简介：摘要：人教版高中化学教材以“继承·发展·创新”为指导思想，彰显化学的育人价值。本文以普通高中化学教材必修第一册为研究对象，主要对教材中栏目设置的情况以及特点进行思考研究。19人教版化学教材的栏目设置可以分为六类：思考交流类、实验探究类、课外阅读类、方法指引类、归纳运用类、化学与STSE类。

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简介：摘要：目前在我们国家，院前急救工作一直没有得到社会的重视，更没得到广大人民群众的认可，更甚至有非常一大部分让你都不知道有120调度指挥这个工作，更不曾谈及了解急救越早开始越有希望的意义。希望通过这篇文章让社会了解什么是院前急救调度，调度员是干什么工作，调度工作对于抢救病人生命有多大重要的意义。了解现代医学的抢救病人生命的模式，只有把抢救工作提前到当下立即开始才是最重要的。希望社会上更多的人认识到真正的的急救是从调度员接通电话的瞬间开始的而不是救护车到才开始的。大家共同努力才能更好的挽救每一个垂危的生命。

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简介：【摘要】目的：对我院某一段时间院前急救调度的信息进行分析，总结特点，为提升院前急救水平和指挥调度质量提供参考。方法：将我院在2019年-2020年“120”指挥调度的信息资料进行分析，主要统计呼叫疾病的种类、呼叫高峰时间段等。结果：经过统计可知，120院前急救呼救最多为交通事故上，其次是内科疾病、心脑血管疾病和其他种类疾病；呼救最高的时间短是在18:00-24:00。结论：在院前急救指挥调度中，交通事故是常见的呼救原因，晚间和夜间是呼叫的高峰时间段。医院要根据这个特点来进行调度，在高峰时期要增加急救相关人员与物品的准备，提升急救水平，确保指挥调度的质量。

简介：[摘要]朴素大方和端正谦和的矩形形态于中国传统文化机缘巧合地紧密相连，在中国古代造物中诠释着中国先人的中庸思想、宗法思想和朴素宇宙观，充满了浓厚的东方设计色彩，一直影响着中国传统设计的发展方向。

简介：摘要目的分析复发/难治性急性B淋巴细胞白血病（R/R B-ALL）自体CD19嵌合抗原受体T细胞（CAR-T细胞）制备过程中，培养体系中残留白血病细胞导致CD19 CAR转入白血病细胞的特征和体外杀伤研究。方法①收集30例接受CD19 CAR-T细胞治疗的R/R B-ALL患者及6例健康志愿者外周血单个核细胞（PBMC）；②流式细胞术分析R/R B-ALL患者PBMC经CD3磁珠分选后及体外培养过程中体系白血病细胞残留情况；③患者及健康志愿者PBMC CD3+ T细胞转染CD19 CAR及CD22 CAR慢病毒，制备CD19 CAR-T、CD22 CAR-T细胞；④复苏Nalm-6细胞株，CD19 CAR慢病毒转染Nalm-6细胞，制备CD19 CAR-Nalm-6细胞，同时转染患者原代ALL细胞；⑤流式细胞术检测转染率；⑥CCK-8法检测细胞增殖；⑦乳酸脱氢酶（LDH）法检测CD19 CAR-T、CD22 CAR-T细胞对Nalm-6细胞及CD19 CAR-Nalm-6细胞杀伤活性。结果①30例接受CD19 CAR-T细胞治疗的R/R B-ALL患者中，2例CD3+ T细胞中发现3.32%、2.04%的白血病细胞残留，随体外培养时间延长，在培养第4天，白血病细胞完全消失。②体外培养中CD19 CAR-Nalm-6细胞增殖率高于Nalm-6细胞。③效靶比为1∶1且共培养24、48、72 h，CD19 CAR-T细胞对Nalm-6细胞的杀伤活性高于CD19 CAR-Nalm-6细胞；与CD19 CAR-T细胞相比，CD22 CAR-T细胞对CD19 CAR-Nalm-6细胞的杀伤活性更高。④相同效靶比情况，单独应用CD22 CAR-T细胞对CD19 CAR-Nalm-6细胞的杀伤活性高于CD19 CAR-T联合CD22 CAR-T细胞。结论CD19 CAR-T细胞制备过程中培养体系残留白血病细胞可能会导致CD19 CAR被引入白血病细胞中而导致CD19 CAR-T细胞治疗失败，在细胞制备过程中需要检测培养体系中白血病细胞的残留情况。CD22 CAR-T细胞治疗可作为上述情况的挽救治疗措施之一。

