简介：Toinvestigatetheetiologyofgenitalulcerdisease(GUD)amongpatientsattendingsexuallytransmitteddisease(STD)clinicsinGuangzhou,China.BetweenSeptember8,1998,andAugust9,assessed.ClinicaletiologyofGUDwasdependentonincludingthefollowing:darkfieldmicroscopyandserologyforTreponemapallidum(TP),swabsofgenitalulcerforsimultaneousdetectionofHSV,TP,Haemophiusducreyi(HD),Humanpapillomavirus(HPV),andserologyforHIVinfection.Twohundredthirtymenandthirty-sevenwomenwithamedianageof33.4(range16-74years)wereanalyzed.TheetiologyofGUDwassyphilis(26.59%)(71/267),genitalherpes(17.60%)(47/267),condylomataacuminata(4.87%)(13/267),candidiasis(3.37%)(10/267),andmultipeinfection(6.74%)(18/267).TheseroprevalenceofHIVwas0.07%(2/267).Noetiologywasidentifiedin50.56%(135/267).TheetiologyofGUDamongSTDpatientsinourareawasmultifactorialwithapredominanceofsyphilisandgenitalherpes.BasedonthislimiteddataobtainedatSTDclinics,HIVinfectionwasnotcommon.

简介：Objective:Toinvestigatethedepressionstatusofpatientswithsexuallytransmitteddiseases(STDs).Methods:Thedepressionstatusoffifty-onehospitalizedSTDpatientswasevaluatedinarandomizedcontrolstudyusingZung'sQuantitativeTable.18healthycontrolpatientswithsimilardemographicbackgroundswererandomlychosenascontrols.Patientswithscoresaboveorequalto40wereconsideredtobesufferingfromdepression.Results:Theprevalencerateofdepressioninthepatientgroupwasobviouslyhigherthanthatofinthecontrol(X2=16.456,P<0.01).Prevalenceofdepressionwasfoundtobesignificantlyrelatedtooccupation(P<0.05).Thoughtheprevalencewasnotfoundtodiffersignificantlybetweenthosewithatreatmentcourselessthan2monthsandthosewithonelongerorequalto2months(X2=0.041,P>0.05),themeandepressionscoresoftheformergroupweresignificantlyhigherthanthoseofthelatter(P<0.01).Nosignificantdifferenceswerefoundbetweennewversusrelapsingdisease,marriedversusnon-married,maleversusfemale,ordifferingeducationalbackgrounds.

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简介：Objective:ToanalyzeHIVreplicationandimmunefunctionchangesamongChinesepatientswithHIV/ARC/AIDSandtheirassociationwithdiseaseprogression.Methods:Tcellsubsets,plasmacytokineconcentrationsandviralloadsfrom42HIV+individuals,and13ARC/AIDSpatients,andI0controlswereanalyzedbyflowcytometry(FCM),quantitativeELISAandreversetranscription-polymerasechainreaction(RT-PCR),respectively.Results:CD4cellcountsandplasmaIL-2inHIV/AIDSpatientsweresignificantlylessthaninnormalcontrolsubjects(P<0.001).TheplasmaconcentrationsslL-2R,TNF-a,andNeopterinincreasedsignificantlywithdecreasingCD4cellcounts.PlasmaIL-2amongAIDS/ARCpatientswasalsolessthaninHIV+patients(P<0.01).CD4cellcounts,theratioofCD4toCD8andplasmaIL-2levelsdecreasedsignificantlywithinfectionduration.CD4cellcountsdeclinedanaverageof43/miperyear.Incontrast,theconcentrationofplasmaslL-2R,sTNFR-I,andNeopterinincreasedanaverageof9.03pg/ml,8.69pg/ml,2.11ng/mlperyear,respectively.Furthermore,asignificantreverselinearcorrelationwasobservedbetweenCD4cellcount,CD4/CD8ratio,andCD3,CD4andCD8countswithplasmalevelsofsTNFR-I,Neopterin,andHIVRNAload.ApositivelinearcorrelationwasnotedbetweenplasmasIL-2RlevelsandchangesofplasmaIL-6level(P<0.001),IL-10(P<0.001),TNF-a(P<0.001),sTNFR-I(P<0.005),andNeopterin(P<0.002)andbetweenIL-6andTNF-a(P<0.001),NeopterinandIL-10(P<0.05),sTNFR-I(P<0.001),plasmaviralloadandsTNFR-I(P<0.001),andNeopterin(P<0.002).Conclusion:ThesefindingssuggestacloserelationshipbetweenIL-6andTNF-almmuneactivation,HIVreplicationanddiseaseprogressioninprimaryHIVinfectionsandAIDSpatients.DecliningCD4cellsandplasmaIL-2,andincreasingviralload,sIL-2R,TNF-a,sTNFR-I,andNeopterinmightbeconsideredasimportantpredictorsoftheprogressionofHIVinfectiontoAIDS.

简介：Objectives:TodevelopamethodofsimultaneousPCRdetectionofHaemophilusducreyi,Treponemapallidum,andHerpesSimplexVirusTypes1and2fromgenitalulcersamongpatientsattendingSTDclinicsinGuangzhou,China;andevaluatetheclinicalapplicationofmultiplexPCR(M-PCR)assayfordiagnosingtheetiologyofgenitalulcerdiseases(GUD).Methods:244patientswithagenitalulcerwereevaluated.ClinicaletiologyofGUDwasbasedonphysicalappearanceandmicrobiologicevaluationsthatincludeddarkfieldmicroscopyexamination(D-F)andserologytestforsyphilis(STS).SwabsofeachgenitalulcerweretestedforHSVantigenbyenzymeimmunoassay(EIA)andprocessedinanM-PCRassayforsimultaneousdetectionofT.pallidum,HSVandH.ducreyi.Results:ThestandardstrainsofT.pallidum,HSVandH.ducreyiwereamplifiedbyM-PCR,producingamplifiedproductsof260bp,432bp,170bp,respectively.ThesensitivityofM-PCRis102pgDNA.M-PCRassayforT.pallidum,HSVandH.ducreyishowedgoodagreementwhencomparedwithD-FdetectionforT.pallidum,STS,H.ducreyicultureandEIAforHSVantigen(Kappascoresare0.774,0.704,0.793,0.756,respectively).Conclusions:TheM-PCRisaconvenient,accurateandreliableassayforthedetectionofT.pallidum,HSVandH.ducreyifromgenitalulcers,andcanbeusedasamethodofdiagnosingtheetiologyofGUD.