondrialcomplexⅠ(6h:110±10,12h:115±12,24h:85±9,day2:80±15,day3:65±16,P<0.01)andcomplexⅡ(6h:105±8,12h:110±92,24h:80±10,day2:76±8,day3:68±12,P<0.01)wereincreasedat6hand12hfollowingTBI,andthensignificantlydecreasedat24h,day2andday3,respectively.However,therewerenodifferencesincomplexⅠandⅡactivitiesbetweenthecontrolandTBIgroups.Furthermore,pyruvatedehydrogenase(PDH)activitywassignificantlydecreasedat6handcontinuedupto7dafterTBIcomparedwiththecontrolgroup(6h:90±8,12h:85±10,24h:65±12,day2:60±9,day3:55±6,day7:88±11,P<0.01).Thechangesinα-ketoglutaricdehydrogenase(KGDH)activityweresimilartoPDH,exceptthatthedecreaseinKGDHactivitybeganat12hafterTBI(12h:90±12,24h:80±9,day2:76±15,day3:68±7,
简介:Zygosaccharomycesrouxiiisasalt-tolerantyeastspeciescapableofremovingcadmium(Cd)pollutantfromaqueoussolution.Presently,thephysiologicalcharacteristicsofZ.rouxiiunderthestressofsodiumchloride(NaCl)andCdarepoorlyunderstood.ThisstudyinvestigatedtheeffectsofNaClandCdonthegrowth,oxidativestressandantioxidantenzymeactivitiesofZ.rouxiiafterstresstreatmentfor24h.ResultsshowedthatNaClorCdalonenegativelyaffectedthegrowthofZ.rouxii,butthegrowth-inhibitingeffectofCdonZ.rouxiiwasreducedinthepresenceofNaCl.FlowcytometryassayshowedthatunderCdstress,NaClsignificantlyreducedtheproductionofreactiveoxygenspecies(ROS)andcelldeathofZ.rouxiicomparedwiththoseintheabsenceofNaCl.Theactivitiesofsuperoxidedismutase(SOD),catalase(CAT),andperoxidase(POD)ofZ.rouxiiweresignificantlyenhancedby2%–6%NaCl,whichlikelycontributedtothehighsalttoleranceofZ.rouxii.ThePODactivitywasinhibitedby20mgL-1CdwhiletheSODandCATactivitieswereenhancedby8mgL-1Cdandinhibitedby20mgL-1or50mgL-1Cd.Theinhibitoryeffectofhigh-levelCdontheantioxidantenzymeactivitiesofZ.rouxiiwascounteractedbythecombineduseofNaCl,especiallyat6%.ThisprobablyaccountedforthedecreaseinCd-inducedROSproductionandcelldeathofZ.rouxiiafterincubationwithNaClandCd.OurworkprovidedphysiologicalcluesastotheuseofZ.rouxiiasabiosorbentforCdremovalfromseawaterandliquidhighlysaltyfood.
简介:在土壤和蔬菜的锑(Sb)的高集中能引起潜在的健康风险。然而,庄稼的生长和反应上的Sb的效果不是众所周知的,迄今为止,仍然为在中国的AlliticUdicFerrisols没有Sb限制标准。在这研究,一个温室实验被执行在生物资源,生理的表演,和宏上调查锑(Sb)的效果--并且绿中国卷心菜(BrassicachinensisL.)的微量元素元素集中,以及酶活动在从湖南省的AlliticUdicFerrisols,中国。锑以0的率(控制)被供应,2,5,10,20,和50mgkg−1并且因此在对待的土壤样品1.0mgkg−1,与背景珍视Sb集中是1,3,6,1121,并且51mgkg−1,分别地。结果显著地显示出卷心菜的叶生物资源和维生素酸内容(P<0.05)分别地,在30.6%和48.3%减少了并且显著地也玷污urease和脱氢酶活动(P<0.05)分别地,在33.6%和32.5%减少了什么时候玷污Sb集中,作为与控制相比是21mgkg−1。象由卷心菜的Mg,Cu,和Zn那样的必要滋养的元素的举起显然被影响,当时当土壤Sb集中超过了21mgkg−1时,可溶的糖内容稍微改变了的叶。把生理的回答和土壤酶活动基于卷心菜,为在AlliticUdicFerrisols的Sb的21mgkg−1的可允许的集中应该被推荐。
简介:Afterprimaryanalysesontheserumglycocon-jugatesoflungcancerandnormalindividulusingtheenzyme-linkedlectinassay(ELLA)with12kindsoflectins,PHAandLCAwereselectedtofurtherstudyintheseraof8kindsofcancers,4kindsofnon-malignantdiseaseand1kindofpostoperativecancer.Itwasfoundthatthetestvaluesof7kindsofcancerswithPHAorLCAweresignificantlyhigherthanthatofthenormal(P<0.01);thevaluesof4kindsofnon-malignantdiseaseswithPHAwerenothigher(P>0.05);thevaluesofthepostoperativecancerwithPHAwereobviouslylowerthanthatofthepreoperative(P<0.05).TheresultsshowedthattheserumglycoconjugateswhichcanbindtoPHAseemedrelatedtothecancerousexistenceinhumanbodies.Thesignificanceofthefindingswasdiscussed.