简介：摘要目的分析复发/难治性急性B淋巴细胞白血病（R/R B-ALL）自体CD19嵌合抗原受体T细胞（CAR-T细胞）制备过程中，培养体系中残留白血病细胞导致CD19 CAR转入白血病细胞的特征和体外杀伤研究。方法①收集30例接受CD19 CAR-T细胞治疗的R/R B-ALL患者及6例健康志愿者外周血单个核细胞（PBMC）；②流式细胞术分析R/R B-ALL患者PBMC经CD3磁珠分选后及体外培养过程中体系白血病细胞残留情况；③患者及健康志愿者PBMC CD3+ T细胞转染CD19 CAR及CD22 CAR慢病毒，制备CD19 CAR-T、CD22 CAR-T细胞；④复苏Nalm-6细胞株，CD19 CAR慢病毒转染Nalm-6细胞，制备CD19 CAR-Nalm-6细胞，同时转染患者原代ALL细胞；⑤流式细胞术检测转染率；⑥CCK-8法检测细胞增殖；⑦乳酸脱氢酶（LDH）法检测CD19 CAR-T、CD22 CAR-T细胞对Nalm-6细胞及CD19 CAR-Nalm-6细胞杀伤活性。结果①30例接受CD19 CAR-T细胞治疗的R/R B-ALL患者中，2例CD3+ T细胞中发现3.32%、2.04%的白血病细胞残留，随体外培养时间延长，在培养第4天，白血病细胞完全消失。②体外培养中CD19 CAR-Nalm-6细胞增殖率高于Nalm-6细胞。③效靶比为1∶1且共培养24、48、72 h，CD19 CAR-T细胞对Nalm-6细胞的杀伤活性高于CD19 CAR-Nalm-6细胞；与CD19 CAR-T细胞相比，CD22 CAR-T细胞对CD19 CAR-Nalm-6细胞的杀伤活性更高。④相同效靶比情况，单独应用CD22 CAR-T细胞对CD19 CAR-Nalm-6细胞的杀伤活性高于CD19 CAR-T联合CD22 CAR-T细胞。结论CD19 CAR-T细胞制备过程中培养体系残留白血病细胞可能会导致CD19 CAR被引入白血病细胞中而导致CD19 CAR-T细胞治疗失败，在细胞制备过程中需要检测培养体系中白血病细胞的残留情况。CD22 CAR-T细胞治疗可作为上述情况的挽救治疗措施之一。

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简介：摘要：国有企业纪委必须保持正风肃纪高压态势，严肃查办各类违规违纪案件，同时认真做好查办案件“后半篇文章”，这是不断提高国有企业党员干部纪法意识，弘扬党内政治文化、构建健康政治生态的重要路径。不仅要以案促改，还应做到促教、促建。应当在典型案例当中吸收经验教训，规范管理流程，堵塞管理漏洞，最大限度提升国有企业的经济效益。本文将对查办案件综合功能发挥的有效途径展开积极探索。

简介：摘要：学语文教师在教学中要为学生设计课后练习。教师要通过引导学生进行练习来激发学生的学习兴趣，全面反思学过的知识，结合自己的学习情况自主完成学习项目。当学生优化了自己的练习以后，教师就能以此提高课堂教学的效率。

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简介：摘要：地表水环境监测是在遵循水循环发展的基本规律上，调查水体质量，防治水污染，保护地表水水质，保障人体健康，维护良好的生态系统。本文以亳州市为例，对基层地表水环境监测短板进行客观分析，就如何提高地表水监测质量管理，扎实做好监测垂改后半篇文章，提出一些建议供参考。

简介：摘要：站在学生“学”的层面，基于文体、基于文本特点来考虑、组织写人类文章的问题导学策略，还给学生学的权力、时间、乐趣，让学生在主动、自主、真实的学习过程中获得知识，形成能力，最终达到提高语文教学实效、提升学生语文素养这一终极目标。

简介：摘要：为了有效确保各个医疗机构遵纪守法、规范运营，杜绝各种违法乱纪的先行者，多个省市都积极出台了相关医疗保障创新管理的意见和政策。要求各医疗机构需要创新管理服务模式，将传统的监管和社会信用体系进行融合，并制定政务服务、信用评价、行政处罚等措施，以此构建基于信用+”的医保基金监管新模式，打造新格局，确保各个医疗机构医保基金的高效、稳定、长久运行。在此基础上医疗机构和药店都需要明确自身职责，签订医保信用承诺书，提高自身的信用水平，以此科学落实以上工作。

简介：AbstractObjective:To investigate and analyze changes of T lymphocyte and other lymphocyte subsets in the peripheral blood of patients with coronavirus disease 2019 (COVID-19), with the goal of improving clinical understanding and the value of research applications.Methods:General data of 66 confirmed COVID-19 patients admitted to the Fifth Medical Center of Beijing PLA General Hospital from January 2 to March 23, 2020 were collected in this retrospective case-control observational study, and they were divided into mild (n=26), mid-grade (n=19), and severe/critical disease groups (n=21) according to disease severity. Neutrophils, lymphocytes, neutrophil/lymphocyte ratios, CD4 absolute counts, CD8 absolute counts, and CD4/CD8 expression ratios of peripheral whole blood among the three patient groups were compared. The study protocol was approved by the Ethics Committee of the Fifth Medical Center, General Hospital of Chinese PLA (approval No. 2020-69-D) on May 5, 2020.Results:Among the 66 COVID-19 patients examined, 38 were male and 28 were female, with an average age of 53 ± 17 years. Among patients, 26 cases were mild, 19 cases were mid-grade, and 21 cases were severe/critical. Neutrophils, neutrophil/lymphocyte ratios, and CD4+/CD8+ ratios of the severe/critical group were significantly higher compared with mild and mid-grade groups (P < 0.01); however, there was no obvious difference between mid-grade and mild groups (P > 0.05). Lymphocytes, CD4 absolute counts, and CD8+ absolute counts of the severe/critical group were significantly lower compared with mild and mid-grade groups (P < 0.01); however, there was no significant difference between mid-grade and mild groups (P > 0.05).Conclusion:Counts of lymphocytes and T lymphocytes in severe/critically ill patients were decreased, which is of great significance for the identification of severe and critical COVID-19 patients.

