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简介：·AIM:Toinvestigatethepossibleeffectsofintracameralbevacizumabonoxidativestressparametersandapoptosisincornealtissue.·METHODS:Intotal,30ratswereassignedrandomlyintothefollowingthreegroupsof10ratseach:ashamgroup(Group1;n=10),acontrolgroup[Group2;balancedsaltsolution(BSS)wasadministeredat0.01mL;n=10],andatreatmentgroup(Group3;bevacizumabwasadministeredat0.25mg/0.01mL;n=10).Thetotalantioxidantstatus(TAS)andthetotaloxidantstatus(TOS)inthecornealtissueandbloodsamplesweremeasured,andtheoxidativestressindex(OSI)wascalculated.Additionally,cornealtissuehistopathologywasevaluatedforcaspase-3and8stainingandapoptoticactivity.·RESULTS:Inthebloodsamples,theTAS,TOS,andOSIlevelswerenotsignificantlydifferent(allP>0.05).Comparedwiththeshamandcontrolgroups,theTOSandOSIlevelsinthecornealtissuesweresignificantlydifferentinthebevacizumabgroup(allP<0.05).Nostatisticallysignificantdifferenceswereobservedbetweentheshamandcontrolgroups(allP>0.05).However,comparedwiththeshamandcontrolgroups,greaterimmunohistochemicalstainingforcaspases-3and8andanelevatedlevelofapoptoticactivitywereobservedinthebevacizumabgroup.·CONCLUSION:Thisstudyrevealedthatintracameralbevacizumabinjectionsseemedtobesystemicallysafebutmayhaveelicitedlocaltoxiceffectsinthecornealtissue,asindicatedbytheoxidativestressparametersandhistopathologicalevaluations.

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简介：backgroundToinvestigatetheeffectofintra-aorticballoonpumping(IABP)onno-reflowphenomenoninprimarypercutaneouscoronaryintervention(PCI)forST-ElevationMyocardialInfarction(STEMI).MethodsClinicaldataof22acutemyocardialinfarctionpatientsafterPCIwithangiographicno-reflowphenomenonwereretrospectivelyanalyzedbetweenJanuary2006andDecember2009.12patientsunderwentIABP,other10patientsascontrolgroup.Weobserveddifferenceofcardiacstructure,brainnatriureticpeptide(BNP)andventricularsystolicfunctionbetweentwogroup,aswellascardiacinjurymarkers(MYO,CK-MB,cTnI)inbothgroupsonthedaysof1,2,3,5,7,10afterthedifferentinterventions.Inaddition,cardiacstructureandventricularsystolicfunctionincludingleftatriummedialdiameter(LAMD),leftventricularmedialdiameter(LVMD),leftventricularejectionfraction(LVEF)wasevaluatedafter10days,3months,6months.Finally,statisticswasusedtoanalysisthedata.ResultsTheseveralvasoactivesubstancesaswellascardiacinjurymarkersandLAMD,LVMD,LVEFof10days,3months,6monthsofIABPgroupweresignificantdifferencewithcontrolgroup(P<0.05).BNPtargetsofIABPgroupcomparedwiththecontrolgroupnosignificantdifference(P>0.05).ConclusionsIABPhaseffectsonprognosisinSTEMIpatientswhoperformedPCIwithangiographicno-reflowphenomenon,whichisconducivetorecoveryofheartfunction.

简介：客观：为了探索完整的自动动物的可行性，建立动物的试验性的舱在normobaric/hypobarichypoxic和高二氧化碳环境当模特儿。方法：60只SPF班男SD老鼠被划分成二个组，20为normobaric，hypoxic条件和其它40为比重低於脑脊髓液，hypoxic条件。为每个组，我们检验了肺的动脉的压力和颈动脉由使用生理的多察觉者测量的老鼠的动脉的压力指示物，并且在结构观察了肺的脉管的变化。结果:有高二氧化碳环境的normobaric/hypobarichypoxic能支持肺的高血压的形成并且在肺的脉管的改变加速变化，并且支持恰好室的肥大。结论：临床的应用证明试验性的舱精确地观察了并且控制的动物。结果安全、可靠、可再现。舱能成功地与高二氧化碳环境在normobaric/hypobarichypoxic建立肺的高血压模型，并且由氧的缺乏引起以便学习许多循环和呼吸疾病的生理的机制，它为临床的研究提供了一个试验性的技术平台。

