简介:目的:探讨胎牛血清对小鼠角膜上皮细胞生长和分化的影响。方法:分别在无血清低钙培养基(KSFM)和含100mL/L胎牛血清的KSFM中培养小鼠角膜上皮细胞。比较两种培养基中角膜上皮细胞的群体倍增(PD)。通过RT-PCR和Westernblotting方法检测p63、角蛋白19以及involucrin的表达。结果:在KSFM中,小鼠角膜上皮细胞可稳定传代超过25代,但在含胎牛血清的培养基中,细胞仅能存活3代。在KSFM中,培养细胞呈典型铺路石样外观,RT-PCR和Westernblotting结果显示细胞表达p63、角蛋白19以及involucrin。当培养基中加入胎牛血清后,细胞形态明显增大,呈扁平状;involucrin表达显著增强。结论:胎牛血清可抑制小鼠角膜上皮细胞增生、诱导其分化。
简介:Thekeratoprosthesis(KPro;artificialcornea)isaspecialrefractivedevicetoreplacehumancorneabyusingheterogeneousformingmaterialsfortheimplantationintothedamagedeyesinordertoobtainacertainvision.Themainproblemsofartificialcorneaarethebiocompatibilityandstabilityofthetissueparticularlyinpenetratingkeratoplasty.Thecurrentstudiesoftissue-engineeredscaffoldmaterialsthroughcomprisingcompositesofnaturalandsyntheticbiopolymerstogetherhavedevelopedanewwaytoartificialcornea.Althoughawideagreementthatthelong-termstabilityofthesedeviceswouldbegreatlyimprovedbythepresenceofcorneacells,modificationofkeratoprosthesistosupportcorneacellsremainselusive.Mostofthestudiesoncornealsubstratematerialsandsurfacemodificationofcompositeshavetriedtoimprovethegrowthandbiocompatibilityofcorneacellswhichcannotonlyreducethestimulusofheterogeneousmaterials,butalsomoreimportantlycontinuousandstablecorneacellscanpreventthedestructionofcollagenase.Thenecrosisofstromaandspontaneousextrusionofthedevice,allowformaintenanceofaprecornealtearlayer,andplaytheroleofensuringagoodopticalsurfaceandresistingbacterialinfection.Asaresult,improvementincornealcellshasbeenthemainaimofseveralrecentinvestigations;someefforthasfocusedonbiomaterialforitswellbiologicalpropertiessuchaspromotingthegrowthofcorneacells.Thepurposeofthisreviewistosummarythegrowthstatusofthecornealcellsaftertheimplantationofseveralartificialcorneas.
简介:AIM:Toinvestigatethediffusioncharacteristicsofwaterofopticnerveandopticradiationinhealthyadultsanditsrelatedfactorsbydiffusiontensorimaging(DTI)at3T.METHODS:Atotalof107healthyvolunteersperformedheadconventionalMRIandbilateralopticnerveandopticradiationDTI.TheprimarydataofDTIwasprocessedbypost-processingsoftwareofDTIstudio2.3,obtainingfractionalanisotropyvalue,meandiffusivityvalue,principalenginevalue,orthogonalenginevaluebymeasuring,andanalyzedbytheSPSS13.0statisticalsoftware.RESULTS:Thebilateralopticnerveandopticradiationfiberspresentedgreencolorindirectionalencodedcolor(DEC)mapsandpresentedhighsignalinfractionalanisotropy(FA)maps.TheFAvalueoftheleftopticnervewas0.598±0.069andtherightwas0.593±0.065;themeandiffusivity(MD)valueoftheleftopticnervewas(1.324±0.349)×10-3mm2/sandtherightwas(1.312±0.350)x10-3mm2/s;theprincipalenginevalue(λ?)oftheleftopticnervewas(2.297±0