摘要
SeveralimportantfactorsaffectingtheefficiencyofAgrobacterium-mediatedricetransformationwerestudiedwithseveralpredominantcommercialindicaandjaponicaricecultivars.Asfarasindicaricecalluswasconcerned,CCmediumwasthebestandthequalityofcalluswasimprovedwiththeadditionof1.0to2.0mg/LABA.Itdecreasedthepercentageofbrowningcalliandimprovedthecallusgrowingstatebyadditionofacertainamountofsorbitoltothesubculturemedium.NBmediumwasthebestforcallusinitiationofjaponicarice,buttheimprovementinthequalityofcallusofjaponicasubspecieswasnotobviousbyaddingABA.Duringtheperiodofsubculture,toacertaindegree,increasingthesucroseconcentrationcouldimprovetheproportionofhygromycinresistantcalli.Furthermore,thetransformationefficiencywouldbehigherbyapplyingselectionpressureintheselectionstage,removingselectionpressureduringtheplantletdifferentiationperiodandapplyingselectionpressureagainduringseedlinghardeningperiod.Besides,suitablecombinationofplanthormoneswasbeneficialforcallusdifferentiation.AnefficientAgrobacterium-mediatedricetransformationsystemhadbeenestablishedforseveralricecultivarsandalotoftransgenicriceplantshadbeenobtained.
出版日期
2004年04月14日（中国期刊网平台首次上网日期，不代表论文的发表时间）