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简介：Inthepresentpaper,theauthorsreviewrecentsituationsofdisordersofthedigestivesystemanditsrelatedresearchprogresses.Inclinicalpractice,Neiguan(PC6)isusuallyselectedasoneofthemainintra-gastricexamination,cesareansection,etc.ConcerningexperimentalindicatedthatNeiguan(PC6)isanimportantandeffectiveacupointfortreatmentofgastrointestinaldisordersinclinic.StimulationofNeiguan(PC6)inducedfavorableregulationofboththeperipheralnervoussystemandcentralnervoussystem,andchangesofthegastrointestinalhormonesecretionmaycontributetoitseffectsintreatingvariousdisorders.

简介：AIMDevelopmentaltoxicitytestingtocurrentinternationalguidelinesinvolvesexposureofpregnantanimals,mostlyratsandrabbits,andsubsequentassessmentoftoxiceffectsintheirfetuses.Alternativemethodshavebeendevelopedsince1980s.Themostcomplexassaysinthisareamakedistinctiveandextensiveuseofisolatedpostimplantationrodentembryoswhichareculturedinvitroduringthephaseofmajororganogenesis.Theobjectiveofthisstudyistoestablishaninvitroembryotoxicitytest----basedonpostimplatation9.5dwholeembryoculture(WEC)and3T3celltoxicity.Thepossibilitiesfortheirapplicationasembryotoxicityscreenshavebeeninvestigated.

简介：Objective:ThisstudyaimstoestablishamethodforhighlyparallelmultiplexeddetectionofgeneticmutationsinChineselungcancersamplesthroughAgenaiPLEXchemistryandmatrix-assistedlaserdesorptionionizationtime-of-flightanalysisonMassARRAYmassspectrometryplatform.Methods:Wereviewedtherelatedliteratureanddataonlungcancertreatments.Wealsoidentified99mutationhotspotsin13targetgenescloselyrelatedtothepathogenesis,drugresistance,andmetastasisoflungcancer.Atotalof297primers,composedof99pairedforwardandreverseamplificationprimersand99matchedextensionprimers,weredesignedusingAssayDesignsoftware.Thedetectionmethodwasestablishedbyanalyzingeightcelllinesandsixlungcancerspecimens.TheproposedmethodwasthenvalidatedthroughcomparisonsbyusingaLungCarta~(TM)kit.ThesensitivityandspecificityoftheproposedmethodwereevaluatedbydirectlysequencingEGFRandKRASgenesin100lungcancercases.Results:Theproposedmethodwasabletodetectmultiplexgeneticmutationsinlungcancercelllines.Thisfindingwasconsistentwiththeobservationsonpreviouslyreportedmutations.Theproposedmethodcanalsodetectsuchmutationsinclinicallungcancerspecimens.ThisresultwasconsistentwiththeobservationswithLungCarta~(TM)kit.However,anFGFR2mutationwasdetectedonlythroughtheproposedmethod.Themeasuredsensitivityandspecificitywere100%and96.3%,respectively.Conclusions:TheproposedMassARRAYtechnology-basedmultiplexmethodcandetectgeneticmutationsinChineselungcancerpatients.Therefore,theproposedmethodcanbeappliedtodetectmutationsinothercancertissues.

