摘要
Fluorescenceliftimeimaging(FLIM)ofmodifiedhydrophobicbodipydyesthatactasfluorescentmolecularrotorsshowsthatthefluorescencelifetimeoftheseprobesisafunctionofthemicroviscosityoftheirenvironment.Incubatingcellswiththesedyes,wefindapunctateandcontinuousdistributionofthedyeincells.Theviscosityvalueobtainedinwhatappearstobeendocytoticvesiclesinlivingcellsisaround100timeshigherthanthatofwaterandofcellularcytoplasm.Time-resolvedfluorescenceanisotropymeasurementsalsoyieldrotationalcorrelationtimesconsistentwithlargemicroviscosityvalues.Inthisway,wesuccessfullydevelopapracticalandversatileapproachtomapthemicroviscosityincellsbasedonimagingfluorescentmolecularrotors.
出版日期
2010年10月20日（中国期刊网平台首次上网日期，不代表论文的发表时间）