简介:淀粉,直链淀粉,蔗糖内容和在在开花以后的shading处理下面的淀粉合成酶的活动的动态变化作为材料用二米饭变化IR72(indica)和Nipponbare(装饰用的梨树)被学习。当仅仅有点改变的自动数据处理葡萄糖pyrophosphorylase(ADPGPPase)活动,可溶性淀粉synthase活动和小粒绑了淀粉synthaseactivity时,在shading处理,thest拱门,直链淀粉和蔗糖下面,内容减少了减少,可溶性淀粉分叉酶(SSBE,Q酶)活动和小粒界限淀粉分叉酶(GBSBE,Q酶)增加的活动,和淀粉初上舞台的人ranching酶(DBE,R酶)活动与变化变化了。关联分析证明淀粉内容的变化断然并且显著地在弱光与蔗糖内容的变化被相关。ADPGPPase活动和SSBE活动断然并且显著地与淀粉累积率被相关。淀粉synthase活动的衰落与淀粉内容的减少有关,淀粉分叉酶的活动的增加在弱光下面在直链淀粉的比率的减少起了一个重要作用到全部的淀粉,这被暗示。
简介:Effectsofshadingatthefloweringandgrainfillingstagesonosmoticregulationsubstancecontentandprotectiveenzymeactivitiesintheleavesofdifferentricegenotypes(e.g.,IIyou498,Gangyou188,Dexiang4103,Gangyou527andChuanxiang9838)wereinvestigated.TheresultsshowedthatthemalondialdehydecontentofGangyou188significantlyincreasedundershading,whereasthoseofIIyou498,Dexiang4103,Gangyou527andChuanxiang9838showednosignificantdifferencescomparedtothecontrol.ThesolublesugarcontentsignificantlydecreasedinIIyou498,Gangyou188,Dexiang4103andChuanxiang9838,whereasitdidnotsignificantlyincreaseinGangyou527undertheweaklightconditions(shading).Moreover,thesolubleproteincontentintheleavesofdifferentgenotypestendedtodecreaseundershading.Further,theprotectiveenzymeactivitiesintheleavesvariedindifferentricegenotypesundertheshadingtreatment.Basedontheosmoticregulationsubstancecontentandtheprotectiveenzymeactivitiesintheleavesofdifferentricegenotypes,itwasconcludedthatGangyou188andGangyou527hadstrongabilitiestoadapttothelowlightconditions.Inaddition,themechanismofdamagetoriceleavesindifferentgenotypesundershadingwasdiscussed.
简介:BACKGROUND:RecentstudieshavesuggestedthatmitochondrialATP-sensitiveK+channelopenerscouldreducemyocardiuminfarctsize,andprotectthefunctionofthemitochondria.OBJECTIVE:ToinvestigatethechangesofcerebralinfarctionvolumeandtheactivityofmarkerenzymesinbrainmitochondriaofratsgiventheATP-sensitiveK+channelopener,nicorandil,beforefocalcerebralischemia/reperfusion(I/R).DESIGN,TIMEANDSETTING:Randomized,controlledanimalexperiment,completedattheBrainScientificResearchCenteroftheAffiliatedHospitalofQingdaoUniversityfromJulytoNovember2007.MATERIALS:SixtyhealthymaleWistarratsweighing280–300g.Nicorandil,5-hydroxydecanoate(5-HD)andcytochromeCwerepurchasedfromSigmaintheUSA.Standardmalondialdehyde(MDA)andproteinwerepurchasedfromNanjingJianchengBiotechnologyInstitute.METHODS:Sixtyratswererandomlydividedintoashamoperationgroup,amiddlecerebralarteryocclusion(MCAO)group,anicorandilgroupandanicorandil+5-HDgroup.MCAOfor2hourswasperformedintheMCAOgroup,nicorandilgroupandnicorandil+5-HDgroup.Atotalof5mLsalineweregiventotheMCAOgroupbeforeMCAO.ThenicorandilgroupwasinjectedwiththeATP-sensitiveK+channelopenernicorandil10mg/kgintraperitoneally30minutesbeforeMCAO.Thenicorandil+5-HDgroupwasinjectedwith5-HD10mg/kgintravenously15minutesbeforethesametreatmentasthenicorandilgroup.MAINOUTCOMEMEASURES:Infarctvolumebytotalbrainslicecalculation,activitiesofsuccinatedehydrogenase(SDH)andcytochromeoxidase(CO),andcontentofMDAwereobservedat22hoursofreperfusionafter2hoursMCAO.RESULTS:Sixtyratswereincludedinthefinalanalysis,withoutanyloss.(1)Infarctvolume:comparedwiththeMCAOgroupandnicorandil+5-HDgroup,thepercentageofinfarctvolumewassignificantlydecreasedinthenicorandilgroup(P<0.01).(2)ThecontentofMDA,expressionofSDHandCOinbrain:theexpressionsofSDHandCOintheshamop