.522)×10-4mm2/sandtherightwas(2.277±0.526)×10-3mm2/s;theorthogonalenginevalue(λ⊥)oftheleftopticnervewas(0.838±0.285)×10-3mm2/sandtherightwas(0.830±0.280)×10-3mm2/s;theFAvalueoftheleftopticradiationwas0.636±0.057andtherightwas0.628±0.056;themeandiffusivity(MD)valueoftheleftopticradiationwas(0.907±0.103)×10-3mm2/sandtherightwas(0.889±0.125)×10-3mm2/s;theprincipaleigenvalue(λⅡ)oftheleftopticradiationwas(1.655±0.210)×10-3mm2/sandtherightwas(1.614±0.171)×10-3mn2/s;theorthogonalenginvalue(λ⊥)oftheleftopticradiationwas(0.531±0.103)×10-3mm2/sandtherightwas(0.524±0.152)×10-3m
简介:AIM:Toadaptthelowvision-relatedqualityoflife(LVQOL)instrumentintoTurkishlanguageandtoassessitsvalidityandreliability.METHODS:Thestudywasconductedin387patientsattendingtheCentreofLowVisionRehabilitation,FacultyofMedicine,AnkaraUniversity.Forstatisticalanalyses,theSpearman’scorrelationcoefficient,Cronbach’salphacoefficientandConfirmatoryFactorAnalysis(CFA)wereused.RESULTS:AccordingtoresultsofCFA,theiteminthe'Adjustment'subscalebecauseofhavingthefactorloadingbelow0.40,wasexcludedfromthequestionnaire.ThereliabilityofthequestionnairewasassessedaccordingtoCronbach’salphacoefficients.Thereliabilityofthe'DistanceVision,Mobility,andLighting'subscalewas?琢=0.863;ofthe'Adjustment'subscalewas?琢=0.694;'ReadingandFineWork'was?琢=0.791,and'ActivitiesofDailyLiving'was?琢=0.770.Sotheseresultsindicatethatthequestionnaireisreliabletomeasurethevisionrelatedqualityoflifeoflow-visionpatients.Thecorrelationsbetweenthesubscaleswerealsoanalyzed,andthecorrelationbetween'Adjustment'and'ReadingandFineWork'wasfoundtobethelowest(rs=0.336,P<0.001),whereasthestrongestcorrelationwasfoundbetweenthe'ReadingandFineWork',and'ActivitiesofDailyLiving'.Additionally,the'Adjustment'dimensionshowedthestrongestcorrelationwithonly'DistanceVision,Mobility,andLighting'dimension.CONCLUSION:Afterremovingthelastitemintheseconddimension,theTurkishadaptationofalldimensionsoftheLVQOLhasbeenshowntobereliable,validandsuitableforuseinpatientswithlowvisioninTurkey.
简介:手法小切口白内障术(manualsmallincisioncataractsurgepy,MSICS)特别是在发展中国家已广泛应用于临床手术。本文旨在比较传统白内障囊外摘除术(extracapsularcataractextraction,ECCE)、MSICS以及超声乳化白内障吸除术治疗效果以及安全性(术中并发症以及术后并发症)。通过使用PubMed搜索引擎,我们收集了传统ECCE、MSICS以及超声乳化白内障吸除术的相关文献,以评价其安全性和治疗效果。同时,也参考了国内出版的相关文献。结果发现,与超声乳化白内障吸除术相比,传统ECCE以及MSICS也取得了很好的治疗效果,并且具有并发症少等优点。MSICS不需要依赖昂贵的仪器,并且具有手术快速、费用低廉以及技术门槛低等特点,特别适用于硬核白内障的治疗。虽然在白内障手术治疗过程中MSICS需要一定的技术和耐心,但它仍然是一种安全、有效、经济治疗手段,特别是在发展中国家可以替代超声乳化进行白内障的治疗。
