简介：TheSOD-likeactivityoffiveCu(Ⅱ)complexes[Cu(HSal)_2·EtOH,Cu(Gly)_2,Cu(Lys)_2,Cu(His)_2,Cu(Try)_2]havebeenstudiedbyusingcytochromeCmethod,aswellaschemilumine-scenceandESRtechnique.Thefoursurfactants(CTAB,SDS,TritonX-100andTween20)werepurifiedandtheircriticalmiceileconcentration(CMC)inphosphatebuffer(pH=7.4)wasmea-sured.TheeffectsoffoursurfactantsonO_2~-havebeeninvestigated.Theco-operativeeffectsof

简介：CyclicADP-ribose(cADPR)isauniversalCa2+mobilizingsecondmessengerinmanydifferentcelltypesandorganisms.cADPRactivatesCa2+releasefromendo/sarcoplasmicreticulumviaryanodinereceptors.Inaddition,Ca2+entrysecondarytoCa2+depletionisatleastoneofthemechanismsinwhichcADPRtriggersCa2+inflow,too.AnaloguesofcADPRhavebeenpreparedbychemicalandchemo-enzymaticroutes.MostoftheanalogueswereanalyzedforbiologicalactivityinintactorpermeabilizedJurkatTcells(ahumanT-lymphomacellline).Asasystematicapproach,analoguesweregroupedaccordingtoalterationsinthebase,thenorthernribose,thesouthernribose,thepyrophosphatebackbone,orincomplexmodifications,comprisingmorethanonepartofthemolecule.Biologicalactivityoftheanaloguesisreviewed,withspecialemphasisonJurkatTcells.

简介：TheSOD-likeactivitiesofcoppersalicylateencapsulatedinneutralpositivelyandnegativelychargedliposomeswerestudiedwithcytochromecmethod.TheresultsshowedthattheSOD-likeactivityofcoppersalicylateencapsulatedinneutralliposomeswasenhancedover3timesmorethanthatofcoppersalicylateinaqueoussolution.However,theactivitydecreasedforcoppersalicylateencapsulatedinpositivelyandnegativelychargedliposomes.Itwasalsofoundthatatexperimentalconcentrationstherewasnoregularityforthechangeofactivityofcoppersalicylateencapsulatedinnegativelychargedliposomes,andcoppersalicylateencapsulatedinliposomeswithmorenegativechargescouldpromotethedismutationofsuperoxideanionfreeradicals,however,socouldcoppersalicylateencapsulatedinliposomeswithfewernegativechargesonlyinlowerconcentrations.

简介：Objective:Tostudytheanti-inflammatoryactivityandtissuedistributionpatternsofintravenousemulsionofdexamethasoneacetateinmice.Methods:Theanti-inflammatorysolutionforinjectionandLimethasone(Jepaneseproduct)givenintravenouslywereevaluatedbyusingthepreformedcarrageenangranulomapouchmethodinrats.Results:Theanti-inflammatoryactivityofdexamethasoneacetateemulsionatlowdoseof0.05mg.kg1wasaspotentasdexamethasonesodiumphosphatesolutionathighdoseof0.3mg.kg1.Thedistributionpatternsinmicetissuesof[^3H]dexamethasoneacetateemulsionand[^3H]dexamethasonesodiumphosphatesolutioninmiceweremarkedlydifferent.Dexamethasoneacetateemulsionshowedamuchhigherconcentrationintheliver,spleen,lung,andinflamedtissues,whereasdexamethasonesodiumphosphatehadahighconcenti,moninthemusclesofvastuslateralis.Theseresultsmayindicatethatdexamethasoneincoporatedinlipidemulsionwastakenupbythereticuloendothelialsystemandinflammatorycellsmuchmorethandexamethasonesodiumphosphatesolution.Conclusion:Whendexamethasoneacetatewasincorporatedinemulsion,thedistributionpatternsintissueswerechangedandtheyhadastrongeranti-inflammatoryactivity.

简介：AIMTostudytheeffectofketoconazole(KTZ),aselectiveinhibitorofCYP3A,oninvivoandinvitrometabolicactivityofhepaticCYP3Ainratwithmidazolam(MDZ)asprobe,whichwasassessedbypharmacokineticparametersofMDZ.,andtoestablishasuitablemarkerorindicatorforestimatingdrugmetabolizingactivityofhepaticCYP3A.METHODS1.Invivostudy:SeveralloadingdosesofKTZpreparedinamixtureofPEG400andpropyleneglycol(9:1)wereadministratedthroughratsublingualveinfollowedbyconstantinfusionatdifferentratesthroughtailveinwithanattempttoachievecorrespondingsteady-stateplasmaconcentrationsinordertoattaincontinuousinhibitiononCYP3A.

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简介：实验表明给小鼠五灵脂提取液，小鼠血液ＳＯＤ活性增强。测定五灵脂有类ＳＯＤ活性，在ｐＨ４５～８５，温度２５～１００℃条件下活性稳定

简介：Objective:ToexplorethemechanismofEcdysterone(ECR)inpreventionoflearningandmemorydysfunctionoftheratsinducedbyβ-amyloidpeptide(Aβ25-35).Methods:NinetyWistarmaleratswererandomlydividedintofivegroups,thecontrolgroup,themodelgroup,thetreatedgroups(ECR4mg·kg^-1andECR8mg·kg-1andNimodipine

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简介：AIMMacrophagemigrationinhibitoryfactor(MIF)isapro-inflammatorycytokineinvolvedinthepathogenesisofavarietyofautoimmuneandinflammatorydiseases,includingarthritis,glomerulonephritis,Gram-positiveandGram-negativesepsis,andatherogenesis.RecentstudiesshowedthatCD74(antigen-associatedinvariantchainⅡ)isahigh-affinitymembrane-bindingproteinforMIF.ThepurposeofthepresentstudywastoexpresstherecombinanthumanCD74inE.coliandinhibittheactivityofMIFbyusingrecombinantCD74invitro.

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