简介:SulfatecanbeactivatedbyATPsulfurylaseandadenosine5-phosphosulfatekinase(APSK)invivo.RecentstudiessuggestedthatAPSKinArabidopsisthalianaregulatedthepartitionbetweenAPSreductionandphosphorylationanditsactivitycanbemodulatedbycellularredoxstatus.InordertostudyregulationofAPSKinrice(OsAPSK),OsAPSKIgenewasclonedanditsactivitywasanalyzed.OsAPSKIC36andC69werefoundtobetheconservedcounterpartsofC86andC119,whichinvolvedindisulfideformationinAtAPSK.C36A/C69AOsAPSK1doublemutationwasmadebysitedirectedmutagenesis.OsAPSKanditsmutantwereprokaryoticallyover-expressedandpurified,andthenassayedforAPSphosphorylationactivity.OsAPSK1activitywasdepressedbyoxidizedglutathione,whiletheactivityofitsmutantwasnot.Furtherstudiesinthecasethatoxidativestresswillfluctuateinvivo3'-phosphoadenosine-5'-phosphosulfatecontent,andallAPSKisoenzymeshavesimilarregulationpatternsarenecessarytobeperformed.
简介:Twostarch-branchingenzyme(SBE)inrice,isknowntobeakeyenzymeinamylopectinbiosynthesis.ThecDNAoftwoSBE(starch-branchingenzyme)genesSheIandShedencodingSBEⅠandSBEⅢ(twomajorisoformsinrice)wereclonedbyanimprovedRT-PCRtechnique,fromatemplatecDNAlibray,derivedfromthetotalmRNAsextractedfromtheimmatureseedsofajaponicariceWuyunjing7.DNAsequenceanalysisshowedthatthesizeoftheclonedSheIandShedcDNAswere2490and2481bplong,respectively,includingtheirentirecodingsequences.ComparisonanalysisindicatedthatthenucleotidesequenceofShe3wasthesameasthatofshed(GenbankAccessionNo.D16201)asreportedpreviously.Therewereonlyfourbase-pairsdifference,whichresultedinchangesoftwodeducedaminoacidsbetweentheclonedShe1cDNAandthereportedshe1(GenbankAccessionNo.D11082).TheclonedSheIandShedcDNAsmakeitpossibletoimprovericestarchqualitythroughgeneticengineering.
简介:Lipoxygenase3(LOX3)isamajorcomponentoftheLOXisozymesinmaturericeseeds.ToinvestigatetheroleofLOX3geneunderstresses,aplantexpressionvectorcontainingantisensecDNAofLOX3wasconstructed.RicevarietiesWuyunjing7andKasalathweretransformedbytheAgrobacterium-mediatedmethodandtransgenicriceplantsweregenerated.PCRandSouthernblotresultsshowedthattheantisenseLOX3genewasintegratedintothericegenome.AnalysesofembryoLOX3deletionandsemi-quantitativeRT-PCRconfirmedtheantisensesuppressionofLOX3geneintransgenicplants.TheT2antisenseplantsofLOX3weresensitivetodroughtstress,riceblastandbacterialblightcomparedwithnon-transgenicplants.TheseresultssuggestthattheLOX3genemightfunctioninresponsetostresses.
简介:TounderstandthewildOryzagenomeeffectonphotosynthesisanditsrelationtototaldrymatteraccumulationinanelitericevariety,asetof40stableintrogressionlines(ILs)BC3F8derivedfromacrossofOryzasativa(KMR3)×Oryzarufipogon(WR120)weregrownunderwellwateredconditions.Leafgasexchangemeasurementsandleafchlorophyllestimateswereconductedatthefloweringstage.Theresultsrevealedsignificantvariationsinnetphotosyntheticrate(Pn),transpirationrate(E),transpirationefficiency(Pn/E)andcarboxylationefficiency(Pn/Ci).PnshowedsignificantpositivecorrelationwithE,stomatalconductance(gs),Pn/Ciandtotalcanopydrymatter.SpecificleafareaandleafthicknesswerenotsignificantlycorrelatedwithPn.Thirty-sevenoutof40ILsshowedhigherPnthanKMR3[11.28μmol/(m2·s)],and20ILsshowedhigherPnthanWR120[15.08μmol/(m2·s)].ThelineIL194showedthehighestPn[21.62μmol/(m2·s)]withincreasedtotalcanopydrymatterfollowedbylinesIL381,IL106,IL363-12,IL198,IL86-18andIL50,whichexhibitedPnabove18.0μmol/(m2·s).TheILswithenhancedPnareapotentialsourcefordevelopingricevarietiesandhybridswithhigherbiomassandyield.