简介：AbstractPurpose:As COVID-19 spreads globally and affects people's health, there are concerns that the pandemic and control policies may have psychological effects on young people (age from 17 to 35 years). This psychological impact might vary in different countries, and thus we compared the prevalence of self-reported psychological distress, loneliness and posttraumatic stress symptoms (PTSS) among young people in the United Kingdom (UK) and China at the beginning of the COVID-19 pandemic.Methods:Data of this study came from two sources. One source was the first wave of COVID-19 study in Understanding Society, a special wave of the UK household longitudinal study, which provided the high-quality, national-wide representative panel data. The sample comprised 1054 young people. The other source was an online survey on the mental health of 1003 young people from Shanghai, a highly developed area in China. The questionnaire included questions on the prevalence of common mental disorders (cut-off score ≥ 4), loneliness and potential PTSS (cut-off ≥ 33). Univariable analyses were conducted to test the differences in the self-reported prevalence of psychological distress and loneliness between the two groups. Multivariable logistic regression analyses were run to explore the predictors of psychological distress and loneliness among all the young people from England and Shanghai.Results:Among the samples with self-reported psychological distress, the UK sample accounted for 34.4% (n=1054) and the Chinese sample accounted for 14.1% (n=1003). The difference between the two groups was statistically significant (p < 0.001). Additionally, 57.1% of people in the UK and 46.7% in China reported that they sometimes or often felt lonely, of which the difference is statistically significant (p < 0.001). Regression analysis of the entire samples showed that nationality, gender, psychotherapy and loneliness were significant predictors of 12-item General Health Questionnaire scores, while the variables of age and living alone were not. Significant predictors of self-reported loneliness were the nationality, gender, age, living alone and psychotherapy. In China, 123 (12.3%) young people, 49 men (11.3%) and 74 women (13.0%), met the criteria of PTSS symptoms (cut-off scores ≥ 33). These scores were only collected in China.Conclusion:This evidence suggests that mental health and loneliness reported by young people were lower in China than that in the UK during the studied period. More research is needed to understand these differences. If the differential negative psychological impacts are confirmed, country-specific measures of prevention and intervention should be adopted to improve the mental health of young people under the ongoing impact of the pandemic.

简介：AbstractObjective:Emetine, an isoquinoline alkaloid that is enriched at high concentrations in the lung, has shown potent in vitro activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The aim of this study was to better understand the effectiveness of low-dose emetine for patients with coronavirus disease 2019 (COVID-19).Methods:In this real-world study, 63 patients with mild or common COVID-19 were recruited from Wuhan Fangcang Shelter Hospital and five COVID-19-designated hospitals in Anhui Province, China from February to March 2020. Thirty-nine patients from Wuhan Fangcang Shelter Hospital were assigned to a pragmatic randomized controlled clinical trial, and 24 patients from the 5 COVID-19-designated hospitals in Anhui Province underwent a real-world study. The medication course of emetine was less than 10 days. The main symptoms and adverse reactions of all patients were observed and recorded. The primary outcome measure was the time required for a negative SARS-CoV-2 RNA result or the negative result rate on day 10. Secondary outcomes included axillary temperature, transcutaneous oxygen saturation, and respiratory frequency recovery. The study was approved by the Ethics Committee of The First Affiliated Hospital of Anhui Medical University on February 20, 2019 (approval No. PJ2020-03-19) and was registered with the Chinese Clinical Trial Registry on February 20, 2019 (registration number: ChiCTR2000030022).Results:The oxygen saturation values were higher in the treatment group than in the control group on the first day after enrollment for patients treated at Fangcang Shelter Hospital. The axillary body temperature, respiratory rate, and oxygen saturation among patients in Fangcang Shelter Hospital were related to the time effect but not to the intervention measures. The respiratory rate and oxygen saturation of patients in the Anhui designated hospitals were related to the intervention measures but not to the time effect. The axillary body temperature of patients in Anhui designated hospitals was related to the time effect but not to the intervention measures.Conclusion:Our preliminary study shows that low-dose emetine combined with basic conventional antiviral drugs improves clinical symptoms in patients with mild and common COVID-19 without apparent adverse effects, suggesting that moderately increased doses of emetine may have good potential for treatment and prevention of COVID-19.