简介：BackgroundHospital-acquiredpneumonia(HAP)isthemostcommonandmostseriousnosocomialinfectionforcardiacsurgerypatients,withhighincidenceandfatalityrate1.ItisimportantforcardiacsurgeonstocorrectlyidentifyHAP,assesstheseverity,andthenadjustanti-infectionmethod,whichcanreducethemortalityrate,shortenhospitalizationtime,andreducethewasteofmedicalresources.ThepurposeofthisresearchistoevaluatetheapplicationvalueoflungCTindiagnosisandtreatmentofHAPaftercardiacsurgery.MethodsAretrospectiveanalysiswasconductedforclinicaldataabout76cardiacsurgerypatientswhowerediagnosedwithHAPduringJanuarytoDecember2013.Theclinicaldatamainlyincludedsymptoms,physicalsigns,laboratoryexaminations(suchasroutinebloodtestsandserumprocalcitonin),andlungCTandX-raydata.OurfocusisonthecomparisonbetweenlungCTandX-raydata.ResultsThepositivediagnosticrate,falsenegativerate,andfalsepositiverateoflungCTwere71/76(93.4%),5/76(6.6%),and1/76(1.3%)respectively.ThecoincidencerateofX-rayandCTwas45/76(59.2%),andthefalsenegativerateofX-raywas23/76(30.3%).ConclusionLungCTisbetterthanX-rayindiagnosisofHAPaftercardiacsurgeryandassessmentofseverity,andhasgreatersignificanceforguidingtherationaluseofantibiotics.Therefore,lungCTisworthyofapplicationandpopularization.

简介：Thesubcutaneousimplantablecardioverter-defibrillator(ICD)isanoveltechnologyusingasubcutaneous(extrathoracic)systemfortreatmentofpotentiallethalventriculararrhythmias.ItavoidsmanyoftherisksoftransvenousICDimplantation.ItmaybeconsideredinpatientshavinganICDindicationwhodonothaveapacingand/orcardiacresynchronizationtherapyindication,andwhoareunlikelytobenefitfromantitachycardiapacingtherapy.Wereviewpatientselection,systemcomponents,theimplantationtechnique,andscreeningconsiderationsforsubcutaneousICDimplantation.Itsusesinspecificpatientpopulations,includingchildren,patientswithcongenitalheartdisease,hypertrophiccardiomyopathy,orend-stagerenaldisease,andpatientswithpreexistingpacemakers,arehighlighted.Areasoffutureinvestigationarereviewed,includingpotentialusewithleadlesspacingandmagneticresonanceimaging.

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简介：AbstractSince severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified during late 2019, the sustained spread of this pathogen within the human population has caused worldwide disruption with staggering infection rates and death tolls. Due to the accumulation of mutations in SARS-CoV-2, the virus has evolved into many variants, five of which have been listed as variants of concern VOCs by the World Health Organization (WHO). Multiple animal models of SARS-CoV-2 have been developed to evaluate vaccines and drugs and to assess the pathogenicity, transmissibility and antiviral measures of these VOCs. Here, we review the cutting-edge research based on mouse, hamster, ferret and non-human primate models for evaluating SARS-CoV-2 with a focus on the Omicron variant, and highlight the importance of updating vaccines in a timely manner in order to mitigate the negative effects of SARS-CoV-2 infections in the human population.

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简介：Prematureventricularcontraction(PVC)isthemostfrequentarrhythmiaencounteredinclinicalpractice.PVCmayoccurinhealthsubjects,whichisnotimminentlylife-threateningbutmayrequiretherapiestopreventfurtherproblems.So,thetimelyPVCrecognitionbecomesveryimportantfortheanalysisofelectrocardiogram(ECG),especiallyfortheremoteECGmonitoringusingmobilephones.Inthispaper,aconstructionmethodofpersonalizedECGtemplateandaPVCrecognitionmethodbasedontemplatematchingwerestudied.Firstly,weselected43ECGrecordingsfromtheMIT-BIHarrhythmiadatabase.Allrecordingsweredividedintotwodatasets(DS1fortrainingandDS2fortesting)andeachdatasetapproximatelycontainedthesameproportionofPVCbeats.Subsequently,foreachrecording(30min)inDS1,thefirst5minrecordingswereusedtoconstructthepersonalizedECGtemplateandthelast25minrecordingswereusedfortheR-wavepeaksdetectionandPVCrecognition,wherethetemplatematchingmethodwereused.ThevalidityoftheproposedmethodswastestedusingDS2.Theresultsshowedthat:1)highbeatdetectionaccuracywasachievedforbothPVCbeatsandnon-PVCbeats;2)thesensitivityandspecificityofPVCrecognitionwere99.11%and99.96%forthefirst5minrecordingsrespectively,99.17%and99.43%forthelast25minrecordingsrespectively.Alltheproposedmethodscanbereal-timeperformed,whichshowapromisingprospectfortheapplicationofECGmobilephones.