摘要
Immatureembryosofricevarieties“Xiushui11”and“Chunjiang11”preculturedfor4dwereinfectedandtransformedbyAgrobacteriumtumefaciensstrainEHA101/pExT7(containingthespiderinsecticidalgene).Theresistantcalliweretransferredontothedifferentiationmediumandplantswereregenerated.Thetransformationfrequencyreached56%~72%measuredasnumbersofGeneticin(G418)-resistantcalliproducedand36%~60%measuredasnumbersoftransgenicplantsregenerated,respectively.PCRandSouthernblotanalysisoftransgenicplantsconfirmedthattheT-DNAhadbeenintegratedintothericegenome.InsectbioassaysusingT1transgenicplantsindicatedthatthemortalityoftheleaffolder(Cnaphalocrasismedinalis)after7dofleaffeedingreached38%~61%andthecorrectedmortalityofthestripedstemborer(Chilosuppressalis)after7dofleaffeedingreached16%~75%.Theinsectbioassayresultsdemonstratedthatthetransgenicplantsexpressingthespiderinsecticidalproteinconferredenhancedresistancetothesepests.
出版日期
2001年02月12日(中国期刊网平台首次上网日期,不代表论文的发表时间)