简介:AIM:Toinvestigatethespecialtyoftranscranialsurgerythroughpterionalapproachforremovalofcranio-orbitaltumors,introducetheophthalmologicalexperiencesofenteringtheorbittoreducetheincidencerateofassociatedcomplicationsofthisoperation.·METHODS:Weperformedaretrospectiveanalysisofaseriesof37casesinvolvingpatientswhounderwenttranscranialsurgerythroughpterionalapproachfortreatmentofcranio-orbitaltumorsinourdepartmentinthepast8years.Pterionapproachcraniotomywasperformedtoallpatients.Afterremovingtumorsintheskullbytheneurosurgeon,ophthalmologistremovedtumorsinorbit.Wetookmeasuresbelowtodecreasecomplications,includinggroundingopticcanalthroughanabrasivedrillingwhennecessary,hangingvariousextraocularmusclestobeexposedforprotection,refrigeratingbyrefrigerationheadstoremovetumors,atlastsewinguporbitseptumaftersurgery.·RESULTS:Tumorswereremovedcompletelyin32cases,andincompletein5casesduetoextensiveinvasionintothecavernoussinusorsphenoidsinus.Ofallthecases,benigntumorsweredemonstratedin28cases(75.6%,28/37)andmalignantin9(24.3%,9/37).Themostcommonlesiontypewasmeningiomain11cases(29.7%,11/37).Extraocularmuscles(EOM)impairment,occurringin21cases(56.7%,21/37),wasthemostfrequentpostoperativecomplication.Themostseriousconsequencewasvisionlossoccurredin4cases(10.8%,4/37).Othercomplications,suchas11casesoftransientblepharoptosis29.7%(11/37),5casesofmydriasisin13.5%(5/7);2casesofcerebrospinalrhinorrheain5.4%(2/37).·CONCLUSION:Cranio-orbitaltumorscanberemovedcompletelyusingtranscranialapproach,andthepterionalapproachoffersexcellentexposure.Cooperationofinterdisciplinaryteamofneurosurgeonsandophthalmologistsconducestofulluseofrespectiveprofessionaladvantages.Theexperienceofophthalmicoperationtechnologycandecreaseoccurrenceofocularcomplicationsaftersurger
简介:目的:应用Pentacam眼前节测量分析系统对高度近视眼行飞秒激光LASIK和小切口基质透镜取出术后的角膜后表面的变化进行观察比较。方法:选取2015-05/12于我院行激光角膜屈光手术的高度近视患者(-6.00D≤等效球镜度数≤-10.00D)67例132眼,根据患者接受手术方式的不同分成飞秒激光制瓣联合准分子激光原位角膜磨镶术(简称FS-LASIK组)66眼和行小切口基质透镜取出术(简称SMILE组)66眼,术中SMILE组角膜帽厚度和FS-LASIK组术中角膜瓣厚度都设置为110μm。分别于术前、术后3、6mo利用Pentacam测量患者角膜后表面高度,观察对比其术前术后的角膜后表面高度的变化量。结果:术后6mo,FS-LASIK组角膜后表面高度为6.47±1.65mm,显著高于术前5.20±1.32mm。SMILE组角膜后表面高度为6.40±1.33mm,显著高于术前5.18±1.25mm,差异均具有统计学意义(P〈0.05);术后6mo,FS-LASIK组角膜表面高度前凸量(1.29±1.28mm)与SMILE组(1.22±0.89mm)相当,差异无统计学意义(P〉0.05)。结论:FS-LASIK和SMILE矫正高度近视术后均出现一定程度的角膜后表面前突,后表面高度值增加;FS-LASIK相较于SMILE,术后早期角膜后表面变化量略大,但无明显的临床差别,SMILE术后角膜后表面形态稳定性相对略好。
简介:AIM:Toinvestigatethelevelsofserumsolubleintercellularadhesionmolecules-1(sICAM-1)andneutrophilicexpressionofCD18inpatientswithvariousstagesofdiabeticretinopathyandtodeterminetheirdifferentexpressionpatterninthedevelopmentofdiabeticretinopathy(DR).·METHODS:LevelsofserumsICAM-1andCD18onthesurfaceofneutrophileweremeasuredin41DRpatients,theywereclassifiedinthreesubgroupsaccordingtothestageofretinopathyasdeterminedbyfund’sophthalmoscopy;10controlsubjectswerealsostudied.sICAM-1weremeasuredbyenzyme-linkedimmunosorbentassayandCD18byflowcytometry.