简介:TheinteractionbetweenricehostanditspathogenXanthomonasoryzaepv.oryzae(Xoo)atcellularlevelwasstudiedbyusingaresistantsomaclonalmutantHX-3anditssusceptabledonorMinghui63.AfterinoculationwithXoostrainZhe173(Chinesepathotype|\).theactivityofsuperoxidedismutase(SOD)andperoxidase(POD)inthecallusofMinghui63wasincreaseddramatically,andtheactiveoxygen(O2^-)wasproducedatahigherrate;Meanwhile,thecallusgrewslowlywiththereductionofproteincontent.ComparedtotheactivityofSODandPOD.theproductionrateofO2^-andthefreshweightinHX-3callusvariedlittleaftertheinoculation.ItcouldbeproposedthatthereweregreatdifferencesbetweentheresistanceofHX-3andMighui63atcellularlevel.TherewasnodifferencedetectedconcerningresistancetobacterialleafblightinHX-3betweentheplantandthecallus.
简介:到精选精英germplasms,从装饰用的梨树米饭栽培变种Wuyujing3导出的112异种被评估。象圆锥花序那样的收益部件每平方米数,谷物数字每圆锥花序,和谷物,重量被测量。象白垩的谷物(PCG)的百分比那样的优秀特点,稻谷收益(BRY),milled米饭收益(MRY),milling(DM)的度,直链淀粉内容(交流),蛋白质内容(PC),和在特点之中的关系是inverstigated。结果证明谷物产量每在谷物收益变化为94.64%贡献的平方米与6.4t/hm2的一个平均数和谷物的数字从2.15~12.49t/hm2。为优秀特点,所有米饭异种有短尺寸(谷物长度≤5.5 ;公里)并且大胆形状(到宽度比率=1.10-2.00的谷物长度)。大多数米饭异种(87.5%)低于20%有PCG价值。有的MRY重视甚于50%的所有异种,低于20%的交流值,和低于10%的PC值。白垩的谷物的百分比否定地显著地与MRY被相关并且断然与DM相关。BRY和MRY否定地显著地与DM被相关。PC显著地并且断然与MRY被相关并且否定地与DM相关,当交流没这些优秀特点地有重要关联时。有25米饭异种,完成了象白垩的谷物的低百分比那样的江苏标准装饰用的梨树米饭的主要要求,这被结束,低直链淀粉满意的、最佳的蛋白质内容,并且它能被用作精英germplasms。因此,识别的异种可以在米饭质量的改进导致重要进步。
简介:为了推进,改进高地大米变化Huhan3和Huhan7,种子样品为约1和5d与二艘可重获的飞船被送到外层空间并且分别地为7和5代被宣传。Phenotypic分析表明词法特点和蛋白质和谷物的直链淀粉内容变化了。由联系基因的简单顺序重复(SSR)和插入删除(InDel)的genomic变化的描述标记显示变化模式是很复杂的。大多数变化在简单顺序重复碎片发生在碎片的3结束或5结束。反向的抄写聚合酶链反应(RT-PCR)试金证明在SSR的那些部分的变化影响了他们的基因表示,显示那基因联系了标记将有用孤立功能的基因。为繁殖的地调查也表明有高产量,高质量和干旱忍耐的更多的线能通过太空繁殖被选择。结果显示太空mutagenesis为米饭繁殖导致了分子的变化,以及生理、词法的变化。
简介:Themajormatesterilegenesinanewphoto/thermo-sensitivegenicmalesterile(PTGMS)lineB06Sofricewereanalyzedbythemanipulationofmixturedistributiontheory.TheresultsindicatedthatapairofmajormalesterilenucleargeneswithlargeeffectswereresponsibleforcontrollingthemalesterilityofB06S.
