简介:ObjectivesTostudyclinicalandcoronaryangiographicfindingsinpatientswithbothcoronaryheartdiseases(CHD)andtype2diabetesmellitus(T2DM).Methods215patientswithCHDconfirmedbycoronaryangiographywereinvolvedinthisstudy.Thepatientsweredividedintotwogroups:74CHDpatientswithT2DM(meanage64.7±8.2years,male/female47/27),and141CHDpatientswithoutT2DM(meanage66.2±9.2years,male/female100/41).Theclinicalfeaturesandthedatafromselectivecoronaryangiographieswerecomparedbetweentype2diabeticandnon-diabeticCHDpatients.ResultsComparedtonon-diabeticCHDpatients,thepatientswithbothCHDandT2DMsufferedmorefromacutemyocardialinfarction,silentischemiaandseverearrhythmias(P<0.01,P<0.05),andhadhigherserumtriglyceridesandapo-lipoproteinB,alongwithincreasedserumuricacid(P<0.01,P<0.05),increasedleftventricularenddiastolicdiameter(P<0.01),anddecreasedleftventricularejectionfraction(P<0.001).Comparedtonon-diabeticCHDpatients,thepatientswithbothCHDandT2DMsufferedmorefromtriplevesseldisease(P<0.01),severecoronaryarterystenosis,completeocclusionsanddiffuselesions(P<0.001).ConclusionsSevereclinicalmanifestation,leftventriculardysfunction,diffuseorcomplicatedlesionsofcoronaryarteriesweremorecommoninpatientswithbothCHDandT2DM,itsuggeststhatthetype2diabeticCHDpatientshavepoorprognosis.
简介:Themyocardialprotectionaffordedbyischemicpreconditioning(IPC)canalleviateischemia-reperfusioninjuryinnormalratheart.However,thismyocardialprotectionisseldomstudiedinthetype2diabeticratwithmyocardialischemiadisease.Inthisstudy,weaimedtoevaluatetheeffectsofATP-sensitivepotassiumchannels(KATPchannels)onIPCintheisolatedtype2diabeticratheartandtheroleofthesulfonylureagliclazide.MethodsStreptozotocin(STZ)-inducedtype2diabeticmaleWistarratswithorwithoutgliclazide(64mg/kgbodyweight,orally)andage-matchednon-diabeticcontrolratswereusedforallstudies.TheisolatedheartswereperfusedwithLangendorff'ssystemundertheconstantflow,pressureandtemperatureconditionswithKreb's-Henseleitsolution(K-H).After5minutesofbalanceperfusion,theseratswererandomlydividedintosixgroups:non-diabeticcontrolratswithoutIPC(CIR);non-diabeticcontrolratswithIPC(CIP);diabeticratswithoutIPC(DIR);diabeticratswithIPC(DIP);gliclazide-treateddiabeticratswithoutIPC(GIR);andgliclazide-treateddiabeticratswithIPC(GIP).GroupsCIR,DIR,andGIRweresubjectedto30-minglobalischemiaand60-minreperfusionforinductionofischemia/reperfusioninjury.GroupsCIP,DIP,andGIPweregiventhreecyclesof5-minischemiaand5-minreperfusionasIPC,andthenischemia/reperfusioninjuryprogramwasimplemented.Extentofischemia/reperfusioninjurywasmeasuredintermsofthereleaseoflactatedehydrogenase(LDH),creatinekinase(CK),andcreatinkinase-MB(CKMB)incoronaryeffluent.Afterperfusion,Kir6.2andSUR2AmRNAexpressionsinthemyocardialtissuewerecharacterizedbyfluorescentquantitativereal-timePCRmethod,andKir6.2andSUR2Aproteinexpressionswereassessedbyimmunohistochemistry.ResultInnon-diabeticcontrolrats,thereleaseofLDH,CK,andCK-MBincoronaryeffluentmarkedlydecreasedwithIPCcomparedwithNo-IPC(P<0.05),butnotindiabeticrats.However,ingliclazide-treateddiabeticrats,IPC-induceddecreaseintherele
简介:ObjectivesTheeffectsofcarvediloloncalciumcurrent(ICa)wereinvestigatedinisolatedadultratventricularmyocytes.MethodsICawasrecordedbyusingwhole-cellpatch-clamprecordingtechnique.ResultsCarvedilolreversiblyinhibitedICainaconcentration-dependentmanner,carvedilolat0.1,0.3,1and10μmol/LintheextracellularsolutiondecreasedpeakICaby1.52%,18.04%,37.34%and72.18%,respectively.Thesteady-stateinactivationcurveofICawasshiftedtomorenegativepotentials,whiletheactivationcurvewasnotaltered.Therecoveryfrominactivationwasshiftedtorightdirection,itcouldnotberecoveredcompletely.Inaddition,Pretreatmentofventricularmyocyteswithprazosinandpropranololcouldn'tblockthecarvedilol-inducedreductionofICa.ConclusionsCarvedilolinhibitsICainadultratventricularmyocytesbymechanismsinvolvingpreferentialinteractionwiththeinactivatedstateofcalciumchannel.