·RESULTS:TheneutrophilicCD18expressionandserumsICAM-1levelwereallsignificantlyelevatedinalldiabeticsubgroupscomparedtocontrolsubjects(P<0.01).ThedifferencesofCD18andsICAM-1amongthediabeticsubgroupsweresignificantinCD18butnotinsICAM-1.TheprogressionofretinopathywasassociatedwithanincreasebothinCD18andinsICAM-1levelsbysimplecorrelationanalysis(β=0.74,P<0.001;β=0.38,P<0.01,respectively).ButstepwisemultipleregressionanalysisrevealedthatonlyCD18wasindependentdeterminantofretinopathy(β=1.04,P<0.01).·CONCLUSION:OurresultsconfirmthecontributionofendothelialandneutrophilicactivationinthedevelopmentofDRasindicatedbyincreasedlevelsofCD18andsICAM-1.However,adirectimplicationofCD18andICAM-1intheprogressionofDRcanbesupportedonlyintheCD18butnotICAM-1.CD18andICAM-1mayplaydifferentroleinthedevelopmentofdiabeticretinopathy.
简介:目的:探讨雷珠单抗(ranibizumab)治疗特发性脉络膜新生血管(idiopathicchoroidalneovascularization,ICNV)的有效性,分析在光学相干断层扫描(opticalcoherencetomography,OCT)下不同形态表现的特发性脉络膜新生血管(Ⅰ型和Ⅱ型)的疗效差异,进一步为雷珠单抗在治疗脉络膜新生血管的有效性提供±据,指导临床治疗。方法:对我院2013-10/2014-06的31例(Ⅰ型9例,Ⅱ型22例)诊断为"ICNV冶并接受玻璃体腔注射雷珠单抗的患者资料进行回顾性分析,比较分析最佳矫正视力(best-correctedvisualacuity,BCVA)和OCT测量病灶处视网膜最大厚度的变化趋势有无不同。结果:患者31例(其中Ⅰ型9例,Ⅱ型22例)经统计学分析,在术前与术后1,3moBCVA和病灶视网膜最大厚度的比较具有统计学意义,不同ICNV类型患者治疗前后的最佳矫正视力及病灶处视网膜最大厚度的变化趋势的差异无统计学意义,说明雷珠单抗玻璃体腔注射对于治疗特发性脉络膜新生血管疗效肯定,对于Ⅰ型和Ⅱ型ICNV的临床治疗效果不具显著性差异。结论:玻璃体腔注射雷珠单抗治疗特发性脉络膜新生血管疗效肯定,对于Ⅰ型和Ⅱ型ICNV本组研究中尚未发现存在疗效差异。其安全性和远期并发症需进一步研究证实。
简介:AIM:Todeterminewhethersinglenucleotidepolymorphism(SNP)rs641153isassociatedwiththeriskofage-relatedmaculardegeneration(AMD),weperformedasystematicmeta-analysisof15eligiblestudies.SNPinthecomplementfactorB(CFB)geneisconsideredtohavesignificantassociationwithAMDsusceptibility,butthereisgreatdiscrepancyintheseresults.METHODS:TheeligiblestudieswereidentifiedbysearchingthedatabasesofPubMed,EMBASE,andWebofScience.Oddsratios(ORs)with95%confidenceintervals(CIs)wereusedtoassesstheassociation.AlldatawereanalyzedusingStatasoftware.RESULTS:Theassociationbetweenrs641153andAMDriskwasstatisticallysignificantunderthehomozygousmodel(AAvsGG:OR=0.26,95%CI=0.15-0.45,P_h=0.973,/~2=0.0%,fixedeffects),dominantmodel(AA+GAvsGG:OR=0.49,95%CI=0.40-0.59,P_h=0.004,/~2=56.4%,randomeffects)andrecessivemodel(AAvsGA+GG:OR=0.30,95%CI=0.17-0.51,R_n=0.983,I~2=0.0%,fixedeffects).Thesameresultswerealsoobservedinthestratifiedanalysesbyethnicity,sourceofcontrolandsamplesize.CONCLUSION:Ourmeta-analysissuggeststhatrs641153intheCFBgenemayplayaprotectiveroleinAMDsusceptibility,thelateAMDinparticular,bothinCaucasiansandinAsians.
