简介:Inrecentyears,heavyionbeamshavebeenrecognizedasaneffectiveandefficientphysicalmutagenduetotheircapacitytoinducemutationswithhighfrequencyandbroadspectrum.Nowadays,itisnotsodifficulttoproducevariousmutantsinplants.However,tominetheresponsiblemutatedgeneshasbeenanimportantchallenge.Formutationisolation,map-basedcloningisoneofthemajortraditionalwaystoisolatethemutantgenesthatcontroltraitsofinterestinforwardgeneticsstudies.However,theprocessofmap-basedcloningisusuallycomplicatedandtime-consuming.