简介:目的:探讨2型糖尿病(T2DM)合并高血压与否对脂代谢、微血管病变、血液流变学的影响。方法:192例T2DM患者被分为血压正常组(A组,96例)和合并高血压组(B组,100例),对两组的一般情况、血压、脂代谢、微血管损害及血液流变学情况进行比较分析。结果:B组的血压(148±24)mmHg/(86±15)mmHg、胆固醇(6.0±0.9)mmol/L、血肌酐(70±21.1)μmol/L、血尿酸(343±81)μmol/L和低切粘度(11.02±2.3)均高于A组,有显著性差异(P〈0.05-〈0.01)。结论:T2DM和高血压病均可导致血脂代谢异常,微血管损害和血液流变学的改变,当T2DM合并高血压时,前述异常和损害加重,应及早控制。
简介:目的对中西医结合治疗在2型糖尿病患者中的治疗方式进行讨论。方法选取我院2013年2月-2014年2月间64例2型糖尿病患者,将其分为两组,1组进行常规治疗,2组在进行常规治疗的同时配合中药治疗,对两组患者的治疗效果进行比较。结果1组患者治疗后效果显著的为13例,占比例的40.6%,治疗有效的为14例,占比例的43.8%,治疗无效的患者为5例,占比例的15.6%。2组患者治疗效果显著的为23例,占比例的71.9%,治疗有效的为8例,占比例的25%,治疗无效的仅为1例,占比例的3.1%。结论中西医结合方式治疗2型糖尿病的效果显著,且安全性高,是一种值得推广的治疗方式。
简介:ObjectivesToevaluateantihypertensiveefficiencyandsafetyofanewdomesticofL-&N-typeCa^2+antagonist-eilnidipinewithimidaprilasapositivecontrol.MethodsAfter2weeks'placebowashingout,22patientsweretreatedwitheilnidipine5mgdailyand27patientsweretreatedwithimidapril5mgdaily.4weekslater,ifpatient'ssittingdiastolicbloodpressureisover90mmHg,his/herdosagewasdoubledforanother4weeks,theothersmeasuringupremainedtheirdosageunchangedforanother4weeks.Bloodpressure,heartrate,bloodandurineroutineexamination,serumglucose,serumchemicalexaminationincludingtotalcholesterol,triglyceride,HDL,LDL,transaminase,creatineetcandsidereactionswererecordedbeforeandafterthetrial.Datawereanalyzedstatistically.ResultsAfter8weeks'treatment,bloodpressurewassignificantlydecreased(P<0.05)inbothgroups,andthetwomedicineshadsimilarantihypertensiveeffects.Furthermore,thereducingofheartratewasstatisticallysignificantcomparedwithbaseline(P<0.01)inthecilnidipinegroup,butnotintheimidaprilgroup.Thenegativechronotropiceffectofcilnidipinehadlittleeffectoncontinuingthetherapy.Therewerenochangesonbloodandurineroutineexaminationandserumlipid,serumglucose,creatine,transaminaseandetcinbothgroups.Theirsidereactionsweremildandwell-tolerated.ConclusionsCilnidipinehasacon-vincingantihypertensiveeffectsimilartothatofimi-dapril.Especiallycilnidipinemaybeadministeredtopatientswithrelativelymildtachycardia.
简介:目的观察脑出血后LINGO-1表达的变化,探讨维甲酸对脑出血后LINGO-1表达的影响。方法将72只SD大鼠随机分为假手术组、模型组和维甲酸治疗组,选取造模后1d、3d、7d和14d为观察点。制备脑出血模型,Longa评分法评价神经功能缺损程度,RT-PCR法检测LINGO-1mRNA的表达,Westernblot法检测LINGO-1蛋白的表达。结果模型组Longa评分7d最高,14d出现下降;LONG-1mRNA3d表达最高,7-14d出现下降;LONG-1蛋白7d到高峰,14d出现下降。维甲酸治疗组在14dLonga评分较模型组下降(P〈0.05);在7d和14dLINGO-1mRNA表达较模型组下降(P〈0.05);在14dLINGO-1蛋白表达较模型组下降(P〈0.05)。结论脑出血后LINGO-1表达明显上调,呈先上升后下降的变化规律。维甲酸可以降低LINGO-1mRNA和蛋白的表达及神经功能评分。
简介:Thestudyaimedtoinvestigatetheeffectsofivabradineonthelevelsofhypoxia-induciblefactor-1alpha(HIF-1α)andVEGFinserumofrabbitwithacutemyocardialinfarction(AMI).MethodsAMImodelwasestablishedbyligatingtheleftanteriordescendingbranchofthecoronaryarteryinNewZealandwhiterabbits.Twentyfiverabbitswererandomlydividedinto4groups:sham-operated(S),myocardial-infarction(M)withbisoprololtreatment(M+B)andivabradine-treated(I+M).Themedicaltreatmentbeganimmediatelyafterinfarctionandcontinuedfor3weeks.Serumofeachrabbitwasobtainedatthefollowingtimepoints(24hbeforetheoperation,24h,3d,1week,2weeksand3weeksaftertheoperation).ELISAwasusedtomeasurethelevelsofHIF-1αandVEGFofeachsample.ECGandheartrates(beforeandaftertreatment)wereanalyzed.ResultsBaselineheartrateshowednosignificantdifferencesbetweenthe3infarctedgroups(M,M+B,M+I).ThreeweekslatertheheartratesweresignificantlyloweringroupM+BandgroupM+IthaningroupM.However,therewasnostatisticdifferencebetweenthetwodrug-treatedgroups(P=0.848).ThelevelsofHIF-1αandVEGFingroupsM,M+BandM+I)increasedsignificantlycomparedwithgroupS(P<0.01).TheproductionsofHIF-1αandVEGFwereloweringroupM+BandgroupM+IcomparedwithgroupM(P<0.01).TherewasnostatisticaldifferencebetweenthegroupM+BandgroupM+I(P>0.05),andthecorrelativeanalysisrevealedthattheproductionofHIF-1αwaspositivelycorrelatedwiththatofVEGF(r=0.732,P<0.01).ConclusionIvabradinecanreduceheartrateandmeanwhiledecreasetheserumlevelsofHIF-1αandVEGFafterAMI.