简介:ObjectivesToinvestigateeffectofAngll,captoprilonsingleguineamyocytesonL-typecalciumcurrentandsodiumcurrent.MethodsMembranepatchclampwholecellrecordingtechniquewasusedtoinvestigateeffectofangll,captoprilonL-Camaximumcurrentdensityandsodiummaximumcurrentdensity.ResutlsAngllincreasedthemaximumcurrentdensitycomparedwithcontrolafterperfused5min,357.7±219.7Vs279.5±240.5PA/PF,increaserateis27.9%,theshapeofcurrent-voltagerelationshipcurvewasunchanged,peakedat+10mv,indicatedthatangllincreasedL-Cacurrentdensityinvoltage-dependent.Afterperfusedwithcaptopril,captopril+angll3,5min,L-Cacurrentwasrecorded,resultssuggestL-Camaximumcurrentdensitydecreasedsignificantlycomparedwithcontrol,incaptoprilgroup,128.4±92.6Vs286.2±89.7,66.7±68.3Vs286.2±89.7,respectively,rateofinhibitionis55.1%,76.6%,respectively.L-Cacurrentfurtherdecreasedincaptoprilpe
简介:在flowering舞台被使用二装饰用的梨树米饭变化Koshihikari和Sasanishiki在天赋和高温下面学习以后,蔗糖,fructose,蔗糖synthase的葡萄糖内容和差别迟钝,vacuolar转化酶,和细胞壁的动态变化在米饭谷物绑了转化酶。在米饭谷物,蔗糖synthase活动比转化酶的高,它显著地与淀粉累积率被相关,显示蔗糖synthase起了在蔗糖降级和淀粉合成的一个重要作用。在高温下面,没有fructose和葡萄糖的任何增加的谷物蔗糖内容的重要增加满足,建议高温处理提高了蔗糖累积,当减少时在米饭谷物的蔗糖降级。与控制植物相比,在蔗糖synthase,vacuolar转化酶,和有高温的细胞壁界限转化酶的活动的减少对待显示的植物蔗糖降级的减速与在蔗糖synthase和转化酶的活动的减少有关。
简介:AbstractBackground:Macrophages are involved in the pathogenesis of idiopathic pulmonary fibrosis, partially by activating lung fibroblasts. However, how macrophages communicate with lung fibroblasts is largely unexplored. Exosomes can mediate intercellular communication, whereas its role in lung fibrogenesis is unclear. Here we aim to investigate whether exosomes can mediate the crosstalk between macrophages and lung fibroblasts and subsequently induce fibrosis.Methods:In vivo, bleomycin (BLM)-induced lung fibrosis model was established and macrophages infiltration was examined. The effects of GW4869, an exosomes inhibitor, on lung fibrosis were assessed. Moreover, macrophage exosomes were injected into mice to observe its pro-fibrotic effects. In vitro, exosomes derived from angiotensin II (Ang II)-stimulated macrophages were collected. Then, lung fibroblasts were treated with the exosomes. Twenty-four hours later, protein levels of α-collagen I, angiotensin II type 1 receptor (AT1R), transforming growth factor-β (TGF-β), and phospho-Smad2/3 (p-Smad2/3) in lung fibroblasts were examined. The Student’s t test or analysis of variance were used for statistical analysis.Results:In vivo, BLM-treated mice showed enhanced infiltration of macrophages, increased fibrotic alterations, and higher levels of Ang II and AT1R. GW4869 attenuated BLM-induced pulmonary fibrosis. Mice with exosomes injection showed fibrotic features with higher levels of Ang II and AT1R, which was reversed by irbesartan. In vitro, we found that macrophages secreted a great number of exosomes. The exosomes were taken by fibroblasts and resulted in higher levels of AT1R (0.22 ± 0.02 vs. 0.07 ± 0.02, t = 8.66, P = 0.001), TGF-β (0.54 ± 0.05 vs. 0.09 ± 0.06, t= 10.00, P < 0.001), p-Smad2/3 (0.58 ± 0.06 vs. 0.07 ± 0.03, t= 12.86, P < 0.001) and α-collagen I (0.27 ± 0.02 vs. 0.16 ± 0.01, t = 7.01, P = 0.002), and increased Ang II secretion (62.27 ± 7.32 vs. 9.56 ± 1.68, t= 12.16, P < 0.001). Interestingly, Ang II increased the number of macrophage exosomes, and the protein levels of Alix (1.45 ± 0.15 vs. 1.00 ± 0.10, t = 4.32, P = 0.012), AT1R (4.05 ± 0.64 vs. 1.00 ± 0.09, t = 8.17, P = 0.001), and glyceraldehyde-3-phosphate dehydrogenase (2.13 ± 0.36 vs. 1.00 ± 0.10, t = 5.28, P = 0.006) were increased in exosomes secreted by the same number of macrophages, indicating a positive loop between Ang II and exosomes production.Conclusions:Exosomes mediate intercellular communication between macrophages and fibroblasts plays an important role in BLM-induced pulmonary fibrosis.