简介:目的:分析丝裂霉素C对青光眼小梁切除术后眼内压和眼血流的影响,探讨其有效性和安全性。方法:将103例青光眼患者随机分为两组,均给予青光眼小梁切除术,观察组在术中行丝裂霉素C抗瘢痕治疗;术后完成6-12mo随访,对比两组患者的临床眼压控制疗效、并发症发生率及视网膜中央动脉血流动力学指标。结果:观察组临床疗效优于对照组(P〈0.05);两组患者的总并发症发生率无统计学差异(P〉0.05),但并发症类型有所区别,观察组多发生低眼压和畏光症状,对照组多发生瘢痕性阻塞。治疗前,两组患者的眼压动力学指标无统计学差异(P〉0.05);治疗后1mo,观察组收缩期峰速(peaksystolicvelocity,PSV)与舒张末期血流速度(enddiastolicvelocity,EDV)明显低于对照组,阻力指数(resistanceindex,RI)与搏动指数(pulsatilityindex,PI)明显高于对照组,差异有统计学意义(P〈0.05);治疗后6mo,观察组PSV与EDV明显高于对照组,RI与PI明显低于对照组,差异有统计学意义(P〈0.05)。结论:丝裂霉素C对青光眼小梁切除术患者的术后不良影响时间较短,其长期疗效安全、可靠。
简介:AIM:Todemonstratethemorphologyandstructureofinvitroreconstructedtissue-engineeredhumancornealepithelium(TE-HCEP)withseedercellsfromanuntransfectedHCEPcellline.·METHODS:TheTE-HCEPswerereconstructedinvitrowithseedercellsfromanuntransfectedHCEPcellline,andscaffoldcarriersofdenudedamnioticmembrane(dAM)inair-liquidinterfaceculturefor3,5,7and9days,respectively.Thespecimenswereexaminedwithhematoxylin-eosin(HE)stainingofparaffin-section,immunocytochemicalstaining,scanningandtransmissionelectronmicroscopy.·RESULTS:DuringinvitroreconstructionofTE-HCEP,HCEPcellsformeda3-4,6-7and8-10layersofanHCEP-likestructureondAMsinair-liquidinterfaceculturefor3,5and7days,respectively.Butthecellsdeceasedto5-6layersandthestructureofstraifiedepitheliumbecamelooseatday9.Andthecellsmaintainedpositiveexpressionofmarkerproteins(keratin3andkeratin12),cell-junctionproteins(zonulaoccludens-1,E-cadherin,connexin43andintegrinβ1)andmembranetransportproteinofNa+-K+ATPase.TheHCEPcellsinTE-HCEPwererichinmicrovillionapicalsurfaceandestablishednumerouscell-cellandcell-dAMjunctionsatday5.·CONCLUSION:ThemorphologyandstructureofthereconstructedTE-HCEPweresimilartothoseofHCEPinvivo.TheHCEPcellsinthereconstructedTE-HCEPmaintainedthepropertiesofHCEPcells,includingabilitiesofformingintercellularandcell-extracellularmatrixjunctionsandabilitiesofperformingmembranetransportation.TheuntransfectedHCEPcellsanddAMscouldpromisinglybeusedinreconstructionHCEPequivalentforclinicalcornealepitheliumtransplantation.