简介:四十个SSR标记被用来在在1950年代并且在种的151个中国主要米饭变化比较基因差异变化最近十年。40SSRloci,39被发现当时,多态一地点(RM479)monomorphic。213等位基因的一个总数从39多态的loci被识别。等位基因的平均数字每地点(Na)具有5.5,从2~11。Nei的基因差异索引(他)在RM418从0.309atRM174在loci之中急速地变化了到0.869,与0.649的平均值。在在indica和装饰用的梨树亚种之间的SSRallelic差异的在那里存在的重要差别,和indica在Na两个都比装饰用的梨树有更多的变化并且他。由有在Na的基因变化的比较并且他,在1950年代种的变化有更多的等位基因,这被揭示并且更高他比那些在里面最近为indica的十年和装饰用的梨树米饭。为Na的二亚种之间的差别随着时间的过去在一个趋势是重要的(indica:z=2.677,P=0.007;装饰用的梨树:z=3.441,P=0.001),然而并非为重要他(indica:z=1.471,P=0.141;装饰用的梨树:z=1.932,P=0.053)。分子的变化(AMOVA)的分析显示了那在那里存在的重要差别(P<0.05)在在二个时期,更多的遗传变异被indica(Fst=0.050)和装饰用的梨树贡献之间的遗传变异(Fst=0.082)子集。用locus-by-locusAMOVA过程,重要基因区别为indica变化和11loci在13loci(RM21,RM128,RM147,RM169,RM190,RM221,RM231,RM251,RM253,RM317,RM341,RM418,和RM478)被观察(RM101,RM135,RM152,RM159,RM169RM190RM251RM253RM311,RM418,和RM478)为在二时期之间的装饰用的梨树一。Itwas发现一些等位基因作为与那些in1950s作比较沉醉于当前的主要米饭变化。因此,利用更多的相异的精英应该是必要的为在当前的米饭繁殖的遗传背景的扩展的基因资源编程序。
简介:Twonewlybredhybridricecombinations,superhigh-yieldingLiangyoupeijiu(Pei'ai64S×9311)andPei'ai64S/E32(Pei'ai64S×E32)wereusedtoinvestigatethephotosyntheticcharacteristicsunderhightemperatureincomparisonwithhybridriceShangyou63.Hightemperaturecausedadecreasedphotosyntheticefficiencyandaggravatedphotoinhibition.TheoptimumtemperatureforphotosyntheticelectrontransportationandphotosyntheticCO2fixationwereabout28℃and35-40℃respectively.Linearelectrontransportationismoresensitivetohightemperaturethanthephotochemicalprocess.Themechanismofhightemperatureadaptationwaspossiblyasfollows:superhigh-yieldingricehasquicklyincreasingcarotenoid,whichactedasamorefavorableantioxidantsystemtoreducetheactiveoxygenproductionandavoiddamagetothephotosynthesissystem;superhigh-yieldingricehasahigherefficiencyofxanthophyllscycletodissipateexcessheatenergy;superhigh-yieldingricehasamorestablephotosyntheticfunction,higherphotosyntheticefficiencyandmoreheatstableproteincontent.
简介:布朗planthopper(Nilaparvatalugens圣?l)大多数损坏害虫之一在米饭正在引起hopper灼伤,并且从而减少生产率并且另外产品的质量。控制这个害虫的有效管理策略是到本地米饭栽培变种的理想的基因的鉴定和转移。为开发抵抗栽培变种的最重要的途径是标记的鉴定,它能在更持久的抵抗遗传型的帮助标记的选择帮助。易受影响的父母IR50和抵抗父母Ptb33,和他们的F2人口是为有随机的放大多态的DNA的抵抗基因的鉴定的使用的inbulkedsegregant分析标记(RAPD)教材。教材OPC7和OPAG14证明主导、易受影响的特定的banding模式那么叫了co主导的标记。而且,OPC7697和OPAG14680给抵抗特定的乐队看了并且因此在联合分阶段执行,而OPC7846和OPAG14650给易受影响的特定的genotypic乐队看了inbulkedsegregant分析。因此,联合阶段标记,OPC7697和OPAG14680,被认为在在庄稼改进的米饭遗传型的帮助标记的选择更有用。